Click here to switch to the map view.

The map label for this gene is gacS [H]

Identifier: 17229172

GI number: 17229172

Start: 2003284

End: 2004402

Strand: Direct

Name: gacS [H]

Synonym: alr1680

Alternate gene names: 17229172

Gene position: 2003284-2004402 (Clockwise)

Preceding gene: 17229169

Following gene: 17229177

Centisome position: 31.23

GC content: 32.44

Gene sequence:

>1119_bases
ATGAATGCACATATTACAGAAAACCTATTTGCGGCTTTAAACATTGTTGTATTAGAGAGAATCGAGGCTGGATTATTTAA
AATTATTAGTAATTTACCTAGTTGGTTTAGGGATTTTTGTCAGCAGAAATTCAGTTTAGGGATGGAGATTTCCATTCTGC
AAGAAGAATTTTATTTTTTAGAAAACTTTCTGTTTGATGCCGAAGAATTTTGGAGTACTAGTAATTGTAAAAACTTAACT
TCGGGTATCTGGATACAAATAGATCCGCAAGGTAAAGAATATCAATTTGAAGCTCATGCAACTTTTGTCGAAGATAAAAA
AATATTATTAATTGAAGTTTTAGAGGAAAGCTATAGACATAGGCAAAATATTATACAAGAGGCTCGACAACGGCAACTAA
ATTATCAACAATTGATAAAAAATAATCAAAAGCAAGAAATACTAATTCATTGTTTAATCCATGATATTGCGGGACAATTA
AATGCTATGGTTGGGTGTTTATCTTTATTAGAATTTGAAAATTTAACTCCTCAGGGAAGAAATTATTTGGAAGTTTGTCA
ACAACAATCTTTCAATCAAGAAAATCTGATGAGAAAAGTTTTAGATACATTTTCTGCGGAAATGATAGCATTAGAAAATT
TTGTTGTTGATGGTGAGGAAGCGCCAAATATTTTAGGTTGTATAAATGATGTCGTCGAAACCTTAAAACCTAGTTTTACA
TTAAACAATATCAACTTAACGTTAGCTGATGCTATTGATCCTTTAGCAAACTGGAAAGTACAGGGAGATAAATCTCGTTT
AGATAGAGTAATTTTTAATATACTAGAAAATGCTGTCCGCTACAGCCCACCGGAGTCTACAGTAATTATCCGCTTACAAT
CAAAAGAAAATTATATATACGTTTCTGTAGATGATGAAGGCAGTGGAGTACCACTAGAAACGGTTAACACTTTATTTCAA
AAGTTTTCTCAAGGTAAAGATAAAAGTAGTGGAAAATCTGGTTTAGGCCTTTATTTTTGCCGAATTACGGTTGAGCGTTG
GGGGGGTAGTATTGGTTATGCTCCACGTAGTGAAGGTGGTTCTTGCTTTTGGTTTTGTCTACCAAGATTTTTATCTTAA

Upstream 100 bases:

>100_bases
GATTGGCAATACCCACCCTACTTATAATTCACAAATCAAATAGTATTCCTATAGTCATTTCTGTTAAACTGCACTATCAT
GCTGAACTTTCTTGATAATT

Downstream 100 bases:

>100_bases
CCTTGACATTATCGGGTTATAGTTTGTCTCGTTGGTAATGGGTAATAGGTAATAGGTAATTAGTGTTATTTCCCATTACC
AATTACTGATGATCAAGTCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MNAHITENLFAALNIVVLERIEAGLFKIISNLPSWFRDFCQQKFSLGMEISILQEEFYFLENFLFDAEEFWSTSNCKNLT
SGIWIQIDPQGKEYQFEAHATFVEDKKILLIEVLEESYRHRQNIIQEARQRQLNYQQLIKNNQKQEILIHCLIHDIAGQL
NAMVGCLSLLEFENLTPQGRNYLEVCQQQSFNQENLMRKVLDTFSAEMIALENFVVDGEEAPNILGCINDVVETLKPSFT
LNNINLTLADAIDPLANWKVQGDKSRLDRVIFNILENAVRYSPPESTVIIRLQSKENYIYVSVDDEGSGVPLETVNTLFQ
KFSQGKDKSSGKSGLGLYFCRITVERWGGSIGYAPRSEGGSCFWFCLPRFLS

Sequences:

>Translated_372_residues
MNAHITENLFAALNIVVLERIEAGLFKIISNLPSWFRDFCQQKFSLGMEISILQEEFYFLENFLFDAEEFWSTSNCKNLT
SGIWIQIDPQGKEYQFEAHATFVEDKKILLIEVLEESYRHRQNIIQEARQRQLNYQQLIKNNQKQEILIHCLIHDIAGQL
NAMVGCLSLLEFENLTPQGRNYLEVCQQQSFNQENLMRKVLDTFSAEMIALENFVVDGEEAPNILGCINDVVETLKPSFT
LNNINLTLADAIDPLANWKVQGDKSRLDRVIFNILENAVRYSPPESTVIIRLQSKENYIYVSVDDEGSGVPLETVNTLFQ
KFSQGKDKSSGKSGLGLYFCRITVERWGGSIGYAPRSEGGSCFWFCLPRFLS
>Mature_372_residues
MNAHITENLFAALNIVVLERIEAGLFKIISNLPSWFRDFCQQKFSLGMEISILQEEFYFLENFLFDAEEFWSTSNCKNLT
SGIWIQIDPQGKEYQFEAHATFVEDKKILLIEVLEESYRHRQNIIQEARQRQLNYQQLIKNNQKQEILIHCLIHDIAGQL
NAMVGCLSLLEFENLTPQGRNYLEVCQQQSFNQENLMRKVLDTFSAEMIALENFVVDGEEAPNILGCINDVVETLKPSFT
LNNINLTLADAIDPLANWKVQGDKSRLDRVIFNILENAVRYSPPESTVIIRLQSKENYIYVSVDDEGSGVPLETVNTLFQ
KFSQGKDKSSGKSGLGLYFCRITVERWGGSIGYAPRSEGGSCFWFCLPRFLS

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786912, Length=243, Percent_Identity=28.3950617283951, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1789149, Length=220, Percent_Identity=29.5454545454545, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081816, Length=263, Percent_Identity=26.2357414448669, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI48994928, Length=252, Percent_Identity=22.2222222222222, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693157, Length=234, Percent_Identity=24.3589743589744, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42645; Mature: 42645

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAHITENLFAALNIVVLERIEAGLFKIISNLPSWFRDFCQQKFSLGMEISILQEEFYFL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEHHHHHHHHH
ENFLFDAEEFWSTSNCKNLTSGIWIQIDPQGKEYQFEAHATFVEDKKILLIEVLEESYRH
HHHHHCHHHHCCCCCCCCCCCCEEEEECCCCCCEEEEEHEEEECCCCHHHHHHHHHHHHH
RQNIIQEARQRQLNYQQLIKNNQKQEILIHCLIHDIAGQLNAMVGCLSLLEFENLTPQGR
HHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NYLEVCQQQSFNQENLMRKVLDTFSAEMIALENFVVDGEEAPNILGCINDVVETLKPSFT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCEE
LNNINLTLADAIDPLANWKVQGDKSRLDRVIFNILENAVRYSPPESTVIIRLQSKENYIY
EECCEEEEHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
VSVDDEGSGVPLETVNTLFQKFSQGKDKSSGKSGLGLYFCRITVERWGGSIGYAPRSEGG
EEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEHHHCCCCCCCCCCCCCC
SCFWFCLPRFLS
CEEEEEHHHHCC
>Mature Secondary Structure
MNAHITENLFAALNIVVLERIEAGLFKIISNLPSWFRDFCQQKFSLGMEISILQEEFYFL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEHHHHHHHHH
ENFLFDAEEFWSTSNCKNLTSGIWIQIDPQGKEYQFEAHATFVEDKKILLIEVLEESYRH
HHHHHCHHHHCCCCCCCCCCCCEEEEECCCCCCEEEEEHEEEECCCCHHHHHHHHHHHHH
RQNIIQEARQRQLNYQQLIKNNQKQEILIHCLIHDIAGQLNAMVGCLSLLEFENLTPQGR
HHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
NYLEVCQQQSFNQENLMRKVLDTFSAEMIALENFVVDGEEAPNILGCINDVVETLKPSFT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCEE
LNNINLTLADAIDPLANWKVQGDKSRLDRVIFNILENAVRYSPPESTVIIRLQSKENYIY
EECCEEEEHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEE
VSVDDEGSGVPLETVNTLFQKFSQGKDKSSGKSGLGLYFCRITVERWGGSIGYAPRSEGG
EEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEHHHCCCCCCCCCCCCCC
SCFWFCLPRFLS
CEEEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]