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The map label for this gene is narB [H]

Identifier: 17228108

GI number: 17228108

Start: 711707

End: 713947

Strand: Direct

Name: narB [H]

Synonym: alr0612

Alternate gene names: 17228108

Gene position: 711707-713947 (Clockwise)

Preceding gene: 17228107

Following gene: 17228109

Centisome position: 11.1

GC content: 46.9

Gene sequence:

>2241_bases
GTGACTGAATCTACCAAAACCCTATGTCCTTATTGTGGTGTCGGCTGTGGACTAGAAGTAACACCACCCGCCCAACTCAA
CAAAGCCACAAATCGAGATAGCCAAGGAAATCCAACTTGGCGGGTGCGGGGTGATAAAGCCCATCCATCTAGCCAGGGTA
TGGTTTGTGTCAAAGGGGCAACGATCGCCGAATCTTTGGATAAAAATAGATTACATTACCCAATGGTACGGGAATCACTT
GATCAAGAGTTCCGCCGTGTCAGTTGGGATGAAGCTTTTGACCTCATCACCAAGCGTATTCAATCTGTGCGCTTTACCCA
GGGGGCGGAAGCTATATGTATGTATGGTTCCGGTCAATTCCAAACGGAGGATTACTACATAGCCCAAAAACTGATGAAAG
GTTGTTTGGGTAGCAATAATTTTGATGCTAACTCTCGGCTATGTATGTCTAGTGCGGTCTCTGGGTATATTCAAAGTTTT
GGGGCTGATGGGCCTCCCTGCTGCTATGAAGATTTGGAGTTAACTGACTGTGCATTTTTAATTGGGACAAATACCGCCGA
ATGTCACCCGATTGTTTTTAACCGCCTGGAGAAGTACCACAGAAAAAACCATAAGGTAAAAATGATTGTGGTTGATCCTC
GACGCACACCCACAGCCGAAGCAGCTGATTTACATTTGGCGATTAAACCGGGTACAGATATTGACTTGTTAAATGGAATT
GCTCATTTGTTAATGCGTTGGAACATGATAGATGTCGGTTTCATCGATGACTGTACCAGAAACTTTTCGGCTTACGCTGA
GGTAATTCGCCACTATTCGCCGGAGGTAGTAGCTCGTCAATGTGGTATCACCATCGAAGATTTAGAAACCGCCTCCCGTT
ATTGGGGTGAATCGCAGCGTGTGTTGTCCTTATGGTCGATGGGTGTAAATCAATCAAGTGAAGGTACAGCCAAAGTCAGA
ACGATTATTAACCTGCATTTGATGACGGGACAAGTCGGAAAACCAGGGGCTGGGCCTTTCTCTCTCACTGGTCAACCGAA
TGCAATGGGAGGAAGGGAAGCAGGCGGTTTAGCTCATTTATTACCGGGTTATCGATTGGTGAAAAATCCCCAACACCGGG
CAGAATTAGAAGCATTTTGGGGATTCCAGCCAGGGCAAATTTCACCCCATCCTGGTTTAACTGCATGGGACATGATCACT
GGCTTGGAAGACGGTAACGTGGGTTTACTGTGGATTGCGGCTACTAACCCAGCTGTAAGTATGCCAGATTTGGAACGGAC
GAAAAAGGCATTATTGCGATCGCCTTTCACCATCTACCAAGATGCTTACCACCCCACAGAAACTACAGCTTATGCCCATG
TCTTGTTACCAGCAGCCCAGTGGGGTGAAAAAACTGGTATCATGACTAACTCAGAACGTCGAGTTACCCTATGTCAAGCC
TTCCGCCAACCACCACGAGAAGCTAAACCAGATTGGGAAATCTTCGCAGAAGTGGGACGCAGATTAGGTTTTGAGGACAA
GTTCGCCTTTACTAACTCAGCCCAAGTATACGCCGAGTTTACCCAACTGACCAAGGGTCGCCCTTGTGATATGTCTGGTA
TCAGCCATCAACAATTGCAAGCCCAAGGCCCCACTCAATGGCCCCACTCCTCTATAAATATCGAAACACAAAAACCAACT
CAACACCCAACAGTCAACAGTCAACAGTCAACAGTCACCAGTCAACAGTCAAAAAGACTCTACACTGATTTACGTTTTCA
TACCCCTGATGGTCGCGCTCGATTTGGCGCATATTATTCTAAGGGATTAGCAGAACCACCAGACCCTAGTTATCCTTTTG
TGTTGACTACTGGACGATTATACGGACATTGGCACACCCAAACCCGTACTGGTCGCATTGAAAAAATCCGCGCCATGCAC
CCCGAACCATTTATCGAAATTCATCCTCGTGATGCGGCGAAGTTAGGCATTACTGATAATGTGGTGGTGGAAGTGCGATC
GCGTCGAGGTAGCGCCAAGTTTCCCGCCAAGGTGACAAAAGCTATTTCCCCAGGTACAGTTTTTGTGCCTATGCACTGGG
GTAAACTCTGGGCAGATGACGCAGAAGCTAACGCCCTCACCCATTCAGAATCTTGCCCCGATTCACTGCAACCAGAATTA
AAAGCCTGTGCAGTCCAATTAATACCAATCTCCGTAGAAAACACAGCCCAAAATTATCAACTCCAGTCATCTCAGTGGTA
A

Upstream 100 bases:

>100_bases
CAACAGTCAACAGTCATCAGTCTATTTTCTTCCCCCTGCTCACTACCCCACTCTTTTTTTGAATTTTGAATTTTGAATTT
TGAATTCGGAGCGAAGCGAC

Downstream 100 bases:

>100_bases
GCTGTTACGCATTTAAATTGTATATTTCGCACTCAGGTTGTCAAAAGTCAAAAGTCAAAAGTCCAAGCTAGCCTTTGACC
CTGTACTCTTGACTGTGGAC

Product: nitrate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 746; Mature: 745

Protein sequence:

>746_residues
MTESTKTLCPYCGVGCGLEVTPPAQLNKATNRDSQGNPTWRVRGDKAHPSSQGMVCVKGATIAESLDKNRLHYPMVRESL
DQEFRRVSWDEAFDLITKRIQSVRFTQGAEAICMYGSGQFQTEDYYIAQKLMKGCLGSNNFDANSRLCMSSAVSGYIQSF
GADGPPCCYEDLELTDCAFLIGTNTAECHPIVFNRLEKYHRKNHKVKMIVVDPRRTPTAEAADLHLAIKPGTDIDLLNGI
AHLLMRWNMIDVGFIDDCTRNFSAYAEVIRHYSPEVVARQCGITIEDLETASRYWGESQRVLSLWSMGVNQSSEGTAKVR
TIINLHLMTGQVGKPGAGPFSLTGQPNAMGGREAGGLAHLLPGYRLVKNPQHRAELEAFWGFQPGQISPHPGLTAWDMIT
GLEDGNVGLLWIAATNPAVSMPDLERTKKALLRSPFTIYQDAYHPTETTAYAHVLLPAAQWGEKTGIMTNSERRVTLCQA
FRQPPREAKPDWEIFAEVGRRLGFEDKFAFTNSAQVYAEFTQLTKGRPCDMSGISHQQLQAQGPTQWPHSSINIETQKPT
QHPTVNSQQSTVTSQQSKRLYTDLRFHTPDGRARFGAYYSKGLAEPPDPSYPFVLTTGRLYGHWHTQTRTGRIEKIRAMH
PEPFIEIHPRDAAKLGITDNVVVEVRSRRGSAKFPAKVTKAISPGTVFVPMHWGKLWADDAEANALTHSESCPDSLQPEL
KACAVQLIPISVENTAQNYQLQSSQW

Sequences:

>Translated_746_residues
MTESTKTLCPYCGVGCGLEVTPPAQLNKATNRDSQGNPTWRVRGDKAHPSSQGMVCVKGATIAESLDKNRLHYPMVRESL
DQEFRRVSWDEAFDLITKRIQSVRFTQGAEAICMYGSGQFQTEDYYIAQKLMKGCLGSNNFDANSRLCMSSAVSGYIQSF
GADGPPCCYEDLELTDCAFLIGTNTAECHPIVFNRLEKYHRKNHKVKMIVVDPRRTPTAEAADLHLAIKPGTDIDLLNGI
AHLLMRWNMIDVGFIDDCTRNFSAYAEVIRHYSPEVVARQCGITIEDLETASRYWGESQRVLSLWSMGVNQSSEGTAKVR
TIINLHLMTGQVGKPGAGPFSLTGQPNAMGGREAGGLAHLLPGYRLVKNPQHRAELEAFWGFQPGQISPHPGLTAWDMIT
GLEDGNVGLLWIAATNPAVSMPDLERTKKALLRSPFTIYQDAYHPTETTAYAHVLLPAAQWGEKTGIMTNSERRVTLCQA
FRQPPREAKPDWEIFAEVGRRLGFEDKFAFTNSAQVYAEFTQLTKGRPCDMSGISHQQLQAQGPTQWPHSSINIETQKPT
QHPTVNSQQSTVTSQQSKRLYTDLRFHTPDGRARFGAYYSKGLAEPPDPSYPFVLTTGRLYGHWHTQTRTGRIEKIRAMH
PEPFIEIHPRDAAKLGITDNVVVEVRSRRGSAKFPAKVTKAISPGTVFVPMHWGKLWADDAEANALTHSESCPDSLQPEL
KACAVQLIPISVENTAQNYQLQSSQW
>Mature_745_residues
TESTKTLCPYCGVGCGLEVTPPAQLNKATNRDSQGNPTWRVRGDKAHPSSQGMVCVKGATIAESLDKNRLHYPMVRESLD
QEFRRVSWDEAFDLITKRIQSVRFTQGAEAICMYGSGQFQTEDYYIAQKLMKGCLGSNNFDANSRLCMSSAVSGYIQSFG
ADGPPCCYEDLELTDCAFLIGTNTAECHPIVFNRLEKYHRKNHKVKMIVVDPRRTPTAEAADLHLAIKPGTDIDLLNGIA
HLLMRWNMIDVGFIDDCTRNFSAYAEVIRHYSPEVVARQCGITIEDLETASRYWGESQRVLSLWSMGVNQSSEGTAKVRT
IINLHLMTGQVGKPGAGPFSLTGQPNAMGGREAGGLAHLLPGYRLVKNPQHRAELEAFWGFQPGQISPHPGLTAWDMITG
LEDGNVGLLWIAATNPAVSMPDLERTKKALLRSPFTIYQDAYHPTETTAYAHVLLPAAQWGEKTGIMTNSERRVTLCQAF
RQPPREAKPDWEIFAEVGRRLGFEDKFAFTNSAQVYAEFTQLTKGRPCDMSGISHQQLQAQGPTQWPHSSINIETQKPTQ
HPTVNSQQSTVTSQQSKRLYTDLRFHTPDGRARFGAYYSKGLAEPPDPSYPFVLTTGRLYGHWHTQTRTGRIEKIRAMHP
EPFIEIHPRDAAKLGITDNVVVEVRSRRGSAKFPAKVTKAISPGTVFVPMHWGKLWADDAEANALTHSESCPDSLQPELK
ACAVQLIPISVENTAQNYQLQSSQW

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=746, Percent_Identity=29.088471849866, Blast_Score=344, Evalue=1e-95,
Organism=Escherichia coli, GI1788534, Length=814, Percent_Identity=29.6068796068796, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI3868719, Length=628, Percent_Identity=24.0445859872611, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI3868720, Length=629, Percent_Identity=23.5294117647059, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI87081797, Length=737, Percent_Identity=24.0162822252374, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1787778, Length=415, Percent_Identity=26.0240963855422, Blast_Score=119, Evalue=1e-27,
Organism=Escherichia coli, GI1787870, Length=711, Percent_Identity=25.5977496483826, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI171474008, Length=740, Percent_Identity=24.0540540540541, Blast_Score=116, Evalue=5e-27,
Organism=Escherichia coli, GI145693196, Length=693, Percent_Identity=21.7893217893218, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI87081994, Length=707, Percent_Identity=21.9236209335219, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 83014; Mature: 82883

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESTKTLCPYCGVGCGLEVTPPAQLNKATNRDSQGNPTWRVRGDKAHPSSQGMVCVKGA
CCCCCCCCCCCCCCCCCCEECCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEECC
TIAESLDKNRLHYPMVRESLDQEFRRVSWDEAFDLITKRIQSVRFTQGAEAICMYGSGQF
HHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
QTEDYYIAQKLMKGCLGSNNFDANSRLCMSSAVSGYIQSFGADGPPCCYEDLELTDCAFL
CCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
IGTNTAECHPIVFNRLEKYHRKNHKVKMIVVDPRRTPTAEAADLHLAIKPGTDIDLLNGI
EECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHH
AHLLMRWNMIDVGFIDDCTRNFSAYAEVIRHYSPEVVARQCGITIEDLETASRYWGESQR
HHHHHHCCCEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCHHH
VLSLWSMGVNQSSEGTAKVRTIINLHLMTGQVGKPGAGPFSLTGQPNAMGGREAGGLAHL
HHHHHHHCCCCCCCCHHEEEEEEEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCHHHH
LPGYRLVKNPQHRAELEAFWGFQPGQISPHPGLTAWDMITGLEDGNVGLLWIAATNPAVS
CCCCHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEEECCCCCC
MPDLERTKKALLRSPFTIYQDAYHPTETTAYAHVLLPAAQWGEKTGIMTNSERRVTLCQA
CCCHHHHHHHHHHCCCHHHHCCCCCCCCCEEEEEEECHHHCCCCCCCEECCCCHHHHHHH
FRQPPREAKPDWEIFAEVGRRLGFEDKFAFTNSAQVYAEFTQLTKGRPCDMSGISHQQLQ
HCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHH
AQGPTQWPHSSINIETQKPTQHPTVNSQQSTVTSQQSKRLYTDLRFHTPDGRARFGAYYS
CCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHH
KGLAEPPDPSYPFVLTTGRLYGHWHTQTRTGRIEKIRAMHPEPFIEIHPRDAAKLGITDN
CCCCCCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHCCCCCCEEECCCCCEECCCCCC
VVVEVRSRRGSAKFPAKVTKAISPGTVFVPMHWGKLWADDAEANALTHSESCPDSLQPEL
EEEEEECCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHCCHHH
KACAVQLIPISVENTAQNYQLQSSQW
HHCEEEEEEEEECCCCCCCEECCCCC
>Mature Secondary Structure 
TESTKTLCPYCGVGCGLEVTPPAQLNKATNRDSQGNPTWRVRGDKAHPSSQGMVCVKGA
CCCCCCCCCCCCCCCCCEECCCHHHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEEECC
TIAESLDKNRLHYPMVRESLDQEFRRVSWDEAFDLITKRIQSVRFTQGAEAICMYGSGQF
HHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCC
QTEDYYIAQKLMKGCLGSNNFDANSRLCMSSAVSGYIQSFGADGPPCCYEDLELTDCAFL
CCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEE
IGTNTAECHPIVFNRLEKYHRKNHKVKMIVVDPRRTPTAEAADLHLAIKPGTDIDLLNGI
EECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHH
AHLLMRWNMIDVGFIDDCTRNFSAYAEVIRHYSPEVVARQCGITIEDLETASRYWGESQR
HHHHHHCCCEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCCHHH
VLSLWSMGVNQSSEGTAKVRTIINLHLMTGQVGKPGAGPFSLTGQPNAMGGREAGGLAHL
HHHHHHHCCCCCCCCHHEEEEEEEEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCHHHH
LPGYRLVKNPQHRAELEAFWGFQPGQISPHPGLTAWDMITGLEDGNVGLLWIAATNPAVS
CCCCHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEEECCCCCC
MPDLERTKKALLRSPFTIYQDAYHPTETTAYAHVLLPAAQWGEKTGIMTNSERRVTLCQA
CCCHHHHHHHHHHCCCHHHHCCCCCCCCCEEEEEEECHHHCCCCCCCEECCCCHHHHHHH
FRQPPREAKPDWEIFAEVGRRLGFEDKFAFTNSAQVYAEFTQLTKGRPCDMSGISHQQLQ
HCCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHH
AQGPTQWPHSSINIETQKPTQHPTVNSQQSTVTSQQSKRLYTDLRFHTPDGRARFGAYYS
CCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHH
KGLAEPPDPSYPFVLTTGRLYGHWHTQTRTGRIEKIRAMHPEPFIEIHPRDAAKLGITDN
CCCCCCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHCCCCCCEEECCCCCEECCCCCC
VVVEVRSRRGSAKFPAKVTKAISPGTVFVPMHWGKLWADDAEANALTHSESCPDSLQPEL
EEEEEECCCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHCCHHH
KACAVQLIPISVENTAQNYQLQSSQW
HHCEEEEEEEEECCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]