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The map label for this gene is galE [C]

Identifier: 17229236

GI number: 17229236

Start: 2098495

End: 2099649

Strand: Direct

Name: galE [C]

Synonym: alr1744

Alternate gene names: 17229236

Gene position: 2098495-2099649 (Clockwise)

Preceding gene: 17229234

Following gene: 17229238

Centisome position: 32.72

GC content: 42.6

Gene sequence:

>1155_bases
ATGAAAGTCCTAGTTATTGGTGGCGATGGATATTGCGGTTGGGCAACCGCACTTTATCTTTCCAATCGCGGTTATGAAGT
TGGAATATTAGATAGTTTGGTGCGTCGGCACTGGGATAATGAACTGGGTATCGAAACTCTGACTCCGATCGCACCAATTC
AGCAACGTCTCCAGCGCTGGCAAGATTTAACAGGCAAATCTATTGACCTGTTTGTTGGCGATATTACCAACTATGAATTT
CTCCAAAAAGCGTTGCATAAATTTGAGCCAAATGCCATAGTCCATTTTGGCGAACAGCGTTCAGCACCATTTTCGATGAT
TGACCGCGAACACGCAGTTGTTACCCAGGTCAATAACGTTGTGGGTACGTTGAATTTGCTATATGCAATGAAGGAAGATT
TTCCTGACTGTCACCTAGTGAAGTTAGGAACAATGGGGGAATACGGCACACCTAATATTGATATCGAAGAAGGCTACATC
ACCATTGAACACAATGGGCGCAAGGATACCCTACCTTATCCCAAGCAACCAGGTTCAATGTACCACTTGAGTAAAGTTCA
TGATAGCCACAATATCCATTTTGCTTGTCGGATTTGGGGATTGCGGGCAACAGACTTAAATCAAGGTGTGGTTTACGGCG
TTCTCACCGAAGAAACGGGGATGGACGAACTCTTAATTAACCGACTAGATTACGATGGTGTGTTTGGTACAGCGTTAAAC
CGTTTCTGTATTCAAGCTGCAACAGGTCATCCCCTAACTGTTTATGGTACAGGTGGACAAACGCGCGGATTCTTGGATAT
TCGGGATACGGTGCGATGTGTGGAACTAGCGATCGCTAATCCGGCTCCATCGGGTGAGTTTCGTGTTTTTAACCAATTTA
CTGAACAATTCAGTGTTGGTGACTTGGCATTAATGGTGAAAAAAGCTGGCAATGCCTTGGGATTAAATGTAGAGATCAAT
AATCTCGATAATCCCAGAATCGAGAAAGAAGAACATTACTTCAATGCCAAAAACACTAAACTACTCGATTTAGGTTTGCA
ACCTCATTATCTCTCTGATTCTCTGCTAGATTCTCTCTTAAACTTTGCTGTCAAGTATCAAGGACGAGTTGATCAAAAGC
AAATTTTACCTAAAGTTTCTTGGCATAGAAAATAA

Upstream 100 bases:

>100_bases
AAATGAGGAGTCCTTTCTGATTCCACATCTTCCAGAATGATGAGTGAGGGGGTACCCAAACCTGATAGATTAAGCTATCA
GCGAGTAAAAACTGGTGAAA

Downstream 100 bases:

>100_bases
AAATAACACTGAAGCAAAAAGCTTTTTTGTTCTTCCAGGGTGTAAAAGTATGAGATTTTTCAACATCTAAACTCTCATAC
CTTTACACCCTATTTTTAGA

Product: sulfolipid biosynthesis protein

Products: UDPglucoseal [C]

Alternate protein names: NAD-Dependent Epimerase/Dehydratase; Sulfolipid Biosynthesis Protein; NAD Dependent Epimerase/Dehydratase Family; Sulfolipid Biosynthesis Protein SqdB; Nucleoside-Diphosphate-Sugar Epimerase; Epimerase; UDP Sulfoquinovose Synthase; NAD Dependent Epimerase/Dehydratase Family Protein

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MKVLVIGGDGYCGWATALYLSNRGYEVGILDSLVRRHWDNELGIETLTPIAPIQQRLQRWQDLTGKSIDLFVGDITNYEF
LQKALHKFEPNAIVHFGEQRSAPFSMIDREHAVVTQVNNVVGTLNLLYAMKEDFPDCHLVKLGTMGEYGTPNIDIEEGYI
TIEHNGRKDTLPYPKQPGSMYHLSKVHDSHNIHFACRIWGLRATDLNQGVVYGVLTEETGMDELLINRLDYDGVFGTALN
RFCIQAATGHPLTVYGTGGQTRGFLDIRDTVRCVELAIANPAPSGEFRVFNQFTEQFSVGDLALMVKKAGNALGLNVEIN
NLDNPRIEKEEHYFNAKNTKLLDLGLQPHYLSDSLLDSLLNFAVKYQGRVDQKQILPKVSWHRK

Sequences:

>Translated_384_residues
MKVLVIGGDGYCGWATALYLSNRGYEVGILDSLVRRHWDNELGIETLTPIAPIQQRLQRWQDLTGKSIDLFVGDITNYEF
LQKALHKFEPNAIVHFGEQRSAPFSMIDREHAVVTQVNNVVGTLNLLYAMKEDFPDCHLVKLGTMGEYGTPNIDIEEGYI
TIEHNGRKDTLPYPKQPGSMYHLSKVHDSHNIHFACRIWGLRATDLNQGVVYGVLTEETGMDELLINRLDYDGVFGTALN
RFCIQAATGHPLTVYGTGGQTRGFLDIRDTVRCVELAIANPAPSGEFRVFNQFTEQFSVGDLALMVKKAGNALGLNVEIN
NLDNPRIEKEEHYFNAKNTKLLDLGLQPHYLSDSLLDSLLNFAVKYQGRVDQKQILPKVSWHRK
>Mature_384_residues
MKVLVIGGDGYCGWATALYLSNRGYEVGILDSLVRRHWDNELGIETLTPIAPIQQRLQRWQDLTGKSIDLFVGDITNYEF
LQKALHKFEPNAIVHFGEQRSAPFSMIDREHAVVTQVNNVVGTLNLLYAMKEDFPDCHLVKLGTMGEYGTPNIDIEEGYI
TIEHNGRKDTLPYPKQPGSMYHLSKVHDSHNIHFACRIWGLRATDLNQGVVYGVLTEETGMDELLINRLDYDGVFGTALN
RFCIQAATGHPLTVYGTGGQTRGFLDIRDTVRCVELAIANPAPSGEFRVFNQFTEQFSVGDLALMVKKAGNALGLNVEIN
NLDNPRIEKEEHYFNAKNTKLLDLGLQPHYLSDSLLDSLLNFAVKYQGRVDQKQILPKVSWHRK

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 43264; Mature: 43264

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVIGGDGYCGWATALYLSNRGYEVGILDSLVRRHWDNELGIETLTPIAPIQQRLQRW
CEEEEEECCCCCHHHEEEEECCCCCEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
QDLTGKSIDLFVGDITNYEFLQKALHKFEPNAIVHFGEQRSAPFSMIDREHAVVTQVNNV
HHCCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHCCCHHHHHHHHHH
VGTLNLLYAMKEDFPDCHLVKLGTMGEYGTPNIDIEEGYITIEHNGRKDTLPYPKQPGSM
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCE
YHLSKVHDSHNIHFACRIWGLRATDLNQGVVYGVLTEETGMDELLINRLDYDGVFGTALN
EEEHHHCCCCCEEEEEEEEEEEECCCCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHHHH
RFCIQAATGHPLTVYGTGGQTRGFLDIRDTVRCVELAIANPAPSGEFRVFNQFTEQFSVG
HHHHEECCCCCEEEEECCCCCCCEEEHHHHHHEEHHEECCCCCCCCCHHHHHHHHHCCCC
DLALMVKKAGNALGLNVEINNLDNPRIEKEEHYFNAKNTKLLDLGLQPHYLSDSLLDSLL
HHEEEEECCCCEEEEEEEECCCCCCCCCCHHHEECCCCCEEEEECCCCCCCCHHHHHHHH
NFAVKYQGRVDQKQILPKVSWHRK
HHHHHHCCCCCHHHHCCCCCCCCC
>Mature Secondary Structure
MKVLVIGGDGYCGWATALYLSNRGYEVGILDSLVRRHWDNELGIETLTPIAPIQQRLQRW
CEEEEEECCCCCHHHEEEEECCCCCEEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
QDLTGKSIDLFVGDITNYEFLQKALHKFEPNAIVHFGEQRSAPFSMIDREHAVVTQVNNV
HHCCCCEEEEEEECCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHHCCCHHHHHHHHHH
VGTLNLLYAMKEDFPDCHLVKLGTMGEYGTPNIDIEEGYITIEHNGRKDTLPYPKQPGSM
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCCCE
YHLSKVHDSHNIHFACRIWGLRATDLNQGVVYGVLTEETGMDELLINRLDYDGVFGTALN
EEEHHHCCCCCEEEEEEEEEEEECCCCCCEEEEEEECCCCHHHHHHHHCCCCCHHHHHHH
RFCIQAATGHPLTVYGTGGQTRGFLDIRDTVRCVELAIANPAPSGEFRVFNQFTEQFSVG
HHHHEECCCCCEEEEECCCCCCCEEEHHHHHHEEHHEECCCCCCCCCHHHHHHHHHCCCC
DLALMVKKAGNALGLNVEINNLDNPRIEKEEHYFNAKNTKLLDLGLQPHYLSDSLLDSLL
HHEEEEECCCCEEEEEEEECCCCCCCCCCHHHEECCCCCEEEEECCCCCCCCHHHHHHHH
NFAVKYQGRVDQKQILPKVSWHRK
HHHHHHCCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA