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The map label for this gene is yebU [H]

Identifier: 172087696

GI number: 172087696

Start: 1414112

End: 1415479

Strand: Reverse

Name: yebU [H]

Synonym: VF_1274

Alternate gene names: 172087696

Gene position: 1415479-1414112 (Counterclockwise)

Preceding gene: 59711882

Following gene: 59711880

Centisome position: 48.85

GC content: 41.89

Gene sequence:

>1368_bases
ATGCCTTCCTACTTATCAATGGATGAGTTTATTGCCGCTTGTAAACGACCTTTACGTCGTAGTATTCGTGTGAATATACT
AAAAAACAGTGTTGAAGAATTTAAACAACGTGCCGCTGAAAAACAATGGGATTTAGAACCTGTGCCTTGGTGCGATACTG
GTTTTTGGATCACGCGTCCTGAGAGCGACACAGTAAAACTAGGTAGCACCGCAGAACATATGGCTGGCCTATTCTACATT
CAAGAAGCAAGCTCTATGATGCCAGTTACCGCATTATTAAAAGATAATGATGATATCGAAATGGCTCTCGATATGGCTTC
AGCTCCCGGTTCTAAAACCACACAACTAGCTGCAGGAATGAAAAACCAAGGCGCTCTCGTTGCTAACGAATTTTCATCAA
GCCGTGTAAAAGTGCTTTGCTCAAATATACAACGCTGTGGTGTAAGCAATGTTGCTCTAACCCACTTTGATGGTCGTGTT
TTTGGTGGTTGGTTGCCAGAAACTTTTGACTCAATCTTATTGGATGCACCTTGTTCTGGCGAAGGTACCATTCGTAAAGA
CCCAGATGCAATGCATAACTGGAGCCCTGAATCAGTTATCGAAATTGGTGACACTCAACGTGATTTAATTGAAAGTGCGT
TTCATGCCCTAAAACTGGGCGGCGTAATGGTGTACTCAACCTGTACATTAAACCACGAAGAAAACCAAAACATTTGCCAT
CATCTTGTTGAACAATTTGGTGACGCCGTCACGTTTGAGCCGCTAGGTGACTTATTTGAGAGTGCAGAAAAAGCGTTAAC
CAAAGAAGGCTTCTTACATATCTACCCTCAGATTTTTGATAGTGAAGGTTTCTTTGTTGCTAAGATCCGAAAAAATAGCT
CAACCACGCCTCCAGAGGTGAAAAAACGCTTAGGCAAATTCCCTTTCGCACCGGCTTCAAATAAAGAAGCACAAGCTATT
GAAGCAGAATTAAAATCAACATTACAATTAGCAATCCCTGAAGATAATGAATTTTGGATCCGAGATAAAGAAGTCTGGGC
TTTCCCTAAACGCATGAAGCCATTGATTGGTGAAATGCGCTACCATCGAATTGGATTTAAACTTGCTGAAACCCATAAAA
AAGGATATCGCTGGCAACATGAAGCTATCATGGCACTCGCGACAGCCGATAACCCGACTTGTGCTGAACTAAATACAGAA
CAAGCACGTGAATGGTATATGGGACGAGATGTACGTCCGGACTTTTCAGGAAAAGGCGAAACGATTGTTACTTATAAAGG
CGCAGTTATTGGCTTAGGAAAATGGGTGGGTAACCGAATCAAAAATGGATTACCTCGTGAACTTGTTCGTGATGGAAACT
TGTTTTAA

Upstream 100 bases:

>100_bases
TAGCGTTTACACTTCCTGCCATTACATCTTCCAATGAGATTCATCTGTGCACGATAATATTTTTCTTCCTGACGCTTTCT
TGGCACAAGTACAAGAGACC

Downstream 100 bases:

>100_bases
ATAATACGTTTTTAAATTAGTGCCAATCTAAGTAAATCGTAAATACACTTAGATTGGTACCATACATCAAGCTGTAGGAA
TAAGGATATGCCTTCACAAT

Product: rRNA (cytosine-C(5)-)-methyltransferase RsmF

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MPSYLSMDEFIAACKRPLRRSIRVNILKNSVEEFKQRAAEKQWDLEPVPWCDTGFWITRPESDTVKLGSTAEHMAGLFYI
QEASSMMPVTALLKDNDDIEMALDMASAPGSKTTQLAAGMKNQGALVANEFSSSRVKVLCSNIQRCGVSNVALTHFDGRV
FGGWLPETFDSILLDAPCSGEGTIRKDPDAMHNWSPESVIEIGDTQRDLIESAFHALKLGGVMVYSTCTLNHEENQNICH
HLVEQFGDAVTFEPLGDLFESAEKALTKEGFLHIYPQIFDSEGFFVAKIRKNSSTTPPEVKKRLGKFPFAPASNKEAQAI
EAELKSTLQLAIPEDNEFWIRDKEVWAFPKRMKPLIGEMRYHRIGFKLAETHKKGYRWQHEAIMALATADNPTCAELNTE
QAREWYMGRDVRPDFSGKGETIVTYKGAVIGLGKWVGNRIKNGLPRELVRDGNLF

Sequences:

>Translated_455_residues
MPSYLSMDEFIAACKRPLRRSIRVNILKNSVEEFKQRAAEKQWDLEPVPWCDTGFWITRPESDTVKLGSTAEHMAGLFYI
QEASSMMPVTALLKDNDDIEMALDMASAPGSKTTQLAAGMKNQGALVANEFSSSRVKVLCSNIQRCGVSNVALTHFDGRV
FGGWLPETFDSILLDAPCSGEGTIRKDPDAMHNWSPESVIEIGDTQRDLIESAFHALKLGGVMVYSTCTLNHEENQNICH
HLVEQFGDAVTFEPLGDLFESAEKALTKEGFLHIYPQIFDSEGFFVAKIRKNSSTTPPEVKKRLGKFPFAPASNKEAQAI
EAELKSTLQLAIPEDNEFWIRDKEVWAFPKRMKPLIGEMRYHRIGFKLAETHKKGYRWQHEAIMALATADNPTCAELNTE
QAREWYMGRDVRPDFSGKGETIVTYKGAVIGLGKWVGNRIKNGLPRELVRDGNLF
>Mature_454_residues
PSYLSMDEFIAACKRPLRRSIRVNILKNSVEEFKQRAAEKQWDLEPVPWCDTGFWITRPESDTVKLGSTAEHMAGLFYIQ
EASSMMPVTALLKDNDDIEMALDMASAPGSKTTQLAAGMKNQGALVANEFSSSRVKVLCSNIQRCGVSNVALTHFDGRVF
GGWLPETFDSILLDAPCSGEGTIRKDPDAMHNWSPESVIEIGDTQRDLIESAFHALKLGGVMVYSTCTLNHEENQNICHH
LVEQFGDAVTFEPLGDLFESAEKALTKEGFLHIYPQIFDSEGFFVAKIRKNSSTTPPEVKKRLGKFPFAPASNKEAQAIE
AELKSTLQLAIPEDNEFWIRDKEVWAFPKRMKPLIGEMRYHRIGFKLAETHKKGYRWQHEAIMALATADNPTCAELNTEQ
AREWYMGRDVRPDFSGKGETIVTYKGAVIGLGKWVGNRIKNGLPRELVRDGNLF

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=306, Percent_Identity=34.3137254901961, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI76150623, Length=306, Percent_Identity=34.3137254901961, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI11545785, Length=230, Percent_Identity=30.8695652173913, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI32698918, Length=239, Percent_Identity=31.7991631799163, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI301336155, Length=254, Percent_Identity=27.9527559055118, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI39995082, Length=276, Percent_Identity=27.536231884058, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI23199998, Length=230, Percent_Identity=30.8695652173913, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI270288816, Length=230, Percent_Identity=30.8695652173913, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI8922322, Length=230, Percent_Identity=30.8695652173913, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI270288818, Length=230, Percent_Identity=30.8695652173913, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI40316918, Length=187, Percent_Identity=31.0160427807487, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI87081985, Length=457, Percent_Identity=59.5185995623632, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI2367212, Length=237, Percent_Identity=29.957805907173, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17536757, Length=310, Percent_Identity=33.5483870967742, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI25143471, Length=274, Percent_Identity=28.8321167883212, Blast_Score=92, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324268, Length=300, Percent_Identity=36, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319447, Length=307, Percent_Identity=25.0814332247557, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI22024126, Length=304, Percent_Identity=33.2236842105263, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI21355201, Length=205, Percent_Identity=31.7073170731707, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24668781, Length=181, Percent_Identity=32.0441988950276, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356513, Length=178, Percent_Identity=33.7078651685393, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 51057; Mature: 50925

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSYLSMDEFIAACKRPLRRSIRVNILKNSVEEFKQRAAEKQWDLEPVPWCDTGFWITRP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECC
ESDTVKLGSTAEHMAGLFYIQEASSMMPVTALLKDNDDIEMALDMASAPGSKTTQLAAGM
CCCCEECCCCHHHHHHHHEEECCCCCCCEEEEECCCCCCEEEEECCCCCCCCHHHHHCCC
KNQGALVANEFSSSRVKVLCSNIQRCGVSNVALTHFDGRVFGGWLPETFDSILLDAPCSG
CCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCEECCCCHHHHHHHEECCCCCC
EGTIRKDPDAMHNWSPESVIEIGDTQRDLIESAFHALKLGGVMVYSTCTLNHEENQNICH
CCCCCCCCHHHHCCCCCHHEECCCCHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHH
HLVEQFGDAVTFEPLGDLFESAEKALTKEGFLHIYPQIFDSEGFFVAKIRKNSSTTPPEV
HHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCEEECHHHCCCCCEEEEEEECCCCCCCHHH
KKRLGKFPFAPASNKEAQAIEAELKSTLQLAIPEDNEFWIRDKEVWAFPKRMKPLIGEMR
HHHHCCCCCCCCCCCHHHHHHHHHHHHEEEECCCCCCEEEECCHHHHCHHHHHHHHHHHH
YHRIGFKLAETHKKGYRWQHEAIMALATADNPTCAELNTEQAREWYMGRDVRPDFSGKGE
HHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
TIVTYKGAVIGLGKWVGNRIKNGLPRELVRDGNLF
EEEEECCCEEEHHHHHHHHHHCCCCHHHHHCCCCC
>Mature Secondary Structure 
PSYLSMDEFIAACKRPLRRSIRVNILKNSVEEFKQRAAEKQWDLEPVPWCDTGFWITRP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECC
ESDTVKLGSTAEHMAGLFYIQEASSMMPVTALLKDNDDIEMALDMASAPGSKTTQLAAGM
CCCCEECCCCHHHHHHHHEEECCCCCCCEEEEECCCCCCEEEEECCCCCCCCHHHHHCCC
KNQGALVANEFSSSRVKVLCSNIQRCGVSNVALTHFDGRVFGGWLPETFDSILLDAPCSG
CCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCEECCCCHHHHHHHEECCCCCC
EGTIRKDPDAMHNWSPESVIEIGDTQRDLIESAFHALKLGGVMVYSTCTLNHEENQNICH
CCCCCCCCHHHHCCCCCHHEECCCCHHHHHHHHHHHHHHCCEEEEEEEEECCCCCHHHHH
HLVEQFGDAVTFEPLGDLFESAEKALTKEGFLHIYPQIFDSEGFFVAKIRKNSSTTPPEV
HHHHHHCCEEEEHHHHHHHHHHHHHHHHCCCEEECHHHCCCCCEEEEEEECCCCCCCHHH
KKRLGKFPFAPASNKEAQAIEAELKSTLQLAIPEDNEFWIRDKEVWAFPKRMKPLIGEMR
HHHHCCCCCCCCCCCHHHHHHHHHHHHEEEECCCCCCEEEECCHHHHCHHHHHHHHHHHH
YHRIGFKLAETHKKGYRWQHEAIMALATADNPTCAELNTEQAREWYMGRDVRPDFSGKGE
HHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
TIVTYKGAVIGLGKWVGNRIKNGLPRELVRDGNLF
EEEEECCCEEEHHHHHHHHHHCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA