Click here to switch to the map view.

The map label for this gene is phoR [H]

Identifier: 17229157

GI number: 17229157

Start: 1986107

End: 1987522

Strand: Direct

Name: phoR [H]

Synonym: alr1665

Alternate gene names: 17229157

Gene position: 1986107-1987522 (Clockwise)

Preceding gene: 17229154

Following gene: 17229158

Centisome position: 30.97

GC content: 41.67

Gene sequence:

>1416_bases
ATGAATGACTCTCTGCATAAGGATTTGTCCGTTTATCAGCTGGCTTTAGGAGTGGAAGCGCCTTCTCAAACAGTGACTCT
CAGTCCGGCGACCTTGCTATCACTGGTGAGAGCGCAAATAGACTTACTGATTGAGCAGCAACTCAGTGCAACTTTATGGA
TTAAGCTGCCACCAGGAAAAGTTTGGTCTACAGAAATAGTCCGCTATCAGTCATCAGTGGATAACTCTGGTCGTATTTAC
AATTGCCAAGTTGGAGAAAGTAAATCAGAGGTGGGAGAAGAAAATTTAACGTCTTTATCTTGTTTGTATGAGCATTATTT
TAATGTGCCATTACTACCAAATAATCACTTACAACGAGAATATTTTTTGGTAGTATTATCGACAAAATTTTGCAGTTTAA
TAGCAGCTTATCGCCCACGGCAAAATAACAAAATTAGTCCCTTGGGGAAGACACAAAATAATAAAAATCAACCCCTATTA
ACGATTACTTCGGTTGAACATCAAGTCATTCAGCGTGTGTTAGATGGAATAAAACAAGAGGTTACCTCTGAATTAAAGCC
AATAGCACCAAGCGATTTGATTTGCCCAACTGCTTGTGAGCCAGCCCTCATTAGCCAGATGCTCACCAAGCAGCTCCAAC
GACAAAATGAAATTAATCGTCGCATTAGTGTTGAACGCATTGCTAAATTGCAGCAGCATAATCAAAAATTGCAAACAAAA
GAGCAATTGAAAGATGAATATTTAACTAATGTGTGTCAAGAACTGCGTACACCCTTGACGCAAATGAAAACTGCGCTTTC
GCTGTTGAATTCGCCCACCCTGAAACCACACCAAAGACAGAGGTATTTGCAAATGTTAAATACCCAGTGCGATCGCCAAA
GTGTTTTGATTGCCGGTCTCATGACTTTGGTAGAACTAGAGCATAATTTAGAAACCACAACTTTAGAAGCTGTCAAACTT
GCAGATATTGTCCCTGGAGTCGTCAGCACTTATCAACCAGTAGCCCAAGAAAAAGGCATCATGCTAGGCTACACCATCCC
TACAGACCTGCCAAGTGTTTTGTGTGTCAATGGTGGATTGCGCCAAATCGTCATTAATCTGCTGCACAACAGCCTCAAGT
TTACAGCTAATGGTGGTCGGGTCTGGGTTAGAGCTAAGGTACAAGGTGAATACGTCATACTAGAAATTCGTGACACAGGT
ATCGGTATTGCCGAAGGCGAAATTCCCAAAATATTCGACTGCTTTTATCGTGTGCGATCGGGGCTAATTGATGAGACCAA
TGGCGCAGGTTTAGGACTAACAATTGTCCAGAGATTGTTATGGCATTGTGGGGGTTCTGTTAATGTGAGAAGTAAGGTTG
ATGAAGGCACTATGGTAATAGTGCAAATGAAGATAGGAACCACCTCGCCAACTTAA

Upstream 100 bases:

>100_bases
GGTGGCTTTTCTCCCTACCAATCATGAGTTAGGCTTTCCCTCTACTTCAAAAAAGTAATTATTTTTTCCTCCCCTGCTTT
TTATTGGTTGTGCTTAACTC

Downstream 100 bases:

>100_bases
ACCAATTCGCAATAGCCTGGGGCAAGGCTAACGCCAACGCAATTCGCTTTTTCCGTGTTGTAACGGGATTTAGACCCCTA
CATTTTGTGAAACCTGAAAA

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MNDSLHKDLSVYQLALGVEAPSQTVTLSPATLLSLVRAQIDLLIEQQLSATLWIKLPPGKVWSTEIVRYQSSVDNSGRIY
NCQVGESKSEVGEENLTSLSCLYEHYFNVPLLPNNHLQREYFLVVLSTKFCSLIAAYRPRQNNKISPLGKTQNNKNQPLL
TITSVEHQVIQRVLDGIKQEVTSELKPIAPSDLICPTACEPALISQMLTKQLQRQNEINRRISVERIAKLQQHNQKLQTK
EQLKDEYLTNVCQELRTPLTQMKTALSLLNSPTLKPHQRQRYLQMLNTQCDRQSVLIAGLMTLVELEHNLETTTLEAVKL
ADIVPGVVSTYQPVAQEKGIMLGYTIPTDLPSVLCVNGGLRQIVINLLHNSLKFTANGGRVWVRAKVQGEYVILEIRDTG
IGIAEGEIPKIFDCFYRVRSGLIDETNGAGLGLTIVQRLLWHCGGSVNVRSKVDEGTMVIVQMKIGTTSPT

Sequences:

>Translated_471_residues
MNDSLHKDLSVYQLALGVEAPSQTVTLSPATLLSLVRAQIDLLIEQQLSATLWIKLPPGKVWSTEIVRYQSSVDNSGRIY
NCQVGESKSEVGEENLTSLSCLYEHYFNVPLLPNNHLQREYFLVVLSTKFCSLIAAYRPRQNNKISPLGKTQNNKNQPLL
TITSVEHQVIQRVLDGIKQEVTSELKPIAPSDLICPTACEPALISQMLTKQLQRQNEINRRISVERIAKLQQHNQKLQTK
EQLKDEYLTNVCQELRTPLTQMKTALSLLNSPTLKPHQRQRYLQMLNTQCDRQSVLIAGLMTLVELEHNLETTTLEAVKL
ADIVPGVVSTYQPVAQEKGIMLGYTIPTDLPSVLCVNGGLRQIVINLLHNSLKFTANGGRVWVRAKVQGEYVILEIRDTG
IGIAEGEIPKIFDCFYRVRSGLIDETNGAGLGLTIVQRLLWHCGGSVNVRSKVDEGTMVIVQMKIGTTSPT
>Mature_471_residues
MNDSLHKDLSVYQLALGVEAPSQTVTLSPATLLSLVRAQIDLLIEQQLSATLWIKLPPGKVWSTEIVRYQSSVDNSGRIY
NCQVGESKSEVGEENLTSLSCLYEHYFNVPLLPNNHLQREYFLVVLSTKFCSLIAAYRPRQNNKISPLGKTQNNKNQPLL
TITSVEHQVIQRVLDGIKQEVTSELKPIAPSDLICPTACEPALISQMLTKQLQRQNEINRRISVERIAKLQQHNQKLQTK
EQLKDEYLTNVCQELRTPLTQMKTALSLLNSPTLKPHQRQRYLQMLNTQCDRQSVLIAGLMTLVELEHNLETTTLEAVKL
ADIVPGVVSTYQPVAQEKGIMLGYTIPTDLPSVLCVNGGLRQIVINLLHNSLKFTANGGRVWVRAKVQGEYVILEIRDTG
IGIAEGEIPKIFDCFYRVRSGLIDETNGAGLGLTIVQRLLWHCGGSVNVRSKVDEGTMVIVQMKIGTTSPT

Specific function: Member of the two-component regulatory system phoP/phoR involved in the alkaline phosphatase genes regulation. PhoR may function as a membrane-associated protein kinase that phosphorylates phoP in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=254, Percent_Identity=28.740157480315, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789149, Length=233, Percent_Identity=30.4721030042918, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786600, Length=220, Percent_Identity=30, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI48994928, Length=234, Percent_Identity=26.0683760683761, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081816, Length=252, Percent_Identity=30.1587301587302, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786783, Length=219, Percent_Identity=26.027397260274, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1790861, Length=241, Percent_Identity=28.6307053941909, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790436, Length=220, Percent_Identity=23.6363636363636, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788393, Length=219, Percent_Identity=25.1141552511416, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786912, Length=347, Percent_Identity=23.0547550432277, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI87082128, Length=208, Percent_Identity=25.4807692307692, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52613; Mature: 52613

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSLHKDLSVYQLALGVEAPSQTVTLSPATLLSLVRAQIDLLIEQQLSATLWIKLPPGK
CCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
VWSTEIVRYQSSVDNSGRIYNCQVGESKSEVGEENLTSLSCLYEHYFNVPLLPNNHLQRE
CCHHHHHHHHHCCCCCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCE
YFLVVLSTKFCSLIAAYRPRQNNKISPLGKTQNNKNQPLLTITSVEHQVIQRVLDGIKQE
EEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHH
VTSELKPIAPSDLICPTACEPALISQMLTKQLQRQNEINRRISVERIAKLQQHNQKLQTK
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQLKDEYLTNVCQELRTPLTQMKTALSLLNSPTLKPHQRQRYLQMLNTQCDRQSVLIAGL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
MTLVELEHNLETTTLEAVKLADIVPGVVSTYQPVAQEKGIMLGYTIPTDLPSVLCVNGGL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHEECCCCH
RQIVINLLHNSLKFTANGGRVWVRAKVQGEYVILEIRDTGIGIAEGEIPKIFDCFYRVRS
HHHHHHHHHCCCEEEECCCEEEEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHC
GLIDETNGAGLGLTIVQRLLWHCGGSVNVRSKVDEGTMVIVQMKIGTTSPT
CCCCCCCCCCCHHHHHHHHHHHCCCCCEECCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MNDSLHKDLSVYQLALGVEAPSQTVTLSPATLLSLVRAQIDLLIEQQLSATLWIKLPPGK
CCCCHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
VWSTEIVRYQSSVDNSGRIYNCQVGESKSEVGEENLTSLSCLYEHYFNVPLLPNNHLQRE
CCHHHHHHHHHCCCCCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCE
YFLVVLSTKFCSLIAAYRPRQNNKISPLGKTQNNKNQPLLTITSVEHQVIQRVLDGIKQE
EEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHH
VTSELKPIAPSDLICPTACEPALISQMLTKQLQRQNEINRRISVERIAKLQQHNQKLQTK
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQLKDEYLTNVCQELRTPLTQMKTALSLLNSPTLKPHQRQRYLQMLNTQCDRQSVLIAGL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
MTLVELEHNLETTTLEAVKLADIVPGVVSTYQPVAQEKGIMLGYTIPTDLPSVLCVNGGL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHEECCCCH
RQIVINLLHNSLKFTANGGRVWVRAKVQGEYVILEIRDTGIGIAEGEIPKIFDCFYRVRS
HHHHHHHHHCCCEEEECCCEEEEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHC
GLIDETNGAGLGLTIVQRLLWHCGGSVNVRSKVDEGTMVIVQMKIGTTSPT
CCCCCCCCCCCHHHHHHHHHHHCCCCCEECCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3142862; 9387221; 9384377 [H]