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The map label for this gene is nuoE [H]

Identifier: 17228246

GI number: 17228246

Start: 872575

End: 873069

Strand: Direct

Name: nuoE [H]

Synonym: alr0751

Alternate gene names: 17228246

Gene position: 872575-873069 (Clockwise)

Preceding gene: 17228245

Following gene: 17228247

Centisome position: 13.6

GC content: 45.86

Gene sequence:

>495_bases
ATGACTACCACAACGCCACCTCATCCCCATCCCAGTGGTGACAAACGCTTGAAGATGCTAGATGCAGCTATCAAGCGTCA
CCAGTATCAGCAAGACGCATTAATTGAAATTCTTCACAAAGCCCAAGAACTGTTTGGTTACTTAGAAAACGACTTATTAC
TTTATATTGCCCATAGCTTGAAGCTACCGCCTAGCCGAGTGTATGGGGTAGCAACTTTCTATCATTTATTTTCCCTAGCG
CCCCAGGGAGTCCATAGTTGTGTGGTGTGTACAGGTACGGCTTGTTATGTGAAAGGCTCATCAGCAATTTTGGCAGATTT
GGAAAAAGCTACTCGTATTCACGCTGGGGAAACTACTGCTGATGGTCAATTATCACTGCTAACGGCACGGTGTTTGGGTG
CTTGTGGGATTGCTCCGGCTGTGGTTTTTGATGGCAAAGTCTTAGGTAATCAAACCCCAGAATCAGTGAATGAGCGCGTG
CAAGGATGGCTATAA

Upstream 100 bases:

>100_bases
ACCTGCATAGTTAGAAATAGGGGTGTAAGGGTATAGGGGTAAATCTTTCTTTTCCTTACACCCTCACACCCTTACACCCA
GTAACGACTTCCAATTTCCC

Downstream 100 bases:

>100_bases
AAAATTCAAAATTCAAAATTCAAAATTCAAAATTAATAGTACTTACGCAAACAGGTTATCTGTTGAGACTGGGTGTAAGG
GGTTCAAGAATTTATACCCT

Product: bidirectional hydrogenase complex protein HoxE

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MTTTTPPHPHPSGDKRLKMLDAAIKRHQYQQDALIEILHKAQELFGYLENDLLLYIAHSLKLPPSRVYGVATFYHLFSLA
PQGVHSCVVCTGTACYVKGSSAILADLEKATRIHAGETTADGQLSLLTARCLGACGIAPAVVFDGKVLGNQTPESVNERV
QGWL

Sequences:

>Translated_164_residues
MTTTTPPHPHPSGDKRLKMLDAAIKRHQYQQDALIEILHKAQELFGYLENDLLLYIAHSLKLPPSRVYGVATFYHLFSLA
PQGVHSCVVCTGTACYVKGSSAILADLEKATRIHAGETTADGQLSLLTARCLGACGIAPAVVFDGKVLGNQTPESVNERV
QGWL
>Mature_163_residues
TTTTPPHPHPSGDKRLKMLDAAIKRHQYQQDALIEILHKAQELFGYLENDLLLYIAHSLKLPPSRVYGVATFYHLFSLAP
QGVHSCVVCTGTACYVKGSSAILADLEKATRIHAGETTADGQLSLLTARCLGACGIAPAVVFDGKVLGNQTPESVNERVQ
GWL

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=157, Percent_Identity=32.484076433121, Blast_Score=91, Evalue=4e-19,
Organism=Escherichia coli, GI1788621, Length=139, Percent_Identity=35.2517985611511, Blast_Score=84, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17561328, Length=130, Percent_Identity=39.2307692307692, Blast_Score=96, Evalue=9e-21,
Organism=Drosophila melanogaster, GI18859877, Length=116, Percent_Identity=38.7931034482759, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21355353, Length=157, Percent_Identity=28.6624203821656, Blast_Score=76, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17767; Mature: 17635

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTTPPHPHPSGDKRLKMLDAAIKRHQYQQDALIEILHKAQELFGYLENDLLLYIAHSL
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KLPPSRVYGVATFYHLFSLAPQGVHSCVVCTGTACYVKGSSAILADLEKATRIHAGETTA
CCCHHHHHHHHHHHHHHHHCCCCHHEEEEECCCEEEEECCHHHHHHHHHHHHHCCCCCCC
DGQLSLLTARCLGACGIAPAVVFDGKVLGNQTPESVNERVQGWL
CCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
TTTTPPHPHPSGDKRLKMLDAAIKRHQYQQDALIEILHKAQELFGYLENDLLLYIAHSL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KLPPSRVYGVATFYHLFSLAPQGVHSCVVCTGTACYVKGSSAILADLEKATRIHAGETTA
CCCHHHHHHHHHHHHHHHHCCCCHHEEEEECCCEEEEECCHHHHHHHHHHHHHCCCCCCC
DGQLSLLTARCLGACGIAPAVVFDGKVLGNQTPESVNERVQGWL
CCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA