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The map label for this gene is trmB [H]

Identifier: 172087664

GI number: 172087664

Start: 452308

End: 453024

Strand: Direct

Name: trmB [H]

Synonym: VF_2589

Alternate gene names: 172087664

Gene position: 452308-453024 (Clockwise)

Preceding gene: 59711022

Following gene: 59711030

Centisome position: 15.61

GC content: 42.4

Gene sequence:

>717_bases
ATGACTGAAGTGACTAAAAATGATTTTACCGAAGAAGGTAAAATTGTTCGTAAAATCCGTAGCTTTGTTCGCCGTGAAGG
GCGTTTAACTAAAGGCCAAGAAGGTGCTATCCAAGAATGTTGGCCTAGCATGGGTATTGATTTTGTAGAGCAAATGCTTG
ATTGGAATGAAGTGTTTGGTAACAATAATCCAGTTGTTCTTGAAATCGGTTTTGGTATGGGCGCATCTCTTGTTGAGATG
GCTAAAAATGCTCCAGAGAAAAACTTCTTAGGTATCGAAGTTCACCGTCCAGGTGTTGGCGCATGTCTTGCGGCAGCAAA
AGAAGCTGGCGTAACTAACCTACGTGTTATGTGTCATGATGCAGTTGAAGTATTTGAATCAATGATCCCTGATTCATCTG
TAAATACTCTACAACTTTTCTTCCCTGACCCATGGCATAAAGCGCGTCATCATAAGCGCCGTATCGTTAAAGCTGAGTTT
GCTGAGATGATCCGTCCTAAGTTAATCCAAGGTGGTATCTTCCACATGGCAACTGATTGGGAAAATTACTCAGAGCATAT
GATTGAAGTAATGAATGCTGCACCGGGTTATGAAAACATTGCCACTGATGGTGATTTCATTCCTCGCCCAGATGAGCGTC
CATTAACTAAGTTTGAAGCTCGTGGCCACCGTTTAGGTCATGGCGTTTGGGATATTAAATTCCGTCGTAACGCATAG

Upstream 100 bases:

>100_bases
TATAATGGGAAAACGAAAGATAATACTTGAACACTGGGCTAAACTTTGGATAATGCCGGATCCACAATCCTTGTTCAGAG
AATAACCAGGCAATCCAATC

Downstream 100 bases:

>100_bases
TTAGTATTGTCGTAATACAATATAATTAAAGCCAACCTGAGTGTTGGCTTTTTTGTCCTTGCTGTCAAAAGGAAGTAGAA
ATAATGAAGCCAACAAAACA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MTEVTKNDFTEEGKIVRKIRSFVRREGRLTKGQEGAIQECWPSMGIDFVEQMLDWNEVFGNNNPVVLEIGFGMGASLVEM
AKNAPEKNFLGIEVHRPGVGACLAAAKEAGVTNLRVMCHDAVEVFESMIPDSSVNTLQLFFPDPWHKARHHKRRIVKAEF
AEMIRPKLIQGGIFHMATDWENYSEHMIEVMNAAPGYENIATDGDFIPRPDERPLTKFEARGHRLGHGVWDIKFRRNA

Sequences:

>Translated_238_residues
MTEVTKNDFTEEGKIVRKIRSFVRREGRLTKGQEGAIQECWPSMGIDFVEQMLDWNEVFGNNNPVVLEIGFGMGASLVEM
AKNAPEKNFLGIEVHRPGVGACLAAAKEAGVTNLRVMCHDAVEVFESMIPDSSVNTLQLFFPDPWHKARHHKRRIVKAEF
AEMIRPKLIQGGIFHMATDWENYSEHMIEVMNAAPGYENIATDGDFIPRPDERPLTKFEARGHRLGHGVWDIKFRRNA
>Mature_237_residues
TEVTKNDFTEEGKIVRKIRSFVRREGRLTKGQEGAIQECWPSMGIDFVEQMLDWNEVFGNNNPVVLEIGFGMGASLVEMA
KNAPEKNFLGIEVHRPGVGACLAAAKEAGVTNLRVMCHDAVEVFESMIPDSSVNTLQLFFPDPWHKARHHKRRIVKAEFA
EMIRPKLIQGGIFHMATDWENYSEHMIEVMNAAPGYENIATDGDFIPRPDERPLTKFEARGHRLGHGVWDIKFRRNA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=235, Percent_Identity=64.6808510638298, Blast_Score=338, Evalue=3e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 27020; Mature: 26889

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEVTKNDFTEEGKIVRKIRSFVRREGRLTKGQEGAIQECWPSMGIDFVEQMLDWNEVFG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHC
NNNPVVLEIGFGMGASLVEMAKNAPEKNFLGIEVHRPGVGACLAAAKEAGVTNLRVMCHD
CCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHH
AVEVFESMIPDSSVNTLQLFFPDPWHKARHHKRRIVKAEFAEMIRPKLIQGGIFHMATDW
HHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCH
ENYSEHMIEVMNAAPGYENIATDGDFIPRPDERPLTKFEARGHRLGHGVWDIKFRRNA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
TEVTKNDFTEEGKIVRKIRSFVRREGRLTKGQEGAIQECWPSMGIDFVEQMLDWNEVFG
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHC
NNNPVVLEIGFGMGASLVEMAKNAPEKNFLGIEVHRPGVGACLAAAKEAGVTNLRVMCHD
CCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHH
AVEVFESMIPDSSVNTLQLFFPDPWHKARHHKRRIVKAEFAEMIRPKLIQGGIFHMATDW
HHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCH
ENYSEHMIEVMNAAPGYENIATDGDFIPRPDERPLTKFEARGHRLGHGVWDIKFRRNA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA