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The map label for this gene is mutY [H]

Identifier: 172087663

GI number: 172087663

Start: 451011

End: 452063

Strand: Reverse

Name: mutY [H]

Synonym: VF_0422

Alternate gene names: 172087663

Gene position: 452063-451011 (Counterclockwise)

Preceding gene: 59711031

Following gene: 59711028

Centisome position: 15.6

GC content: 41.22

Gene sequence:

>1053_bases
ATGACGTCTTTCTCTCAAGCTATTTTAGAATGGTACGATAATTACGGTCGAAAAACGCTGCCTTGGCAGTTAGAAAAAAC
GCCTTATAAAGTATGGTTATCGGAGATCATGCTACAACAAACCCAAGTCACTACCGTTATCCCTTACTTCGAACGCTTCA
TGACACGATTTCCAACAATCGTTGATTTAGCACATGCCGAGCAAGATGAAGTTCTTCACTTATGGACAGGGCTAGGCTAT
TACGCTCGTGCTCGTAATTTACATAAAACGGCACAAATCATTGCTGAGCAATATAATGGTATTTTCCCTACAAATATTGA
TGATGTGATTGCACTTCCTGGTATTGGTCGCTCAACAGCCGGCGCTGTTTTATCGCTTTCTTTGCAACAGCATCATCCCA
TTCTTGATGGTAACGTTAAAAGAACATTATCTCGCTGTTTCGCAATAGAAGGCTGGCCAGGTAAAAAAAGCGTTGAAAAT
GAAATGTGGGCGGTGGCTGAAACTCATACTCCAAAGCAAGGTGTTGAGCGCTATAACCAAGCAATGATGGATATGGGAGC
AATGGTATGTACACGCTCCAAACCTAAATGTGAGTTGTGCCCAGTAAACGATCTTTGCCAAGCAAAAGCCCAAGATAAAC
AATTAGACTTTCCGACCAAAAAGCCAAAGAAAGAGAAACCAGTTAAAGAAGCGTGGTTTGCTATCTACTATCATGATGGC
GAAGTTTGGTTAGAGCAGAGACCACAAAGTGGCATTTGGGGAGGATTATTTTGTTTTCCCGAGCAACCAACAAATACACT
TGATGAGTTATCAGAAGACTATGGTTTTAAAGTAGCATCAAAACAACAGTTAATCGCTTTTCGCCATACTTTTAGCCACT
ACCATCTTGATATTACCCCTGTACTTATCACTCTTGCGAAGAAACCGAACATGATAATGGAAGGAACGCGTGGAGTTTGG
TATAACTTATCTCAACCAATGACGGTCGGGTTAGCTGCACCAGTTCAAAAATTATTGGATGCCCTACCGTACGAAATTTC
TAATGGAGAATAA

Upstream 100 bases:

>100_bases
TTACCTTCAAAGCAAATATATATCCTTACTTTCTATCGGGTGTACAAACTCATCACAGTGTGGTGCAATCTCTAGAATTA
TAAAAATGAATGAGCATGTC

Downstream 100 bases:

>100_bases
CCATGAGCCGCACTGTGTTTTGCGTTCGTTTAAATAAAGAAGCCGATGGCCTAGATTTTCAACTTTACCCAGGTGAACTA
GGTAAGCGTATTTTTGACAA

Product: adenine DNA glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MTSFSQAILEWYDNYGRKTLPWQLEKTPYKVWLSEIMLQQTQVTTVIPYFERFMTRFPTIVDLAHAEQDEVLHLWTGLGY
YARARNLHKTAQIIAEQYNGIFPTNIDDVIALPGIGRSTAGAVLSLSLQQHHPILDGNVKRTLSRCFAIEGWPGKKSVEN
EMWAVAETHTPKQGVERYNQAMMDMGAMVCTRSKPKCELCPVNDLCQAKAQDKQLDFPTKKPKKEKPVKEAWFAIYYHDG
EVWLEQRPQSGIWGGLFCFPEQPTNTLDELSEDYGFKVASKQQLIAFRHTFSHYHLDITPVLITLAKKPNMIMEGTRGVW
YNLSQPMTVGLAAPVQKLLDALPYEISNGE

Sequences:

>Translated_350_residues
MTSFSQAILEWYDNYGRKTLPWQLEKTPYKVWLSEIMLQQTQVTTVIPYFERFMTRFPTIVDLAHAEQDEVLHLWTGLGY
YARARNLHKTAQIIAEQYNGIFPTNIDDVIALPGIGRSTAGAVLSLSLQQHHPILDGNVKRTLSRCFAIEGWPGKKSVEN
EMWAVAETHTPKQGVERYNQAMMDMGAMVCTRSKPKCELCPVNDLCQAKAQDKQLDFPTKKPKKEKPVKEAWFAIYYHDG
EVWLEQRPQSGIWGGLFCFPEQPTNTLDELSEDYGFKVASKQQLIAFRHTFSHYHLDITPVLITLAKKPNMIMEGTRGVW
YNLSQPMTVGLAAPVQKLLDALPYEISNGE
>Mature_349_residues
TSFSQAILEWYDNYGRKTLPWQLEKTPYKVWLSEIMLQQTQVTTVIPYFERFMTRFPTIVDLAHAEQDEVLHLWTGLGYY
ARARNLHKTAQIIAEQYNGIFPTNIDDVIALPGIGRSTAGAVLSLSLQQHHPILDGNVKRTLSRCFAIEGWPGKKSVENE
MWAVAETHTPKQGVERYNQAMMDMGAMVCTRSKPKCELCPVNDLCQAKAQDKQLDFPTKKPKKEKPVKEAWFAIYYHDGE
VWLEQRPQSGIWGGLFCFPEQPTNTLDELSEDYGFKVASKQQLIAFRHTFSHYHLDITPVLITLAKKPNMIMEGTRGVWY
NLSQPMTVGLAAPVQKLLDALPYEISNGE

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI190358497, Length=360, Percent_Identity=33.0555555555556, Blast_Score=185, Evalue=5e-47,
Organism=Homo sapiens, GI6912520, Length=360, Percent_Identity=33.0555555555556, Blast_Score=185, Evalue=5e-47,
Organism=Homo sapiens, GI115298648, Length=360, Percent_Identity=33.0555555555556, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI115298650, Length=360, Percent_Identity=33.0555555555556, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI115298654, Length=360, Percent_Identity=33.0555555555556, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI115298652, Length=360, Percent_Identity=33.0555555555556, Blast_Score=185, Evalue=6e-47,
Organism=Escherichia coli, GI1789331, Length=342, Percent_Identity=59.9415204678363, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI1787920, Length=135, Percent_Identity=31.8518518518519, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 39972; Mature: 39841

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSFSQAILEWYDNYGRKTLPWQLEKTPYKVWLSEIMLQQTQVTTVIPYFERFMTRFPTI
CCHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
VDLAHAEQDEVLHLWTGLGYYARARNLHKTAQIIAEQYNGIFPTNIDDVIALPGIGRSTA
HHHHHCCCCCEEHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCC
GAVLSLSLQQHHPILDGNVKRTLSRCFAIEGWPGKKSVENEMWAVAETHTPKQGVERYNQ
CHHEEEEHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHEEEECCCCCHHHHHHHHH
AMMDMGAMVCTRSKPKCELCPVNDLCQAKAQDKQLDFPTKKPKKEKPVKEAWFAIYYHDG
HHHHHHHHHHCCCCCCCEECCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHEEEEEEEECC
EVWLEQRPQSGIWGGLFCFPEQPTNTLDELSEDYGFKVASKQQLIAFRHTFSHYHLDITP
EEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHCCEECCHHHHHHHHHHHHHEEEEHHH
VLITLAKKPNMIMEGTRGVWYNLSQPMTVGLAAPVQKLLDALPYEISNGE
HHHHHCCCCCEEEECCCCEEEECCCCCEEHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TSFSQAILEWYDNYGRKTLPWQLEKTPYKVWLSEIMLQQTQVTTVIPYFERFMTRFPTI
CHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
VDLAHAEQDEVLHLWTGLGYYARARNLHKTAQIIAEQYNGIFPTNIDDVIALPGIGRSTA
HHHHHCCCCCEEHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCC
GAVLSLSLQQHHPILDGNVKRTLSRCFAIEGWPGKKSVENEMWAVAETHTPKQGVERYNQ
CHHEEEEHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHEEEECCCCCHHHHHHHHH
AMMDMGAMVCTRSKPKCELCPVNDLCQAKAQDKQLDFPTKKPKKEKPVKEAWFAIYYHDG
HHHHHHHHHHCCCCCCCEECCCHHHHHHHCCCCCCCCCCCCCCCCCCHHHEEEEEEEECC
EVWLEQRPQSGIWGGLFCFPEQPTNTLDELSEDYGFKVASKQQLIAFRHTFSHYHLDITP
EEEEECCCCCCCCCCEEECCCCCCCHHHHHHHHHCCEECCHHHHHHHHHHHHHEEEEHHH
VLITLAKKPNMIMEGTRGVWYNLSQPMTVGLAAPVQKLLDALPYEISNGE
HHHHHCCCCCEEEECCCCEEEECCCCCEEHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]