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The map label for this gene is dnaG [H]

Identifier: 172087762

GI number: 172087762

Start: 2526476

End: 2528239

Strand: Direct

Name: dnaG [H]

Synonym: VF_2252

Alternate gene names: 172087762

Gene position: 2526476-2528239 (Clockwise)

Preceding gene: 59712858

Following gene: 59712861

Centisome position: 87.19

GC content: 41.21

Gene sequence:

>1764_bases
ATGGCTGGACATATCCCTCGCAGTTTTATTGATGAGCTTTTAAATCGACACGATATTGTCGATATTATTGACGCAAGAGT
AAAACTGAAAAAACAAGGGAAAAACTATGGTGCCTGCTGTCCATTTCATAATGAAAAATCCCCTTCTTTCTCTGTAAGCC
AAGAAAAACAATTTTATCACTGCTTCGGGTGTGGTGTTCATGGTAACGCCATTGACTTCCTTATGGAGTTTGACCGATTA
GAATTTGTTGAAGCTATCGAAGAACTTGCATCTATGCTTGGTCTTGATGTGCCTCGCGAACAACGCAGTGGTTTTAATGC
CCCATCATCAGGTCCAACTGCAAATTCAGAACAAAAACGCAGTTTATATGACTTAATGGGCGGCATTAGTCAGTTTTATC
GCTCCCAATTAAAATCCGCAGCAAACATTCACGCAATCAACTATCTTAAAGATAGAGGTCTCTCTGGTGAGATTGTCCAA
AAATTTGGTATTGGTTATATTGCTGATGAATGGGAGTTAGTTAAAAATAACTTTGGCCGCGATAAAGCATCACAAGATGC
TCTTGTTAGCGGCGGGATGTTAATTGAAAGCGATAATGGGCGCCGTTACGATCGTTTCCGTGGCCGAGTCATGTTCCCGA
TCAGAGATCGCCGTGGTCGCGTTATTGGCTTCGGTGGACGCGTAATTGGTGAAGGTACTCCCAAATATTTAAATTCACCT
GAAACGCCTATTTTCCATAAAGGCAAAGAGCTTTATGGTTTATATGAAGCATTACAAGCCTACAAAGACTTACCACAAGC
CTTAGTTGTTGAAGGTTATATGGATGTAGTTGCCTTGGCTCAATATGGTGTCGACTATGCCGTTGCCTCTCTAGGAACAG
CAACAACAGGCGATCATATGCAGCTTCTTTTCCGCCCAACCAGTACTGTTGTTTGTTGTTATGACGGTGACCGAGCTGGA
CGCGAAGCGGCATGGCGAGCGATGGAGCAAGCGCTTCCCTATTTAAATGATGGGCGCCAACTTAAATTTATGTTCTTACC
TGATGGTGAAGATCCAGATACTTACATCCGTCAATATGGAAAAGAAGCATTCGAACAAGAAGTCGCTAACGCTGAATCAC
TAATCAACTTCATGTTTAGAACATTGGTTGACCAAATCGATACCAGTACACGTGAAGGGAAAGCAAAATTAACAACGCTT
GCAGTGCCACTAATAGATAAAGTACCGGGTGGCACATTACGCCTTTATTTACGTGAGCAACTTGGCAAAACGCTTGGAAT
TCTCGATGAAAGTCAATTACAGCAACTAATATCTAAACAAGGTGCTGTAGAGCCCAAACGTATCGCACAACCAGAAATAA
AACGCACGGCTATGCGAGAAGTAATAACTTTATTATTACAAAACCCTGAACTTGCTCAATTAGTACCAGATTTAGAAAGT
GTTAAAGAAATTCCACTTCCTGGATTAAGTTTATTTCTTCAGCTACTTGAAAGTTGTCGACATAATCCCCATATCACCAC
AGGCCTGTTATTAGAACATTGGCGCGGTGATAAAAATGAAAAACTATTATCACGCTTGGCAGCATGGGAACTTCCTATAG
ACCAAGACAATATACAAGATGTATTTATGGATTCACTGGACAGTGTTTTAGCCCAGTGCATCAATCAACAAATTGAAAGT
TTGCAGACGAAAGAAAGAACACTTGGCTTATCAGTCGAAGAAAAAAGGGAGCTGCTAGCCTTAATGCTAGAATTAAAAGC
GTAG

Upstream 100 bases:

>100_bases
ATTAGTTAAATCTAAACTGGCTTAATAATACTCTATTTATAAAACAAGCCGTGCTATCTTTGATTGCACGGCTTGTTTGT
ATCTACAGGAAAAAGAAAAA

Downstream 100 bases:

>100_bases
AACCCAAATTGTCGTCATCAATGAAAAAATTTGTTATTATTAGTGGTTTGCATATCGCATACTAATTCCGTCACACAGAC
CAGAAGTAGGATACCGTCTA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MAGHIPRSFIDELLNRHDIVDIIDARVKLKKQGKNYGACCPFHNEKSPSFSVSQEKQFYHCFGCGVHGNAIDFLMEFDRL
EFVEAIEELASMLGLDVPREQRSGFNAPSSGPTANSEQKRSLYDLMGGISQFYRSQLKSAANIHAINYLKDRGLSGEIVQ
KFGIGYIADEWELVKNNFGRDKASQDALVSGGMLIESDNGRRYDRFRGRVMFPIRDRRGRVIGFGGRVIGEGTPKYLNSP
ETPIFHKGKELYGLYEALQAYKDLPQALVVEGYMDVVALAQYGVDYAVASLGTATTGDHMQLLFRPTSTVVCCYDGDRAG
REAAWRAMEQALPYLNDGRQLKFMFLPDGEDPDTYIRQYGKEAFEQEVANAESLINFMFRTLVDQIDTSTREGKAKLTTL
AVPLIDKVPGGTLRLYLREQLGKTLGILDESQLQQLISKQGAVEPKRIAQPEIKRTAMREVITLLLQNPELAQLVPDLES
VKEIPLPGLSLFLQLLESCRHNPHITTGLLLEHWRGDKNEKLLSRLAAWELPIDQDNIQDVFMDSLDSVLAQCINQQIES
LQTKERTLGLSVEEKRELLALMLELKA

Sequences:

>Translated_587_residues
MAGHIPRSFIDELLNRHDIVDIIDARVKLKKQGKNYGACCPFHNEKSPSFSVSQEKQFYHCFGCGVHGNAIDFLMEFDRL
EFVEAIEELASMLGLDVPREQRSGFNAPSSGPTANSEQKRSLYDLMGGISQFYRSQLKSAANIHAINYLKDRGLSGEIVQ
KFGIGYIADEWELVKNNFGRDKASQDALVSGGMLIESDNGRRYDRFRGRVMFPIRDRRGRVIGFGGRVIGEGTPKYLNSP
ETPIFHKGKELYGLYEALQAYKDLPQALVVEGYMDVVALAQYGVDYAVASLGTATTGDHMQLLFRPTSTVVCCYDGDRAG
REAAWRAMEQALPYLNDGRQLKFMFLPDGEDPDTYIRQYGKEAFEQEVANAESLINFMFRTLVDQIDTSTREGKAKLTTL
AVPLIDKVPGGTLRLYLREQLGKTLGILDESQLQQLISKQGAVEPKRIAQPEIKRTAMREVITLLLQNPELAQLVPDLES
VKEIPLPGLSLFLQLLESCRHNPHITTGLLLEHWRGDKNEKLLSRLAAWELPIDQDNIQDVFMDSLDSVLAQCINQQIES
LQTKERTLGLSVEEKRELLALMLELKA
>Mature_586_residues
AGHIPRSFIDELLNRHDIVDIIDARVKLKKQGKNYGACCPFHNEKSPSFSVSQEKQFYHCFGCGVHGNAIDFLMEFDRLE
FVEAIEELASMLGLDVPREQRSGFNAPSSGPTANSEQKRSLYDLMGGISQFYRSQLKSAANIHAINYLKDRGLSGEIVQK
FGIGYIADEWELVKNNFGRDKASQDALVSGGMLIESDNGRRYDRFRGRVMFPIRDRRGRVIGFGGRVIGEGTPKYLNSPE
TPIFHKGKELYGLYEALQAYKDLPQALVVEGYMDVVALAQYGVDYAVASLGTATTGDHMQLLFRPTSTVVCCYDGDRAGR
EAAWRAMEQALPYLNDGRQLKFMFLPDGEDPDTYIRQYGKEAFEQEVANAESLINFMFRTLVDQIDTSTREGKAKLTTLA
VPLIDKVPGGTLRLYLREQLGKTLGILDESQLQQLISKQGAVEPKRIAQPEIKRTAMREVITLLLQNPELAQLVPDLESV
KEIPLPGLSLFLQLLESCRHNPHITTGLLLEHWRGDKNEKLLSRLAAWELPIDQDNIQDVFMDSLDSVLAQCINQQIESL
QTKERTLGLSVEEKRELLALMLELKA

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=585, Percent_Identity=58.2905982905983, Blast_Score=697, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 66015; Mature: 65884

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGHIPRSFIDELLNRHDIVDIIDARVKLKKQGKNYGACCPFHNEKSPSFSVSQEKQFYH
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCEEE
CFGCGVHGNAIDFLMEFDRLEFVEAIEELASMLGLDVPREQRSGFNAPSSGPTANSEQKR
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCHHHH
SLYDLMGGISQFYRSQLKSAANIHAINYLKDRGLSGEIVQKFGIGYIADEWELVKNNFGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHCCCCC
DKASQDALVSGGMLIESDNGRRYDRFRGRVMFPIRDRRGRVIGFGGRVIGEGTPKYLNSP
CCCCCCHHHCCCEEEECCCCCHHHHHCCEEEEEEECCCCCEEEECCEEECCCCCHHCCCC
ETPIFHKGKELYGLYEALQAYKDLPQALVVEGYMDVVALAQYGVDYAVASLGTATTGDHM
CCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCE
QLLFRPTSTVVCCYDGDRAGREAAWRAMEQALPYLNDGRQLKFMFLPDGEDPDTYIRQYG
EEEEECCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHH
KEAFEQEVANAESLINFMFRTLVDQIDTSTREGKAKLTTLAVPLIDKVPGGTLRLYLREQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEHHHHHCCCCCHHHHHHHHH
LGKTLGILDESQLQQLISKQGAVEPKRIAQPEIKRTAMREVITLLLQNPELAQLVPDLES
HHHHHCCCCHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCHHH
VKEIPLPGLSLFLQLLESCRHNPHITTGLLLEHWRGDKNEKLLSRLAAWELPIDQDNIQD
HHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHH
VFMDSLDSVLAQCINQQIESLQTKERTLGLSVEEKRELLALMLELKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AGHIPRSFIDELLNRHDIVDIIDARVKLKKQGKNYGACCPFHNEKSPSFSVSQEKQFYH
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCEEE
CFGCGVHGNAIDFLMEFDRLEFVEAIEELASMLGLDVPREQRSGFNAPSSGPTANSEQKR
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCHHHH
SLYDLMGGISQFYRSQLKSAANIHAINYLKDRGLSGEIVQKFGIGYIADEWELVKNNFGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHCCCCC
DKASQDALVSGGMLIESDNGRRYDRFRGRVMFPIRDRRGRVIGFGGRVIGEGTPKYLNSP
CCCCCCHHHCCCEEEECCCCCHHHHHCCEEEEEEECCCCCEEEECCEEECCCCCHHCCCC
ETPIFHKGKELYGLYEALQAYKDLPQALVVEGYMDVVALAQYGVDYAVASLGTATTGDHM
CCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCE
QLLFRPTSTVVCCYDGDRAGREAAWRAMEQALPYLNDGRQLKFMFLPDGEDPDTYIRQYG
EEEEECCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHH
KEAFEQEVANAESLINFMFRTLVDQIDTSTREGKAKLTTLAVPLIDKVPGGTLRLYLREQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEHHHHHCCCCCHHHHHHHHH
LGKTLGILDESQLQQLISKQGAVEPKRIAQPEIKRTAMREVITLLLQNPELAQLVPDLES
HHHHHCCCCHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCHHH
VKEIPLPGLSLFLQLLESCRHNPHITTGLLLEHWRGDKNEKLLSRLAAWELPIDQDNIQD
HHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHH
VFMDSLDSVLAQCINQQIESLQTKERTLGLSVEEKRELLALMLELKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]