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The map label for this gene is ntrB [H]

Identifier: 17230448

GI number: 17230448

Start: 3597005

End: 3598654

Strand: Reverse

Name: ntrB [H]

Synonym: all2956

Alternate gene names: 17230448

Gene position: 3598654-3597005 (Counterclockwise)

Preceding gene: 17230452

Following gene: 17230447

Centisome position: 56.11

GC content: 39.39

Gene sequence:

>1650_bases
GTGACGCTTATGCTTCTTAATCGCTTCTGTCTTTCATTAAATGCTGATTTTACAAAGGTTTTAGTCAAGCGGAAACCCCA
ACTCTCCACATGGTCTATCTTGTTAAGTGCAATTATAATTGCGATCGCCAGTGCTGCTACTATTTATGAACTCAATCAGT
TAGCAGAACGCAGCAATGACAAGAGATTGCTTTTGACTCGATTAAAAGAGCAAGTGAGCAGACTTAACGCCTTAGAATGG
GAGGGCATTTCCAAAGGCGAAATTGATGAGGATTTAACCGAAGAGCTGGCAGAAAATAAGCAAAGTACAGATAGCATTTT
GCAGCAGCTACGACAAATTAAACCACAAAAAAATCTGGACAATATTTTTGCCCTTCATACCAGCTATCAACAAGCAATTG
ACAACGCTTTACAGCTCGTTGCTCAAGGCAAAACCAAAGAAGCGATAAATGTTGATGCAGATGGCATTGATGAAATCTAT
GATGATTTATACGCTGAAGTAACAACCCTGGAAGAAGTCTACGTAGCGCAGAAGCAACAGACTAGACAATTTGCAGATAT
TGGCACTACTTTTTGTTTAATATTCGCATCTGTTATTATTGGTGCTTTGTCATACAAGTACAGCAAAAGCTTGTTGAACA
AAAACCAACAGTTAGAAGCTGCTTTCCAAGAACTCAAACAAACCCAAGATCAATTGATTCAACAAGAAAAAATGGCAGCC
TTAGGACAACTGGTTGCGGGAGTTGCCCATGAAATCAATAATCCCTTGGGTATTATTCAAGCATCAGCGACTAACACAGA
ACAAGCCCTGCAAGCAGCCTTGACTGAGCTTCCCTCTTTACATCAGCGTTTGAATTCCCAAGAACAAGAAAGCTTTTTTC
AACTCATTAATCAAGGATTAAAAAACCAACCTACGACACTTTTTCAACCAGACCGTTCACTTAAACGTAAAATTACTGCT
CATCTGCAAGAGCATAACATTGAAAATGCTAGAGATATTGCTGATCTCTTGATAGATATGGGCATTGCTGAAGATTGGGA
ATCTTTACTGCCTATGTTAAAAAGTGATCATGGTGAATGGGCTTTACAGTTCGCTTATGACCTAAGTTGCTGCTTTAGCA
ATAATCAGATGATTCTCAATGCTGTTGATCGTTCTGCTAAAATCGTTTTTGCGCTTAAAAGCTATTCTCATTTTGATCAA
AGTGGCAATAAGCAGTTGATGCAGGTAACTACAGGACTAGAAACTACCATTGCCATCTATCAGAATCAACTGAAACGCAA
TATTCATTTAATTCGAGATTACCAGGATGTTCCCGACATTTGGGGATACCCTGATGAACTCATTCAAGTCTGGACAAATC
TTATTTTCAATGCGATTCAAGCAATGGAATCAGGTGGGACACTGACCATTGCTATTCGTAAAGCAGAAGAATACGTTGAA
GTCAGCATTACAGACACCGGATTGGGCATTCCTCCAGAAGTGCAGCAAAAAATCTTTGATGCCTTTTTTACCACCAAACC
TGCTGGAGAAGGCAGTGGTCTGGGATTACACATTTGCAAAAAAATTCTTGACAAACATCAAGGACAGATCAAAATGGAGA
GCAATCCAGGTCATACTCAATTCAGTGTTTGCTTGCCCATATTAACTTAG

Upstream 100 bases:

>100_bases
GTGGTATGGGTTACATTCTTTGGCTGTCGGCGTTTTGGTTTATGGCGATCGCTAGTACTACCAAGACTAAGTTGCCACAA
TTTCCTCTTAAGTATTATTG

Downstream 100 bases:

>100_bases
ATAACAAGAGGAAATTTAATTTATGTCTAATGCTGCTATTCTCTGTGTAGATGATGAAGTTTCTATATTAAAAGCTCTCA
AGGAACAACTCAAAAGGTTA

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MTLMLLNRFCLSLNADFTKVLVKRKPQLSTWSILLSAIIIAIASAATIYELNQLAERSNDKRLLLTRLKEQVSRLNALEW
EGISKGEIDEDLTEELAENKQSTDSILQQLRQIKPQKNLDNIFALHTSYQQAIDNALQLVAQGKTKEAINVDADGIDEIY
DDLYAEVTTLEEVYVAQKQQTRQFADIGTTFCLIFASVIIGALSYKYSKSLLNKNQQLEAAFQELKQTQDQLIQQEKMAA
LGQLVAGVAHEINNPLGIIQASATNTEQALQAALTELPSLHQRLNSQEQESFFQLINQGLKNQPTTLFQPDRSLKRKITA
HLQEHNIENARDIADLLIDMGIAEDWESLLPMLKSDHGEWALQFAYDLSCCFSNNQMILNAVDRSAKIVFALKSYSHFDQ
SGNKQLMQVTTGLETTIAIYQNQLKRNIHLIRDYQDVPDIWGYPDELIQVWTNLIFNAIQAMESGGTLTIAIRKAEEYVE
VSITDTGLGIPPEVQQKIFDAFFTTKPAGEGSGLGLHICKKILDKHQGQIKMESNPGHTQFSVCLPILT

Sequences:

>Translated_549_residues
MTLMLLNRFCLSLNADFTKVLVKRKPQLSTWSILLSAIIIAIASAATIYELNQLAERSNDKRLLLTRLKEQVSRLNALEW
EGISKGEIDEDLTEELAENKQSTDSILQQLRQIKPQKNLDNIFALHTSYQQAIDNALQLVAQGKTKEAINVDADGIDEIY
DDLYAEVTTLEEVYVAQKQQTRQFADIGTTFCLIFASVIIGALSYKYSKSLLNKNQQLEAAFQELKQTQDQLIQQEKMAA
LGQLVAGVAHEINNPLGIIQASATNTEQALQAALTELPSLHQRLNSQEQESFFQLINQGLKNQPTTLFQPDRSLKRKITA
HLQEHNIENARDIADLLIDMGIAEDWESLLPMLKSDHGEWALQFAYDLSCCFSNNQMILNAVDRSAKIVFALKSYSHFDQ
SGNKQLMQVTTGLETTIAIYQNQLKRNIHLIRDYQDVPDIWGYPDELIQVWTNLIFNAIQAMESGGTLTIAIRKAEEYVE
VSITDTGLGIPPEVQQKIFDAFFTTKPAGEGSGLGLHICKKILDKHQGQIKMESNPGHTQFSVCLPILT
>Mature_548_residues
TLMLLNRFCLSLNADFTKVLVKRKPQLSTWSILLSAIIIAIASAATIYELNQLAERSNDKRLLLTRLKEQVSRLNALEWE
GISKGEIDEDLTEELAENKQSTDSILQQLRQIKPQKNLDNIFALHTSYQQAIDNALQLVAQGKTKEAINVDADGIDEIYD
DLYAEVTTLEEVYVAQKQQTRQFADIGTTFCLIFASVIIGALSYKYSKSLLNKNQQLEAAFQELKQTQDQLIQQEKMAAL
GQLVAGVAHEINNPLGIIQASATNTEQALQAALTELPSLHQRLNSQEQESFFQLINQGLKNQPTTLFQPDRSLKRKITAH
LQEHNIENARDIADLLIDMGIAEDWESLLPMLKSDHGEWALQFAYDLSCCFSNNQMILNAVDRSAKIVFALKSYSHFDQS
GNKQLMQVTTGLETTIAIYQNQLKRNIHLIRDYQDVPDIWGYPDELIQVWTNLIFNAIQAMESGGTLTIAIRKAEEYVEV
SITDTGLGIPPEVQQKIFDAFFTTKPAGEGSGLGLHICKKILDKHQGQIKMESNPGHTQFSVCLPILT

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=146, Percent_Identity=36.3013698630137, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790436, Length=131, Percent_Identity=36.6412213740458, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788549, Length=103, Percent_Identity=41.747572815534, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI145693157, Length=118, Percent_Identity=36.4406779661017, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1786783, Length=111, Percent_Identity=32.4324324324324, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788393, Length=147, Percent_Identity=30.6122448979592, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789149, Length=110, Percent_Identity=37.2727272727273, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788713, Length=112, Percent_Identity=33.9285714285714, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 61696; Mature: 61565

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEECCCCC
>Mature Secondary Structure 
TLMLLNRFCLSLNADFTKVLVKRKPQLSTWSILLSAIIIAIASAATIYELNQLAERSND
HHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC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EEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8095910 [H]