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The map label for this gene is hisZ [H]

Identifier: 17230407

GI number: 17230407

Start: 3555925

End: 3557139

Strand: Reverse

Name: hisZ [H]

Synonym: all2915

Alternate gene names: 17230407

Gene position: 3557139-3555925 (Counterclockwise)

Preceding gene: 17230408

Following gene: 17230406

Centisome position: 55.46

GC content: 45.35

Gene sequence:

>1215_bases
ATGGTGTATCAACCAGCAGCGGGAGCCAGGGATTTATTACCTTTAGATGTGGCTCAAAAACGCTGGATTGAAGATAAGTT
ACAACAAGTGTTTCACCGTTGGGGTTATCACAGAATCATAACCTCAACGCTGGAACGCATGGATACCTTGATGGCGGGTG
AAGCAATTCAACGTCAGATGGTGATTCAACTACAAAATTCTGAAGATGAAGAGTTGGGATTGCGTCCAGAGTTGACGGCT
TCTATTGCTCGTGCTGTTGTTACGCGCATGGCAAATCTGCGTCATCCCCAGCGCCTTTACTACAATGCCAACGTCTTTCG
TCGCATCTGGGAAAATAGGCATAATCGCCAGCAAGAGTTTTATCAAGCTGGTGTGGAATTATTGGGTGTCGGTGGACTGT
TGGCCAATGCGGAAGTGTTGCTGTTGGCGGGTAATTGTTTGACAGCATTGGGTTTACAGGATTGGCATTTAATTTTAGGT
GAAGCGGGAATCACCCGATCGCTTTTAGATGCTTTTCCCATCAATTTACGTGCTAAAGTGCGTAGTGCGATCGCTCACCT
TGACCGCATTACAATAGACACACTCCCCTTAACTGACGAACTGCGCGAACGTGCCAGAATCATGCTGGATTTGCGAGGAA
ACAGCGCCGATGTGTTGCAGAAAGTCAGTAGCCTCAATTTAGATGCAGAACAGCAAGAGGCGGTCAATAACCTCAAGTCT
CTAGTAGAGTTATTAGAATCTGGGGGTAAATTTCCGATAATTCTCGACCTGAGTCTGATTCAAACCATAGACTACTACAC
TGGTCTTGTATTTGAGATTGTCAACAATACTGATTCACAAGCAAGAGTTTTAGGGCGGGGTGGACGCTACGACCAGCTTT
TAGGGTTGTATCATCCCCAAGGAGAAAATATTCCGGGGATTGGATTTGTCCTGAATATTGAAGACTTATACCAAGTTCTT
TTATCTACACAGCAATTACCACAGGAAACCCCAGCTAGTGATTGGTTAGTCGTGCCAGAAAACCCAGCAGCCCATAGTGC
GGCTTTTGCCTACGCTCAAAAACTACGTGAATCTCCTGATTTAGTTAGAGTAGAATTAGACTTGGGTAGCACAGATAGAG
CAACCATTAAACAATATGCCCGCGATCGCAGTATTGCCCAAATCGCTTGGATCAAAGCCGACGGATCACCAATAATTGAA
AAGGTGAGTCAGTAG

Upstream 100 bases:

>100_bases
GACGCGCTACTAGCAAATTTACTACCAACCACAAGGTGCTGAAGACGAATATTTCTAGCCGCGTTTCAATCAGCGTGGGG
AATCACATAACGGAGGATGA

Downstream 100 bases:

>100_bases
TTTAGAGTCTGAAAATCTGCTGAGAGTTGACCAATGACTAAAAAGAGAGGGGAACGAGAATATGCCACACACTATTGTTA
CTGATGTTTGTGAAGGTGTT

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MVYQPAAGARDLLPLDVAQKRWIEDKLQQVFHRWGYHRIITSTLERMDTLMAGEAIQRQMVIQLQNSEDEELGLRPELTA
SIARAVVTRMANLRHPQRLYYNANVFRRIWENRHNRQQEFYQAGVELLGVGGLLANAEVLLLAGNCLTALGLQDWHLILG
EAGITRSLLDAFPINLRAKVRSAIAHLDRITIDTLPLTDELRERARIMLDLRGNSADVLQKVSSLNLDAEQQEAVNNLKS
LVELLESGGKFPIILDLSLIQTIDYYTGLVFEIVNNTDSQARVLGRGGRYDQLLGLYHPQGENIPGIGFVLNIEDLYQVL
LSTQQLPQETPASDWLVVPENPAAHSAAFAYAQKLRESPDLVRVELDLGSTDRATIKQYARDRSIAQIAWIKADGSPIIE
KVSQ

Sequences:

>Translated_404_residues
MVYQPAAGARDLLPLDVAQKRWIEDKLQQVFHRWGYHRIITSTLERMDTLMAGEAIQRQMVIQLQNSEDEELGLRPELTA
SIARAVVTRMANLRHPQRLYYNANVFRRIWENRHNRQQEFYQAGVELLGVGGLLANAEVLLLAGNCLTALGLQDWHLILG
EAGITRSLLDAFPINLRAKVRSAIAHLDRITIDTLPLTDELRERARIMLDLRGNSADVLQKVSSLNLDAEQQEAVNNLKS
LVELLESGGKFPIILDLSLIQTIDYYTGLVFEIVNNTDSQARVLGRGGRYDQLLGLYHPQGENIPGIGFVLNIEDLYQVL
LSTQQLPQETPASDWLVVPENPAAHSAAFAYAQKLRESPDLVRVELDLGSTDRATIKQYARDRSIAQIAWIKADGSPIIE
KVSQ
>Mature_404_residues
MVYQPAAGARDLLPLDVAQKRWIEDKLQQVFHRWGYHRIITSTLERMDTLMAGEAIQRQMVIQLQNSEDEELGLRPELTA
SIARAVVTRMANLRHPQRLYYNANVFRRIWENRHNRQQEFYQAGVELLGVGGLLANAEVLLLAGNCLTALGLQDWHLILG
EAGITRSLLDAFPINLRAKVRSAIAHLDRITIDTLPLTDELRERARIMLDLRGNSADVLQKVSSLNLDAEQQEAVNNLKS
LVELLESGGKFPIILDLSLIQTIDYYTGLVFEIVNNTDSQARVLGRGGRYDQLLGLYHPQGENIPGIGFVLNIEDLYQVL
LSTQQLPQETPASDWLVVPENPAAHSAAFAYAQKLRESPDLVRVELDLGSTDRATIKQYARDRSIAQIAWIKADGSPIIE
KVSQ

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine [H]

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=347, Percent_Identity=26.2247838616715, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI15029520, Length=344, Percent_Identity=25.8720930232558, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1788861, Length=415, Percent_Identity=25.7831325301205, Blast_Score=104, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71993693, Length=435, Percent_Identity=25.5172413793103, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71993686, Length=435, Percent_Identity=25.5172413793103, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6325290, Length=361, Percent_Identity=23.8227146814404, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24643061, Length=358, Percent_Identity=26.2569832402235, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24643059, Length=377, Percent_Identity=25.1989389920424, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516 [H]

Pfam domain/function: PF00587 tRNA-synt_2b [H]

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 45398; Mature: 45398

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYQPAAGARDLLPLDVAQKRWIEDKLQQVFHRWGYHRIITSTLERMDTLMAGEAIQRQM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
VIQLQNSEDEELGLRPELTASIARAVVTRMANLRHPQRLYYNANVFRRIWENRHNRQQEF
HEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHEECHHHHHHHHHHHHHHHHHH
YQAGVELLGVGGLLANAEVLLLAGNCLTALGLQDWHLILGEAGITRSLLDAFPINLRAKV
HHHHHHHHCCCHHHCCCEEEEEECCHHHHCCCCHHHHHHCCCCHHHHHHHHCCCCHHHHH
RSAIAHLDRITIDTLPLTDELRERARIMLDLRGNSADVLQKVSSLNLDAEQQEAVNNLKS
HHHHHHHHHHEEECCCCHHHHHHHHHEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHH
LVELLESGGKFPIILDLSLIQTIDYYTGLVFEIVNNTDSQARVLGRGGRYDQLLGLYHPQ
HHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHCCCCC
GENIPGIGFVLNIEDLYQVLLSTQQLPQETPASDWLVVPENPAAHSAAFAYAQKLRESPD
CCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCC
LVRVELDLGSTDRATIKQYARDRSIAQIAWIKADGSPIIEKVSQ
EEEEEECCCCCCHHHHHHHHHCCCEEEEEEEECCCCHHHHHCCC
>Mature Secondary Structure
MVYQPAAGARDLLPLDVAQKRWIEDKLQQVFHRWGYHRIITSTLERMDTLMAGEAIQRQM
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
VIQLQNSEDEELGLRPELTASIARAVVTRMANLRHPQRLYYNANVFRRIWENRHNRQQEF
HEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHEECHHHHHHHHHHHHHHHHHH
YQAGVELLGVGGLLANAEVLLLAGNCLTALGLQDWHLILGEAGITRSLLDAFPINLRAKV
HHHHHHHHCCCHHHCCCEEEEEECCHHHHCCCCHHHHHHCCCCHHHHHHHHCCCCHHHHH
RSAIAHLDRITIDTLPLTDELRERARIMLDLRGNSADVLQKVSSLNLDAEQQEAVNNLKS
HHHHHHHHHHEEECCCCHHHHHHHHHEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHH
LVELLESGGKFPIILDLSLIQTIDYYTGLVFEIVNNTDSQARVLGRGGRYDQLLGLYHPQ
HHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHCCCCC
GENIPGIGFVLNIEDLYQVLLSTQQLPQETPASDWLVVPENPAAHSAAFAYAQKLRESPD
CCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCC
LVRVELDLGSTDRATIKQYARDRSIAQIAWIKADGSPIIEKVSQ
EEEEEECCCCCCHHHHHHHHHCCCEEEEEEEECCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA