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The map label for this gene is cobD [H]

Identifier: 17229574

GI number: 17229574

Start: 2489250

End: 2490212

Strand: Direct

Name: cobD [H]

Synonym: alr2082

Alternate gene names: 17229574

Gene position: 2489250-2490212 (Clockwise)

Preceding gene: 17229573

Following gene: 17229575

Centisome position: 38.81

GC content: 43.3

Gene sequence:

>963_bases
ATGACTAATAACATCTATGTCTTAATCATTGCTGTAATTTTAGATTATTTAATAGGTGATCCTTGGGGTTGGCCTCATCC
GGTGCAAGTTATGGGATGGGGAATTTCATGGTTGAGCAAAATTTTTCTCCAACTGTGTCAAAAATCTCTCACACAACGCA
TCGCTGGGATTATTCTTGCAATAATTTTGATTGTTGGTAGCGGTGGGGTTGGTTGGTTAATCATTCAAATGACGAGATGG
CTAAATCCTGTTTTAGCGATCGCACTAGAAAGTATCATGTTAGCTAGTTGTTTTGCTTTGAAAAGTCTAGGCAAAGCAGC
TATTGATGTTTTACAACCTTTAAAAGCAGGCGATTTAACAGTAGCTCGGCAGACTTTGAGTAATTATGTTGGTCGAGATA
CAGATAACTTACCAGAATCGGAAATCCTCCGAGCAGTTTTAGAAACAGTGACAGAGAATGCGACAGATGGGGTAATGGCT
CCACTGTTTTATGCTATTGTCGGTATCTGCATTCCCCATGTAGGGCCAGTTCCTCTGGCTTTGGCATATAAAGCTAGCAG
TACCCTCGATTCGATGGTGGGTTACAAAGAAGCACCATATACATATTTAGGATGGTTCAGTGCGCGGCTAGAAGATTACT
TAACTTGGTTACCTTGCCGCTTGACAGTGATAACTTTAGGATTATTATCAGGGAAGCCTGGGGATGTGTGGCGAATTTGT
CGCCGTGATGCAATTAATGATCCTAGTCCCAACTCAGGTTGGAGCGAGTGCGCCTATGCAGCCATTTTGGGTGTGCAGAT
GGGGGGGACAAATTGGTATGGTGGGATAGCCAAGCAAAAACCGCTCTTAGGAGATCCCACTTCTCCCATCAACCCACAAC
ACATTCAAATAGCTTTGCAACTGACTAGATATAGCTTTTTGCTCTGGCTAGGTGTGGCGGTCGCTATACTCCTGATACGC
TAA

Upstream 100 bases:

>100_bases
TTTGTATTACGATGCCCGTAGCTGGCAAGCTTCTCTCGGAGAAGAAGCAAATTTTGTGGTTTAGCAATCGTCAGTTGTCG
GTTGACATTTGTTTTCCTTC

Downstream 100 bases:

>100_bases
AGGTATGAGGGAGAAGCAGTAGGGGAGCAGAAGGGAAAACTTGCTCAAACATTTCCCCCCTTTCCCCCTGCCTCAATTCC
TAACTTCGCCCACTCCCTAT

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTNNIYVLIIAVILDYLIGDPWGWPHPVQVMGWGISWLSKIFLQLCQKSLTQRIAGIILAIILIVGSGGVGWLIIQMTRW
LNPVLAIALESIMLASCFALKSLGKAAIDVLQPLKAGDLTVARQTLSNYVGRDTDNLPESEILRAVLETVTENATDGVMA
PLFYAIVGICIPHVGPVPLALAYKASSTLDSMVGYKEAPYTYLGWFSARLEDYLTWLPCRLTVITLGLLSGKPGDVWRIC
RRDAINDPSPNSGWSECAYAAILGVQMGGTNWYGGIAKQKPLLGDPTSPINPQHIQIALQLTRYSFLLWLGVAVAILLIR

Sequences:

>Translated_320_residues
MTNNIYVLIIAVILDYLIGDPWGWPHPVQVMGWGISWLSKIFLQLCQKSLTQRIAGIILAIILIVGSGGVGWLIIQMTRW
LNPVLAIALESIMLASCFALKSLGKAAIDVLQPLKAGDLTVARQTLSNYVGRDTDNLPESEILRAVLETVTENATDGVMA
PLFYAIVGICIPHVGPVPLALAYKASSTLDSMVGYKEAPYTYLGWFSARLEDYLTWLPCRLTVITLGLLSGKPGDVWRIC
RRDAINDPSPNSGWSECAYAAILGVQMGGTNWYGGIAKQKPLLGDPTSPINPQHIQIALQLTRYSFLLWLGVAVAILLIR
>Mature_319_residues
TNNIYVLIIAVILDYLIGDPWGWPHPVQVMGWGISWLSKIFLQLCQKSLTQRIAGIILAIILIVGSGGVGWLIIQMTRWL
NPVLAIALESIMLASCFALKSLGKAAIDVLQPLKAGDLTVARQTLSNYVGRDTDNLPESEILRAVLETVTENATDGVMAP
LFYAIVGICIPHVGPVPLALAYKASSTLDSMVGYKEAPYTYLGWFSARLEDYLTWLPCRLTVITLGLLSGKPGDVWRICR
RDAINDPSPNSGWSECAYAAILGVQMGGTNWYGGIAKQKPLLGDPTSPINPQHIQIALQLTRYSFLLWLGVAVAILLIR

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 34960; Mature: 34828

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNIYVLIIAVILDYLIGDPWGWPHPVQVMGWGISWLSKIFLQLCQKSLTQRIAGIILA
CCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IILIVGSGGVGWLIIQMTRWLNPVLAIALESIMLASCFALKSLGKAAIDVLQPLKAGDLT
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VARQTLSNYVGRDTDNLPESEILRAVLETVTENATDGVMAPLFYAIVGICIPHVGPVPLA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
LAYKASSTLDSMVGYKEAPYTYLGWFSARLEDYLTWLPCRLTVITLGLLSGKPGDVWRIC
EHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
RRDAINDPSPNSGWSECAYAAILGVQMGGTNWYGGIAKQKPLLGDPTSPINPQHIQIALQ
HHHCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHH
LTRYSFLLWLGVAVAILLIR
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNNIYVLIIAVILDYLIGDPWGWPHPVQVMGWGISWLSKIFLQLCQKSLTQRIAGIILA
CCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IILIVGSGGVGWLIIQMTRWLNPVLAIALESIMLASCFALKSLGKAAIDVLQPLKAGDLT
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
VARQTLSNYVGRDTDNLPESEILRAVLETVTENATDGVMAPLFYAIVGICIPHVGPVPLA
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCE
LAYKASSTLDSMVGYKEAPYTYLGWFSARLEDYLTWLPCRLTVITLGLLSGKPGDVWRIC
EHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
RRDAINDPSPNSGWSECAYAAILGVQMGGTNWYGGIAKQKPLLGDPTSPINPQHIQIALQ
HHHCCCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHH
LTRYSFLLWLGVAVAILLIR
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]