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The map label for this gene is nemA [H]

Identifier: 17229357

GI number: 17229357

Start: 2227714

End: 2228922

Strand: Reverse

Name: nemA [H]

Synonym: all1865

Alternate gene names: 17229357

Gene position: 2228922-2227714 (Counterclockwise)

Preceding gene: 17229358

Following gene: 17229356

Centisome position: 34.75

GC content: 45.41

Gene sequence:

>1209_bases
GTGTCTGATGAAGCAGAGAGGCAAAGGGGAAATAACCTGTATAAGAACTCTCCTCTGCTCCCTGTTTCTATCTCTCAAGT
ATCCACATCCCAATTACGAGAAACTGAGATTATGTCTACCAACATCAACCTATTCTCTTCCTACCAATTGGGAGAACTAG
AATTACCCAACCGGATAGTGATGGCTCCTTTAACCAGACAAAGAGCTGGTGAGGGAAATGTACCGCATCAACTTAATGCT
ATCTACTATGGTCAACGTGCTTCAGCAGGATTAATTATTGCCGAAGCTACCCAGGTGACACCCCAAGGACAAGGGTATCC
CCATACACCAGGCATCCATTCCCCAGAACAAGTAGCAGGGTGGAAACTAGTAACTGATACTGTACATCAACAAGGGGGCA
GAATTTTTCTGCAACTGTGGCACGTCGGCAGAATATCTCACCCTGACTTGCAGCCAGATGGGGGATTACCTGTAGCACCT
TCAGCCATTGCGCCTAAAGGTGAGGTATTAACTTATGAAGGCAAGAAACCTTATGTTACGCCTCGTGCTTTGGACACATC
AGAAATTCCCGCTATTGTCGAACAATATCGCCAAGGGGCTGCAAACGCCCTAGCGGCTGGGTTTGATGGTGTAGAAATTC
ATGCTGCCAATGGTTATTTAATCGACCAGTTTCTTAGAGATGGGACGAATCAACGTACAGACGAGTATGGGGGTGCGATT
GAGAATCGCGCCCGACTTTTATTAGAAGTAACCGAGGCGATAACAAGTGTGTGGGATTCCCAAAGGGTAGGAGTCAGACT
ATCGCCTAGTGGGACATTTAACGATATCCGTGACTCTCATCCTTTAGAAACATTTGGTTACGTAGCTCAAGCACTGAACC
GCTTCAACTTATCCTATTTACATATCTTTGAAGCTATAGACGCAGACATTAGACATGGTGGCACAGTTGTACCTACTAGC
CATCTACGCGATCGCTTTACAGGTACACTCATTGTTAATGGTGGTTATACCCGTGAAAAAGGCGATACTGTCATAGCCAA
CAAAGCAGCAGATTTAGTTGCCTTTGGGACGCTGTTTATCTCCAATCCTGATTTACCTGAACGCCTAGAGGTGAACGCAC
CACTCAATCAGGCAGACCCCACAACCTTTTATGGTGGTGGTGAAAAAGGCTATACAGATTATCCATTTTTAGCAGTTGCT
AATAAGTAA

Upstream 100 bases:

>100_bases
AAGTTAATACAGTTGTAGGGGCTGTTTCCAAGACCACATTGACTAAGACTTTAACACAGCATATTCAAGAAGCGTAACCC
GCATTTCTCACAAGCTTTAG

Downstream 100 bases:

>100_bases
GAGGTTGTAACCATAGGCTAGGCAGTCTCAATGAGACTTGTTTCATGGACACAGAGGAAAGACATCTTTTCTCTACACCC
CTACACCCTGTTTCCAACAT

Product: hypothetical protein

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSDEAERQRGNNLYKNSPLLPVSISQVSTSQLRETEIMSTNINLFSSYQLGELELPNRIVMAPLTRQRAGEGNVPHQLNA
IYYGQRASAGLIIAEATQVTPQGQGYPHTPGIHSPEQVAGWKLVTDTVHQQGGRIFLQLWHVGRISHPDLQPDGGLPVAP
SAIAPKGEVLTYEGKKPYVTPRALDTSEIPAIVEQYRQGAANALAAGFDGVEIHAANGYLIDQFLRDGTNQRTDEYGGAI
ENRARLLLEVTEAITSVWDSQRVGVRLSPSGTFNDIRDSHPLETFGYVAQALNRFNLSYLHIFEAIDADIRHGGTVVPTS
HLRDRFTGTLIVNGGYTREKGDTVIANKAADLVAFGTLFISNPDLPERLEVNAPLNQADPTTFYGGGEKGYTDYPFLAVA
NK

Sequences:

>Translated_402_residues
MSDEAERQRGNNLYKNSPLLPVSISQVSTSQLRETEIMSTNINLFSSYQLGELELPNRIVMAPLTRQRAGEGNVPHQLNA
IYYGQRASAGLIIAEATQVTPQGQGYPHTPGIHSPEQVAGWKLVTDTVHQQGGRIFLQLWHVGRISHPDLQPDGGLPVAP
SAIAPKGEVLTYEGKKPYVTPRALDTSEIPAIVEQYRQGAANALAAGFDGVEIHAANGYLIDQFLRDGTNQRTDEYGGAI
ENRARLLLEVTEAITSVWDSQRVGVRLSPSGTFNDIRDSHPLETFGYVAQALNRFNLSYLHIFEAIDADIRHGGTVVPTS
HLRDRFTGTLIVNGGYTREKGDTVIANKAADLVAFGTLFISNPDLPERLEVNAPLNQADPTTFYGGGEKGYTDYPFLAVA
NK
>Mature_401_residues
SDEAERQRGNNLYKNSPLLPVSISQVSTSQLRETEIMSTNINLFSSYQLGELELPNRIVMAPLTRQRAGEGNVPHQLNAI
YYGQRASAGLIIAEATQVTPQGQGYPHTPGIHSPEQVAGWKLVTDTVHQQGGRIFLQLWHVGRISHPDLQPDGGLPVAPS
AIAPKGEVLTYEGKKPYVTPRALDTSEIPAIVEQYRQGAANALAAGFDGVEIHAANGYLIDQFLRDGTNQRTDEYGGAIE
NRARLLLEVTEAITSVWDSQRVGVRLSPSGTFNDIRDSHPLETFGYVAQALNRFNLSYLHIFEAIDADIRHGGTVVPTSH
LRDRFTGTLIVNGGYTREKGDTVIANKAADLVAFGTLFISNPDLPERLEVNAPLNQADPTTFYGGGEKGYTDYPFLAVAN
K

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=370, Percent_Identity=48.1081081081081, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1789463, Length=225, Percent_Identity=32.8888888888889, Blast_Score=101, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17559804, Length=260, Percent_Identity=25.3846153846154, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI72001454, Length=275, Percent_Identity=26.5454545454545, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17559802, Length=129, Percent_Identity=39.5348837209302, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17565138, Length=342, Percent_Identity=26.3157894736842, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17564188, Length=260, Percent_Identity=24.6153846153846, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17540738, Length=258, Percent_Identity=27.906976744186, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17566914, Length=97, Percent_Identity=39.1752577319588, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6325086, Length=379, Percent_Identity=39.0501319261214, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6321973, Length=385, Percent_Identity=37.6623376623377, Blast_Score=214, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 43903; Mature: 43771

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDEAERQRGNNLYKNSPLLPVSISQVSTSQLRETEIMSTNINLFSSYQLGELELPNRIV
CCCHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHEECCCCEEECCCCCCCCCCCEEE
MAPLTRQRAGEGNVPHQLNAIYYGQRASAGLIIAEATQVTPQGQGYPHTPGIHSPEQVAG
ECCCHHHCCCCCCCCCEEEEEEECCCCCCCEEEEECEECCCCCCCCCCCCCCCCHHHHCC
WKLVTDTVHQQGGRIFLQLWHVGRISHPDLQPDGGLPVAPSAIAPKGEVLTYEGKKPYVT
EEEEHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
PRALDTSEIPAIVEQYRQGAANALAAGFDGVEIHAANGYLIDQFLRDGTNQRTDEYGGAI
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCHHHCCHH
ENRARLLLEVTEAITSVWDSQRVGVRLSPSGTFNDIRDSHPLETFGYVAQALNRFNLSYL
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHCCCCCHHHHHHHHHHHHHCCCHHH
HIFEAIDADIRHGGTVVPTSHLRDRFTGTLIVNGGYTREKGDTVIANKAADLVAFGTLFI
HHHHHHHHHHHCCCEECCHHHHHHCCCEEEEEECCCCCCCCCEEEECCHHHHEEEEEEEE
SNPDLPERLEVNAPLNQADPTTFYGGGEKGYTDYPFLAVANK
CCCCCCCEEEECCCCCCCCCCEEECCCCCCCCCCCEEEEECC
>Mature Secondary Structure 
SDEAERQRGNNLYKNSPLLPVSISQVSTSQLRETEIMSTNINLFSSYQLGELELPNRIV
CCHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHEECCCCEEECCCCCCCCCCCEEE
MAPLTRQRAGEGNVPHQLNAIYYGQRASAGLIIAEATQVTPQGQGYPHTPGIHSPEQVAG
ECCCHHHCCCCCCCCCEEEEEEECCCCCCCEEEEECEECCCCCCCCCCCCCCCCHHHHCC
WKLVTDTVHQQGGRIFLQLWHVGRISHPDLQPDGGLPVAPSAIAPKGEVLTYEGKKPYVT
EEEEHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
PRALDTSEIPAIVEQYRQGAANALAAGFDGVEIHAANGYLIDQFLRDGTNQRTDEYGGAI
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCCCHHHCCHH
ENRARLLLEVTEAITSVWDSQRVGVRLSPSGTFNDIRDSHPLETFGYVAQALNRFNLSYL
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHCCCCCHHHHHHHHHHHHHCCCHHH
HIFEAIDADIRHGGTVVPTSHLRDRFTGTLIVNGGYTREKGDTVIANKAADLVAFGTLFI
HHHHHHHHHHHCCCEECCHHHHHHCCCEEEEEECCCCCCCCCEEEECCHHHHEEEEEEEE
SNPDLPERLEVNAPLNQADPTTFYGGGEKGYTDYPFLAVANK
CCCCCCCEEEECCCCCCCCCCEEECCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]