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The map label for this gene is gltA [H]

Identifier: 17227718

GI number: 17227718

Start: 238171

End: 239307

Strand: Direct

Name: gltA [H]

Synonym: alr0222

Alternate gene names: 17227718

Gene position: 238171-239307 (Clockwise)

Preceding gene: 17227717

Following gene: 17227719

Centisome position: 3.71

GC content: 46.97

Gene sequence:

>1137_bases
ATGATGGTGTGCGAATACAAGCCTGGTTTAGAAGGCATTCCCGCCGCCCAATCGAGTATCAGTTATGTAGATGGGCAAAA
GGGAATACTAGAATATCGTGGCATCCGGATTGAGGATTTAGCCCAGCAAAGTACTTTTCTGGAGACTGCTTATCTTTTAA
TCTGGGGTGAGTTGCCAACAAAAGAAGAATTGCAAGTATTTGAGGAGGAAGTCCGTCTTCATCGGCGGATTAAATACCGG
ATTCGGGATATGATGAAGTGCTTTCCCGAATCTGGTCATCCAATGGATGCACTCCAAGCCTCTGCGGCGGCTTTAGGCTT
GTTTTACTCCCGTCGAGATTTGCACAATCCTGCCTATATTCGGGATGCTGTAGTGCGGCTAATAGCTACTATTCCGACGA
TGGTAGCTGCATTCCAGTTGATGCGGAAAGGTAATGACCCCGTTAAGCCCCGTGATGATTTAGATTATTCCGCCAATTTT
CTCTACATGCTCAACGAGAAAGAACCGGATGCTTTGGCGGCAAAAATCTTTGATATCTGCTTGATTCTCCATGTCGAGCA
TACGATGAATGCTTCCACCTTTAGTGCTAGGGTAACAGCTTCCACCTTGACTGACCCGTATGCGGTGGTTGCTAGCGCTG
TGGGGACTTTAGGAGGGCCTTTACACGGTGGAGCCAATGAAGAAGTAATCCAGATGTTGGAAGAGATTGGTTCCGTGGAG
AATGTGCGTTCTTATGTCGAGGAGAGGTTGCAACGTAAAGACAAGCTCATGGGCTTTGGACATCGTGTCTACAAAGTTAA
AGACCCACGGGCGACAATTTTGCAAGGCCTCGCAGAACAGTTGTTTGCCAAGTTCGGCGCAGATAAGTATTACGACATCG
CCCAAGAAATGGAACGGGTAGTCGAAGAGAAACTTGGTCATAAAGGGATTTATCCCAATGTTGACTTCTACTCTGGTTTA
GTGTATCGGAAGATGGGTATTCCTACAGACTTGTTTACACCAATCTTTGCGATCGCTCGTGTTGCTGGTTGGTTAGCCCA
CTGGAAAGAACAACTCGAAGAGAACCGCATTTTCCGTCCTACCCAGGTTTACAACGGCAAACACAGTGTTACCTACACCC
CCATTGACCAACGTTAA

Upstream 100 bases:

>100_bases
GTTCGGCCAAGAATTGTTGTGAGCTAGGCTAGTGTTATGGCGGCAATAATTTCTGGTAAGTTAGCTTGATCAGATATTTC
ACTAAGCAAATGGTGTTGCC

Downstream 100 bases:

>100_bases
AAGCTGGGGAATAAGAGGTATCAAGTAGGGAAAGAGTTGTGATTTTTCTTAACTTGCCCCCAGTGTCGGGAATCAGTCAA
TCAATAGCCATCTGATAACC

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MMVCEYKPGLEGIPAAQSSISYVDGQKGILEYRGIRIEDLAQQSTFLETAYLLIWGELPTKEELQVFEEEVRLHRRIKYR
IRDMMKCFPESGHPMDALQASAAALGLFYSRRDLHNPAYIRDAVVRLIATIPTMVAAFQLMRKGNDPVKPRDDLDYSANF
LYMLNEKEPDALAAKIFDICLILHVEHTMNASTFSARVTASTLTDPYAVVASAVGTLGGPLHGGANEEVIQMLEEIGSVE
NVRSYVEERLQRKDKLMGFGHRVYKVKDPRATILQGLAEQLFAKFGADKYYDIAQEMERVVEEKLGHKGIYPNVDFYSGL
VYRKMGIPTDLFTPIFAIARVAGWLAHWKEQLEENRIFRPTQVYNGKHSVTYTPIDQR

Sequences:

>Translated_378_residues
MMVCEYKPGLEGIPAAQSSISYVDGQKGILEYRGIRIEDLAQQSTFLETAYLLIWGELPTKEELQVFEEEVRLHRRIKYR
IRDMMKCFPESGHPMDALQASAAALGLFYSRRDLHNPAYIRDAVVRLIATIPTMVAAFQLMRKGNDPVKPRDDLDYSANF
LYMLNEKEPDALAAKIFDICLILHVEHTMNASTFSARVTASTLTDPYAVVASAVGTLGGPLHGGANEEVIQMLEEIGSVE
NVRSYVEERLQRKDKLMGFGHRVYKVKDPRATILQGLAEQLFAKFGADKYYDIAQEMERVVEEKLGHKGIYPNVDFYSGL
VYRKMGIPTDLFTPIFAIARVAGWLAHWKEQLEENRIFRPTQVYNGKHSVTYTPIDQR
>Mature_378_residues
MMVCEYKPGLEGIPAAQSSISYVDGQKGILEYRGIRIEDLAQQSTFLETAYLLIWGELPTKEELQVFEEEVRLHRRIKYR
IRDMMKCFPESGHPMDALQASAAALGLFYSRRDLHNPAYIRDAVVRLIATIPTMVAAFQLMRKGNDPVKPRDDLDYSANF
LYMLNEKEPDALAAKIFDICLILHVEHTMNASTFSARVTASTLTDPYAVVASAVGTLGGPLHGGANEEVIQMLEEIGSVE
NVRSYVEERLQRKDKLMGFGHRVYKVKDPRATILQGLAEQLFAKFGADKYYDIAQEMERVVEEKLGHKGIYPNVDFYSGL
VYRKMGIPTDLFTPIFAIARVAGWLAHWKEQLEENRIFRPTQVYNGKHSVTYTPIDQR

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=381, Percent_Identity=27.5590551181102, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI1786939, Length=376, Percent_Identity=38.8297872340425, Blast_Score=280, Evalue=7e-77,
Organism=Escherichia coli, GI1786527, Length=370, Percent_Identity=34.8648648648649, Blast_Score=254, Evalue=7e-69,
Organism=Caenorhabditis elegans, GI17555174, Length=378, Percent_Identity=26.1904761904762, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6319850, Length=371, Percent_Identity=25.6064690026954, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6325257, Length=386, Percent_Identity=25.1295336787565, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6324328, Length=367, Percent_Identity=24.5231607629428, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24640124, Length=371, Percent_Identity=27.2237196765499, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24640126, Length=371, Percent_Identity=27.2237196765499, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI21356863, Length=380, Percent_Identity=27.3684210526316, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 42935; Mature: 42935

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMVCEYKPGLEGIPAAQSSISYVDGQKGILEYRGIRIEDLAQQSTFLETAYLLIWGELPT
CEEECCCCCCCCCCCCHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCC
KEELQVFEEEVRLHRRIKYRIRDMMKCFPESGHPMDALQASAAALGLFYSRRDLHNPAYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
RDAVVRLIATIPTMVAAFQLMRKGNDPVKPRDDLDYSANFLYMLNEKEPDALAAKIFDIC
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
LILHVEHTMNASTFSARVTASTLTDPYAVVASAVGTLGGPLHGGANEEVIQMLEEIGSVE
HHHHHHHHCCCCHHHHEEEHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHH
NVRSYVEERLQRKDKLMGFGHRVYKVKDPRATILQGLAEQLFAKFGADKYYDIAQEMERV
HHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VEEKLGHKGIYPNVDFYSGLVYRKMGIPTDLFTPIFAIARVAGWLAHWKEQLEENRIFRP
HHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TQVYNGKHSVTYTPIDQR
HHHCCCCCCEEECCCCCC
>Mature Secondary Structure
MMVCEYKPGLEGIPAAQSSISYVDGQKGILEYRGIRIEDLAQQSTFLETAYLLIWGELPT
CEEECCCCCCCCCCCCHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCC
KEELQVFEEEVRLHRRIKYRIRDMMKCFPESGHPMDALQASAAALGLFYSRRDLHNPAYI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHH
RDAVVRLIATIPTMVAAFQLMRKGNDPVKPRDDLDYSANFLYMLNEKEPDALAAKIFDIC
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHH
LILHVEHTMNASTFSARVTASTLTDPYAVVASAVGTLGGPLHGGANEEVIQMLEEIGSVE
HHHHHHHHCCCCHHHHEEEHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHH
NVRSYVEERLQRKDKLMGFGHRVYKVKDPRATILQGLAEQLFAKFGADKYYDIAQEMERV
HHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VEEKLGHKGIYPNVDFYSGLVYRKMGIPTDLFTPIFAIARVAGWLAHWKEQLEENRIFRP
HHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TQVYNGKHSVTYTPIDQR
HHHCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]