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The map label for this gene is grpE [H]

Identifier: 17229937

GI number: 17229937

Start: 2939744

End: 2940490

Strand: Direct

Name: grpE [H]

Synonym: alr2445

Alternate gene names: 17229937

Gene position: 2939744-2940490 (Clockwise)

Preceding gene: 17229932

Following gene: 17229938

Centisome position: 45.83

GC content: 44.18

Gene sequence:

>747_bases
ATGATCGACGAAAATAAACAGGTAAACCATACAAGCCAGCAATTGGGTGAATCAACAGAGGTAAAGCAAGCAATTATGAG
TGAATCCCCAGCCCAAATTAACAATAACGAATCTGCTAACGAAGTAACTGAGCCAGTGGCAATCCCAACCAATGTAGTGG
GAGACACTACAGCTACAGAAGATAATGGCTTCACAGCAACACAGATACAGGAAGCGAATACAGCTGCTTTGGCTGAATTG
ACTCAACAGATCAGTTCTCTCAAAACGCAGTTAGATGAACGCAGTACGCAATATATGCGGATCGCGGCAGATTTTGAAAA
TTACCGCAAGCGCACTCAAAAAGAAAAAGAAGAGTTGGACTTACAGGTAAAACGAAACACAATTTTGGAGTTGCTACCTA
TTGTCGATAATTTTGAGCGGGCGCGATCGCATCTTAAGCCACAAACTGAAAGTGAGATGACAATTCACAAAAGTTATCAA
GGAGTATACAAACAACTCGTAGATTCCCTGAAGCGCCTGGGTGTATCACCAATGCGTCCCGAAGGACAAGAGTTTGATCC
CAACCTTCATGAAGCAGTAATGCGGGAACCTACTGATGAACATCCTGAAGGAACAGTGTTAGAAGAGTTAGTACGTGGAT
ATTACTTGGGCGATCGCGTGCTACGCCATTCTATGGTCAAGGTGGCTGCTCCAAAGGAAGATACGCTGCCTGCACAAGAA
AATCAGTCGAGTCCAGCCGACAGTTAA

Upstream 100 bases:

>100_bases
GCAAAAAAATCAGTCTCAGTGTACAGCCATCAATTGTCAGGGTTGTTCATTGTGATGAAATACTGCCGTAAATCCTATTT
GGGGTGAGTAGACTTGGACA

Downstream 100 bases:

>100_bases
GCTGTATCAGCTCGCCTCGCTGACCAATAGTGCTTAGGTCATAGAAGAGGCGAGCATTGTTAGGGATTGGGGACTGGGGA
CTGGAGATTGGGAACTGGGG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MIDENKQVNHTSQQLGESTEVKQAIMSESPAQINNNESANEVTEPVAIPTNVVGDTTATEDNGFTATQIQEANTAALAEL
TQQISSLKTQLDERSTQYMRIAADFENYRKRTQKEKEELDLQVKRNTILELLPIVDNFERARSHLKPQTESEMTIHKSYQ
GVYKQLVDSLKRLGVSPMRPEGQEFDPNLHEAVMREPTDEHPEGTVLEELVRGYYLGDRVLRHSMVKVAAPKEDTLPAQE
NQSSPADS

Sequences:

>Translated_248_residues
MIDENKQVNHTSQQLGESTEVKQAIMSESPAQINNNESANEVTEPVAIPTNVVGDTTATEDNGFTATQIQEANTAALAEL
TQQISSLKTQLDERSTQYMRIAADFENYRKRTQKEKEELDLQVKRNTILELLPIVDNFERARSHLKPQTESEMTIHKSYQ
GVYKQLVDSLKRLGVSPMRPEGQEFDPNLHEAVMREPTDEHPEGTVLEELVRGYYLGDRVLRHSMVKVAAPKEDTLPAQE
NQSSPADS
>Mature_248_residues
MIDENKQVNHTSQQLGESTEVKQAIMSESPAQINNNESANEVTEPVAIPTNVVGDTTATEDNGFTATQIQEANTAALAEL
TQQISSLKTQLDERSTQYMRIAADFENYRKRTQKEKEELDLQVKRNTILELLPIVDNFERARSHLKPQTESEMTIHKSYQ
GVYKQLVDSLKRLGVSPMRPEGQEFDPNLHEAVMREPTDEHPEGTVLEELVRGYYLGDRVLRHSMVKVAAPKEDTLPAQE
NQSSPADS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=155, Percent_Identity=28.3870967741935, Blast_Score=73, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324806, Length=167, Percent_Identity=29.3413173652695, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 27934; Mature: 27934

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDENKQVNHTSQQLGESTEVKQAIMSESPAQINNNESANEVTEPVAIPTNVVGDTTATE
CCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCC
DNGFTATQIQEANTAALAELTQQISSLKTQLDERSTQYMRIAADFENYRKRTQKEKEELD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQVKRNTILELLPIVDNFERARSHLKPQTESEMTIHKSYQGVYKQLVDSLKRLGVSPMRP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EGQEFDPNLHEAVMREPTDEHPEGTVLEELVRGYYLGDRVLRHSMVKVAAPKEDTLPAQE
CCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
NQSSPADS
CCCCCCCC
>Mature Secondary Structure
MIDENKQVNHTSQQLGESTEVKQAIMSESPAQINNNESANEVTEPVAIPTNVVGDTTATE
CCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCC
DNGFTATQIQEANTAALAELTQQISSLKTQLDERSTQYMRIAADFENYRKRTQKEKEELD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQVKRNTILELLPIVDNFERARSHLKPQTESEMTIHKSYQGVYKQLVDSLKRLGVSPMRP
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EGQEFDPNLHEAVMREPTDEHPEGTVLEELVRGYYLGDRVLRHSMVKVAAPKEDTLPAQE
CCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
NQSSPADS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA