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The map label for this gene is ksgA

Identifier: 170762429

GI number: 170762429

Start: 749017

End: 749850

Strand: Reverse

Name: ksgA

Synonym: UPA3_0641

Alternate gene names: 170762429

Gene position: 749850-749017 (Counterclockwise)

Preceding gene: 170762036

Following gene: 170762347

Centisome position: 99.76

GC content: 22.66

Gene sequence:

>834_bases
TTGAATAAATCATTCATTAAAAATAAACTCAAGCAAGAAAGTTTTGTTCCATCAAAAAAAATGGGGCAAAACTTCTTATT
GTCTAATAACATAAAAAATAAAATTGTAGATGTGGCGAATATTAATAAAGATGATCTTATATTAGAAATTGGTCCAGGGT
GAGGAGCAATTACTGAAATTCTTGTACAAAAAACAAATATTTTAATTGCTATTGAATTAGATAAAAGACTATATGCACAC
TTAAAAACATACATAAAAACAAGTAATTTTCATATAATAAATAATGATGTATTATGTGTAGATTTAGATAATTTGATTTT
AGATTACAATAATACTCAAAAAATTCAAAAAATTAAAGTTGTTGCAAATCTTCCATATGCAATATCTTCAAAGATAGTTT
TAAAAATTATTCAATCAAAATTAATTAATGATGCTTATATTATGGTTCAAAAAGAAATGGCTGAACGAATTGGTGCTAAA
GTAAATACACGTGGTTATAATGCTTTTACTGTTTTAGTACAATTATTCTGTAAAACAAAAATTCTATTCGAAGTTAATGC
TAAAGAATTTCATCCCCAACCTAAAGTTCAAAGTGCTGTCATTCATCTTGAAAATCTTCATAATAGTGTGAATTTTAATA
TTGAAGAATTAGGTAAATTTTTACGGATTTGTTTTTTAAATAAACGAAAAAAGTTAAAAAATAACTTATCTAATATTTAT
GATATTAAAATAATTAATCAAATGTTTATTGATTATAATTTAGATATGAATTTACGAGCTGAAAACATTGAACCAAAAAT
GTTTCTAAAACTCTTTAATTATTTGAATAGATAA

Upstream 100 bases:

>100_bases
ATTCTGCAATCGTATATCATTCATGTTATTATTATGAAACGCCGTTCAAACAGTGCCACAAGAATTGAATCAAAACTTGC
TAAATTAGGAATTAGCTAAC

Downstream 100 bases:

>100_bases
GATAATTAATAAAATTAAGTTGTAGGTCAAATTATGAAAGTAAAGTCATTCGCAAAAATTGATTTAGGATATAGTGTTTA
TAAAAAACAAAAGAATTTTA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MNKSFIKNKLKQESFVPSKKMGQNFLLSNNIKNKIVDVANINKDDLILEIGPGWGAITEILVQKTNILIAIELDKRLYAH
LKTYIKTSNFHIINNDVLCVDLDNLILDYNNTQKIQKIKVVANLPYAISSKIVLKIIQSKLINDAYIMVQKEMAERIGAK
VNTRGYNAFTVLVQLFCKTKILFEVNAKEFHPQPKVQSAVIHLENLHNSVNFNIEELGKFLRICFLNKRKKLKNNLSNIY
DIKIINQMFIDYNLDMNLRAENIEPKMFLKLFNYLNR

Sequences:

>Translated_277_residues
MNKSFIKNKLKQESFVPSKKMGQNFLLSNNIKNKIVDVANINKDDLILEIGPG*GAITEILVQKTNILIAIELDKRLYAH
LKTYIKTSNFHIINNDVLCVDLDNLILDYNNTQKIQKIKVVANLPYAISSKIVLKIIQSKLINDAYIMVQKEMAERIGAK
VNTRGYNAFTVLVQLFCKTKILFEVNAKEFHPQPKVQSAVIHLENLHNSVNFNIEELGKFLRICFLNKRKKLKNNLSNIY
DIKIINQMFIDYNLDMNLRAENIEPKMFLKLFNYLNR
>Mature_277_residues
MNKSFIKNKLKQESFVPSKKMGQNFLLSNNIKNKIVDVANINKDDLILEIGPG*GAITEILVQKTNILIAIELDKRLYAH
LKTYIKTSNFHIINNDVLCVDLDNLILDYNNTQKIQKIKVVANLPYAISSKIVLKIIQSKLINDAYIMVQKEMAERIGAK
VNTRGYNAFTVLVQLFCKTKILFEVNAKEFHPQPKVQSAVIHLENLHNSVNFNIEELGKFLRICFLNKRKKLKNNLSNIY
DIKIINQMFIDYNLDMNLRAENIEPKMFLKLFNYLNR

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=217, Percent_Identity=33.6405529953917, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI156415992, Length=204, Percent_Identity=25.9803921568627, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1786236, Length=263, Percent_Identity=31.1787072243346, Blast_Score=130, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI25146882, Length=228, Percent_Identity=32.0175438596491, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25141369, Length=255, Percent_Identity=23.5294117647059, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324989, Length=227, Percent_Identity=28.1938325991189, Blast_Score=92, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21358017, Length=267, Percent_Identity=29.5880149812734, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21357273, Length=201, Percent_Identity=29.8507462686567, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 31941; Mature: 31941

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSFIKNKLKQESFVPSKKMGQNFLLSNNIKNKIVDVANINKDDLILEIGPGGAITEIL
CCHHHHHHHHHHHHCCCCHHHCCCEEECCCCHHHEEEHCCCCCCCEEEEECCCCHHHHHH
VQKTNILIAIELDKRLYAHLKTYIKTSNFHIINNDVLCVDLDNLILDYNNTQKIQKIKVV
HHHCCEEEEEEECHHHHHHHHHHHCCCCEEEECCCEEEEEHHHEEEECCCCHHHHEEEEE
ANLPYAISSKIVLKIIQSKLINDAYIMVQKEMAERIGAKVNTRGYNAFTVLVQLFCKTKI
EECCHHHHHHHHHHHHHHHHCCHHHEEEEHHHHHHHCCEECCCCCHHHHHHHHHHHHHEE
LFEVNAKEFHPQPKVQSAVIHLENLHNSVNFNIEELGKFLRICFLNKRKKLKNNLSNIYD
EEEECHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEH
IKIINQMFIDYNLDMNLRAENIEPKMFLKLFNYLNR
HEEEEEEHEEECCCCEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNKSFIKNKLKQESFVPSKKMGQNFLLSNNIKNKIVDVANINKDDLILEIGPGGAITEIL
CCHHHHHHHHHHHHCCCCHHHCCCEEECCCCHHHEEEHCCCCCCCEEEEECCCCHHHHHH
VQKTNILIAIELDKRLYAHLKTYIKTSNFHIINNDVLCVDLDNLILDYNNTQKIQKIKVV
HHHCCEEEEEEECHHHHHHHHHHHCCCCEEEECCCEEEEEHHHEEEECCCCHHHHEEEEE
ANLPYAISSKIVLKIIQSKLINDAYIMVQKEMAERIGAKVNTRGYNAFTVLVQLFCKTKI
EECCHHHHHHHHHHHHHHHHCCHHHEEEEHHHHHHHCCEECCCCCHHHHHHHHHHHHHEE
LFEVNAKEFHPQPKVQSAVIHLENLHNSVNFNIEELGKFLRICFLNKRKKLKNNLSNIYD
EEEECHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEH
IKIINQMFIDYNLDMNLRAENIEPKMFLKLFNYLNR
HEEEEEEHEEECCCCEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA