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The map label for this gene is potD [H]

Identifier: 17227795

GI number: 17227795

Start: 339569

End: 340735

Strand: Direct

Name: potD [H]

Synonym: alr0299

Alternate gene names: 17227795

Gene position: 339569-340735 (Clockwise)

Preceding gene: 17227793

Following gene: 17227796

Centisome position: 5.29

GC content: 40.02

Gene sequence:

>1167_bases
ATGTATATTCCTAAATCTATGGATAGAAGGACTTTTTTAGTGAGCCTGGGTGGGCTGACTTTATCACAACTACTCGTAGG
CTGTACGGGGAATAACCAGACACAACTAAACGTTCAGTTATTAAAAGGTTCTATCCCTGGTCAAGTAGTTAATAAATTTC
GCCAAAGTCTAAAGCAACAGGCGCAGTTAAAGTTTGCACCAGTTGAACAACTAGAAGAGTTATATAAACGATTGGTAAAC
TGGCAGCATAACCCAAAAGCTCATGATGAGCAAGAATGGACGCGCTTCATCCCATTTAGGCAATCTCAAACTTTTCCCGT
GGCTGATTTGGTGACATTAGGAGATTATTGGTTAACCACAGCAATTGAGAGAAAACTGATTCAAGCCATAGAAACAGAGC
AATTAAAGCAATGGTCTGGCTTAGATTCAAGATGGCGAGAATTGGTAACGCGTAATGAGCAAGGTGTTCCAGACCCACAA
GGAAAAGTTTGGGCTGCGCCTTATCGCTGGGGTAGTACGGTAATTGTTTATGATCGTGAGAAGTTTAAAAAATTGGGATG
GATACCCCAAGATTGGAGTGATTTGTGGCGTAGTGAATTGCGATCGCGTATTTCTGTACTAGATCAACCAAGAGAAATTA
TAGGTTTAGTCTTAAAGAAACTAGGAAAATCATACAACACAGAAAACCTAGCAACAGTTCCAGATTTAGAAAAAGAACTG
CAAACCTTCAACCAGCAGGTAAAAATCTACAGTTCCAATACCTATTTAGAACCTCTGATTCTTGGAGATACTTGGCTAGC
AGTTGGTTGGTCAAGTGATTTAATCCCAGTTTTGGCACGTTATCCCCAACTTAGCGTTGTGATTCCCCGTTCCGGAACCT
CTATCTGGGCAGACTTATGGGTAAGCCCTAAAGAAACCGCTAAGGATGGTCTATCATCCCAATGGATTGATTTTTGTTTA
CAACCCAATATTGCTAGGCAAATTGCTTTACTCACTAAAACTAATTCCCCAGTTGCCACTAATATTGGTACTGCTGATAT
TCAGGAATCATTAGGTGGTTTATTCCTAAATAGTCAAGAAGTATTTGCTAAAGGTGAATTTTTGCTTCCCCTATCGCCAG
AAGCGACAAAACAATATGAAGCTTTGTTTGCCAAAATCAAAGGTTAA

Upstream 100 bases:

>100_bases
AGTTGAGTAAATTAATAATTCGCTTGCATTTTCTCTAATTATTCAAAATACTAGCTGCTTCAAACCTTCAATCTTTGTTA
TCTCTGTTTAATTTATATAT

Downstream 100 bases:

>100_bases
TTTGTCATTGGTCATTGGTCATCAGTAAACTTCCCCCACCCTATATTCCCCGCTAAACCACAATTATCTGATAATGAACT
GAAGAGTGCATACAAGAGAT

Product: periplasmic polyamine-binding protein of ABC transporter

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MYIPKSMDRRTFLVSLGGLTLSQLLVGCTGNNQTQLNVQLLKGSIPGQVVNKFRQSLKQQAQLKFAPVEQLEELYKRLVN
WQHNPKAHDEQEWTRFIPFRQSQTFPVADLVTLGDYWLTTAIERKLIQAIETEQLKQWSGLDSRWRELVTRNEQGVPDPQ
GKVWAAPYRWGSTVIVYDREKFKKLGWIPQDWSDLWRSELRSRISVLDQPREIIGLVLKKLGKSYNTENLATVPDLEKEL
QTFNQQVKIYSSNTYLEPLILGDTWLAVGWSSDLIPVLARYPQLSVVIPRSGTSIWADLWVSPKETAKDGLSSQWIDFCL
QPNIARQIALLTKTNSPVATNIGTADIQESLGGLFLNSQEVFAKGEFLLPLSPEATKQYEALFAKIKG

Sequences:

>Translated_388_residues
MYIPKSMDRRTFLVSLGGLTLSQLLVGCTGNNQTQLNVQLLKGSIPGQVVNKFRQSLKQQAQLKFAPVEQLEELYKRLVN
WQHNPKAHDEQEWTRFIPFRQSQTFPVADLVTLGDYWLTTAIERKLIQAIETEQLKQWSGLDSRWRELVTRNEQGVPDPQ
GKVWAAPYRWGSTVIVYDREKFKKLGWIPQDWSDLWRSELRSRISVLDQPREIIGLVLKKLGKSYNTENLATVPDLEKEL
QTFNQQVKIYSSNTYLEPLILGDTWLAVGWSSDLIPVLARYPQLSVVIPRSGTSIWADLWVSPKETAKDGLSSQWIDFCL
QPNIARQIALLTKTNSPVATNIGTADIQESLGGLFLNSQEVFAKGEFLLPLSPEATKQYEALFAKIKG
>Mature_388_residues
MYIPKSMDRRTFLVSLGGLTLSQLLVGCTGNNQTQLNVQLLKGSIPGQVVNKFRQSLKQQAQLKFAPVEQLEELYKRLVN
WQHNPKAHDEQEWTRFIPFRQSQTFPVADLVTLGDYWLTTAIERKLIQAIETEQLKQWSGLDSRWRELVTRNEQGVPDPQ
GKVWAAPYRWGSTVIVYDREKFKKLGWIPQDWSDLWRSELRSRISVLDQPREIIGLVLKKLGKSYNTENLATVPDLEKEL
QTFNQQVKIYSSNTYLEPLILGDTWLAVGWSSDLIPVLARYPQLSVVIPRSGTSIWADLWVSPKETAKDGLSSQWIDFCL
QPNIARQIALLTKTNSPVATNIGTADIQESLGGLFLNSQEVFAKGEFLLPLSPEATKQYEALFAKIKG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=221, Percent_Identity=24.8868778280543, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787078, Length=264, Percent_Identity=26.5151515151515, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 44216; Mature: 44216

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIPKSMDRRTFLVSLGGLTLSQLLVGCTGNNQTQLNVQLLKGSIPGQVVNKFRQSLKQQ
CCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
AQLKFAPVEQLEELYKRLVNWQHNPKAHDEQEWTRFIPFRQSQTFPVADLVTLGDYWLTT
HCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
AIERKLIQAIETEQLKQWSGLDSRWRELVTRNEQGVPDPQGKVWAAPYRWGSTVIVYDRE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCEEEEECHH
KFKKLGWIPQDWSDLWRSELRSRISVLDQPREIIGLVLKKLGKSYNTENLATVPDLEKEL
HHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
QTFNQQVKIYSSNTYLEPLILGDTWLAVGWSSDLIPVLARYPQLSVVIPRSGTSIWADLW
HHHHHEEEEEECCCEECEEEECCEEEEECCCCCHHHHHHCCCCEEEEEECCCCEEEEEEE
VSPKETAKDGLSSQWIDFCLQPNIARQIALLTKTNSPVATNIGTADIQESLGGLFLNSQE
CCCHHHHHCCCHHHHHHHHCCCCHHHHEEEEEECCCCCCCCCCHHHHHHHHCCEEECCHH
VFAKGEFLLPLSPEATKQYEALFAKIKG
HHCCCCEEEECCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYIPKSMDRRTFLVSLGGLTLSQLLVGCTGNNQTQLNVQLLKGSIPGQVVNKFRQSLKQQ
CCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHH
AQLKFAPVEQLEELYKRLVNWQHNPKAHDEQEWTRFIPFRQSQTFPVADLVTLGDYWLTT
HCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
AIERKLIQAIETEQLKQWSGLDSRWRELVTRNEQGVPDPQGKVWAAPYRWGSTVIVYDRE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCEEEEECHH
KFKKLGWIPQDWSDLWRSELRSRISVLDQPREIIGLVLKKLGKSYNTENLATVPDLEKEL
HHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
QTFNQQVKIYSSNTYLEPLILGDTWLAVGWSSDLIPVLARYPQLSVVIPRSGTSIWADLW
HHHHHEEEEEECCCEECEEEECCEEEEECCCCCHHHHHHCCCCEEEEEECCCCEEEEEEE
VSPKETAKDGLSSQWIDFCLQPNIARQIALLTKTNSPVATNIGTADIQESLGGLFLNSQE
CCCHHHHHCCCHHHHHHHHCCCCHHHHEEEEEECCCCCCCCCCHHHHHHHHCCEEECCHH
VFAKGEFLLPLSPEATKQYEALFAKIKG
HHCCCCEEEECCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]