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The map label for this gene is barA [H]

Identifier: 17227613

GI number: 17227613

Start: 119269

End: 120186

Strand: Direct

Name: barA [H]

Synonym: alr0117

Alternate gene names: 17227613

Gene position: 119269-120186 (Clockwise)

Preceding gene: 17227612

Following gene: 17227620

Centisome position: 1.86

GC content: 41.61

Gene sequence:

>918_bases
ATGAATTGGAGCAACTGGATATATCTAGGAGCCGGATTATTATTGGGGCTTGGTGTTCGTGGTTTATTTGCGCGGTCTAC
AAATGCCACTGCTAGCCAGTCCACAGTCACACTAGGGGAGCAAGAGTACACATCACAACTCCAGCAACAGCTCCAAAACA
CACAGCTAGCCTATGAAATGGCCCAAGAGATGAGCCAGTTTAAAGGGGGTTTCTTAGCACGGACTACCCATGAATTGCGA
TCGCCTCTCAATGGTCTCATTGGTCTACATCAGTTAATTTTGTCTGATCTTTGTGAAGATCCAGCAGAAGAGCGGGAATT
TATTACCCAAGCTCATGAGAGAGCTTTAAAGTTGCTGCATCTCATGGACGAAATCCTCAATGTAGCCAAGACAGAACACG
GTAACAATAAATTAGATATTCAGCCAAAATCTTTAGCCGAAATTTTACAAGAAGTATATAAATTAACTTATATGCTGGCG
GTGGATCGTAATTTAAAGTTGCAGATTTCGCCTCCCGACCCAGAGATTTATGTTTTGGCAGATGCAAGATGGCTAAGACA
GGTATTGTTAAACTTAGTAGACACTGCCATTAGTCAAATGCAGGAAGGAAGTATCTTTATTTCTGCGAAGCTTACACCTG
AAAGCGAATATGTTTCTATTTGGTTAGATTTACCTACTCATACTGTAGTTAAAAGTGAATCGATTGATTTACTCCGAATG
ACTGATGAATCTTTGCCGACCGATAAACGGAAAGCTGGTCTTTCTCCCAGTATGAAGCTATTACTTAATCAAAAACTGCT
AACAGTGATGGGAGGAAAGTTAGAAATCCTACCCTCTCCTGAATCCCATGCAGCAGAGCAATTAACTAGACTCGAATTGT
CTATCCCCCTAACGATTCCTGAAGCTGAACTTCTGTAG

Upstream 100 bases:

>100_bases
TGAAGAACTTGTTGGATCAAGCAGAACTGATGAACTAAAAAATAAGGAAACTGATTTTTATACTTTGTGGTCAATTTAAC
AAGTTAATAACTGTCTAAAT

Downstream 100 bases:

>100_bases
TAGTTAAAGAGCAAATTCGGGGTTGATTGTGTAGCACCATAGTGGTGCTGTTATTAGCTTGGAAGCCGATTTTTTCGTAA
AACTCCTGTCGATTGGTAGT

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNWSNWIYLGAGLLLGLGVRGLFARSTNATASQSTVTLGEQEYTSQLQQQLQNTQLAYEMAQEMSQFKGGFLARTTHELR
SPLNGLIGLHQLILSDLCEDPAEEREFITQAHERALKLLHLMDEILNVAKTEHGNNKLDIQPKSLAEILQEVYKLTYMLA
VDRNLKLQISPPDPEIYVLADARWLRQVLLNLVDTAISQMQEGSIFISAKLTPESEYVSIWLDLPTHTVVKSESIDLLRM
TDESLPTDKRKAGLSPSMKLLLNQKLLTVMGGKLEILPSPESHAAEQLTRLELSIPLTIPEAELL

Sequences:

>Translated_305_residues
MNWSNWIYLGAGLLLGLGVRGLFARSTNATASQSTVTLGEQEYTSQLQQQLQNTQLAYEMAQEMSQFKGGFLARTTHELR
SPLNGLIGLHQLILSDLCEDPAEEREFITQAHERALKLLHLMDEILNVAKTEHGNNKLDIQPKSLAEILQEVYKLTYMLA
VDRNLKLQISPPDPEIYVLADARWLRQVLLNLVDTAISQMQEGSIFISAKLTPESEYVSIWLDLPTHTVVKSESIDLLRM
TDESLPTDKRKAGLSPSMKLLLNQKLLTVMGGKLEILPSPESHAAEQLTRLELSIPLTIPEAELL
>Mature_305_residues
MNWSNWIYLGAGLLLGLGVRGLFARSTNATASQSTVTLGEQEYTSQLQQQLQNTQLAYEMAQEMSQFKGGFLARTTHELR
SPLNGLIGLHQLILSDLCEDPAEEREFITQAHERALKLLHLMDEILNVAKTEHGNNKLDIQPKSLAEILQEVYKLTYMLA
VDRNLKLQISPPDPEIYVLADARWLRQVLLNLVDTAISQMQEGSIFISAKLTPESEYVSIWLDLPTHTVVKSESIDLLRM
TDESLPTDKRKAGLSPSMKLLLNQKLLTVMGGKLEILPSPESHAAEQLTRLELSIPLTIPEAELL

Specific function: Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. Phosphorylates UvrY, probably via a four-step phosphorelay [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=244, Percent_Identity=29.9180327868852, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI48994928, Length=213, Percent_Identity=24.8826291079812, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081816, Length=243, Percent_Identity=24.6913580246914, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI145693157, Length=227, Percent_Identity=26.431718061674, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1788713, Length=145, Percent_Identity=31.7241379310345, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786912, Length=231, Percent_Identity=22.5108225108225, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR019247
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF09984 DUF2222; PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 34180; Mature: 34180

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNWSNWIYLGAGLLLGLGVRGLFARSTNATASQSTVTLGEQEYTSQLQQQLQNTQLAYEM
CCCCCEEEHHHHHHHHHCHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHH
AQEMSQFKGGFLARTTHELRSPLNGLIGLHQLILSDLCEDPAEEREFITQAHERALKLLH
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LMDEILNVAKTEHGNNKLDIQPKSLAEILQEVYKLTYMLAVDRNLKLQISPPDPEIYVLA
HHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCEEEEE
DARWLRQVLLNLVDTAISQMQEGSIFISAKLTPESEYVSIWLDLPTHTVVKSESIDLLRM
CHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCEEECCCCCCEEEE
TDESLPTDKRKAGLSPSMKLLLNQKLLTVMGGKLEILPSPESHAAEQLTRLELSIPLTIP
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHEEEEEEECC
EAELL
HHHCC
>Mature Secondary Structure
MNWSNWIYLGAGLLLGLGVRGLFARSTNATASQSTVTLGEQEYTSQLQQQLQNTQLAYEM
CCCCCEEEHHHHHHHHHCHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHH
AQEMSQFKGGFLARTTHELRSPLNGLIGLHQLILSDLCEDPAEEREFITQAHERALKLLH
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LMDEILNVAKTEHGNNKLDIQPKSLAEILQEVYKLTYMLAVDRNLKLQISPPDPEIYVLA
HHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCCCEEEEE
DARWLRQVLLNLVDTAISQMQEGSIFISAKLTPESEYVSIWLDLPTHTVVKSESIDLLRM
CHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEECCCCEEECCCCCCEEEE
TDESLPTDKRKAGLSPSMKLLLNQKLLTVMGGKLEILPSPESHAAEQLTRLELSIPLTIP
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHEEEEEEECC
EAELL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]