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The map label for this gene is pyk [H]

Identifier: 17230056

GI number: 17230056

Start: 3063811

End: 3065241

Strand: Reverse

Name: pyk [H]

Synonym: all2564

Alternate gene names: 17230056

Gene position: 3065241-3063811 (Counterclockwise)

Preceding gene: 17230058

Following gene: 17230055

Centisome position: 47.79

GC content: 48.36

Gene sequence:

>1431_bases
ATGCGTCGAACTAAAATTATCTGTACTGTAGGCCCGGCTACATCTGCACCAGAACGTTTAGAAGCGTTGGTAGAAGCTGG
GATGAATGTAGCACGGCTGAATTTTTCTCATGGAGCTTATGACTTCCATGCCCAAACTGCTCAGTATTTGCGGCAAATCA
GTGCTGACAGACAAAAGCCTGTGGCGATTATGCAAGACCTATGCGGGCCGAAGATTCGCTTGGGAACTTTACCACCAGAA
GGGTTAATGGTAGAGGCTGGTCAGGAAGTGACTTTTGTCTTGCAAGAAAAGGGTAGTAGTCTTGATGAATTGCCTTTACC
TTTGCCGACCTTGTTCGCAATGGTACGACCTGGGGAACCAATCTTAATTAATGATGGTCGTGTCAAGTTGATTGTGACAG
ATCGCGATGCCGATCGCATTCGGGCTATTGCCAAAATCGGGGGTCTTCTTTCTACCCGTAAGGGTGTTAACCTGCCAGCG
ACTCGTTTACCTGTCAGTTCCATCACCGAAAAGGACTTGCAGGATTTGCGTTTTGGCATCGATTTGAGTGTAGATTGGGT
GGCGGTGTCTTTTGTGCGATCGCCTTATGACCTCGAACCAGCGCAACGGATGATTGAAGCGGCGGGGAAAACTATTCGGG
TAATCGCCAAAATTGAACGTCCAGAAGCCGTTGAGCAAATTGATTCTATCATCGACGTTGCTGATGCGATTATGATTGCT
CGTGGTGACTTGGGTGTGGAAATGCCTATCCATGAAGTCCCATTGATTCAAAAGGACATCATTCGCCGTTGCAACCAAGC
TGGTAAGCCCGTGATTACAGCAACGCAAATGCTAGAGTCGATGATTAGCGCTCCCGATCCCACCCGTGCGGAGGCTACAG
ACGTTGCTAACTCTATTCTCGATGGTACAGATGCCGTCATGCTCTCTGGGGAAACGGCTGTAGGACAATATCCCGTCGCC
GCCGTCCAGGTTATGCACGATATCGCCGTGACTACAGAAAAGTCTTTGCAAGAGGGTAGTAAACATTGCTTATCCCATGA
AGCAGGTGGTCTGAGTGTCACCGAATCTGTAGCTGAAGCGGTCTGTCGTATCGCCTACGAAACAGGCGCAAAAGCGATTC
TTTGCAACACCACATCAGGGAGTACAGCAAAGCTCGTGTCGAAATATCGTCCGACTACACCCATTTTTGCGTTGACCCCC
GACGAAACCGCCTACCATCAGTTAGCACTTTCTTGGGGTGTGGAACCTTTATTAACCCCACCAGTCCACAATGCGGAAGA
AATGTTTATGAATCTCATCAACACCGCCGTGAGAACGGGTTTGGTGCATGACGGTGACAAGGTAGTTATTACTTCTGGAG
TGCCAATTGGTAAATCAGGCACAACTAGTTTAATTAAAGTGCATTCTATTGGACAACCAATCACCGCCTAA

Upstream 100 bases:

>100_bases
GGGTTAGGTTTGGCGTTAACTTTTCCACATGACGCGAAAAGTTAGAACAGTTAGTCAGTCGTAACTGTTCACTGTTCACT
ATCCCCTATCCCCAATATCT

Downstream 100 bases:

>100_bases
TATTCCTAGAATAAGTCTGAGGCTTTTGCTTCTTAGCCAAATTGGGCAATGGTTAAGAAAAGTTGCCAAAATCAGAATTG
TAAGGAATCAAAAAGGGTGT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MRRTKIICTVGPATSAPERLEALVEAGMNVARLNFSHGAYDFHAQTAQYLRQISADRQKPVAIMQDLCGPKIRLGTLPPE
GLMVEAGQEVTFVLQEKGSSLDELPLPLPTLFAMVRPGEPILINDGRVKLIVTDRDADRIRAIAKIGGLLSTRKGVNLPA
TRLPVSSITEKDLQDLRFGIDLSVDWVAVSFVRSPYDLEPAQRMIEAAGKTIRVIAKIERPEAVEQIDSIIDVADAIMIA
RGDLGVEMPIHEVPLIQKDIIRRCNQAGKPVITATQMLESMISAPDPTRAEATDVANSILDGTDAVMLSGETAVGQYPVA
AVQVMHDIAVTTEKSLQEGSKHCLSHEAGGLSVTESVAEAVCRIAYETGAKAILCNTTSGSTAKLVSKYRPTTPIFALTP
DETAYHQLALSWGVEPLLTPPVHNAEEMFMNLINTAVRTGLVHDGDKVVITSGVPIGKSGTTSLIKVHSIGQPITA

Sequences:

>Translated_476_residues
MRRTKIICTVGPATSAPERLEALVEAGMNVARLNFSHGAYDFHAQTAQYLRQISADRQKPVAIMQDLCGPKIRLGTLPPE
GLMVEAGQEVTFVLQEKGSSLDELPLPLPTLFAMVRPGEPILINDGRVKLIVTDRDADRIRAIAKIGGLLSTRKGVNLPA
TRLPVSSITEKDLQDLRFGIDLSVDWVAVSFVRSPYDLEPAQRMIEAAGKTIRVIAKIERPEAVEQIDSIIDVADAIMIA
RGDLGVEMPIHEVPLIQKDIIRRCNQAGKPVITATQMLESMISAPDPTRAEATDVANSILDGTDAVMLSGETAVGQYPVA
AVQVMHDIAVTTEKSLQEGSKHCLSHEAGGLSVTESVAEAVCRIAYETGAKAILCNTTSGSTAKLVSKYRPTTPIFALTP
DETAYHQLALSWGVEPLLTPPVHNAEEMFMNLINTAVRTGLVHDGDKVVITSGVPIGKSGTTSLIKVHSIGQPITA
>Mature_476_residues
MRRTKIICTVGPATSAPERLEALVEAGMNVARLNFSHGAYDFHAQTAQYLRQISADRQKPVAIMQDLCGPKIRLGTLPPE
GLMVEAGQEVTFVLQEKGSSLDELPLPLPTLFAMVRPGEPILINDGRVKLIVTDRDADRIRAIAKIGGLLSTRKGVNLPA
TRLPVSSITEKDLQDLRFGIDLSVDWVAVSFVRSPYDLEPAQRMIEAAGKTIRVIAKIERPEAVEQIDSIIDVADAIMIA
RGDLGVEMPIHEVPLIQKDIIRRCNQAGKPVITATQMLESMISAPDPTRAEATDVANSILDGTDAVMLSGETAVGQYPVA
AVQVMHDIAVTTEKSLQEGSKHCLSHEAGGLSVTESVAEAVCRIAYETGAKAILCNTTSGSTAKLVSKYRPTTPIFALTP
DETAYHQLALSWGVEPLLTPPVHNAEEMFMNLINTAVRTGLVHDGDKVVITSGVPIGKSGTTSLIKVHSIGQPITA

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=489, Percent_Identity=39.2638036809816, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI33286420, Length=489, Percent_Identity=39.2638036809816, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=39.0593047034765, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI32967597, Length=490, Percent_Identity=40, Blast_Score=322, Evalue=7e-88,
Organism=Homo sapiens, GI10835121, Length=490, Percent_Identity=40, Blast_Score=321, Evalue=8e-88,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=40.0696864111498, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=40.0696864111498, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=35.8649789029536, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=35.8649789029536, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=34.5794392523364, Blast_Score=135, Evalue=1e-31,
Organism=Escherichia coli, GI1787965, Length=478, Percent_Identity=42.0502092050209, Blast_Score=341, Evalue=5e-95,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=39.375, Blast_Score=295, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI17544584, Length=476, Percent_Identity=39.4957983193277, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17506829, Length=481, Percent_Identity=38.4615384615385, Blast_Score=291, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI71984413, Length=481, Percent_Identity=38.4615384615385, Blast_Score=291, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI71984406, Length=481, Percent_Identity=38.4615384615385, Blast_Score=291, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI17506831, Length=481, Percent_Identity=38.4615384615385, Blast_Score=291, Evalue=7e-79,
Organism=Saccharomyces cerevisiae, GI6319279, Length=487, Percent_Identity=37.782340862423, Blast_Score=290, Evalue=3e-79,
Organism=Saccharomyces cerevisiae, GI6324923, Length=488, Percent_Identity=37.7049180327869, Blast_Score=288, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24648964, Length=490, Percent_Identity=38.3673469387755, Blast_Score=319, Evalue=2e-87,
Organism=Drosophila melanogaster, GI28571814, Length=490, Percent_Identity=38.3673469387755, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI24648966, Length=429, Percent_Identity=39.8601398601399, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24581235, Length=487, Percent_Identity=31.006160164271, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24646914, Length=313, Percent_Identity=38.3386581469649, Blast_Score=182, Evalue=3e-46,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51150; Mature: 51150

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTKIICTVGPATSAPERLEALVEAGMNVARLNFSHGAYDFHAQTAQYLRQISADRQKP
CCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCC
VAIMQDLCGPKIRLGTLPPEGLMVEAGQEVTFVLQEKGSSLDELPLPLPTLFAMVRPGEP
HHHHHHCCCCCEEECCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCE
ILINDGRVKLIVTDRDADRIRAIAKIGGLLSTRKGVNLPATRLPVSSITEKDLQDLRFGI
EEEECCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHHHHHHHHHHCC
DLSVDWVAVSFVRSPYDLEPAQRMIEAAGKTIRVIAKIERPEAVEQIDSIIDVADAIMIA
CCEEHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHEE
RGDLGVEMPIHEVPLIQKDIIRRCNQAGKPVITATQMLESMISAPDPTRAEATDVANSIL
CCCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHH
DGTDAVMLSGETAVGQYPVAAVQVMHDIAVTTEKSLQEGSKHCLSHEAGGLSVTESVAEA
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
VCRIAYETGAKAILCNTTSGSTAKLVSKYRPTTPIFALTPDETAYHQLALSWGVEPLLTP
HHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCC
PVHNAEEMFMNLINTAVRTGLVHDGDKVVITSGVPIGKSGTTSLIKVHSIGQPITA
CCCCHHHHHHHHHHHHHHHCCEECCCEEEEECCCCCCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MRRTKIICTVGPATSAPERLEALVEAGMNVARLNFSHGAYDFHAQTAQYLRQISADRQKP
CCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCC
VAIMQDLCGPKIRLGTLPPEGLMVEAGQEVTFVLQEKGSSLDELPLPLPTLFAMVRPGEP
HHHHHHCCCCCEEECCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHCCCCCE
ILINDGRVKLIVTDRDADRIRAIAKIGGLLSTRKGVNLPATRLPVSSITEKDLQDLRFGI
EEEECCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHHHHHHHHHHCC
DLSVDWVAVSFVRSPYDLEPAQRMIEAAGKTIRVIAKIERPEAVEQIDSIIDVADAIMIA
CCEEHHHHHHHHCCCCCCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHEE
RGDLGVEMPIHEVPLIQKDIIRRCNQAGKPVITATQMLESMISAPDPTRAEATDVANSIL
CCCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHH
DGTDAVMLSGETAVGQYPVAAVQVMHDIAVTTEKSLQEGSKHCLSHEAGGLSVTESVAEA
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
VCRIAYETGAKAILCNTTSGSTAKLVSKYRPTTPIFALTPDETAYHQLALSWGVEPLLTP
HHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCCCCCC
PVHNAEEMFMNLINTAVRTGLVHDGDKVVITSGVPIGKSGTTSLIKVHSIGQPITA
CCCCHHHHHHHHHHHHHHHCCEECCCEEEEECCCCCCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436141; 1447787 [H]