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The map label for this gene is kinB [H]

Identifier: 17229586

GI number: 17229586

Start: 2508306

End: 2510021

Strand: Reverse

Name: kinB [H]

Synonym: all2094

Alternate gene names: 17229586

Gene position: 2510021-2508306 (Counterclockwise)

Preceding gene: 17229587

Following gene: 17229583

Centisome position: 39.13

GC content: 38.64

Gene sequence:

>1716_bases
ATGATTAGCATCTGTTCACAGACCAATAAAATCCAAGCGGTCAGAGTGCTGATTGTGGAAGATGAATATATCTTGGCGAT
TAACTTACAAGAAAGTTTAGAGTCTTTAGGATATACAGTTGTAGATATTGCAGATTCCGCAGAGTTAGCCATAGATAAAG
CGACAGAACTACAGCCAAACTTAATTTTGATGGATATCAGGCTACGAGGTGAAGAAGATGGTATTCATGCAGCCGAAAAA
ATCTGGCAGAATCTACAAATTCCCATCATATATGTGACAGGACATTCTGATAAAAGCACTGTGGAACGAGCAACGCTCAC
GTCCCCTTTTGGCTACATCCTCAAACCCGTCAGAGAAAAAGAACTCTACGTCGCTATTCAAACAGCTCTCAATCGCTATG
AACGTGAACAATTTCTGAGTTCCGTGCTGCGAGTTATGGGAGATGGTGTACTAGTGGTTGATTCTCAGTTGCACATCAAG
TATATGAATCAAGTCGCAGAAGCACTTACCGGTTGGCAATTTGATGATGCACAAGATAGAAATTTAACTGAAGTTGTCCA
ATTAATTGATGAACAAACTCTCAATTCCATTCAAAATCCTATCATCTCAGCTATTCAAAAAGAAACAACGGTTTATTTAG
GTGATGGCCTTTCGCCGACAGTCGGTCATCGCATCATGTTAATTACCAAAGATGGCACAATGATCCCAGTTGCCGATAGT
GCTACCCCCCTACGCAACAACAACGGTGATATTACTGGCGCAGTCATGGTTTTTCGGGACGACACTCAACGCCGACTCAC
AGAAGAACGCAATCTCTCTGCTGAACGCAATCGACAACTAGAAATTCAAATGGCAGAAATGCAGCGATTCAACCAGTTGA
AAGAAGACTTTTTAGCATCTACCTCCCATGAAATGAGAACGCCATTATCAAATATCAAAATGGCTATTTCCATGCTGGAA
AATGTCATGAATCAGCAGGGTCTTTTGAAATCAGCCACCGCTTCTACTTCCGCATCCGTGTCTCGTTACCTGGGTATTTT
ACGTTATGAGTGCGAACGGGAATTAACTTTAGTAGATGATCTAATTAATATGCGGATAATTGATACAGAAGGCTATCCTT
TAGATATCACTTATATTCATCTACAATCTTTGCTATCCGAAATTATTAACAGTTTTCAGGAAATTACCAAAGCGCAACAA
CAGATTTTAGAAGGTAATATTTCTGATGATTTACCAGCTTTAGTTTCTGACAAAAGTATCCTCAATCGAATCATCTCAGA
ACTCCTCATGAATGCTTGTAAATATACTCCTCCTGGTGAGCGCATTACCATAACAGCACATTTTGAGCAAAACCAAAAAA
GCTTAACACCACAAGATGCTCAATCTGGTTTATACACAGTTTCTTCATCTCCTTATTTTCAAATTAAAATTAACAACTCT
GGAGTAGAAATTCCTTCAACAGAACAATCTCGGATTTTTGAGCCGTTCTATCGATTTACCTACAATACAACAATAGAAAA
ATTATCTGTGTTTGACCAATCTTACCAAATTCCCCAAAGTGATTATCAACGAACTAGTGGTACAGGTTTGGGATTATCAC
TAGTGAAAAAACTAGTACAGTATATTCAAGGCACAGTTGAAGTTAGAAGTGGTGATGGCTTAACTACTTTTATAGTTCAA
TTGCCATTGACTTTATCAGTGAATTATTCTAGATAA

Upstream 100 bases:

>100_bases
TTGTTCAGGGTTTAGTCCAGCAGTTAAGAGGCAAATTAGAAATTCAGAGTCAGTCAGGAACTGAATTTAAAATTTCTTTT
ACGACAGGTAGGGTATAAGC

Downstream 100 bases:

>100_bases
GTAAACTATTTCTTACTATGAGTTGCTCATCATCATGTTTTTTGAAAAACTTCTTAATATCTATAATTGATAAACATCCT
ATTCCCTTGCTTGTATTGAG

Product: two-component hybrid sensor and regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MISICSQTNKIQAVRVLIVEDEYILAINLQESLESLGYTVVDIADSAELAIDKATELQPNLILMDIRLRGEEDGIHAAEK
IWQNLQIPIIYVTGHSDKSTVERATLTSPFGYILKPVREKELYVAIQTALNRYEREQFLSSVLRVMGDGVLVVDSQLHIK
YMNQVAEALTGWQFDDAQDRNLTEVVQLIDEQTLNSIQNPIISAIQKETTVYLGDGLSPTVGHRIMLITKDGTMIPVADS
ATPLRNNNGDITGAVMVFRDDTQRRLTEERNLSAERNRQLEIQMAEMQRFNQLKEDFLASTSHEMRTPLSNIKMAISMLE
NVMNQQGLLKSATASTSASVSRYLGILRYECERELTLVDDLINMRIIDTEGYPLDITYIHLQSLLSEIINSFQEITKAQQ
QILEGNISDDLPALVSDKSILNRIISELLMNACKYTPPGERITITAHFEQNQKSLTPQDAQSGLYTVSSSPYFQIKINNS
GVEIPSTEQSRIFEPFYRFTYNTTIEKLSVFDQSYQIPQSDYQRTSGTGLGLSLVKKLVQYIQGTVEVRSGDGLTTFIVQ
LPLTLSVNYSR

Sequences:

>Translated_571_residues
MISICSQTNKIQAVRVLIVEDEYILAINLQESLESLGYTVVDIADSAELAIDKATELQPNLILMDIRLRGEEDGIHAAEK
IWQNLQIPIIYVTGHSDKSTVERATLTSPFGYILKPVREKELYVAIQTALNRYEREQFLSSVLRVMGDGVLVVDSQLHIK
YMNQVAEALTGWQFDDAQDRNLTEVVQLIDEQTLNSIQNPIISAIQKETTVYLGDGLSPTVGHRIMLITKDGTMIPVADS
ATPLRNNNGDITGAVMVFRDDTQRRLTEERNLSAERNRQLEIQMAEMQRFNQLKEDFLASTSHEMRTPLSNIKMAISMLE
NVMNQQGLLKSATASTSASVSRYLGILRYECERELTLVDDLINMRIIDTEGYPLDITYIHLQSLLSEIINSFQEITKAQQ
QILEGNISDDLPALVSDKSILNRIISELLMNACKYTPPGERITITAHFEQNQKSLTPQDAQSGLYTVSSSPYFQIKINNS
GVEIPSTEQSRIFEPFYRFTYNTTIEKLSVFDQSYQIPQSDYQRTSGTGLGLSLVKKLVQYIQGTVEVRSGDGLTTFIVQ
LPLTLSVNYSR
>Mature_571_residues
MISICSQTNKIQAVRVLIVEDEYILAINLQESLESLGYTVVDIADSAELAIDKATELQPNLILMDIRLRGEEDGIHAAEK
IWQNLQIPIIYVTGHSDKSTVERATLTSPFGYILKPVREKELYVAIQTALNRYEREQFLSSVLRVMGDGVLVVDSQLHIK
YMNQVAEALTGWQFDDAQDRNLTEVVQLIDEQTLNSIQNPIISAIQKETTVYLGDGLSPTVGHRIMLITKDGTMIPVADS
ATPLRNNNGDITGAVMVFRDDTQRRLTEERNLSAERNRQLEIQMAEMQRFNQLKEDFLASTSHEMRTPLSNIKMAISMLE
NVMNQQGLLKSATASTSASVSRYLGILRYECERELTLVDDLINMRIIDTEGYPLDITYIHLQSLLSEIINSFQEITKAQQ
QILEGNISDDLPALVSDKSILNRIISELLMNACKYTPPGERITITAHFEQNQKSLTPQDAQSGLYTVSSSPYFQIKINNS
GVEIPSTEQSRIFEPFYRFTYNTTIEKLSVFDQSYQIPQSDYQRTSGTGLGLSLVKKLVQYIQGTVEVRSGDGLTTFIVQ
LPLTLSVNYSR

Specific function: Member of the two-component regulatory system AlgB/KinB involved in regulation of alginate biosynthesis genes. KinB functions as a membrane-associated protein kinase that phosphorylates AlgB, probably in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=291, Percent_Identity=26.4604810996564, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1789149, Length=334, Percent_Identity=23.9520958083832, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788713, Length=311, Percent_Identity=24.4372990353698, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1786783, Length=269, Percent_Identity=24.5353159851301, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87081816, Length=289, Percent_Identity=23.5294117647059, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI48994928, Length=282, Percent_Identity=23.758865248227, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF08448 PAS_4 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 64316; Mature: 64316

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISICSQTNKIQAVRVLIVEDEYILAINLQESLESLGYTVVDIADSAELAIDKATELQPN
CCCCCCCCCCEEEEEEEEEECCEEEEEEHHHHHHHCCCEEEEECCCCHHEECCCCCCCCC
LILMDIRLRGEEDGIHAAEKIWQNLQIPIIYVTGHSDKSTVERATLTSPFGYILKPVREK
EEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHCCCCHHHHHHHCCHH
ELYVAIQTALNRYEREQFLSSVLRVMGDGVLVVDSQLHIKYMNQVAEALTGWQFDDAQDR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCC
NLTEVVQLIDEQTLNSIQNPIISAIQKETTVYLGDGLSPTVGHRIMLITKDGTMIPVADS
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCCCEEEECCC
ATPLRNNNGDITGAVMVFRDDTQRRLTEERNLSAERNRQLEIQMAEMQRFNQLKEDFLAS
CCCCCCCCCCEEEEEEEEECCHHHHHHHHHCCCHHCCCEEEEEHHHHHHHHHHHHHHHHC
TSHEMRTPLSNIKMAISMLENVMNQQGLLKSATASTSASVSRYLGILRYECERELTLVDD
CCHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHH
LINMRIIDTEGYPLDITYIHLQSLLSEIINSFQEITKAQQQILEGNISDDLPALVSDKSI
HHCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHH
LNRIISELLMNACKYTPPGERITITAHFEQNQKSLTPQDAQSGLYTVSSSPYFQIKINNS
HHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCHHCCCEEEECCCCEEEEEECCC
GVEIPSTEQSRIFEPFYRFTYNTTIEKLSVFDQSYQIPQSDYQRTSGTGLGLSLVKKLVQ
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHH
YIQGTVEVRSGDGLTTFIVQLPLTLSVNYSR
HHCCEEEEECCCCCEEEEEEECEEEEEECCC
>Mature Secondary Structure
MISICSQTNKIQAVRVLIVEDEYILAINLQESLESLGYTVVDIADSAELAIDKATELQPN
CCCCCCCCCCEEEEEEEEEECCEEEEEEHHHHHHHCCCEEEEECCCCHHEECCCCCCCCC
LILMDIRLRGEEDGIHAAEKIWQNLQIPIIYVTGHSDKSTVERATLTSPFGYILKPVREK
EEEEEEEEECCCCCHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHCCCCHHHHHHHCCHH
ELYVAIQTALNRYEREQFLSSVLRVMGDGVLVVDSQLHIKYMNQVAEALTGWQFDDAQDR
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCC
NLTEVVQLIDEQTLNSIQNPIISAIQKETTVYLGDGLSPTVGHRIMLITKDGTMIPVADS
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEEECCCCEEEECCC
ATPLRNNNGDITGAVMVFRDDTQRRLTEERNLSAERNRQLEIQMAEMQRFNQLKEDFLAS
CCCCCCCCCCEEEEEEEEECCHHHHHHHHHCCCHHCCCEEEEEHHHHHHHHHHHHHHHHC
TSHEMRTPLSNIKMAISMLENVMNQQGLLKSATASTSASVSRYLGILRYECERELTLVDD
CCHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHH
LINMRIIDTEGYPLDITYIHLQSLLSEIINSFQEITKAQQQILEGNISDDLPALVSDKSI
HHCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHH
LNRIISELLMNACKYTPPGERITITAHFEQNQKSLTPQDAQSGLYTVSSSPYFQIKINNS
HHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCHHCCCEEEECCCCEEEEEECCC
GVEIPSTEQSRIFEPFYRFTYNTTIEKLSVFDQSYQIPQSDYQRTSGTGLGLSLVKKLVQ
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHH
YIQGTVEVRSGDGLTTFIVQLPLTLSVNYSR
HHCCEEEEECCCCCEEEEEEECEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9218420 [H]