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The map label for this gene is nfo [H]

Identifier: 170762414

GI number: 170762414

Start: 350915

End: 351835

Strand: Direct

Name: nfo [H]

Synonym: UPA3_0321

Alternate gene names: 170762414

Gene position: 350915-351835 (Clockwise)

Preceding gene: 170762270

Following gene: 170761894

Centisome position: 46.68

GC content: 26.82

Gene sequence:

>921_bases
ATGAATAATATGGATAAATATAATTTAATTATAGGTAGTCATGTAAGTTTAAAAGCTAAAGATTTCTTTTATGGTAGTGT
AAAAGAAGCATTATCTTATGGTTCTAACACGTTTATGGTTTATACTGGAGCTCCACAAAATACAAAACGTCAACCGATTA
AATCATTTAAAATTGAAGAAGCACATAATTTGTTAAAAAAACATAATATTAATTTGGATGATTTAATTGTTCACGCTCCA
TATATTATCAACCCTTGTTCTTCTAAAAAGAATGTTCGTGAACTTGCTAAAGAATTTTTAATTCAGGAAATCCAACGAAC
AGAATCTATGGGGATCACTAAACTTGTTTTACATCCAGGATCACGTTTAGAACAAAATGAAGATATAGCTTTAGAACAAG
TTTATACAATGTTAAATGATATTTTTTCAACTATTAATACTAATGTAATTGTTTGCTTAGAGACCATGGCTGGTAAAGGT
TCTGAAATTGGGGTAAATATAAAACAATTAAAAACCATTATAGACAATGTTCATTCTAAAAAAAATATTGGCGTTTGTTT
AGATACTTGCCATATGAATGATAGCGGTTTGATTTTAGATTATTATAATTTTAATCAATATTTAAAAGAATTTGATGCAC
AAATTGGTATTAATTATATTAAAGTATTACATATTAACGATTCTAAAAACCCTTGTGGTGCAAACAAAGACCGCCACGAA
AATTTAGGATATGGAACGATAGGTTTTGCAAATTTAATTAATATTATTTACCATCCCTTATTAAATAACATCCCTAAAAT
TTTAGAAACGCCTTGGTTTAATGTTAATGATGAATTAATTCCTTTATATAAGCATGAAATAAAAATGATTCGCGACTGCA
AGTGGTATGACATTAAAAACAAATTATTATCGAAGAAATAA

Upstream 100 bases:

>100_bases
CATCAATATACTGAAGAAGTTTTAAATTGATATAAACCAATTAAGTTTAAAAAATAATTGTAAGTAAATTAATTAAATTT
CTATTTTAAGGATGATGAAA

Downstream 100 bases:

>100_bases
AAACTATTTTTAAATACAAATAAGTATTTCTAAAAAAAAAAAAAAAAAGGAGAAGTTCTCCTTTTTTTCGATCACAATTT
TTTATGATCTTATTGGTTAT

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MNNMDKYNLIIGSHVSLKAKDFFYGSVKEALSYGSNTFMVYTGAPQNTKRQPIKSFKIEEAHNLLKKHNINLDDLIVHAP
YIINPCSSKKNVRELAKEFLIQEIQRTESMGITKLVLHPGSRLEQNEDIALEQVYTMLNDIFSTINTNVIVCLETMAGKG
SEIGVNIKQLKTIIDNVHSKKNIGVCLDTCHMNDSGLILDYYNFNQYLKEFDAQIGINYIKVLHINDSKNPCGANKDRHE
NLGYGTIGFANLINIIYHPLLNNIPKILETPWFNVNDELIPLYKHEIKMIRDCKWYDIKNKLLSKK

Sequences:

>Translated_306_residues
MNNMDKYNLIIGSHVSLKAKDFFYGSVKEALSYGSNTFMVYTGAPQNTKRQPIKSFKIEEAHNLLKKHNINLDDLIVHAP
YIINPCSSKKNVRELAKEFLIQEIQRTESMGITKLVLHPGSRLEQNEDIALEQVYTMLNDIFSTINTNVIVCLETMAGKG
SEIGVNIKQLKTIIDNVHSKKNIGVCLDTCHMNDSGLILDYYNFNQYLKEFDAQIGINYIKVLHINDSKNPCGANKDRHE
NLGYGTIGFANLINIIYHPLLNNIPKILETPWFNVNDELIPLYKHEIKMIRDCKWYDIKNKLLSKK
>Mature_306_residues
MNNMDKYNLIIGSHVSLKAKDFFYGSVKEALSYGSNTFMVYTGAPQNTKRQPIKSFKIEEAHNLLKKHNINLDDLIVHAP
YIINPCSSKKNVRELAKEFLIQEIQRTESMGITKLVLHPGSRLEQNEDIALEQVYTMLNDIFSTINTNVIVCLETMAGKG
SEIGVNIKQLKTIIDNVHSKKNIGVCLDTCHMNDSGLILDYYNFNQYLKEFDAQIGINYIKVLHINDSKNPCGANKDRHE
NLGYGTIGFANLINIIYHPLLNNIPKILETPWFNVNDELIPLYKHEIKMIRDCKWYDIKNKLLSKK

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=262, Percent_Identity=30.5343511450382, Blast_Score=130, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17531193, Length=275, Percent_Identity=32.7272727272727, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6322735, Length=293, Percent_Identity=33.4470989761092, Blast_Score=150, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 35064; Mature: 35064

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNMDKYNLIIGSHVSLKAKDFFYGSVKEALSYGSNTFMVYTGAPQNTKRQPIKSFKIEE
CCCCCCEEEEEECCEEEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCHHH
AHNLLKKHNINLDDLIVHAPYIINPCSSKKNVRELAKEFLIQEIQRTESMGITKLVLHPG
HHHHHHHCCCCHHHHEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
SRLEQNEDIALEQVYTMLNDIFSTINTNVIVCLETMAGKGSEIGVNIKQLKTIIDNVHSK
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
KNIGVCLDTCHMNDSGLILDYYNFNQYLKEFDAQIGINYIKVLHINDSKNPCGANKDRHE
CCCCEEEEEEEECCCCEEEEEHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCC
NLGYGTIGFANLINIIYHPLLNNIPKILETPWFNVNDELIPLYKHEIKMIRDCKWYDIKN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHCCCHHHHHH
KLLSKK
HHHCCC
>Mature Secondary Structure
MNNMDKYNLIIGSHVSLKAKDFFYGSVKEALSYGSNTFMVYTGAPQNTKRQPIKSFKIEE
CCCCCCEEEEEECCEEEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCHHH
AHNLLKKHNINLDDLIVHAPYIINPCSSKKNVRELAKEFLIQEIQRTESMGITKLVLHPG
HHHHHHHCCCCHHHHEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
SRLEQNEDIALEQVYTMLNDIFSTINTNVIVCLETMAGKGSEIGVNIKQLKTIIDNVHSK
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
KNIGVCLDTCHMNDSGLILDYYNFNQYLKEFDAQIGINYIKVLHINDSKNPCGANKDRHE
CCCCEEEEEEEECCCCEEEEEHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCC
NLGYGTIGFANLINIIYHPLLNNIPKILETPWFNVNDELIPLYKHEIKMIRDCKWYDIKN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHCCCHHHHHH
KLLSKK
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11048724 [H]