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The map label for this gene is znuA2 [H]

Identifier: 172087767

GI number: 172087767

Start: 2653991

End: 2654956

Strand: Reverse

Name: znuA2 [H]

Synonym: VF_2366

Alternate gene names: 172087767

Gene position: 2654956-2653991 (Counterclockwise)

Preceding gene: 59712979

Following gene: 59712970

Centisome position: 91.63

GC content: 40.06

Gene sequence:

>966_bases
ATGTTTAAAAAAGCCATATCAGTGTTTGTTATGAGCTTAGCGGTGGTTTCACAGAGTTACGCTCAAGCCCCAAAGGTTGT
CGTGGATATCGCTCCATTACACTCTTTAGTCACTCAAGTTATGGATGGCGTGGGTGAGCCGGATTTATTGATTCGTCCTG
AAATGTCACCACATAGTTATCATTTGCGTCCTTCGGAAGCAAAAGCGCTTTCACAAGCGGATATTGTGTTTTGGGTTGGT
GAAGGGTTAACGCCTTGGCTTGAAAAACCGTTATCAAGCTTAGCGGGTTCAGCGACTCAAATAGAAATGATAAAAGTAGA
AGGAGTAACTCTTTACGACTTTAGAGAAGGAGCAACATTTGAAGCTCATGAGCACCATGATGACGCTCGAAATGATCATG
AAGAGGAGCATAATGAACACAGCAAAGGTCATGAACACCATCACCATGATGGACATGATCCACATGTATGGTTAGACCCG
AAAAATGCTGAAGTTTGGGTGAATATAATTGCTAAAACGTTATCTGATGCAGACAGCTCAAATGCGCCAATTTATCAAAA
GAACGCTAAAATCACAGTCAGTCGTTTACATAAACTTTCTAGTAAGATTAAGCAACAAGCTAAAGGTTTAGAAGGTGTTA
AGTTCATTGTGTTTCATGATGCTTATCAATACTTTGAGAAACGCTTTCAACTATTAGCATCGGGTGCGATTTCTATTTCT
GATGCATCTAAACCGAGCCCTGCTCGAATTACAGACATTCGTGATACGGTGAAAAAGTTAGGTGTGACATGTGTCTTTAC
TGAACCTCAATATAACCCCGAGTTAGTGAATAGTATTTTTGATAACTCAACAGTAAAGACAATTGGTACTATGGATCCAC
TCGGCGCTAATTTGGCAATAGGCAAAGATCAATATGACTCATTATTGATGTCCATGATAGAGAGTCTAAGTCAGTGTAAA
CCGTGA

Upstream 100 bases:

>100_bases
TTTCAATATGGCTATTTAATTGTTATGTTATAACATAACAATTAATTTTGCCAACCTTGGTGTTTTGAAGGTTTCAAGTT
ATTACTATTAGGAGAAGAAT

Downstream 100 bases:

>100_bases
TATTTGTTTCTTACCAATAAAAACGGCAACCTAAAATAAGGTTGCCGTTTTTTATTTATCTCTTTAGAACCATGAAATTA
AATTCCTGCCGGTTTAAAGA

Product: high-affinity zinc uptake system protein ZnuA2

Products: Zn (II) [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MFKKAISVFVMSLAVVSQSYAQAPKVVVDIAPLHSLVTQVMDGVGEPDLLIRPEMSPHSYHLRPSEAKALSQADIVFWVG
EGLTPWLEKPLSSLAGSATQIEMIKVEGVTLYDFREGATFEAHEHHDDARNDHEEEHNEHSKGHEHHHHDGHDPHVWLDP
KNAEVWVNIIAKTLSDADSSNAPIYQKNAKITVSRLHKLSSKIKQQAKGLEGVKFIVFHDAYQYFEKRFQLLASGAISIS
DASKPSPARITDIRDTVKKLGVTCVFTEPQYNPELVNSIFDNSTVKTIGTMDPLGANLAIGKDQYDSLLMSMIESLSQCK
P

Sequences:

>Translated_321_residues
MFKKAISVFVMSLAVVSQSYAQAPKVVVDIAPLHSLVTQVMDGVGEPDLLIRPEMSPHSYHLRPSEAKALSQADIVFWVG
EGLTPWLEKPLSSLAGSATQIEMIKVEGVTLYDFREGATFEAHEHHDDARNDHEEEHNEHSKGHEHHHHDGHDPHVWLDP
KNAEVWVNIIAKTLSDADSSNAPIYQKNAKITVSRLHKLSSKIKQQAKGLEGVKFIVFHDAYQYFEKRFQLLASGAISIS
DASKPSPARITDIRDTVKKLGVTCVFTEPQYNPELVNSIFDNSTVKTIGTMDPLGANLAIGKDQYDSLLMSMIESLSQCK
P
>Mature_321_residues
MFKKAISVFVMSLAVVSQSYAQAPKVVVDIAPLHSLVTQVMDGVGEPDLLIRPEMSPHSYHLRPSEAKALSQADIVFWVG
EGLTPWLEKPLSSLAGSATQIEMIKVEGVTLYDFREGATFEAHEHHDDARNDHEEEHNEHSKGHEHHHHDGHDPHVWLDP
KNAEVWVNIIAKTLSDADSSNAPIYQKNAKITVSRLHKLSSKIKQQAKGLEGVKFIVFHDAYQYFEKRFQLLASGAISIS
DASKPSPARITDIRDTVKKLGVTCVFTEPQYNPELVNSIFDNSTVKTIGTMDPLGANLAIGKDQYDSLLMSMIESLSQCK
P

Specific function: Involved in the high-affinity zinc uptake transport system. Required for survival and normal growth under low Zn (2+) concentrations. Also required for virulence and intracellular growth in macrophages [H]

COG id: COG4531

COG function: function code P; ABC-type Zn2+ transport system, periplasmic component/surface adhesin

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 9 family [H]

Homologues:

Organism=Escherichia coli, GI87081990, Length=294, Percent_Identity=31.2925170068027, Blast_Score=154, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006127 [H]

Pfam domain/function: PF01297 SBP_bac_9 [H]

EC number: NA

Molecular weight: Translated: 35668; Mature: 35668

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKKAISVFVMSLAVVSQSYAQAPKVVVDIAPLHSLVTQVMDGVGEPDLLIRPEMSPHSY
CHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCEEECCCCCCCCE
HLRPSEAKALSQADIVFWVGEGLTPWLEKPLSSLAGSATQIEMIKVEGVTLYDFREGATF
ECCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCEEEEECCCCCCC
EAHEHHDDARNDHEEEHNEHSKGHEHHHHDGHDPHVWLDPKNAEVWVNIIAKTLSDADSS
CCHHCCCHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCC
NAPIYQKNAKITVSRLHKLSSKIKQQAKGLEGVKFIVFHDAYQYFEKRFQLLASGAISIS
CCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEC
DASKPSPARITDIRDTVKKLGVTCVFTEPQYNPELVNSIFDNSTVKTIGTMDPLGANLAI
CCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCCCEEE
GKDQYDSLLMSMIESLSQCKP
CCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFKKAISVFVMSLAVVSQSYAQAPKVVVDIAPLHSLVTQVMDGVGEPDLLIRPEMSPHSY
CHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCEEECCCCCCCCE
HLRPSEAKALSQADIVFWVGEGLTPWLEKPLSSLAGSATQIEMIKVEGVTLYDFREGATF
ECCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCEEEEECCCCCCC
EAHEHHDDARNDHEEEHNEHSKGHEHHHHDGHDPHVWLDPKNAEVWVNIIAKTLSDADSS
CCHHCCCHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCC
NAPIYQKNAKITVSRLHKLSSKIKQQAKGLEGVKFIVFHDAYQYFEKRFQLLASGAISIS
CCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEEC
DASKPSPARITDIRDTVKKLGVTCVFTEPQYNPELVNSIFDNSTVKTIGTMDPLGANLAI
CCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCCCEEE
GKDQYDSLLMSMIESLSQCKP
CCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Zn (II) [Periplasm]; H2O; ATP [C]

Specific reaction: Zn (II) [Periplasm] + H2O + ATP = Zn (II) [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA