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The map label for this gene is lepA [H]

Identifier: 17230000

GI number: 17230000

Start: 3010134

End: 3011945

Strand: Reverse

Name: lepA [H]

Synonym: all2508

Alternate gene names: 17230000

Gene position: 3011945-3010134 (Counterclockwise)

Preceding gene: 17230001

Following gene: 17229999

Centisome position: 46.96

GC content: 46.41

Gene sequence:

>1812_bases
ATGACTGACGTTCCCGCAGTTCGCATTCGCAATTTTTGTATTATTGCTCACATCGATCATGGGAAATCTACCCTGGCCGA
TCGCTTACTGCAAGCCACTGGCACTGTTGACGAGCGGCAAATGAAGGAACAGTTTCTCGACAACATGGATTTGGAACGGG
AGCGCGGCATTACAATTAAGCTGCAAGCTGCCCGGATGAATTATCAAGCCAAAGATGGGCAGCAGTATGTACTGAATTTA
ATTGATACGCCTGGCCATGTGGACTTTTCCTATGAAGTTTCCCGCAGTTTGGCAGCGTGTGAGGGAGCTTTGTTAGTGGT
AGACGCATCTCAAGGGGTTGAGGCGCAAACTCTGGCAAACGTATATTTAGCTCTCGAACATAATCTAGAAATTATCCCAG
TTCTGAACAAAATCGACTTACCAGGGGCAGAACCAGACCGGGTAGTCGGCGAAATTGAAGAAATTATTGGTCTAGATTGC
AGTGGGGCAATTCTCGCTTCGGCTAAAGAAGGAATCGGTATTAATGAAATTCTCGAAGCCGTTGTTGAGCGTGTACCACC
ACCACCCAATACAGTTGATCAGCGCTTACGAGCATTAATCTTCGATAGTTACTATGACATATATCGCGGTGTCATTGTAT
ACTTCCGGGTAATGGATGGAACAGTGAAAAAAGGCGATCGCGTCTATCTCATGGCTTCCGAGAAAGAATACGAAATCGAT
GAATTAGGCGTTCTTTCTCCCACCCAAAAACCAGTCAATGAACTGCACGCTGGGGAGGTGGGTTATTTCGGTGCAGCAAT
TAAAGCAGTAGCTGATGCGCGAGTGGGTGACACTATCACCTTATGCAACGCCAAAGCAACAGAAGCTTTACCGGGATACA
CAGAAGCCAACCCAATGGTTTTTTGTGGAATGTTCCCCATCGATGCTGACCAATTTGAAGATTTGCGGGAAGCCTTAGAG
AAGTTGAGGCTCAATGATGCTGCGCTGCAATATGAACCAGAAACCTCTAGCGCGATGGGTTTTGGGTTCCGTTGTGGGTT
CTTGGGTTTGCTGCACATGGAAATCGTCCAGGAACGCTTGGAAAGAGAGTATGACCTAGATTTAATTATTACAGCCCCCT
CGGTGGTGTATAAAGTCATCACCACCAAAGGCGAAGAACTGTATATAGATAATCCTAGCCATTTACCAGCCCCCAACGAT
CGCGAAAGGATTGAGGAACCATACGTGAAGGTGGAAATGATTACACCGGAAACCTATGTCGGCACTTTGATGGAGTTGTC
GCAAAATCGCCGGGGTATCTTCAAAGATATGAAATATCTCACCCAAGGACGTACCACCCTCACCTACGAAATTCCTTTGG
CAGAAGTTGTTACCGACTTTTTCGACCAAATGAAATCGCGATCGCGTGGTTATGCCAGTATGGAATATCACCTCATCGGC
TACCGCGAGAACCCCTTGGTGAAGTTGGACATCATGATTAATGGTGATCCTGTGGATTCCTTGGCGATGATTGTTCACCG
AGATAAAGCCTATGGTATGGGACGTTCAATGGCGGAAAAACTCAAGGAATTAATTCCCCGCCATCAATTTAAAGTCCCCA
TTCAAGCGTCTATTGGTAGCAAAGTCATCGCCAGCGAACATATTCCCGCCTTGCGTAAAGATGTACTCGCCAAGTGTTAT
GGCGGTGACATCAGCCGGAAGAAGAAACTCTTGCAGAAACAAGCAAAAGGTAAAAAGCGGATGAAATCCGTCGGTACAGT
CGATGTACCCCAGGAAGCTTTTATGGCTGTATTGCGTTTAGATCAAAGTTAG

Upstream 100 bases:

>100_bases
ACCTTTTGCCTTCTTAATGCGCGTTGGCTAATAAAGCATTAAAATACATGAAGTAAAATGCTCTTTTAACCTTGGTTGCT
GCTTCAGCACAGATTAGTAT

Downstream 100 bases:

>100_bases
AGATAATAGCTTAGTGTTCCTAGCTTGGCAATCTTTCCAGCTAACTAGGCTTGACATCTCATCTTGCGTATCTGCATTAG
GTGAACCATCGGAAAATTAT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MTDVPAVRIRNFCIIAHIDHGKSTLADRLLQATGTVDERQMKEQFLDNMDLERERGITIKLQAARMNYQAKDGQQYVLNL
IDTPGHVDFSYEVSRSLAACEGALLVVDASQGVEAQTLANVYLALEHNLEIIPVLNKIDLPGAEPDRVVGEIEEIIGLDC
SGAILASAKEGIGINEILEAVVERVPPPPNTVDQRLRALIFDSYYDIYRGVIVYFRVMDGTVKKGDRVYLMASEKEYEID
ELGVLSPTQKPVNELHAGEVGYFGAAIKAVADARVGDTITLCNAKATEALPGYTEANPMVFCGMFPIDADQFEDLREALE
KLRLNDAALQYEPETSSAMGFGFRCGFLGLLHMEIVQERLEREYDLDLIITAPSVVYKVITTKGEELYIDNPSHLPAPND
RERIEEPYVKVEMITPETYVGTLMELSQNRRGIFKDMKYLTQGRTTLTYEIPLAEVVTDFFDQMKSRSRGYASMEYHLIG
YRENPLVKLDIMINGDPVDSLAMIVHRDKAYGMGRSMAEKLKELIPRHQFKVPIQASIGSKVIASEHIPALRKDVLAKCY
GGDISRKKKLLQKQAKGKKRMKSVGTVDVPQEAFMAVLRLDQS

Sequences:

>Translated_603_residues
MTDVPAVRIRNFCIIAHIDHGKSTLADRLLQATGTVDERQMKEQFLDNMDLERERGITIKLQAARMNYQAKDGQQYVLNL
IDTPGHVDFSYEVSRSLAACEGALLVVDASQGVEAQTLANVYLALEHNLEIIPVLNKIDLPGAEPDRVVGEIEEIIGLDC
SGAILASAKEGIGINEILEAVVERVPPPPNTVDQRLRALIFDSYYDIYRGVIVYFRVMDGTVKKGDRVYLMASEKEYEID
ELGVLSPTQKPVNELHAGEVGYFGAAIKAVADARVGDTITLCNAKATEALPGYTEANPMVFCGMFPIDADQFEDLREALE
KLRLNDAALQYEPETSSAMGFGFRCGFLGLLHMEIVQERLEREYDLDLIITAPSVVYKVITTKGEELYIDNPSHLPAPND
RERIEEPYVKVEMITPETYVGTLMELSQNRRGIFKDMKYLTQGRTTLTYEIPLAEVVTDFFDQMKSRSRGYASMEYHLIG
YRENPLVKLDIMINGDPVDSLAMIVHRDKAYGMGRSMAEKLKELIPRHQFKVPIQASIGSKVIASEHIPALRKDVLAKCY
GGDISRKKKLLQKQAKGKKRMKSVGTVDVPQEAFMAVLRLDQS
>Mature_602_residues
TDVPAVRIRNFCIIAHIDHGKSTLADRLLQATGTVDERQMKEQFLDNMDLERERGITIKLQAARMNYQAKDGQQYVLNLI
DTPGHVDFSYEVSRSLAACEGALLVVDASQGVEAQTLANVYLALEHNLEIIPVLNKIDLPGAEPDRVVGEIEEIIGLDCS
GAILASAKEGIGINEILEAVVERVPPPPNTVDQRLRALIFDSYYDIYRGVIVYFRVMDGTVKKGDRVYLMASEKEYEIDE
LGVLSPTQKPVNELHAGEVGYFGAAIKAVADARVGDTITLCNAKATEALPGYTEANPMVFCGMFPIDADQFEDLREALEK
LRLNDAALQYEPETSSAMGFGFRCGFLGLLHMEIVQERLEREYDLDLIITAPSVVYKVITTKGEELYIDNPSHLPAPNDR
ERIEEPYVKVEMITPETYVGTLMELSQNRRGIFKDMKYLTQGRTTLTYEIPLAEVVTDFFDQMKSRSRGYASMEYHLIGY
RENPLVKLDIMINGDPVDSLAMIVHRDKAYGMGRSMAEKLKELIPRHQFKVPIQASIGSKVIASEHIPALRKDVLAKCYG
GDISRKKKLLQKQAKGKKRMKSVGTVDVPQEAFMAVLRLDQS

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=611, Percent_Identity=48.772504091653, Blast_Score=636, Evalue=0.0,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=46.2121212121212, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI25306283, Length=153, Percent_Identity=45.7516339869281, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI19923640, Length=153, Percent_Identity=45.7516339869281, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI25306287, Length=153, Percent_Identity=45.7516339869281, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI4503483, Length=143, Percent_Identity=41.958041958042, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI18390331, Length=155, Percent_Identity=38.0645161290323, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=44.0366972477064, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=44.0366972477064, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=44.0366972477064, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=33.0827067669173, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=33.0827067669173, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI5729864, Length=270, Percent_Identity=24.4444444444444, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI223555963, Length=270, Percent_Identity=24.4444444444444, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI94966752, Length=131, Percent_Identity=32.0610687022901, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI53729339, Length=247, Percent_Identity=27.1255060728745, Blast_Score=66, Evalue=1e-10,
Organism=Homo sapiens, GI53729337, Length=247, Percent_Identity=27.1255060728745, Blast_Score=66, Evalue=1e-10,
Organism=Escherichia coli, GI1788922, Length=592, Percent_Identity=57.2635135135135, Blast_Score=695, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=473, Percent_Identity=32.1353065539112, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1789738, Length=187, Percent_Identity=36.8983957219251, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1790835, Length=159, Percent_Identity=32.7044025157233, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789559, Length=254, Percent_Identity=27.9527559055118, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI2367247, Length=249, Percent_Identity=28.1124497991968, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=41.8300653594771, Blast_Score=488, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17556745, Length=473, Percent_Identity=25.7928118393235, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17533571, Length=147, Percent_Identity=41.4965986394558, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71988819, Length=136, Percent_Identity=38.9705882352941, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71988811, Length=136, Percent_Identity=38.9705882352941, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=155, Percent_Identity=36.7741935483871, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=141, Percent_Identity=34.7517730496454, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=247, Percent_Identity=28.7449392712551, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=604, Percent_Identity=44.0397350993377, Blast_Score=523, Evalue=1e-149,
Organism=Saccharomyces cerevisiae, GI6323098, Length=151, Percent_Identity=40.3973509933775, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=143, Percent_Identity=42.6573426573427, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=143, Percent_Identity=42.6573426573427, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6324166, Length=141, Percent_Identity=41.1347517730496, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322359, Length=151, Percent_Identity=36.4238410596026, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6322675, Length=147, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=244, Percent_Identity=30.327868852459, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI78706572, Length=604, Percent_Identity=46.6887417218543, Blast_Score=550, Evalue=1e-156,
Organism=Drosophila melanogaster, GI28574573, Length=227, Percent_Identity=36.1233480176211, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24582462, Length=156, Percent_Identity=41.025641025641, Blast_Score=108, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585711, Length=147, Percent_Identity=39.4557823129252, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24585713, Length=147, Percent_Identity=39.4557823129252, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24585709, Length=147, Percent_Identity=39.4557823129252, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI21357743, Length=143, Percent_Identity=34.965034965035, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI221458488, Length=150, Percent_Identity=38, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45550900, Length=275, Percent_Identity=28.3636363636364, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI281363316, Length=301, Percent_Identity=28.2392026578073, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17864358, Length=301, Percent_Identity=28.2392026578073, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28572034, Length=224, Percent_Identity=29.9107142857143, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19921738, Length=308, Percent_Identity=26.2987012987013, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67450; Mature: 67318

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVPAVRIRNFCIIAHIDHGKSTLADRLLQATGTVDERQMKEQFLDNMDLERERGITIK
CCCCCCEEECCEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHCCCEEE
LQAARMNYQAKDGQQYVLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQGVEAQTLAN
EEEEECCCCCCCCHHHHHHEECCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHH
VYLALEHNLEIIPVLNKIDLPGAEPDRVVGEIEEIIGLDCSGAILASAKEGIGINEILEA
HHEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHH
VVERVPPPPNTVDQRLRALIFDSYYDIYRGVIVYFRVMDGTVKKGDRVYLMASEKEYEID
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHH
ELGVLSPTQKPVNELHAGEVGYFGAAIKAVADARVGDTITLCNAKATEALPGYTEANPMV
HCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCCCCCCCEE
FCGMFPIDADQFEDLREALEKLRLNDAALQYEPETSSAMGFGFRCGFLGLLHMEIVQERL
EEEECCCCHHHHHHHHHHHHHHCCCCHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EREYDLDLIITAPSVVYKVITTKGEELYIDNPSHLPAPNDRERIEEPYVKVEMITPETYV
HHHCCCEEEEECHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHCCCCEEEEEECCHHHH
GTLMELSQNRRGIFKDMKYLTQGRTTLTYEIPLAEVVTDFFDQMKSRSRGYASMEYHLIG
HHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEEEE
YRENPLVKLDIMINGDPVDSLAMIVHRDKAYGMGRSMAEKLKELIPRHQFKVPIQASIGS
ECCCCEEEEEEEECCCCHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCCCCCCEEECCCCC
KVIASEHIPALRKDVLAKCYGGDISRKKKLLQKQAKGKKRMKSVGTVDVPQEAFMAVLRL
HHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
DQS
CCC
>Mature Secondary Structure 
TDVPAVRIRNFCIIAHIDHGKSTLADRLLQATGTVDERQMKEQFLDNMDLERERGITIK
CCCCCEEECCEEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHCCCEEE
LQAARMNYQAKDGQQYVLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQGVEAQTLAN
EEEEECCCCCCCCHHHHHHEECCCCCCEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHH
VYLALEHNLEIIPVLNKIDLPGAEPDRVVGEIEEIIGLDCSGAILASAKEGIGINEILEA
HHEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHH
VVERVPPPPNTVDQRLRALIFDSYYDIYRGVIVYFRVMDGTVKKGDRVYLMASEKEYEID
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHH
ELGVLSPTQKPVNELHAGEVGYFGAAIKAVADARVGDTITLCNAKATEALPGYTEANPMV
HCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCCCCCCCEE
FCGMFPIDADQFEDLREALEKLRLNDAALQYEPETSSAMGFGFRCGFLGLLHMEIVQERL
EEEECCCCHHHHHHHHHHHHHHCCCCHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EREYDLDLIITAPSVVYKVITTKGEELYIDNPSHLPAPNDRERIEEPYVKVEMITPETYV
HHHCCCEEEEECHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHCCCCEEEEEECCHHHH
GTLMELSQNRRGIFKDMKYLTQGRTTLTYEIPLAEVVTDFFDQMKSRSRGYASMEYHLIG
HHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEEEE
YRENPLVKLDIMINGDPVDSLAMIVHRDKAYGMGRSMAEKLKELIPRHQFKVPIQASIGS
ECCCCEEEEEEEECCCCHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCCCCCCEEECCCCC
KVIASEHIPALRKDVLAKCYGGDISRKKKLLQKQAKGKKRMKSVGTVDVPQEAFMAVLRL
HHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
DQS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA