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The map label for this gene is aksA

Identifier: 17230460

GI number: 17230460

Start: 3609625

End: 3610755

Strand: Direct

Name: aksA

Synonym: alr2968

Alternate gene names: 17230460

Gene position: 3609625-3610755 (Clockwise)

Preceding gene: 17230459

Following gene: 17230464

Centisome position: 56.28

GC content: 43.41

Gene sequence:

>1131_bases
ATGAATAAAGTTCTAATTAATGACACTACACTACGCGATGGAGAGCAAGCGGCTGGTGTTGCTTTTAGCGTAGAGGAAAA
AATAGCGATCGCCAAATTTCTCGATGCAATTGGTGTGCATGAAATCGAAGTGGGAATACCCGCAATGGGTAAAGCAGAAC
AAGAGGCGATCGCTAATATTGTGAAGTTAGATTTGTCAGCAAATTTACTTGGTTGGAACCGCGCCGTGATTGCTGATATT
CAGGCTTCCATCGCTTGTGGTTTACAGCGAGTACATATCTCTATTCCCGTTTCTGCAATCCAAATCGCCGTCAAATTTCA
CGGACAATGGCAAGTAGTTTTACAAAAACTCCACGATAGTATTAGCTTTGCTGTTGACCAAGGTTTATTTGTCTCCATCG
GTGGTGAAGACTCATCAAGAGCCGAAGAAAGCTTCCTCCTTGATGTAGTGCTAGCTGCTCAAGAATGGGGTGCGTCACGA
TTTCGCTTTTGCGACACAGTAGGTATTCTTGACCCGTTCACTACCCACGCCAAGGTAAAACAATTGGTGGCATCTTTGAC
AATACCTGTAGAAATGCACACCCACAATGATTTTGGTTTGGCAACAGCCAACGCTCTGGCAGGTACCAAAGCAGGCGCTT
TATCTGTAAATACTACTGTGAATGGATTAGGTGAAAGAGCCGGGAATGCTGCTTTAGAAGAGGTGGTTATGGCTCTCAAG
CACTTGTATCACCATGACTTAGGAATTGATACACGGCGTTTACTGGAAATATCTCAACTCGTAGCATCAGCATCAGGTCA
TCCTGTACCACCTTGGAAAGCAATTGTTGGTGAAAATACTTTTGCTCACGAATCCGGGATTCACGCTCATGGTGTACTGC
AAAATCCTCAAACCTACGAGCCATTTGCACCAGAAGAAGTAGGGAGAGAGCGCCGTTTAGTTGTGGGTAAACATTCTGGT
AGACATTTACTCTCTAGTATTTTGCAGCAACACGACATTATTCTCAATCACGAAGAAACCCAATTTGTCTTAGATGCAGT
TAGACAAGAATCAGTAGAAAAAAAACGCAGCCTCACAGATCAAGAACTTTTGCATTTAGTGCAGACAAAACAACATTTTC
GTGCTTGTTGA

Upstream 100 bases:

>100_bases
CAATTCCTTTACTTTTAGAGTATCCTTTGTCAGATTACCTTGGCACTTAGCCAGACTTAGGCGAAGAATCTACCTTTGTT
TCCTCGGAGACACTATGCAA

Downstream 100 bases:

>100_bases
ATGAGTTTATTAGGATTCTCCAGGACTTACGCAACTGGTATATTAGTAGGATGCGTCAGGTATCAAGAATCTGTTTATTG
ACAGCATTCGAGCCGTCTGA

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MNKVLINDTTLRDGEQAAGVAFSVEEKIAIAKFLDAIGVHEIEVGIPAMGKAEQEAIANIVKLDLSANLLGWNRAVIADI
QASIACGLQRVHISIPVSAIQIAVKFHGQWQVVLQKLHDSISFAVDQGLFVSIGGEDSSRAEESFLLDVVLAAQEWGASR
FRFCDTVGILDPFTTHAKVKQLVASLTIPVEMHTHNDFGLATANALAGTKAGALSVNTTVNGLGERAGNAALEEVVMALK
HLYHHDLGIDTRRLLEISQLVASASGHPVPPWKAIVGENTFAHESGIHAHGVLQNPQTYEPFAPEEVGRERRLVVGKHSG
RHLLSSILQQHDIILNHEETQFVLDAVRQESVEKKRSLTDQELLHLVQTKQHFRAC

Sequences:

>Translated_376_residues
MNKVLINDTTLRDGEQAAGVAFSVEEKIAIAKFLDAIGVHEIEVGIPAMGKAEQEAIANIVKLDLSANLLGWNRAVIADI
QASIACGLQRVHISIPVSAIQIAVKFHGQWQVVLQKLHDSISFAVDQGLFVSIGGEDSSRAEESFLLDVVLAAQEWGASR
FRFCDTVGILDPFTTHAKVKQLVASLTIPVEMHTHNDFGLATANALAGTKAGALSVNTTVNGLGERAGNAALEEVVMALK
HLYHHDLGIDTRRLLEISQLVASASGHPVPPWKAIVGENTFAHESGIHAHGVLQNPQTYEPFAPEEVGRERRLVVGKHSG
RHLLSSILQQHDIILNHEETQFVLDAVRQESVEKKRSLTDQELLHLVQTKQHFRAC
>Mature_376_residues
MNKVLINDTTLRDGEQAAGVAFSVEEKIAIAKFLDAIGVHEIEVGIPAMGKAEQEAIANIVKLDLSANLLGWNRAVIADI
QASIACGLQRVHISIPVSAIQIAVKFHGQWQVVLQKLHDSISFAVDQGLFVSIGGEDSSRAEESFLLDVVLAAQEWGASR
FRFCDTVGILDPFTTHAKVKQLVASLTIPVEMHTHNDFGLATANALAGTKAGALSVNTTVNGLGERAGNAALEEVVMALK
HLYHHDLGIDTRRLLEISQLVASASGHPVPPWKAIVGENTFAHESGIHAHGVLQNPQTYEPFAPEEVGRERRLVVGKHSG
RHLLSSILQQHDIILNHEETQFVLDAVRQESVEKKRSLTDQELLHLVQTKQHFRAC

Specific function: This protein is a Fe-Mo-cofactor biosynthetic component

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family

Homologues:

Organism=Escherichia coli, GI1786261, Length=396, Percent_Identity=33.5858585858586, Blast_Score=182, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6320071, Length=347, Percent_Identity=28.2420749279539, Blast_Score=146, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6320019, Length=354, Percent_Identity=27.683615819209, Blast_Score=145, Evalue=1e-35,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NIFV2_NOSS1 (P58637)

Other databases:

- EMBL:   BA000019
- PIR:   AI2176
- RefSeq:   NP_487008.1
- ProteinModelPortal:   P58637
- SMR:   P58637
- STRING:   P58637
- GeneID:   1106566
- GenomeReviews:   BA000019_GR
- KEGG:   ana:alr2968
- NMPDR:   fig|103690.1.peg.3275
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   VEMHTHN
- PhylomeDB:   P58637
- ProtClustDB:   PRK11858
- BioCyc:   NSP103690:ALR2968-MONOMER
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR02660

Pfam domain/function: PF00682 HMGL-like

EC number: =2.3.3.14

Molecular weight: Translated: 40937; Mature: 40937

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVLINDTTLRDGEQAAGVAFSVEEKIAIAKFLDAIGVHEIEVGIPAMGKAEQEAIANI
CCCEEECCCCCCCCCHHCCEEEEHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHH
VKLDLSANLLGWNRAVIADIQASIACGLQRVHISIPVSAIQIAVKFHGQWQVVLQKLHDS
HHHCCCCCEECCCHHHHHHHHHHHHHCEEEEEEECCHHHEEEEEEECCCHHHHHHHHHHH
ISFAVDQGLFVSIGGEDSSRAEESFLLDVVLAAQEWGASRFRFCDTVGILDPFTTHAKVK
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHH
QLVASLTIPVEMHTHNDFGLATANALAGTKAGALSVNTTVNGLGERAGNAALEEVVMALK
HHHHHHCCEEEEECCCCCCCCHHHHHCCCCCCEEEECCHHHHHHHHCCHHHHHHHHHHHH
HLYHHDLGIDTRRLLEISQLVASASGHPVPPWKAIVGENTFAHESGIHAHGVLQNPQTYE
HHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCEECCCCCCCC
PFAPEEVGRERRLVVGKHSGRHLLSSILQQHDIILNHEETQFVLDAVRQESVEKKRSLTD
CCCHHHHCCCCEEEEECCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHCCCH
QELLHLVQTKQHFRAC
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKVLINDTTLRDGEQAAGVAFSVEEKIAIAKFLDAIGVHEIEVGIPAMGKAEQEAIANI
CCCEEECCCCCCCCCHHCCEEEEHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHH
VKLDLSANLLGWNRAVIADIQASIACGLQRVHISIPVSAIQIAVKFHGQWQVVLQKLHDS
HHHCCCCCEECCCHHHHHHHHHHHHHCEEEEEEECCHHHEEEEEEECCCHHHHHHHHHHH
ISFAVDQGLFVSIGGEDSSRAEESFLLDVVLAAQEWGASRFRFCDTVGILDPFTTHAKVK
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHH
QLVASLTIPVEMHTHNDFGLATANALAGTKAGALSVNTTVNGLGERAGNAALEEVVMALK
HHHHHHCCEEEEECCCCCCCCHHHHHCCCCCCEEEECCHHHHHHHHCCHHHHHHHHHHHH
HLYHHDLGIDTRRLLEISQLVASASGHPVPPWKAIVGENTFAHESGIHAHGVLQNPQTYE
HHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCEECCCCCCCC
PFAPEEVGRERRLVVGKHSGRHLLSSILQQHDIILNHEETQFVLDAVRQESVEKKRSLTD
CCCHHHHCCCCEEEEECCCCHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHCCCH
QELLHLVQTKQHFRAC
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840