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The map label for this gene is ppnK

Identifier: 17227723

GI number: 17227723

Start: 242623

End: 243543

Strand: Direct

Name: ppnK

Synonym: alr0227

Alternate gene names: 17227723

Gene position: 242623-243543 (Clockwise)

Preceding gene: 17227722

Following gene: 17227724

Centisome position: 3.78

GC content: 45.82

Gene sequence:

>921_bases
GTGCAACTCAAGCAGGTAATCATTGCTTATAAGGCGCGAGATTCCCAAAGCAAACGTTGGGCAGAACTCTGCGCCAAGCA
GCTAGAAAATCGCAATTGTCAGGTCTTAATGGGACCTAGCGGGCCAAAAGATAACCCTTATCCGGTGTTTTTGGCTTCAG
CTAGTCAACCAATCGACCTGGCAATTGTCTTAGGTGGTGATGGCACAGTCTTAACTAGTGCCAGACATTTAGCCCCAGCT
GGTATCCCCATTCTCGGCGTAAATGTGGGGGGTCACCTGGGCTTTTTAACCGAGTCAGTTGATGAATTTCAAGACACAGA
GAAAGTTTGGGATCGTCTATTTGAAGACCGCTACGCTATTCAACGGCGGATGATGTTGCAAGCGGCAGTTTATGAAGGAC
ACCGAACGAATCTGGAACCGGTAACAGAACGATATCTTGGGTTAAATGAATTTTGCGTCAAACCAGCCTCAGCCGATCGC
ATGATTACCTCTATTCTAGAAATGGAAATTGATGGTGAGGTAGTAGATCAATATGTAGGCGATGGATTGATTATTTCCAC
ACCCACAGGTTCAACTGGTTATACAGTCTCTGCAAGTGGCCCAATTATGCACGATGGTATGGAGGCAATTACCATCACGC
CTATTTGCCCTATGAGTCTTTCTAGCCGTCCTCTCATACTTCCCGCAGGTTCTGTTGTCAGTATCTGGCCGTTAGGAGAC
TATGATTTGAGTACCAAATTGTGGATGGATGGGGTTTTAGCTACTTCAATTTGGCCTGCACATCGCGTTGATATCCGCAT
GGCTGATTGTCGGGCAAAATTTATTGTGTTACGGGAAAATAATTCCTACTATCAAACGTTGCGAGAGAAATTACTGTGGG
CTGGTACTAGGGTTCGCTACACTAGTACTCAACACAATTGA

Upstream 100 bases:

>100_bases
TGCTGAAGCGGCGGTTGGTCTAGCGATCGTGCTGGCCATTTATCGCAACCGCGATACCGTCGATATGGAGCAGTTTAATC
TCCTGAAATGGTAATTGCGT

Downstream 100 bases:

>100_bases
GTAATTTATTTTGTAATTTTTAATTCGTAATTCGTAATTAATCATGAGATTACGAGTTACGAATTTTACTGAATATGAGT
AGATGTTGAGTCAACAGTTA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase 2

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MQLKQVIIAYKARDSQSKRWAELCAKQLENRNCQVLMGPSGPKDNPYPVFLASASQPIDLAIVLGGDGTVLTSARHLAPA
GIPILGVNVGGHLGFLTESVDEFQDTEKVWDRLFEDRYAIQRRMMLQAAVYEGHRTNLEPVTERYLGLNEFCVKPASADR
MITSILEMEIDGEVVDQYVGDGLIISTPTGSTGYTVSASGPIMHDGMEAITITPICPMSLSSRPLILPAGSVVSIWPLGD
YDLSTKLWMDGVLATSIWPAHRVDIRMADCRAKFIVLRENNSYYQTLREKLLWAGTRVRYTSTQHN

Sequences:

>Translated_306_residues
MQLKQVIIAYKARDSQSKRWAELCAKQLENRNCQVLMGPSGPKDNPYPVFLASASQPIDLAIVLGGDGTVLTSARHLAPA
GIPILGVNVGGHLGFLTESVDEFQDTEKVWDRLFEDRYAIQRRMMLQAAVYEGHRTNLEPVTERYLGLNEFCVKPASADR
MITSILEMEIDGEVVDQYVGDGLIISTPTGSTGYTVSASGPIMHDGMEAITITPICPMSLSSRPLILPAGSVVSIWPLGD
YDLSTKLWMDGVLATSIWPAHRVDIRMADCRAKFIVLRENNSYYQTLREKLLWAGTRVRYTSTQHN
>Mature_306_residues
MQLKQVIIAYKARDSQSKRWAELCAKQLENRNCQVLMGPSGPKDNPYPVFLASASQPIDLAIVLGGDGTVLTSARHLAPA
GIPILGVNVGGHLGFLTESVDEFQDTEKVWDRLFEDRYAIQRRMMLQAAVYEGHRTNLEPVTERYLGLNEFCVKPASADR
MITSILEMEIDGEVVDQYVGDGLIISTPTGSTGYTVSASGPIMHDGMEAITITPICPMSLSSRPLILPAGSVVSIWPLGD
YDLSTKLWMDGVLATSIWPAHRVDIRMADCRAKFIVLRENNSYYQTLREKLLWAGTRVRYTSTQHN

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=269, Percent_Identity=28.996282527881, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1788968, Length=246, Percent_Identity=36.1788617886179, Blast_Score=121, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6320794, Length=187, Percent_Identity=35.8288770053476, Blast_Score=109, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6322509, Length=238, Percent_Identity=31.0924369747899, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6325068, Length=177, Percent_Identity=35.0282485875706, Blast_Score=96, Evalue=5e-21,
Organism=Drosophila melanogaster, GI28573832, Length=191, Percent_Identity=32.4607329842932, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28573830, Length=191, Percent_Identity=32.4607329842932, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI161077047, Length=191, Percent_Identity=32.4607329842932, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI28573828, Length=191, Percent_Identity=32.4607329842932, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI28573826, Length=191, Percent_Identity=32.4607329842932, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI281363323, Length=233, Percent_Identity=30.0429184549356, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24653422, Length=233, Percent_Identity=30.0429184549356, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI281363321, Length=233, Percent_Identity=30.0429184549356, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI20129957, Length=233, Percent_Identity=30.0429184549356, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24653424, Length=233, Percent_Identity=30.4721030042918, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK2_ANAVT (Q3M9K5)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_323226.1
- ProteinModelPortal:   Q3M9K5
- SMR:   Q3M9K5
- STRING:   Q3M9K5
- GeneID:   3682079
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_2718
- eggNOG:   COG0061
- HOGENOM:   HBG713904
- OMA:   KLHWAGS
- PhylomeDB:   Q3M9K5
- ProtClustDB:   PRK02645
- BioCyc:   AVAR240292:AVA_2718-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 33933; Mature: 33933

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLKQVIIAYKARDSQSKRWAELCAKQLENRNCQVLMGPSGPKDNPYPVFLASASQPIDL
CCHHHEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEECCCCCEEE
AIVLGGDGTVLTSARHLAPAGIPILGVNVGGHLGFLTESVDEFQDTEKVWDRLFEDRYAI
EEEECCCCEEEECHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRRMMLQAAVYEGHRTNLEPVTERYLGLNEFCVKPASADRMITSILEMEIDGEVVDQYVG
HHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHCC
DGLIISTPTGSTGYTVSASGPIMHDGMEAITITPICPMSLSSRPLILPAGSVVSIWPLGD
CCEEEECCCCCCCEEEECCCCEEECCCCEEEEECCCCCCCCCCEEEEECCCEEEEEECCC
YDLSTKLWMDGVLATSIWPAHRVDIRMADCRAKFIVLRENNSYYQTLREKLLWAGTRVRY
CCCCHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEEECCCHHHHHHHHHHHHCCCEEEE
TSTQHN
ECCCCC
>Mature Secondary Structure
MQLKQVIIAYKARDSQSKRWAELCAKQLENRNCQVLMGPSGPKDNPYPVFLASASQPIDL
CCHHHEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEECCCCCEEE
AIVLGGDGTVLTSARHLAPAGIPILGVNVGGHLGFLTESVDEFQDTEKVWDRLFEDRYAI
EEEECCCCEEEECHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRRMMLQAAVYEGHRTNLEPVTERYLGLNEFCVKPASADRMITSILEMEIDGEVVDQYVG
HHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHCC
DGLIISTPTGSTGYTVSASGPIMHDGMEAITITPICPMSLSSRPLILPAGSVVSIWPLGD
CCEEEECCCCCCCEEEECCCCEEECCCCEEEEECCCCCCCCCCEEEEECCCEEEEEECCC
YDLSTKLWMDGVLATSIWPAHRVDIRMADCRAKFIVLRENNSYYQTLREKLLWAGTRVRY
CCCCHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEEECCCHHHHHHHHHHHHCCCEEEE
TSTQHN
ECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA