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The map label for this gene is amyS [H]

Identifier: 17229682

GI number: 17229682

Start: 2632724

End: 2634202

Strand: Direct

Name: amyS [H]

Synonym: alr2190

Alternate gene names: 17229682

Gene position: 2632724-2634202 (Clockwise)

Preceding gene: 17229681

Following gene: 17229683

Centisome position: 41.05

GC content: 41.51

Gene sequence:

>1479_bases
ATGGCACAGATGAATGGCACAATGATGCAATACTTCCACTGGTACATCCCTAACGATGGGAACTTGTGGAGTAAAGTTGA
GGCTTCAGCACCAGAATTAGCAGATGCAGGTTTTACAGCCATGTGGCTACCACCAGCCTACAAGGGCTTTGCTGGGTCGT
TTGATGTGGGATATGGTGTTTACGACTTATTTGATTTAGGTGAGTTTGATCAAAAAGGCTCGGTGCGGACGAAATATGGT
ACACGTCAGCAGTATCTTGATGCGGTTAAATCTCTGCAAACCCATGGTCTACAAGTTTATGCTGATGCGGTACTAAATCA
TAAGATGGGTGGTGATGCAGTAGAAACACCAAAAGCCACTCCTTTTCCTCAAGACGATCGCCTGAATCCTAAAGGTGGGT
TACAAGATATTAAGACCTACACTCATTATAATTTCCCTGGACGGCAAGGCAAATATTCTAACTTTGAGTGGCATTGGTGG
CATTTTGATGCTGTTGATTATAACGAATATAACAGTGGCGATCGCAGCACAGTTTACTTATTAGAAGGTAAAAACTTTGA
CGACTACGTAGCCTTAGAAAAAGGCAACTTTGCCTATTTGATGGGTTGTGATCTGGATTTTCAAAATGAGTGGGTGCGCG
GTGAAGTTACTTACTGGGGTAAGTGGTGTCTTGACACTACAAAAGTAGATGGTTTCCGCATCGATGCTATTAAACATATT
TCCACATGGTTCTTTCCTGAATGGATAGATGCACTAGAACGCCATGCTGGTAAAGATTTATTTATGGTAGGAGAGTATTG
GTATAACGATATTAATACTCTGCTTTGGTATGTTGATGCTGTTCGTGGCAAAATGTCAGTTTTTGACGTACCACTACATT
ACAACTTCCATCAAGCCAGCAAATCTGGAGGCAATTATGATATGCGCCGGATTTTGGATGGCACAATGATGCAACAACGT
CCTACCCATGCTGTGACATTTGTCGAAAATCATGATTCTCAGCCTTTGCAAGCTTTGGAATCTGTCGTTGAACCTTGGTT
TAAACCCTTAGCTTACGCCATTATTTTGTTACGACAAGAAGGCTATCCTTGTGTCTTTCATGCTGACTACTACGGTGCAG
AGTATGAAGACTGGGGGAAAGATGGCAATCGTTACAATATTTTTATGCCGTCCCACCGTTGGATAATTGATAAGTTACTT
TATGCACGTAAACATTATGCTTATGGGCCACAATACAATTATTTAGACCATTGGAATACTATTGGTTGGACACGCTTAGG
TGATGCAGATCATCCTCAAGGAATGGCTGTGATTATGAGTGATGGTTCAGAAGGTATTAAATGGATGGAAGTTGGTAAAC
CCAATACTAAGTTTATTGACTTAACTGAACATATCAAAGAAGCTGTATATACCAATGAATGGGGCTGGGGTGAATTTCGT
TGTCTAGGCGGTTCAGTATCTGTTTGGGTGCAAGCGTAA

Upstream 100 bases:

>100_bases
CTTCGAGAGTTTCAAAATTAAATCGCATGAAAAATTCCGAATTACCACCAATCTCGACTGTATCTCTCGTCAAAGGTGAT
ATTTACAGACACAAAGAGAC

Downstream 100 bases:

>100_bases
AATATTTAGAACCCCAACCTCGCAATGCTGCTATGCGGTCGCACCCATTTTAAACACTTGTGGTAAATCCACTATTGCCT
CATCTTCTCTAAAGATTAAC

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MAQMNGTMMQYFHWYIPNDGNLWSKVEASAPELADAGFTAMWLPPAYKGFAGSFDVGYGVYDLFDLGEFDQKGSVRTKYG
TRQQYLDAVKSLQTHGLQVYADAVLNHKMGGDAVETPKATPFPQDDRLNPKGGLQDIKTYTHYNFPGRQGKYSNFEWHWW
HFDAVDYNEYNSGDRSTVYLLEGKNFDDYVALEKGNFAYLMGCDLDFQNEWVRGEVTYWGKWCLDTTKVDGFRIDAIKHI
STWFFPEWIDALERHAGKDLFMVGEYWYNDINTLLWYVDAVRGKMSVFDVPLHYNFHQASKSGGNYDMRRILDGTMMQQR
PTHAVTFVENHDSQPLQALESVVEPWFKPLAYAIILLRQEGYPCVFHADYYGAEYEDWGKDGNRYNIFMPSHRWIIDKLL
YARKHYAYGPQYNYLDHWNTIGWTRLGDADHPQGMAVIMSDGSEGIKWMEVGKPNTKFIDLTEHIKEAVYTNEWGWGEFR
CLGGSVSVWVQA

Sequences:

>Translated_492_residues
MAQMNGTMMQYFHWYIPNDGNLWSKVEASAPELADAGFTAMWLPPAYKGFAGSFDVGYGVYDLFDLGEFDQKGSVRTKYG
TRQQYLDAVKSLQTHGLQVYADAVLNHKMGGDAVETPKATPFPQDDRLNPKGGLQDIKTYTHYNFPGRQGKYSNFEWHWW
HFDAVDYNEYNSGDRSTVYLLEGKNFDDYVALEKGNFAYLMGCDLDFQNEWVRGEVTYWGKWCLDTTKVDGFRIDAIKHI
STWFFPEWIDALERHAGKDLFMVGEYWYNDINTLLWYVDAVRGKMSVFDVPLHYNFHQASKSGGNYDMRRILDGTMMQQR
PTHAVTFVENHDSQPLQALESVVEPWFKPLAYAIILLRQEGYPCVFHADYYGAEYEDWGKDGNRYNIFMPSHRWIIDKLL
YARKHYAYGPQYNYLDHWNTIGWTRLGDADHPQGMAVIMSDGSEGIKWMEVGKPNTKFIDLTEHIKEAVYTNEWGWGEFR
CLGGSVSVWVQA
>Mature_491_residues
AQMNGTMMQYFHWYIPNDGNLWSKVEASAPELADAGFTAMWLPPAYKGFAGSFDVGYGVYDLFDLGEFDQKGSVRTKYGT
RQQYLDAVKSLQTHGLQVYADAVLNHKMGGDAVETPKATPFPQDDRLNPKGGLQDIKTYTHYNFPGRQGKYSNFEWHWWH
FDAVDYNEYNSGDRSTVYLLEGKNFDDYVALEKGNFAYLMGCDLDFQNEWVRGEVTYWGKWCLDTTKVDGFRIDAIKHIS
TWFFPEWIDALERHAGKDLFMVGEYWYNDINTLLWYVDAVRGKMSVFDVPLHYNFHQASKSGGNYDMRRILDGTMMQQRP
THAVTFVENHDSQPLQALESVVEPWFKPLAYAIILLRQEGYPCVFHADYYGAEYEDWGKDGNRYNIFMPSHRWIIDKLLY
ARKHYAYGPQYNYLDHWNTIGWTRLGDADHPQGMAVIMSDGSEGIKWMEVGKPNTKFIDLTEHIKEAVYTNEWGWGEFRC
LGGSVSVWVQA

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=490, Percent_Identity=46.3265306122449, Blast_Score=441, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR015237
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF09154 DUF1939 [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 57063; Mature: 56932

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQMNGTMMQYFHWYIPNDGNLWSKVEASAPELADAGFTAMWLPPAYKGFAGSFDVGYGV
CCCCCCCEEEEEEEEECCCCCEEEECCCCCCCHHCCCCEEEECCCCCCCCCCCCCCCCCH
YDLFDLGEFDQKGSVRTKYGTRQQYLDAVKSLQTHGLQVYADAVLNHKMGGDAVETPKAT
HHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCCCCCCCC
PFPQDDRLNPKGGLQDIKTYTHYNFPGRQGKYSNFEWHWWHFDAVDYNEYNSGDRSTVYL
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEE
LEGKNFDDYVALEKGNFAYLMGCDLDFQNEWVRGEVTYWGKWCLDTTKVDGFRIDAIKHI
EECCCCCCEEEEECCCEEEEEECCCCCCCCEEEEEEEEEEEEEEECCCCCCEEEHHHHHH
STWFFPEWIDALERHAGKDLFMVGEYWYNDINTLLWYVDAVRGKMSVFDVPLHYNFHQAS
HHHCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCHHHHC
KSGGNYDMRRILDGTMMQQRPTHAVTFVENHDSQPLQALESVVEPWFKPLAYAIILLRQE
CCCCCHHHHHHHCCHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEECC
GYPCVFHADYYGAEYEDWGKDGNRYNIFMPSHRWIIDKLLYARKHYAYGPQYNYLDHWNT
CCCEEEECCCCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
IGWTRLGDADHPQGMAVIMSDGSEGIKWMEVGKPNTKFIDLTEHIKEAVYTNEWGWGEFR
CCEEECCCCCCCCCEEEEEECCCCCCEEEECCCCCCEEEEHHHHHHHHHHCCCCCCCEEE
CLGGSVSVWVQA
EECCCEEEEEEC
>Mature Secondary Structure 
AQMNGTMMQYFHWYIPNDGNLWSKVEASAPELADAGFTAMWLPPAYKGFAGSFDVGYGV
CCCCCCEEEEEEEEECCCCCEEEECCCCCCCHHCCCCEEEECCCCCCCCCCCCCCCCCH
YDLFDLGEFDQKGSVRTKYGTRQQYLDAVKSLQTHGLQVYADAVLNHKMGGDAVETPKAT
HHHHCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCCCCCCCC
PFPQDDRLNPKGGLQDIKTYTHYNFPGRQGKYSNFEWHWWHFDAVDYNEYNSGDRSTVYL
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEE
LEGKNFDDYVALEKGNFAYLMGCDLDFQNEWVRGEVTYWGKWCLDTTKVDGFRIDAIKHI
EECCCCCCEEEEECCCEEEEEECCCCCCCCEEEEEEEEEEEEEEECCCCCCEEEHHHHHH
STWFFPEWIDALERHAGKDLFMVGEYWYNDINTLLWYVDAVRGKMSVFDVPLHYNFHQAS
HHHCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCHHHHC
KSGGNYDMRRILDGTMMQQRPTHAVTFVENHDSQPLQALESVVEPWFKPLAYAIILLRQE
CCCCCHHHHHHHCCHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEECC
GYPCVFHADYYGAEYEDWGKDGNRYNIFMPSHRWIIDKLLYARKHYAYGPQYNYLDHWNT
CCCEEEECCCCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
IGWTRLGDADHPQGMAVIMSDGSEGIKWMEVGKPNTKFIDLTEHIKEAVYTNEWGWGEFR
CCEEECCCCCCCCCEEEEEECCCCCCEEEECCCCCCEEEEHHHHHHHHHHCCCCCCCEEE
CLGGSVSVWVQA
EECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3924897; 3876333; 3009417; 2999073; 11226887 [H]