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The map label for this gene is rnhA [H]

Identifier: 17227638

GI number: 17227638

Start: 147532

End: 148440

Strand: Direct

Name: rnhA [H]

Synonym: alr0142

Alternate gene names: 17227638

Gene position: 147532-148440 (Clockwise)

Preceding gene: 17227637

Following gene: 17227641

Centisome position: 2.3

GC content: 44.99

Gene sequence:

>909_bases
ATGTCTACCCAACCGATAATCGAAAGTATATACACTGATGGTGCTTGCACTGGCAATCCTGGCCCTGGCGGCTGGGGTGT
AGTTGTCTATTTTAGTGATGGTTCAGTTCACGAAATGGGCGATGCTGCCAAGCATACCACCAATAATAAGATGGAAATGC
AGGCGGCGATCGCCGCTCTCAAATTTCTCCACGACTCCGGACAAGCTGAACCCATTACCCTCTATACCGATAGTGAGTAT
CTAATTAACTGCGTCACCAAGTGGGTAAAAGGTTGGAAAAAAAAGGGTTGGAAAAAATCTGATGGGAATCCAGTTCAGAA
TCAAGACCTTCTAGAAACTTTAGATGAATTAAATAGCCGTAAGGTAAATTGGCATCATGTTCGAGGTCATTCTGGCAACA
TCGGTAACGAGCGTTGTGATGTGATTGCTCGCTGTTTTGCAACTGGTAGAATGCCCTCTCTACAACAGTTATCTCCCCGT
CATGCTCATAAATCTTTACCTGACATCAGTAAAATAAATGTAGTTAAAGTACCTGATCATGTTACAACCTCTACAATAGT
TCACAAAACGACACAAGAAATCAGTACTTCTGCGTCAAATATCAACACTATGGAACCATCTACCGCACACGTTGCGGCTA
CAATCGAGGAAAACCCGCCGGAAAAGCGGGTGGAACAACTCCGCAACTTAGTAGAAACTCTGCATATTGCAGACGAAATT
GCGGCGAAAGGTTATTTAATCACTAGTTCTGAGTTGGCAGACTTAATGGATGTCCACGCCAGTGCTGTTACTAGTAGGGG
AGATCAGTGGCGCTGGCGCAACTGGATTGTTTCACGGGTACGCCGTGAGGGTAATCAAATTCTCTGGGAATTAGAACGAG
GCGATCGCTTGGGGGGTGAAGATGAATAA

Upstream 100 bases:

>100_bases
CACGTATTCGTTATTAAGGAAATTAGTAGTAATAGCCTAGATATTGCTAAATAAAATGCGTGGCTAATAGCTCTTCGCTC
TGTGCTTTGATGTTTTTACT

Downstream 100 bases:

>100_bases
ATAATTGGGGTAGGGTCTTGGGAAAATAGAGGCAGGAATTATTGCTCTCTCCTCAGCACTCACTATTCCTTGCTATAAGT
TCTACCTCTCCATTCCCTGG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MSTQPIIESIYTDGACTGNPGPGGWGVVVYFSDGSVHEMGDAAKHTTNNKMEMQAAIAALKFLHDSGQAEPITLYTDSEY
LINCVTKWVKGWKKKGWKKSDGNPVQNQDLLETLDELNSRKVNWHHVRGHSGNIGNERCDVIARCFATGRMPSLQQLSPR
HAHKSLPDISKINVVKVPDHVTTSTIVHKTTQEISTSASNINTMEPSTAHVAATIEENPPEKRVEQLRNLVETLHIADEI
AAKGYLITSSELADLMDVHASAVTSRGDQWRWRNWIVSRVRREGNQILWELERGDRLGGEDE

Sequences:

>Translated_302_residues
MSTQPIIESIYTDGACTGNPGPGGWGVVVYFSDGSVHEMGDAAKHTTNNKMEMQAAIAALKFLHDSGQAEPITLYTDSEY
LINCVTKWVKGWKKKGWKKSDGNPVQNQDLLETLDELNSRKVNWHHVRGHSGNIGNERCDVIARCFATGRMPSLQQLSPR
HAHKSLPDISKINVVKVPDHVTTSTIVHKTTQEISTSASNINTMEPSTAHVAATIEENPPEKRVEQLRNLVETLHIADEI
AAKGYLITSSELADLMDVHASAVTSRGDQWRWRNWIVSRVRREGNQILWELERGDRLGGEDE
>Mature_301_residues
STQPIIESIYTDGACTGNPGPGGWGVVVYFSDGSVHEMGDAAKHTTNNKMEMQAAIAALKFLHDSGQAEPITLYTDSEYL
INCVTKWVKGWKKKGWKKSDGNPVQNQDLLETLDELNSRKVNWHHVRGHSGNIGNERCDVIARCFATGRMPSLQQLSPRH
AHKSLPDISKINVVKVPDHVTTSTIVHKTTQEISTSASNINTMEPSTAHVAATIEENPPEKRVEQLRNLVETLHIADEIA
AKGYLITSSELADLMDVHASAVTSRGDQWRWRNWIVSRVRREGNQILWELERGDRLGGEDE

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=139, Percent_Identity=39.568345323741, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1786408, Length=147, Percent_Identity=42.8571428571429, Blast_Score=124, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI115532542, Length=140, Percent_Identity=40.7142857142857, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17505482, Length=132, Percent_Identity=36.3636363636364, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17137476, Length=143, Percent_Identity=43.3566433566434, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45552509, Length=143, Percent_Identity=43.3566433566434, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 33624; Mature: 33493

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQPIIESIYTDGACTGNPGPGGWGVVVYFSDGSVHEMGDAAKHTTNNKMEMQAAIAAL
CCCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHCCHHHHCCCCHHHHHHHHHHH
KFLHDSGQAEPITLYTDSEYLINCVTKWVKGWKKKGWKKSDGNPVQNQDLLETLDELNSR
HHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
KVNWHHVRGHSGNIGNERCDVIARCFATGRMPSLQQLSPRHAHKSLPDISKINVVKVPDH
CCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCHHHHHHCCCCCCCEEEEECCCC
VTTSTIVHKTTQEISTSASNINTMEPSTAHVAATIEENPPEKRVEQLRNLVETLHIADEI
CHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
AAKGYLITSSELADLMDVHASAVTSRGDQWRWRNWIVSRVRREGNQILWELERGDRLGGE
HHCCEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
DE
CC
>Mature Secondary Structure 
STQPIIESIYTDGACTGNPGPGGWGVVVYFSDGSVHEMGDAAKHTTNNKMEMQAAIAAL
CCCHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHCCHHHHCCCCHHHHHHHHHHH
KFLHDSGQAEPITLYTDSEYLINCVTKWVKGWKKKGWKKSDGNPVQNQDLLETLDELNSR
HHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCC
KVNWHHVRGHSGNIGNERCDVIARCFATGRMPSLQQLSPRHAHKSLPDISKINVVKVPDH
CCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHCCHHHHHHCCCCCCCEEEEECCCC
VTTSTIVHKTTQEISTSASNINTMEPSTAHVAATIEENPPEKRVEQLRNLVETLHIADEI
CHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
AAKGYLITSSELADLMDVHASAVTSRGDQWRWRNWIVSRVRREGNQILWELERGDRLGGE
HHCCEEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
DE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA