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The map label for this gene is thrS [H]

Identifier: 17227831

GI number: 17227831

Start: 385671

End: 387518

Strand: Direct

Name: thrS [H]

Synonym: alr0335

Alternate gene names: 17227831

Gene position: 385671-387518 (Clockwise)

Preceding gene: 17227827

Following gene: 17227832

Centisome position: 6.01

GC content: 45.08

Gene sequence:

>1848_bases
ATGGTTCAGCAGCTAATGTCGCCTAATCAAGACCCCAACAACCAGCCAGAACAACCAGAAAAAATCTATTTACCGCGTAC
CAGCGAATCAGAAACATTGAAAAAGATTCGCCATACGGCTTCCCATGTAATGGCAATGGCAGTACAGAAGCTGTTCCCTA
AGGCGCAAGTGACAATTGGCCCCTGGATTGAGAATGGGTTTTATTACGACTTTGATAGTCCAGAGCCGTTTACTGATAAG
GATCTCAAAGCCATTCAAAAAGAGATGGTGAAGATTATTAATCGTAAATTGCCAGTGGTGCGGGAAGAAGTGAGCCGAGA
AGAAGCGGAACGGCGCATTCAGGAAATCAAGGAACCTTATAAATTAGAAATTCTGGCAGATATTAAATCAGAACCGATCA
CAATTTACCACCTGGGAAATGAATGGTGGGATTTGTGCGCCGGGCCTCATGTGGAAAATACCAAAGATTTAAATCCCAAA
GCAATTGAATTAGAAAGTGTGGCGGGTGCTTATTGGCGGGGAGATGAAACCAAAGCCCAATTACAACGAATCTATGCTAC
GGCTTGGGAAACCCCAGAACAACTAGCCGAATACAAGCGCCGCAAAGAAGAAGCCCTACGCCGTGACCACCGTAAACTGG
GTAAAGAACTAGGATTATTTATCTTTTCGGAACAAGTGGGGCCTGGTTTACCTTTGTGGACACCAAAAGGCACTTTGTTA
CGCAGCACCTTAGAACAATTTCTCCAGCAGGAACAGCTCAAGCGTGGTTATTTGCCTGTAGTTACCCCCCACATTGCCAG
AGTAGATTTATTTAAGACTTCAGGACACTGGCAAAAATATAAGGAAGATATGTTTCCCCTGATGGCTGAGGATGAAGCAG
CCGCAGCAATTGAGCAAGGCTTTGTCCTCAAGCCAATGAATTGCCCCTTCCACATCCAAATATATAAGAGTGAATTGCGT
TCTTATCGGGAACTACCAATGCGCTTGGCTGAGTTTGGGACTGTTTACCGTTACGAACAGTCAGGGGAATTAGGTGGTTT
AACCAGAGTGCGCGGTTTTACTGTTGATGATTCTCACTTATTCGTGACCCCAGAACAGTTAGACAACGAATTCCTGAATG
TAGTGGATTTGATTTTGTCAGTGTTCAAAAGTCTGCAATTGAAAAACTTTAAAGCTAGACTCAGTTTCCGTGACCCAGCT
AGCGATAAGTACATCGGTTCTGATGAAGTTTGGAACAAAGCCGAAGGTGCAATCCGTCGTGCGGTGGAACAGTTGGGGAT
GGAACACTTTGAAGGGATTGGGGAAGCGGCATTTTATGGCCCGAAGTTAGACTTTATTTTCAGCGATGCTTTAGAAAGAG
AGTGGCAATTAGGAACTGTACAGGTAGATTACAACTTACCAGAACGGTTTGATTTGGAATACGTAGCGGAAGATGGAACT
CGCAAACGTCCCGTGATGATTCACCGTGCGCCTTTCGGTTCCTTAGAACGGTTGATTGGGATTTTAATTGAAGAGTATGC
GGGAGATTTCCCTCTATGGTTAGCACCGGTGCAAGCTAGATTGCTACCTGTAGGAGAAGCCCAGCTAGACTTTACCAAAG
ATGTAGTAGCAAAATTGCGATCGCTTGGTATTCGTGCTGAAGTTGATACCAGTGGCGATCGCTTAGGTAAACTCATCCGC
AATGCAGAGAAAGATAAAATACCAGTGATGGCCGTAGTAGGAGCCAAGGAAGTAGAAACCAACTCCTTAAGTATCCGTAC
CCGCGCTTCTGGAGAGTTGGGAGCTATACCTGTGGATGAAGTGGTGCAGAAGATGAAGGATGCGATCGCTAACTACGAAA
ACTTCTAG

Upstream 100 bases:

>100_bases
GACGAGGGAAAGCTTACACAGCAGGAACATAAGCTCATGTTATATTTGGGTAGATTTTGAAAACTTCAATTGATCATTTA
CTCTTGCAGTCACTTCGCTA

Downstream 100 bases:

>100_bases
TAATTACTTAGCTGGGTATGACCCAGCTAGAAAAACTCAATCTATAAAGTCCCATATCTTTTGCCGCAATTACTAGATGA
GATTTAGTGTATCTAAATTG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MVQQLMSPNQDPNNQPEQPEKIYLPRTSESETLKKIRHTASHVMAMAVQKLFPKAQVTIGPWIENGFYYDFDSPEPFTDK
DLKAIQKEMVKIINRKLPVVREEVSREEAERRIQEIKEPYKLEILADIKSEPITIYHLGNEWWDLCAGPHVENTKDLNPK
AIELESVAGAYWRGDETKAQLQRIYATAWETPEQLAEYKRRKEEALRRDHRKLGKELGLFIFSEQVGPGLPLWTPKGTLL
RSTLEQFLQQEQLKRGYLPVVTPHIARVDLFKTSGHWQKYKEDMFPLMAEDEAAAAIEQGFVLKPMNCPFHIQIYKSELR
SYRELPMRLAEFGTVYRYEQSGELGGLTRVRGFTVDDSHLFVTPEQLDNEFLNVVDLILSVFKSLQLKNFKARLSFRDPA
SDKYIGSDEVWNKAEGAIRRAVEQLGMEHFEGIGEAAFYGPKLDFIFSDALEREWQLGTVQVDYNLPERFDLEYVAEDGT
RKRPVMIHRAPFGSLERLIGILIEEYAGDFPLWLAPVQARLLPVGEAQLDFTKDVVAKLRSLGIRAEVDTSGDRLGKLIR
NAEKDKIPVMAVVGAKEVETNSLSIRTRASGELGAIPVDEVVQKMKDAIANYENF

Sequences:

>Translated_615_residues
MVQQLMSPNQDPNNQPEQPEKIYLPRTSESETLKKIRHTASHVMAMAVQKLFPKAQVTIGPWIENGFYYDFDSPEPFTDK
DLKAIQKEMVKIINRKLPVVREEVSREEAERRIQEIKEPYKLEILADIKSEPITIYHLGNEWWDLCAGPHVENTKDLNPK
AIELESVAGAYWRGDETKAQLQRIYATAWETPEQLAEYKRRKEEALRRDHRKLGKELGLFIFSEQVGPGLPLWTPKGTLL
RSTLEQFLQQEQLKRGYLPVVTPHIARVDLFKTSGHWQKYKEDMFPLMAEDEAAAAIEQGFVLKPMNCPFHIQIYKSELR
SYRELPMRLAEFGTVYRYEQSGELGGLTRVRGFTVDDSHLFVTPEQLDNEFLNVVDLILSVFKSLQLKNFKARLSFRDPA
SDKYIGSDEVWNKAEGAIRRAVEQLGMEHFEGIGEAAFYGPKLDFIFSDALEREWQLGTVQVDYNLPERFDLEYVAEDGT
RKRPVMIHRAPFGSLERLIGILIEEYAGDFPLWLAPVQARLLPVGEAQLDFTKDVVAKLRSLGIRAEVDTSGDRLGKLIR
NAEKDKIPVMAVVGAKEVETNSLSIRTRASGELGAIPVDEVVQKMKDAIANYENF
>Mature_615_residues
MVQQLMSPNQDPNNQPEQPEKIYLPRTSESETLKKIRHTASHVMAMAVQKLFPKAQVTIGPWIENGFYYDFDSPEPFTDK
DLKAIQKEMVKIINRKLPVVREEVSREEAERRIQEIKEPYKLEILADIKSEPITIYHLGNEWWDLCAGPHVENTKDLNPK
AIELESVAGAYWRGDETKAQLQRIYATAWETPEQLAEYKRRKEEALRRDHRKLGKELGLFIFSEQVGPGLPLWTPKGTLL
RSTLEQFLQQEQLKRGYLPVVTPHIARVDLFKTSGHWQKYKEDMFPLMAEDEAAAAIEQGFVLKPMNCPFHIQIYKSELR
SYRELPMRLAEFGTVYRYEQSGELGGLTRVRGFTVDDSHLFVTPEQLDNEFLNVVDLILSVFKSLQLKNFKARLSFRDPA
SDKYIGSDEVWNKAEGAIRRAVEQLGMEHFEGIGEAAFYGPKLDFIFSDALEREWQLGTVQVDYNLPERFDLEYVAEDGT
RKRPVMIHRAPFGSLERLIGILIEEYAGDFPLWLAPVQARLLPVGEAQLDFTKDVVAKLRSLGIRAEVDTSGDRLGKLIR
NAEKDKIPVMAVVGAKEVETNSLSIRTRASGELGAIPVDEVVQKMKDAIANYENF

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=572, Percent_Identity=37.7622377622378, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI38570111, Length=574, Percent_Identity=37.2822299651568, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI20070344, Length=582, Percent_Identity=36.254295532646, Blast_Score=361, Evalue=1e-100,
Organism=Escherichia coli, GI1788013, Length=600, Percent_Identity=39.5, Blast_Score=470, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI71984184, Length=589, Percent_Identity=35.4838709677419, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71984192, Length=589, Percent_Identity=35.4838709677419, Blast_Score=387, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6322112, Length=598, Percent_Identity=36.6220735785953, Blast_Score=378, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6322654, Length=393, Percent_Identity=36.1323155216285, Blast_Score=257, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24583842, Length=585, Percent_Identity=39.3162393162393, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI28574141, Length=585, Percent_Identity=39.3162393162393, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24583839, Length=585, Percent_Identity=39.3162393162393, Blast_Score=421, Evalue=1e-118,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 70471; Mature: 70471

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQQLMSPNQDPNNQPEQPEKIYLPRTSESETLKKIRHTASHVMAMAVQKLFPKAQVTIG
CCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
PWIENGFYYDFDSPEPFTDKDLKAIQKEMVKIINRKLPVVREEVSREEAERRIQEIKEPY
CEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
KLEILADIKSEPITIYHLGNEWWDLCAGPHVENTKDLNPKAIELESVAGAYWRGDETKAQ
EEEEEEECCCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCEEEHHHHCCCCCCCCCHHHH
LQRIYATAWETPEQLAEYKRRKEEALRRDHRKLGKELGLFIFSEQVGPGLPLWTPKGTLL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHH
RSTLEQFLQQEQLKRGYLPVVTPHIARVDLFKTSGHWQKYKEDMFPLMAEDEAAAAIEQG
HHHHHHHHHHHHHHCCCCCCCCCCHHEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHCC
FVLKPMNCPFHIQIYKSELRSYRELPMRLAEFGTVYRYEQSGELGGLTRVRGFTVDDSHL
CEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHCHHEEECCCCCCCCCCEEEEEEECCCEE
FVTPEQLDNEFLNVVDLILSVFKSLQLKNFKARLSFRDPASDKYIGSDEVWNKAEGAIRR
EECHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCCHHHHHHHHHHHHH
AVEQLGMEHFEGIGEAAFYGPKLDFIFSDALEREWQLGTVQVDYNLPERFDLEYVAEDGT
HHHHHCHHHHHCCCCHHHCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEECCCC
RKRPVMIHRAPFGSLERLIGILIEEYAGDFPLWLAPVQARLLPVGEAQLDFTKDVVAKLR
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHEEECCCCHHHHHHHHHHHHH
SLGIRAEVDTSGDRLGKLIRNAEKDKIPVMAVVGAKEVETNSLSIRTRASGELGAIPVDE
HCCCEEEECCCHHHHHHHHHCCCCCCCCEEEEECCCEECCCCEEEEEECCCCCCCCCHHH
VVQKMKDAIANYENF
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MVQQLMSPNQDPNNQPEQPEKIYLPRTSESETLKKIRHTASHVMAMAVQKLFPKAQVTIG
CCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
PWIENGFYYDFDSPEPFTDKDLKAIQKEMVKIINRKLPVVREEVSREEAERRIQEIKEPY
CEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
KLEILADIKSEPITIYHLGNEWWDLCAGPHVENTKDLNPKAIELESVAGAYWRGDETKAQ
EEEEEEECCCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCEEEHHHHCCCCCCCCCHHHH
LQRIYATAWETPEQLAEYKRRKEEALRRDHRKLGKELGLFIFSEQVGPGLPLWTPKGTLL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHH
RSTLEQFLQQEQLKRGYLPVVTPHIARVDLFKTSGHWQKYKEDMFPLMAEDEAAAAIEQG
HHHHHHHHHHHHHHCCCCCCCCCCHHEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHCC
FVLKPMNCPFHIQIYKSELRSYRELPMRLAEFGTVYRYEQSGELGGLTRVRGFTVDDSHL
CEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHCHHEEECCCCCCCCCCEEEEEEECCCEE
FVTPEQLDNEFLNVVDLILSVFKSLQLKNFKARLSFRDPASDKYIGSDEVWNKAEGAIRR
EECHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCCCCCHHHHHHHHHHHHH
AVEQLGMEHFEGIGEAAFYGPKLDFIFSDALEREWQLGTVQVDYNLPERFDLEYVAEDGT
HHHHHCHHHHHCCCCHHHCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEECCCC
RKRPVMIHRAPFGSLERLIGILIEEYAGDFPLWLAPVQARLLPVGEAQLDFTKDVVAKLR
CCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHEEECCCCHHHHHHHHHHHHH
SLGIRAEVDTSGDRLGKLIRNAEKDKIPVMAVVGAKEVETNSLSIRTRASGELGAIPVDE
HCCCEEEECCCHHHHHHHHHCCCCCCCCEEEEECCCEECCCCEEEEEECCCCCCCCCHHH
VVQKMKDAIANYENF
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]