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The map label for this gene is trmH [C]

Identifier: 17227671

GI number: 17227671

Start: 185196

End: 186017

Strand: Direct

Name: trmH [C]

Synonym: alr0175

Alternate gene names: 17227671

Gene position: 185196-186017 (Clockwise)

Preceding gene: 17227665

Following gene: 17227675

Centisome position: 2.89

GC content: 46.72

Gene sequence:

>822_bases
GTGCTGACGTTGTTAAATATTCAGACCTCAGCATTAGCGATGTTAACAAGTTTACAAAATTCATTAGTTAAACAAATCCG
CAAGCTGCACTCTACCAAAGAACGGCATAAACAAGGGCTGTTCTTGTTGGAAGGGACGCACTTGTTAGAAGAAGCTTGTG
CAATGGATTATCCCCTAGAGGTGGTGTGTTGTACTCCAGAATGGGAGGCAAACCATACAGCTTTGTGGGATGATGCTTGT
AGTCGATGCGATCGCTATGAGATAGTCAGTGAAGAAGTATTACAGGCGATCGCTACTACTGTACAACCAGATGGGGTAGT
GGCAACGGCGAAGCGCAGCGAACAGCAAAGCCAAGTACCATTTACCGGCGTAGTCCTAGCTTTGGAAACCATCCAAGACC
CTGGTAATTTAGGGACAATCATTCGCACGGCGGCGGCGGCTGGTGCATCGGGGTTATTGCTGAGTCAAGATAGTGTAGAT
TTAGATAACCCCAAGGTTCTAAGGGCTTCGGCTGGACAGTGGTTTCGTCTAGCCACCGCAGTTAGTGAAGATTTAAAAGC
AACCGTGCAGCTTTGCCAGCAAGCAGGAATGCAGGTAGTAGCCACCTTACCCACCGCTAAGTTAACTTATTGGGAAATAG
ACTGGCGCAAACCCAGCTTAATTTTATTAGGCAATGAAGGCGCTGGCTTGTCGCCTGATTTAGCGGCGATGGCAGACCAG
GAAGTAAAAATTCCCCTAAGTCCTGGTGTAGAATCTTTGAATGTAGCAATTACATCCGCCTTAATGTTGTACGAAGTACA
GCGACAGTTAAACTTGCAATAG

Upstream 100 bases:

>100_bases
CAAGCGGAACTGGCGGAATTGGCAGACGCGCTAGATTCAGGTTCTAGTGCCGCAAGGCTTCCGGGTTCAAGTCCCGGGTT
CCGCATCAATTCAGTGCTGA

Downstream 100 bases:

>100_bases
GTAATACATTAATGTTGCTGTGTTGGGTGGCGACTATTTGGACTTTGTGATTTCTTCTAAATAATTTTCAATATCAGCAA
CCGCATCTTCCAAGGCTGCC

Product: rRNA methylase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MLTLLNIQTSALAMLTSLQNSLVKQIRKLHSTKERHKQGLFLLEGTHLLEEACAMDYPLEVVCCTPEWEANHTALWDDAC
SRCDRYEIVSEEVLQAIATTVQPDGVVATAKRSEQQSQVPFTGVVLALETIQDPGNLGTIIRTAAAAGASGLLLSQDSVD
LDNPKVLRASAGQWFRLATAVSEDLKATVQLCQQAGMQVVATLPTAKLTYWEIDWRKPSLILLGNEGAGLSPDLAAMADQ
EVKIPLSPGVESLNVAITSALMLYEVQRQLNLQ

Sequences:

>Translated_273_residues
MLTLLNIQTSALAMLTSLQNSLVKQIRKLHSTKERHKQGLFLLEGTHLLEEACAMDYPLEVVCCTPEWEANHTALWDDAC
SRCDRYEIVSEEVLQAIATTVQPDGVVATAKRSEQQSQVPFTGVVLALETIQDPGNLGTIIRTAAAAGASGLLLSQDSVD
LDNPKVLRASAGQWFRLATAVSEDLKATVQLCQQAGMQVVATLPTAKLTYWEIDWRKPSLILLGNEGAGLSPDLAAMADQ
EVKIPLSPGVESLNVAITSALMLYEVQRQLNLQ
>Mature_273_residues
MLTLLNIQTSALAMLTSLQNSLVKQIRKLHSTKERHKQGLFLLEGTHLLEEACAMDYPLEVVCCTPEWEANHTALWDDAC
SRCDRYEIVSEEVLQAIATTVQPDGVVATAKRSEQQSQVPFTGVVLALETIQDPGNLGTIIRTAAAAGASGLLLSQDSVD
LDNPKVLRASAGQWFRLATAVSEDLKATVQLCQQAGMQVVATLPTAKLTYWEIDWRKPSLILLGNEGAGLSPDLAAMADQ
EVKIPLSPGVESLNVAITSALMLYEVQRQLNLQ

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=156, Percent_Identity=30.7692307692308, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1790083, Length=147, Percent_Identity=33.3333333333333, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790623, Length=150, Percent_Identity=26.6666666666667, Blast_Score=63, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24666840, Length=300, Percent_Identity=25, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 29650; Mature: 29650

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLLNIQTSALAMLTSLQNSLVKQIRKLHSTKERHKQGLFLLEGTHLLEEACAMDYPLE
CEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHCCCCCEE
VVCCTPEWEANHTALWDDACSRCDRYEIVSEEVLQAIATTVQPDGVVATAKRSEQQSQVP
EEEECCCCCCCCCEEHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEECHHCHHHHCCC
FTGVVLALETIQDPGNLGTIIRTAAAAGASGLLLSQDSVDLDNPKVLRASAGQWFRLATA
HHHHHEEHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEECCCCHHHHHHH
VSEDLKATVQLCQQAGMQVVATLPTAKLTYWEIDWRKPSLILLGNEGAGLSPDLAAMADQ
HHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEECCCCCEEEEECCCCCCCCCHHHHCCC
EVKIPLSPGVESLNVAITSALMLYEVQRQLNLQ
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTLLNIQTSALAMLTSLQNSLVKQIRKLHSTKERHKQGLFLLEGTHLLEEACAMDYPLE
CEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECHHHHHHHHCCCCCEE
VVCCTPEWEANHTALWDDACSRCDRYEIVSEEVLQAIATTVQPDGVVATAKRSEQQSQVP
EEEECCCCCCCCCEEHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEECHHCHHHHCCC
FTGVVLALETIQDPGNLGTIIRTAAAAGASGLLLSQDSVDLDNPKVLRASAGQWFRLATA
HHHHHEEHHHHCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEECCCCHHHHHHH
VSEDLKATVQLCQQAGMQVVATLPTAKLTYWEIDWRKPSLILLGNEGAGLSPDLAAMADQ
HHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEECCCCCEEEEECCCCCCCCCHHHHCCC
EVKIPLSPGVESLNVAITSALMLYEVQRQLNLQ
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]