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The map label for this gene is cobA [H]

Identifier: 17228016

GI number: 17228016

Start: 611773

End: 612555

Strand: Direct

Name: cobA [H]

Synonym: alr0520

Alternate gene names: 17228016

Gene position: 611773-612555 (Clockwise)

Preceding gene: 17228015

Following gene: 17228018

Centisome position: 9.54

GC content: 46.74

Gene sequence:

>783_bases
ATGAACCGCACAGACAATCAGGGGCAAAAGGGTTTGGGTAAGGTCTATTTAGTAGGTGCGGGGCCAGGCGATCCAGGACT
GATGACGGTGAAGGGCAAAGGTATATTAGAATGTGCCGATGTGGTCATATATGATGCTTTAGTCAGTCCAGCAATTTTGG
CAATGATTAATCCCCAAGCAGAGCAGATTAATGCTGGTAAGCGCATGGGGCGACATTCTCTATTGCAGGACGAAACAACT
CAACTACTAATTGAACAAGCCCAAAATCACGCTGTTGTTGTGCGGTTAAAAGGTGGTGACCCCTTTATTTTCGGTCGTGG
CGGGGAAGAGATGGCTGATTTAATTGCGGCTGGGGTTCCGGTGGAGGTTGTGCCAGGGATTACATCAGGAATTGCAGCGC
CAGCCTATGCAGGGATTCCCCTGACTCATCGCTTATATAGTTCATCTGTAACTTTTGTAACTGGGCATGAGTCGGCAGGT
AAGTATCGACCGAAAGTCAATTGGCAAGCGATCGCCCACGGTTCAGAAACGATTGTCATTTATATGGGTATCCACAATCT
ACCTTATATTGTGGAACAGTTGAAAATGGCTGGTTTGAGTTTAGCAACACCAATCGCTCTAGTGCGCTGGGGTACACGTC
CCGAACAGGAAGAATTAATTGGCGAATTAGGGACAATTGTAGAACTAGTGGAGAAAACAGGATTTGAAGCGCCAGCGATC
GCAGTCATTGGCTCTGTAGTCAATATGCACAGTATTTTATCTGTAGCAGCCCGTGTTAATTGA

Upstream 100 bases:

>100_bases
AAGCGCTAAAATTACAATTTCCTGGGGTAAGTTTATACTTGGCTGAACCTTTGGGAGTTAGTGGAGAATTAGCCGATTTA
ATTGGGGATTTGATTTACAC

Downstream 100 bases:

>100_bases
CTTGCTAATGTATCTACATCTAAGAAGGTAATGGGTAATTGGTAATAGTTGATATTACGTAATGTGTTTAAAAATTACTT
TTAGCAAATCACCTTGATTA

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MNRTDNQGQKGLGKVYLVGAGPGDPGLMTVKGKGILECADVVIYDALVSPAILAMINPQAEQINAGKRMGRHSLLQDETT
QLLIEQAQNHAVVVRLKGGDPFIFGRGGEEMADLIAAGVPVEVVPGITSGIAAPAYAGIPLTHRLYSSSVTFVTGHESAG
KYRPKVNWQAIAHGSETIVIYMGIHNLPYIVEQLKMAGLSLATPIALVRWGTRPEQEELIGELGTIVELVEKTGFEAPAI
AVIGSVVNMHSILSVAARVN

Sequences:

>Translated_260_residues
MNRTDNQGQKGLGKVYLVGAGPGDPGLMTVKGKGILECADVVIYDALVSPAILAMINPQAEQINAGKRMGRHSLLQDETT
QLLIEQAQNHAVVVRLKGGDPFIFGRGGEEMADLIAAGVPVEVVPGITSGIAAPAYAGIPLTHRLYSSSVTFVTGHESAG
KYRPKVNWQAIAHGSETIVIYMGIHNLPYIVEQLKMAGLSLATPIALVRWGTRPEQEELIGELGTIVELVEKTGFEAPAI
AVIGSVVNMHSILSVAARVN
>Mature_260_residues
MNRTDNQGQKGLGKVYLVGAGPGDPGLMTVKGKGILECADVVIYDALVSPAILAMINPQAEQINAGKRMGRHSLLQDETT
QLLIEQAQNHAVVVRLKGGDPFIFGRGGEEMADLIAAGVPVEVVPGITSGIAAPAYAGIPLTHRLYSSSVTFVTGHESAG
KYRPKVNWQAIAHGSETIVIYMGIHNLPYIVEQLKMAGLSLATPIALVRWGTRPEQEELIGELGTIVELVEKTGFEAPAI
AVIGSVVNMHSILSVAARVN

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=242, Percent_Identity=44.2148760330579, Blast_Score=202, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6322922, Length=243, Percent_Identity=35.8024691358025, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 27534; Mature: 27534

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTDNQGQKGLGKVYLVGAGPGDPGLMTVKGKGILECADVVIYDALVSPAILAMINPQA
CCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHEEECCCH
EQINAGKRMGRHSLLQDETTQLLIEQAQNHAVVVRLKGGDPFIFGRGGEEMADLIAAGVP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCHHHHHHHHCCCC
VEVVPGITSGIAAPAYAGIPLTHRLYSSSVTFVTGHESAGKYRPKVNWQAIAHGSETIVI
EEECCCCHHCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEECCCCEEEE
YMGIHNLPYIVEQLKMAGLSLATPIALVRWGTRPEQEELIGELGTIVELVEKTGFEAPAI
EEECCCHHHHHHHHHHCCCCHHCHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHH
AVIGSVVNMHSILSVAARVN
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRTDNQGQKGLGKVYLVGAGPGDPGLMTVKGKGILECADVVIYDALVSPAILAMINPQA
CCCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHEEECCCH
EQINAGKRMGRHSLLQDETTQLLIEQAQNHAVVVRLKGGDPFIFGRGGEEMADLIAAGVP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEECCCCHHHHHHHHCCCC
VEVVPGITSGIAAPAYAGIPLTHRLYSSSVTFVTGHESAGKYRPKVNWQAIAHGSETIVI
EEECCCCHHCCCCCCCCCCCHHHHHHCCCEEEEECCCCCCCCCCCCCEEEEECCCCEEEE
YMGIHNLPYIVEQLKMAGLSLATPIALVRWGTRPEQEELIGELGTIVELVEKTGFEAPAI
EEECCCHHHHHHHHHHCCCCHHCHHHHEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHH
AVIGSVVNMHSILSVAARVN
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]