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The map label for this gene is pbpF [H]

Identifier: 17230444

GI number: 17230444

Start: 3592495

End: 3594762

Strand: Reverse

Name: pbpF [H]

Synonym: all2952

Alternate gene names: 17230444

Gene position: 3594762-3592495 (Counterclockwise)

Preceding gene: 17230447

Following gene: 17230443

Centisome position: 56.05

GC content: 45.86

Gene sequence:

>2268_bases
GTGGTAAGACAAAAAAAATTATTAAACCAGATAAGTGGTATAACATCTGGGATTGTTACTAGGTTTACAAGCCGTGAACA
CCCAATTTATCGCCGTGTATGGTTTTGGGCGGGCTTAAGTGTAGGTGGTAGCATCATTGCCTTCAATTACTTATTAGGGG
AAATCGATAAAACCTTACCTGAGAAAGCGGAATTGCAAGCTGTCGTCCGTGAGCAAACAATTACCATCAAAGCTGTTGAT
GGCACTATATTACAACAGCAAGGCGAAGCAACCAGGGAAAAAGTCAATCTGGAGCAGATGCCGGAGATCCTCAAAAATGC
TTTTATTGCCTCCGAAGACAGCAGATTCACTCAGCACCAAGGTATTGACCCCCAAGGAATTGTCAGAGCAGCCGTAAATA
ATTTGCGATCGCAAAATGTGGTAGAAGGTGGTAGCACGATTACTCAACAGTTATCACGGATTCTTTTCCTCAAACAAGAG
CGTACAGTTTGGCGCAAACTTAAGGAAGTACGCCTTGCCCAAAAGCTAGAACAAGAATTAACCAAAGACCAGATTTTAGA
GCGTTATTTGAATTTAGTATATTTAGGTGCTGGGGCTTATGGTGTAGCAGATGCAGCCTGGGTATACTTCAGTAAACCAG
TAGACCAACTTACCCTCCCGGAAGCGGCAACAATCGCGGGAATGCCTCCTGCACCGAGCTTATTTTCACCAGACAAAAAC
CCGCAAGCAGCCAAACAACGACGAAATTTGGTATTGCAACGGATGGAAAAAGAGGGCTTTATTACACCAGCCCAAAGGCA
AGCAGCCACCCAAGCAGTAATTACCCTCAAATCTAGTTTACCCAAGCGGTTACAAGTAGAATCCCCCTACTTCACCAGCT
ATATTCAGCAAGAATTGCCCAAGTATGTTTCTTCGGAAGTGTTAGCGAATGGGGGTTTAACTGTGGAAACCAGCTTAAAC
CCAACTTGGCAGCAAGCCGCAGAAGCAGCCGTTGCTAAAACTTTACGCAATCAAGGACGATGGCAAAACTTCAAACAAGC
CGCTTTAGTTGCCATCGATCCCCGTAATGGGGAAATCAAGGCAATGGTTGGGGGTCAAGACTTTGGTCAAAACCAATTTA
ACCGTGTTACCCAAGCGCAACGCCAGCCAGGTTCCACATTTAAGGGTTTTGTCTATGCGACAGCTATCGCCACTGGTAAA
AACCCCAACGATACTTATGTAGATGCTCCCGTTGTAGTCGATGGTTACGAACCGAAAAACTACGGGGAAACCTTCCGAGG
CACAATGAATATGCGCGATGCCCTCACCCGTTCGGTGAATGTGGTCGCGTTGAAAATCATGCTAGATGTGGGATTTGAGC
CAACAATTCAACTAGCCAAGAACATGGGGATTAAATCAAAACTCAATCCTCATTATTCTCTAGCCCTTGGTTCCAATGAA
GTTAATCTCTTAGAGTTAACCAGTGCTTACGGCAGTTTTGCAACCCTTGGGTTACACTCGGAACCTCATGGCATCCGTCG
TATCCTCAACCGCAAAGGTGAGGTCATCTGGTCAGCAAAATTTGACTCGAAGCGAGTGCTTGATCCTACTAGTGCCGCTA
TTATGACCTGGATGTTACGTAACGTGGTCGAAGCAGGCACTGGTGCAGCCGCCCAATTGCCTAATAGACCCGTTGCTGGT
AAGACAGGTACATCTGATGAAGCCCGCGATTTATGGTTTATTGGCTATATTCCCCAAGTAGTTACAGGGGTTTGGTTGGG
TAACGATAATAATCGTCCTACTTATGGTAGTAGTGGCAGTGCTGCTTACACTTGGCATGAATTTATGGAAAAAGCCATAG
AGGGAATGCCAATAGAAAAATTTCCCGAAAGACCAAAACTGGAAGGTCGCAAGGGCAGCATCAAAGCAAAACCCATCAAA
GCAAAACAAATTCTCAATCGTTCTCTGACACCTAATGAAGAGTCAGCGCAAGAGACTGGTTCTTCCAGCAGACGCAGAAG
GCGCTCCCAACGATTAGAAGAAACCACTCCAACTGATTCACCTAGAAGACGTAGACGATATCGTAGCCAAGAATCAGGTT
CTAGTGGTAGTGAAGAATCACCCCGCACAAGACGGCGCAGACCAGTTGTAGAATCAGATACTTCACAACCGTCCCGCAGG
TCTCGACGGTCTTCATCCACAGTAGATAATAGCTCTGGCTCGGCCAGTTCTGCACCAACACCTTCTTGGCGGGAAAGACT
TAGACCTAATTCATCCTCTTCTAACTAA

Upstream 100 bases:

>100_bases
ACTAAATTTGAGTGACTCTGACAAGGGGGAACCACGCTTGCCACCTAGTAATCGTTACGAGCAAGAGGAAACCACAAACG
AGAACTTACCGCCAACTCAG

Downstream 100 bases:

>100_bases
CTTAGGTTGTGAGCTATTATAGCTGGGGACTGGGGACTGGGGACTGGGTGAAAAGTCTTGTTGTGTCTAGGTTGTATCAT
CTATATGATGTCCTAACACT

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 755; Mature: 755

Protein sequence:

>755_residues
MVRQKKLLNQISGITSGIVTRFTSREHPIYRRVWFWAGLSVGGSIIAFNYLLGEIDKTLPEKAELQAVVREQTITIKAVD
GTILQQQGEATREKVNLEQMPEILKNAFIASEDSRFTQHQGIDPQGIVRAAVNNLRSQNVVEGGSTITQQLSRILFLKQE
RTVWRKLKEVRLAQKLEQELTKDQILERYLNLVYLGAGAYGVADAAWVYFSKPVDQLTLPEAATIAGMPPAPSLFSPDKN
PQAAKQRRNLVLQRMEKEGFITPAQRQAATQAVITLKSSLPKRLQVESPYFTSYIQQELPKYVSSEVLANGGLTVETSLN
PTWQQAAEAAVAKTLRNQGRWQNFKQAALVAIDPRNGEIKAMVGGQDFGQNQFNRVTQAQRQPGSTFKGFVYATAIATGK
NPNDTYVDAPVVVDGYEPKNYGETFRGTMNMRDALTRSVNVVALKIMLDVGFEPTIQLAKNMGIKSKLNPHYSLALGSNE
VNLLELTSAYGSFATLGLHSEPHGIRRILNRKGEVIWSAKFDSKRVLDPTSAAIMTWMLRNVVEAGTGAAAQLPNRPVAG
KTGTSDEARDLWFIGYIPQVVTGVWLGNDNNRPTYGSSGSAAYTWHEFMEKAIEGMPIEKFPERPKLEGRKGSIKAKPIK
AKQILNRSLTPNEESAQETGSSSRRRRRSQRLEETTPTDSPRRRRRYRSQESGSSGSEESPRTRRRRPVVESDTSQPSRR
SRRSSSTVDNSSGSASSAPTPSWRERLRPNSSSSN

Sequences:

>Translated_755_residues
MVRQKKLLNQISGITSGIVTRFTSREHPIYRRVWFWAGLSVGGSIIAFNYLLGEIDKTLPEKAELQAVVREQTITIKAVD
GTILQQQGEATREKVNLEQMPEILKNAFIASEDSRFTQHQGIDPQGIVRAAVNNLRSQNVVEGGSTITQQLSRILFLKQE
RTVWRKLKEVRLAQKLEQELTKDQILERYLNLVYLGAGAYGVADAAWVYFSKPVDQLTLPEAATIAGMPPAPSLFSPDKN
PQAAKQRRNLVLQRMEKEGFITPAQRQAATQAVITLKSSLPKRLQVESPYFTSYIQQELPKYVSSEVLANGGLTVETSLN
PTWQQAAEAAVAKTLRNQGRWQNFKQAALVAIDPRNGEIKAMVGGQDFGQNQFNRVTQAQRQPGSTFKGFVYATAIATGK
NPNDTYVDAPVVVDGYEPKNYGETFRGTMNMRDALTRSVNVVALKIMLDVGFEPTIQLAKNMGIKSKLNPHYSLALGSNE
VNLLELTSAYGSFATLGLHSEPHGIRRILNRKGEVIWSAKFDSKRVLDPTSAAIMTWMLRNVVEAGTGAAAQLPNRPVAG
KTGTSDEARDLWFIGYIPQVVTGVWLGNDNNRPTYGSSGSAAYTWHEFMEKAIEGMPIEKFPERPKLEGRKGSIKAKPIK
AKQILNRSLTPNEESAQETGSSSRRRRRSQRLEETTPTDSPRRRRRYRSQESGSSGSEESPRTRRRRPVVESDTSQPSRR
SRRSSSTVDNSSGSASSAPTPSWRERLRPNSSSSN
>Mature_755_residues
MVRQKKLLNQISGITSGIVTRFTSREHPIYRRVWFWAGLSVGGSIIAFNYLLGEIDKTLPEKAELQAVVREQTITIKAVD
GTILQQQGEATREKVNLEQMPEILKNAFIASEDSRFTQHQGIDPQGIVRAAVNNLRSQNVVEGGSTITQQLSRILFLKQE
RTVWRKLKEVRLAQKLEQELTKDQILERYLNLVYLGAGAYGVADAAWVYFSKPVDQLTLPEAATIAGMPPAPSLFSPDKN
PQAAKQRRNLVLQRMEKEGFITPAQRQAATQAVITLKSSLPKRLQVESPYFTSYIQQELPKYVSSEVLANGGLTVETSLN
PTWQQAAEAAVAKTLRNQGRWQNFKQAALVAIDPRNGEIKAMVGGQDFGQNQFNRVTQAQRQPGSTFKGFVYATAIATGK
NPNDTYVDAPVVVDGYEPKNYGETFRGTMNMRDALTRSVNVVALKIMLDVGFEPTIQLAKNMGIKSKLNPHYSLALGSNE
VNLLELTSAYGSFATLGLHSEPHGIRRILNRKGEVIWSAKFDSKRVLDPTSAAIMTWMLRNVVEAGTGAAAQLPNRPVAG
KTGTSDEARDLWFIGYIPQVVTGVWLGNDNNRPTYGSSGSAAYTWHEFMEKAIEGMPIEKFPERPKLEGRKGSIKAKPIK
AKQILNRSLTPNEESAQETGSSSRRRRRSQRLEETTPTDSPRRRRRYRSQESGSSGSEESPRTRRRRPVVESDTSQPSRR
SRRSSSTVDNSSGSASSAPTPSWRERLRPNSSSSN

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=532, Percent_Identity=32.5187969924812, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI87082258, Length=277, Percent_Identity=45.8483754512635, Blast_Score=218, Evalue=1e-57,
Organism=Escherichia coli, GI1788867, Length=542, Percent_Identity=29.7047970479705, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789601, Length=170, Percent_Identity=35.8823529411765, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 83951; Mature: 83951

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRQKKLLNQISGITSGIVTRFTSREHPIYRRVWFWAGLSVGGSIIAFNYLLGEIDKTLP
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
EKAELQAVVREQTITIKAVDGTILQQQGEATREKVNLEQMPEILKNAFIASEDSRFTQHQ
HHHHHHHHHHHHEEEEEECCCHHHHHCCHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHC
GIDPQGIVRAAVNNLRSQNVVEGGSTITQQLSRILFLKQERTVWRKLKEVRLAQKLEQEL
CCCHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TKDQILERYLNLVYLGAGAYGVADAAWVYFSKPVDQLTLPEAATIAGMPPAPSLFSPDKN
HHHHHHHHHHHEEEECCCCCCCHHHHHHEECCCCCCCCCCCHHHCCCCCCCCCCCCCCCC
PQAAKQRRNLVLQRMEKEGFITPAQRQAATQAVITLKSSLPKRLQVESPYFTSYIQQELP
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHH
KYVSSEVLANGGLTVETSLNPTWQQAAEAAVAKTLRNQGRWQNFKQAALVAIDPRNGEIK
HHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEE
AMVGGQDFGQNQFNRVTQAQRQPGSTFKGFVYATAIATGKNPNDTYVDAPVVVDGYEPKN
EEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCEECCCEEEECCCCCC
YGETFRGTMNMRDALTRSVNVVALKIMLDVGFEPTIQLAKNMGIKSKLNPHYSLALGSNE
CCCHHHCCCHHHHHHHHHHHHEEEEEEHHCCCCHHHHHHHCCCCCCCCCCCEEEEECCCC
VNLLELTSAYGSFATLGLHSEPHGIRRILNRKGEVIWSAKFDSKRVLDPTSAAIMTWMLR
CEEEEEHHHHCCCEEECCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
NVVEAGTGAAAQLPNRPVAGKTGTSDEARDLWFIGYIPQVVTGVWLGNDNNRPTYGSSGS
HHHHHCCCCHHHCCCCCCCCCCCCCCCCCCEEEEECHHHHHHEEEECCCCCCCCCCCCCC
AAYTWHEFMEKAIEGMPIEKFPERPKLEGRKGSIKAKPIKAKQILNRSLTPNEESAQETG
CCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHCC
SSSRRRRRSQRLEETTPTDSPRRRRRYRSQESGSSGSEESPRTRRRRPVVESDTSQPSRR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHH
SRRSSSTVDNSSGSASSAPTPSWRERLRPNSSSSN
HHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVRQKKLLNQISGITSGIVTRFTSREHPIYRRVWFWAGLSVGGSIIAFNYLLGEIDKTLP
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
EKAELQAVVREQTITIKAVDGTILQQQGEATREKVNLEQMPEILKNAFIASEDSRFTQHQ
HHHHHHHHHHHHEEEEEECCCHHHHHCCHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHC
GIDPQGIVRAAVNNLRSQNVVEGGSTITQQLSRILFLKQERTVWRKLKEVRLAQKLEQEL
CCCHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TKDQILERYLNLVYLGAGAYGVADAAWVYFSKPVDQLTLPEAATIAGMPPAPSLFSPDKN
HHHHHHHHHHHEEEECCCCCCCHHHHHHEECCCCCCCCCCCHHHCCCCCCCCCCCCCCCC
PQAAKQRRNLVLQRMEKEGFITPAQRQAATQAVITLKSSLPKRLQVESPYFTSYIQQELP
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHH
KYVSSEVLANGGLTVETSLNPTWQQAAEAAVAKTLRNQGRWQNFKQAALVAIDPRNGEIK
HHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCEEE
AMVGGQDFGQNQFNRVTQAQRQPGSTFKGFVYATAIATGKNPNDTYVDAPVVVDGYEPKN
EEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCEECCCEEEECCCCCC
YGETFRGTMNMRDALTRSVNVVALKIMLDVGFEPTIQLAKNMGIKSKLNPHYSLALGSNE
CCCHHHCCCHHHHHHHHHHHHEEEEEEHHCCCCHHHHHHHCCCCCCCCCCCEEEEECCCC
VNLLELTSAYGSFATLGLHSEPHGIRRILNRKGEVIWSAKFDSKRVLDPTSAAIMTWMLR
CEEEEEHHHHCCCEEECCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
NVVEAGTGAAAQLPNRPVAGKTGTSDEARDLWFIGYIPQVVTGVWLGNDNNRPTYGSSGS
HHHHHCCCCHHHCCCCCCCCCCCCCCCCCCEEEEECHHHHHHEEEECCCCCCCCCCCCCC
AAYTWHEFMEKAIEGMPIEKFPERPKLEGRKGSIKAKPIKAKQILNRSLTPNEESAQETG
CCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHCC
SSSRRRRRSQRLEETTPTDSPRRRRRYRSQESGSSGSEESPRTRRRRPVVESDTSQPSRR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHH
SRRSSSTVDNSSGSASSAPTPSWRERLRPNSSSSN
HHHCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]