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The map label for this gene is ndhI [H]

Identifier: 17227720

GI number: 17227720

Start: 240980

End: 241564

Strand: Direct

Name: ndhI [H]

Synonym: alr0224

Alternate gene names: 17227720

Gene position: 240980-241564 (Clockwise)

Preceding gene: 17227719

Following gene: 17227721

Centisome position: 3.76

GC content: 46.32

Gene sequence:

>585_bases
ATGCTAAAGTTCCTCAAGCAAGTTGGTGATTACGCCAAAGAAGCAGTACAAGCAGGTCGTTATATTGGTCAAGGGCTATC
TGTAACTTTTGACCATATGCGCCGCCGTCCTGTCACCGTACAGTACCCTTATGAGAAACTCATTCCTGGTGAACGCTTTC
GTGGTCGGATTCACTACGAATTTGATAAGTGTATCGCTTGTGAAGTCTGTGTAAGGGTTTGTCCTATCAATTTACCTGTA
GTTGATTGGGAATTCGACAAAGCTACCAAGAAGAAAAAGCTTAACCACTATAGTATCGATTTTGGGGTCTGTATTTTCTG
CGGTAACTGTGTTGAGTACTGCCCGACAAACTGTCTATCAATGACAGAAGAGTACGAATTGGCAACTTACGATCGCCACG
AACTCAACTATGATAGTGTGGCTTTAGGTCGTCTCCCCTACAAAGTTACCGACGACCCAATGGTGACACCACTCCGGGAA
CTAGTTTACCTACCCAAAGGTGTACTTGACCCCCACGACCTACCCGCCAATGCACCGCGCCCTGGCGCACGTCCAGAAGA
CTTGGTAGAAAAAACAGAAGCATGA

Upstream 100 bases:

>100_bases
TTCGGCGGGTAATCGGTAATCGGTAATCGGTAATGGAAAAATCATTACCAATTACCTGTTACCAATTACCAATTACCTCA
AAAGAGTAGAGAGACACAAA

Downstream 100 bases:

>100_bases
TAGTAGTCAATAGTCGGTAGTCAGTAGTCAGTGGCAAAAAATATGAAATAGCGACTGACAACTGACAACTGACAACCAAC
AACTGACAGAGGAAAAAAAA

Product: NADH dehydrogenase subunit I

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I subunit I; NDH-1 subunit I; NDH-I [H]

Number of amino acids: Translated: 194; Mature: 194

Protein sequence:

>194_residues
MLKFLKQVGDYAKEAVQAGRYIGQGLSVTFDHMRRRPVTVQYPYEKLIPGERFRGRIHYEFDKCIACEVCVRVCPINLPV
VDWEFDKATKKKKLNHYSIDFGVCIFCGNCVEYCPTNCLSMTEEYELATYDRHELNYDSVALGRLPYKVTDDPMVTPLRE
LVYLPKGVLDPHDLPANAPRPGARPEDLVEKTEA

Sequences:

>Translated_194_residues
MLKFLKQVGDYAKEAVQAGRYIGQGLSVTFDHMRRRPVTVQYPYEKLIPGERFRGRIHYEFDKCIACEVCVRVCPINLPV
VDWEFDKATKKKKLNHYSIDFGVCIFCGNCVEYCPTNCLSMTEEYELATYDRHELNYDSVALGRLPYKVTDDPMVTPLRE
LVYLPKGVLDPHDLPANAPRPGARPEDLVEKTEA
>Mature_194_residues
MLKFLKQVGDYAKEAVQAGRYIGQGLSVTFDHMRRRPVTVQYPYEKLIPGERFRGRIHYEFDKCIACEVCVRVCPINLPV
VDWEFDKATKKKKLNHYSIDFGVCIFCGNCVEYCPTNCLSMTEEYELATYDRHELNYDSVALGRLPYKVTDDPMVTPLRE
LVYLPKGVLDPHDLPANAPRPGARPEDLVEKTEA

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cellular thylakoid membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=143, Percent_Identity=31.4685314685315, Blast_Score=78, Evalue=4e-15,
Organism=Escherichia coli, GI1789075, Length=102, Percent_Identity=36.2745098039216, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788617, Length=118, Percent_Identity=32.2033898305085, Blast_Score=64, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17555194, Length=124, Percent_Identity=31.4516129032258, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17864306, Length=124, Percent_Identity=33.0645161290323, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR004497
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 22283; Mature: 22283

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
4.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFLKQVGDYAKEAVQAGRYIGQGLSVTFDHMRRRPVTVQYPYEKLIPGERFRGRIHYE
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCEEECCHHHCCCCHHHCCEEEEC
FDKCIACEVCVRVCPINLPVVDWEFDKATKKKKLNHYSIDFGVCIFCGNCVEYCPTNCLS
HHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHCCCCCCCHHHEEHCCCHHHHCCCHHHH
MTEEYELATYDRHELNYDSVALGRLPYKVTDDPMVTPLRELVYLPKGVLDPHDLPANAPR
HHHHHHHHCCCCCCCCCCCEEECCCCCEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
PGARPEDLVEKTEA
CCCCHHHHHHHCCC
>Mature Secondary Structure
MLKFLKQVGDYAKEAVQAGRYIGQGLSVTFDHMRRRPVTVQYPYEKLIPGERFRGRIHYE
CHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCEEECCHHHCCCCHHHCCEEEEC
FDKCIACEVCVRVCPINLPVVDWEFDKATKKKKLNHYSIDFGVCIFCGNCVEYCPTNCLS
HHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHCCCCCCCHHHEEHCCCHHHHCCCHHHH
MTEEYELATYDRHELNYDSVALGRLPYKVTDDPMVTPLRELVYLPKGVLDPHDLPANAPR
HHHHHHHHCCCCCCCCCCCEEECCCCCEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
PGARPEDLVEKTEA
CCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA