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The map label for this gene is thrS [H]

Identifier: 172087694

GI number: 172087694

Start: 1349715

End: 1351643

Strand: Direct

Name: thrS [H]

Synonym: VF_1214

Alternate gene names: 172087694

Gene position: 1349715-1351643 (Clockwise)

Preceding gene: 59711817

Following gene: 59711823

Centisome position: 46.58

GC content: 42.41

Gene sequence:

>1929_bases
ATGCCTGTAATTACTCTTCCAGACGGTTCTCAACGTCAATTCGATAACGCTGTTTCAACTATGGATGTCGCTGCTGACAT
CGGTCCAGGTCTTGCGAAAGCTTGTATCGCTGGTCGTGTTGATGGTGTTCGTGTTGATGCGTGCGATTTAATTGAAAATG
ACGCTCAACTTGAAATCATCACAGCAAAAGATGAAGACGGTTTAGAAATCATTCGTCACTCTTGTGCGCACCTTTTAGGT
CACGCAGTTAAGCAGCTTTTCCCTGAAGCTAAGATGGCGATTGGTCCAACAATCGATAACGGTTTCTACTACGATATCGA
TATGGAGCACTCTTTAACGCAAGAAGATCTTGATAAGATTGAAAAGCGTATGAAGGATCTTGCTAAGACCAAGTACCAAG
TAATTAAGAAGAACGTAAGCTGGCAAGAAGCGCGTGATACTTTTGAAGCACGCGGTGAAACTTACAAGATTGAAATCCTT
GACGAAAACGTATCTAAAGACGATCGTCCAGGTTTATATCACCACGAAGAATACATTGATATGTGTCGTGGTCCTCACGT
TCCTAACATGAGCTTCTGTCAGAACTTTAAGATTCTGAACGTTGCTGGTGCATACTGGCGTGGTAACAGTGACAACAAAA
TGCTGCAACGTGTCTACGGCACTGCATTCCAAGATAAGAAATTGCTTAAAGCACACCTTATCCGCCTAGAAGAAGCAGCA
AAGCGTGATCACCGTAAAATTGGTAAGCAACTTGATCTGTTCCATATGCAGCAAGAAGCTCCAGGTATGGTGTTCTGGCA
CCACAACGGTTGGACTATCTTCCGTGAACTAGAAGTATTTGTTCGTGAAAAACTAACTCAGTACGATTACCAAGAAGTAA
AAGGCCCATTAATGATGGACCGCGTACTTTGGGAACGTTCTGGTCACTGGGACAAATACGCTGAAGCGATGTTCACAACA
AGTTCTGAGAACCGTGAATACGCAATTAAACCAATGAACTGTCCAGGTCACGTTCAAATCTTTAACCAAGGTCTGAAATC
ATACCGTGATTTACCATTACGTATGGCTGAGTTTGGCTCATGTCACCGTAATGAACCATCTGGTGCTCTACACGGCATTA
TGCGTGTTCGTGGCTTCACTCAAGATGATGCACACGTATTCTGTACAGAAGCACAAGTTCAACAAGAAGTTAAATCTTGT
ATTGAAATGGTTTACGATACATATCAAACATTTGGTTTTGATAATATCGTAGTTAAGCTGTCTACACGTCCAGAACAACG
CGTTGGTTCTGATGAAATGTGGGACCGTGCTGAGTCAGATTTAAAACTTGCTCTAGAGTCAATGGAGATTCCATACGAGA
TTCAAGAGGGTGAGGGTGCGTTCTACGGACCGAAAATTGAATTTACTTTGCATGATTGTCTGGACCGTGCGTGGCAATGT
GGTACAGTACAGCTTGATTTTGCATTACCAGAGCGTTTAGGTGCAACTTACGTTGGTGAAGATAACGAACGTCACACACC
AGTGATGATTCACCGCGCGATTTTAGGTTCACTAGAGCGTTTCATTGGTATCTTAATCGAAGACTATGCAGGCTTTTTCC
CAACGTGGTTAGCTCCTGAACAAGCGATTGTTATGGGAATTACGGACAAACAAGCTGATTACGTACAAGAAATTGCAAAA
AAACTGCAAAAAAATGGATTTAGAGTGAAAGCGGACTTGAGAAATGAGAAGATTGGCTTTAAAATCCGTGAACATACTTT
GAAGCGTGTACCGTATATGCTTGTTTGTGGCGACCAGGAGATGGAAGCTGGAGAAATTGCAGTACGTACTCGTAAAGGTA
AAGATTTGGGTAAATTCAAAATTGATGATTTTGTTGCATTCCTGCAGCAAGAAGTTAGTGCCCGAACGCTCAATACTGTG
GAGGAATAA

Upstream 100 bases:

>100_bases
TTGATTGATTCTCACAACTACTCTATATACAACGTATTTTTCGTTTATTAATTTTTAGCATGTGTTGCTTCGTGTGCAAC
ACCACTGTAAAGGATAAAAC

Downstream 100 bases:

>100_bases
GGTATTAAAGGCGGAAAAAGAGCCCAACAGCCGGCTAAACAAAATGCTCATCGTCTAAACGGTGAAATTCGTGGCGTTAA
AGAAGTGCGCCTAACAGGCC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 642; Mature: 641

Protein sequence:

>642_residues
MPVITLPDGSQRQFDNAVSTMDVAADIGPGLAKACIAGRVDGVRVDACDLIENDAQLEIITAKDEDGLEIIRHSCAHLLG
HAVKQLFPEAKMAIGPTIDNGFYYDIDMEHSLTQEDLDKIEKRMKDLAKTKYQVIKKNVSWQEARDTFEARGETYKIEIL
DENVSKDDRPGLYHHEEYIDMCRGPHVPNMSFCQNFKILNVAGAYWRGNSDNKMLQRVYGTAFQDKKLLKAHLIRLEEAA
KRDHRKIGKQLDLFHMQQEAPGMVFWHHNGWTIFRELEVFVREKLTQYDYQEVKGPLMMDRVLWERSGHWDKYAEAMFTT
SSENREYAIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGALHGIMRVRGFTQDDAHVFCTEAQVQQEVKSC
IEMVYDTYQTFGFDNIVVKLSTRPEQRVGSDEMWDRAESDLKLALESMEIPYEIQEGEGAFYGPKIEFTLHDCLDRAWQC
GTVQLDFALPERLGATYVGEDNERHTPVMIHRAILGSLERFIGILIEDYAGFFPTWLAPEQAIVMGITDKQADYVQEIAK
KLQKNGFRVKADLRNEKIGFKIREHTLKRVPYMLVCGDQEMEAGEIAVRTRKGKDLGKFKIDDFVAFLQQEVSARTLNTV
EE

Sequences:

>Translated_642_residues
MPVITLPDGSQRQFDNAVSTMDVAADIGPGLAKACIAGRVDGVRVDACDLIENDAQLEIITAKDEDGLEIIRHSCAHLLG
HAVKQLFPEAKMAIGPTIDNGFYYDIDMEHSLTQEDLDKIEKRMKDLAKTKYQVIKKNVSWQEARDTFEARGETYKIEIL
DENVSKDDRPGLYHHEEYIDMCRGPHVPNMSFCQNFKILNVAGAYWRGNSDNKMLQRVYGTAFQDKKLLKAHLIRLEEAA
KRDHRKIGKQLDLFHMQQEAPGMVFWHHNGWTIFRELEVFVREKLTQYDYQEVKGPLMMDRVLWERSGHWDKYAEAMFTT
SSENREYAIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGALHGIMRVRGFTQDDAHVFCTEAQVQQEVKSC
IEMVYDTYQTFGFDNIVVKLSTRPEQRVGSDEMWDRAESDLKLALESMEIPYEIQEGEGAFYGPKIEFTLHDCLDRAWQC
GTVQLDFALPERLGATYVGEDNERHTPVMIHRAILGSLERFIGILIEDYAGFFPTWLAPEQAIVMGITDKQADYVQEIAK
KLQKNGFRVKADLRNEKIGFKIREHTLKRVPYMLVCGDQEMEAGEIAVRTRKGKDLGKFKIDDFVAFLQQEVSARTLNTV
EE
>Mature_641_residues
PVITLPDGSQRQFDNAVSTMDVAADIGPGLAKACIAGRVDGVRVDACDLIENDAQLEIITAKDEDGLEIIRHSCAHLLGH
AVKQLFPEAKMAIGPTIDNGFYYDIDMEHSLTQEDLDKIEKRMKDLAKTKYQVIKKNVSWQEARDTFEARGETYKIEILD
ENVSKDDRPGLYHHEEYIDMCRGPHVPNMSFCQNFKILNVAGAYWRGNSDNKMLQRVYGTAFQDKKLLKAHLIRLEEAAK
RDHRKIGKQLDLFHMQQEAPGMVFWHHNGWTIFRELEVFVREKLTQYDYQEVKGPLMMDRVLWERSGHWDKYAEAMFTTS
SENREYAIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGALHGIMRVRGFTQDDAHVFCTEAQVQQEVKSCI
EMVYDTYQTFGFDNIVVKLSTRPEQRVGSDEMWDRAESDLKLALESMEIPYEIQEGEGAFYGPKIEFTLHDCLDRAWQCG
TVQLDFALPERLGATYVGEDNERHTPVMIHRAILGSLERFIGILIEDYAGFFPTWLAPEQAIVMGITDKQADYVQEIAKK
LQKNGFRVKADLRNEKIGFKIREHTLKRVPYMLVCGDQEMEAGEIAVRTRKGKDLGKFKIDDFVAFLQQEVSARTLNTVE
E

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=622, Percent_Identity=39.0675241157556, Blast_Score=454, Evalue=1e-128,
Organism=Homo sapiens, GI38202255, Length=643, Percent_Identity=37.9471228615863, Blast_Score=448, Evalue=1e-126,
Organism=Homo sapiens, GI20070344, Length=662, Percent_Identity=34.2900302114804, Blast_Score=384, Evalue=1e-106,
Organism=Escherichia coli, GI1788013, Length=642, Percent_Identity=76.3239875389408, Blast_Score=1083, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=165, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71984192, Length=627, Percent_Identity=38.1180223285486, Blast_Score=457, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71984184, Length=627, Percent_Identity=38.1180223285486, Blast_Score=457, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6322112, Length=627, Percent_Identity=37.9585326953748, Blast_Score=437, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6322654, Length=398, Percent_Identity=38.1909547738693, Blast_Score=303, Evalue=5e-83,
Organism=Drosophila melanogaster, GI24583839, Length=630, Percent_Identity=39.6825396825397, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24583842, Length=630, Percent_Identity=39.6825396825397, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI28574141, Length=630, Percent_Identity=39.6825396825397, Blast_Score=474, Evalue=1e-134,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73720; Mature: 73589

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00018 EF_HAND_1 ; PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVITLPDGSQRQFDNAVSTMDVAADIGPGLAKACIAGRVDGVRVDACDLIENDAQLEII
CCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEECHHHHHCCCCEEEEE
TAKDEDGLEIIRHSCAHLLGHAVKQLFPEAKMAIGPTIDNGFYYDIDMEHSLTQEDLDKI
EECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEECCCEEEEECCCCCCCHHHHHHH
EKRMKDLAKTKYQVIKKNVSWQEARDTFEARGETYKIEILDENVSKDDRPGLYHHEEYID
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCHHHHH
MCRGPHVPNMSFCQNFKILNVAGAYWRGNSDNKMLQRVYGTAFQDKKLLKAHLIRLEEAA
HHCCCCCCCHHHHCCCEEEEECCCEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
KRDHRKIGKQLDLFHMQQEAPGMVFWHHNGWTIFRELEVFVREKLTQYDYQEVKGPLMMD
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEHHHHHHHHHHHHHHHCCHHHHCCHHHHH
RVLWERSGHWDKYAEAMFTTSSENREYAIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGS
HHHHHCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHCC
CHRNEPSGALHGIMRVRGFTQDDAHVFCTEAQVQQEVKSCIEMVYDTYQTFGFDNIVVKL
CCCCCCCHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
STRPEQRVGSDEMWDRAESDLKLALESMEIPYEIQEGEGAFYGPKIEFTLHDCLDRAWQC
ECCCHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEECCEEEEEHHHHHHHHHCC
GTVQLDFALPERLGATYVGEDNERHTPVMIHRAILGSLERFIGILIEDYAGFFPTWLAPE
CCEEEEECCHHHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
QAIVMGITDKQADYVQEIAKKLQKNGFRVKADLRNEKIGFKIREHTLKRVPYMLVCGDQE
CEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCEEEEECCCC
MEAGEIAVRTRKGKDLGKFKIDDFVAFLQQEVSARTLNTVEE
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PVITLPDGSQRQFDNAVSTMDVAADIGPGLAKACIAGRVDGVRVDACDLIENDAQLEII
CEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCEEECHHHHHCCCCEEEEE
TAKDEDGLEIIRHSCAHLLGHAVKQLFPEAKMAIGPTIDNGFYYDIDMEHSLTQEDLDKI
EECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCEECCCEEEEECCCCCCCHHHHHHH
EKRMKDLAKTKYQVIKKNVSWQEARDTFEARGETYKIEILDENVSKDDRPGLYHHEEYID
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCHHHHH
MCRGPHVPNMSFCQNFKILNVAGAYWRGNSDNKMLQRVYGTAFQDKKLLKAHLIRLEEAA
HHCCCCCCCHHHHCCCEEEEECCCEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
KRDHRKIGKQLDLFHMQQEAPGMVFWHHNGWTIFRELEVFVREKLTQYDYQEVKGPLMMD
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCEEHHHHHHHHHHHHHHHCCHHHHCCHHHHH
RVLWERSGHWDKYAEAMFTTSSENREYAIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGS
HHHHHCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHCC
CHRNEPSGALHGIMRVRGFTQDDAHVFCTEAQVQQEVKSCIEMVYDTYQTFGFDNIVVKL
CCCCCCCHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
STRPEQRVGSDEMWDRAESDLKLALESMEIPYEIQEGEGAFYGPKIEFTLHDCLDRAWQC
ECCCHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEECCEEEEEHHHHHHHHHCC
GTVQLDFALPERLGATYVGEDNERHTPVMIHRAILGSLERFIGILIEDYAGFFPTWLAPE
CCEEEEECCHHHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
QAIVMGITDKQADYVQEIAKKLQKNGFRVKADLRNEKIGFKIREHTLKRVPYMLVCGDQE
CEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCEEEEECCCC
MEAGEIAVRTRKGKDLGKFKIDDFVAFLQQEVSARTLNTVEE
CCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA