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The map label for this gene is topA

Identifier: 170762408

GI number: 170762408

Start: 738141

End: 740186

Strand: Reverse

Name: topA

Synonym: UPA3_0630

Alternate gene names: 170762408

Gene position: 740186-738141 (Counterclockwise)

Preceding gene: 170762318

Following gene: 170762328

Centisome position: 98.47

GC content: 27.37

Gene sequence:

>2046_bases
ATGGGAGAAAAAATGCAAAAAGATTTAATAGTAGTAGAGTCACCAAATAAAATTAAATCAATTCAGTCTTATTTAGGTGA
TAGTTATAATGTAATAGCTACATGTGGACATTTACGTGAGCTCGCAAAAAAATCTGGTTATGATCCAAAAACTTATGATT
TAAAATGAGAAATAATAAAAACAACAAATAAAAAATTATCGAAAAAACAACAAATAAAAACAATTATTGATTTAGCAAAA
AAATCAAATAATATCTATTTAGCAACTGACCCTGATCGTGAAGGTGAAGCTATTAGTTGACATGTTTATGATTTATTACC
AAAAAAAGATAAAGAACGTGTTAAAAGAATAACTTTTAATGAAATAACACAGAAAGCTATTTTAAAATCTATTGATGAAA
AACATGATATTGATTTTAATTTGGTAGATTCGCAATTTGCAAGACGAATTTTAGATCGAGTTATTGGTTATAAACTTTCT
GGATTAGTTAAAAAAAACTTACATGGCTTATCTGCTGGGCGAGTACAATCGATCGCTTTATTATTTGTAGTTGAGAGAGA
GTTAGAAAGACGTTCTTTTGTTAAAGAAGAATGATATCAAATTGAAGGAATAATTGAAAAAAACATTTTTGTATCTTTAA
GAAAAATTCCCTATGATGTTGAATTATATAATCAGAAAGATAATAGCGATAGTGATTTAAAATTTCTACACAAAAAAGAT
GCACAGCGTGTGATTGATGATTTAAGTGATATTTTTAAAGTTTATAGTATTGATGATTTGAAAATCACCAAAGGCGATGT
TCAATTACCATTAACAACTGATAAATTATTACAAATCGCATCCAATAATTTTGGTTGAAGTGCTAATAAAACAACTTTAA
TAGCTCAACAAATGTTTGAAGGATTAGAAATTAATAATCAGCAATTAGCTTTAATAACTTATCCAAGAACAGATAGCGAG
CGATTAAGTGATGATTTTTTACAAGAAGCTAATGCCTTTATTGAAAATAATTTTGGTAAAGAATATCTAGAAAATTTTAA
AAATAAAAAAATTAATAGAAAAGAAGTAAATATTCAAGACGCACATGAAGCAATTCGCCCTGTTGATATTAATTTAAAAC
CTGAAGAAATTAAAGATTATGTTTCATTAGATATTTATAAGCTGTATAACTTAGTTTGAAATAAAACACTATCAGCTTTA
ATGAAAGTGCCAACTTTTAATAAGCAAGTAATTCGTTTTATTAATAATGAATATAAATTTTATACAGCTTATAAAACAAT
TCGAACTTTAGGATATTGAATTTTAGATTTTTATAAAAAGCAACGTGATGAATTAGAATTCCAACCTCCTATAATTAATG
TTGGTGATGAATTTAAAAAAGAAAAGATCGATTTAATACAAAAAGAAACTACGCCACCGCCTTATTACACAGAAGCCTCT
TTAATTGCAGCGCTAAAAAATGCCGGAGTAGGTCGGCCTTCAACATATGCAACAATGGCAACTATTGGCACCACTAGGGG
TTACATCAATAAAGATAAGAGTAAATTAATTCCAACAGAATTAGGGATTAAAGTCATCCAAGAGTTAAAAGAAGCCTTTC
CAAAAGTGATTTCTATTAAATTTACATCTGAGATGGAAGAACGTTTAGATGTTATAGCTAATGGCAAATTATTTTGAAAA
GATTTAGTTAGCACTTTTATTCCTATTTTTGAAGAAGAAGTAAAACAAGCATACACTAAAATCGAAAAAGTTCCTGATGA
AAAAATTAATCGATTATGTCCTATATGTGCTCATGATTTATTGATTAAAAGATCTAGAATGAACACTCGGTTCATTGCGT
GTTCAAATTTCCCTACATGTCGTTTTACAGAATCGTTAGAGAAACCAGAAATTCTTAACGAATTATGTCCTAACTGTTCA
AAATCATTGGTTAAACGTAAAAATCGTAAAAATAAATATTTTATTGGATGCACAGGTTATCCTGAATGTAATTTTATTCG
TAATTTAGATGAATCAAACAATGATAAAAACGATTTTAATCAATAA

Upstream 100 bases:

>100_bases
TTTATCAATTTATTAAAAATTCAAAATTAATAAATTAAACAACAACTATAAAATATTATAATTATTTACAATTGTTAAAA
CGAGCAAATAATTGTAGTAA

Downstream 100 bases:

>100_bases
TAATATAATATTAAAAAGAGGAAAATTTATGTTAATTAAATTAGAAAGTAACCAAAATTTAAATGAATTATTAAAGGAAA
ATCATTCTAAACCAATATTA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 681; Mature: 680

Protein sequence:

>681_residues
MGEKMQKDLIVVESPNKIKSIQSYLGDSYNVIATCGHLRELAKKSGYDPKTYDLKWEIIKTTNKKLSKKQQIKTIIDLAK
KSNNIYLATDPDREGEAISWHVYDLLPKKDKERVKRITFNEITQKAILKSIDEKHDIDFNLVDSQFARRILDRVIGYKLS
GLVKKNLHGLSAGRVQSIALLFVVERELERRSFVKEEWYQIEGIIEKNIFVSLRKIPYDVELYNQKDNSDSDLKFLHKKD
AQRVIDDLSDIFKVYSIDDLKITKGDVQLPLTTDKLLQIASNNFGWSANKTTLIAQQMFEGLEINNQQLALITYPRTDSE
RLSDDFLQEANAFIENNFGKEYLENFKNKKINRKEVNIQDAHEAIRPVDINLKPEEIKDYVSLDIYKLYNLVWNKTLSAL
MKVPTFNKQVIRFINNEYKFYTAYKTIRTLGYWILDFYKKQRDELEFQPPIINVGDEFKKEKIDLIQKETTPPPYYTEAS
LIAALKNAGVGRPSTYATMATIGTTRGYINKDKSKLIPTELGIKVIQELKEAFPKVISIKFTSEMEERLDVIANGKLFWK
DLVSTFIPIFEEEVKQAYTKIEKVPDEKINRLCPICAHDLLIKRSRMNTRFIACSNFPTCRFTESLEKPEILNELCPNCS
KSLVKRKNRKNKYFIGCTGYPECNFIRNLDESNNDKNDFNQ

Sequences:

>Translated_681_residues
MGEKMQKDLIVVESPNKIKSIQSYLGDSYNVIATCGHLRELAKKSGYDPKTYDLK*EIIKTTNKKLSKKQQIKTIIDLAK
KSNNIYLATDPDREGEAIS*HVYDLLPKKDKERVKRITFNEITQKAILKSIDEKHDIDFNLVDSQFARRILDRVIGYKLS
GLVKKNLHGLSAGRVQSIALLFVVERELERRSFVKEE*YQIEGIIEKNIFVSLRKIPYDVELYNQKDNSDSDLKFLHKKD
AQRVIDDLSDIFKVYSIDDLKITKGDVQLPLTTDKLLQIASNNFG*SANKTTLIAQQMFEGLEINNQQLALITYPRTDSE
RLSDDFLQEANAFIENNFGKEYLENFKNKKINRKEVNIQDAHEAIRPVDINLKPEEIKDYVSLDIYKLYNLV*NKTLSAL
MKVPTFNKQVIRFINNEYKFYTAYKTIRTLGY*ILDFYKKQRDELEFQPPIINVGDEFKKEKIDLIQKETTPPPYYTEAS
LIAALKNAGVGRPSTYATMATIGTTRGYINKDKSKLIPTELGIKVIQELKEAFPKVISIKFTSEMEERLDVIANGKLF*K
DLVSTFIPIFEEEVKQAYTKIEKVPDEKINRLCPICAHDLLIKRSRMNTRFIACSNFPTCRFTESLEKPEILNELCPNCS
KSLVKRKNRKNKYFIGCTGYPECNFIRNLDESNNDKNDFNQ
>Mature_680_residues
GEKMQKDLIVVESPNKIKSIQSYLGDSYNVIATCGHLRELAKKSGYDPKTYDLK*EIIKTTNKKLSKKQQIKTIIDLAKK
SNNIYLATDPDREGEAIS*HVYDLLPKKDKERVKRITFNEITQKAILKSIDEKHDIDFNLVDSQFARRILDRVIGYKLSG
LVKKNLHGLSAGRVQSIALLFVVERELERRSFVKEE*YQIEGIIEKNIFVSLRKIPYDVELYNQKDNSDSDLKFLHKKDA
QRVIDDLSDIFKVYSIDDLKITKGDVQLPLTTDKLLQIASNNFG*SANKTTLIAQQMFEGLEINNQQLALITYPRTDSER
LSDDFLQEANAFIENNFGKEYLENFKNKKINRKEVNIQDAHEAIRPVDINLKPEEIKDYVSLDIYKLYNLV*NKTLSALM
KVPTFNKQVIRFINNEYKFYTAYKTIRTLGY*ILDFYKKQRDELEFQPPIINVGDEFKKEKIDLIQKETTPPPYYTEASL
IAALKNAGVGRPSTYATMATIGTTRGYINKDKSKLIPTELGIKVIQELKEAFPKVISIKFTSEMEERLDVIANGKLF*KD
LVSTFIPIFEEEVKQAYTKIEKVPDEKINRLCPICAHDLLIKRSRMNTRFIACSNFPTCRFTESLEKPEILNELCPNCSK
SLVKRKNRKNKYFIGCTGYPECNFIRNLDESNNDKNDFNQ

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=587, Percent_Identity=24.3611584327087, Blast_Score=102, Evalue=2e-21,
Organism=Homo sapiens, GI10835218, Length=623, Percent_Identity=21.9903691813804, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1787529, Length=694, Percent_Identity=30.835734870317, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1788061, Length=632, Percent_Identity=24.6835443037975, Blast_Score=119, Evalue=8e-28,
Organism=Escherichia coli, GI48994931, Length=80, Percent_Identity=38.75, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32563869, Length=584, Percent_Identity=22.6027397260274, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17555378, Length=483, Percent_Identity=23.8095238095238, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013826
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 77775; Mature: 77644

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEKMQKDLIVVESPNKIKSIQSYLGDSYNVIATCGHLRELAKKSGYDPKTYDLKEIIKT
CCCCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
TNKKLSKKQQIKTIIDLAKKSNNIYLATDPDREGEAISHVYDLLPKKDKERVKRITFNEI
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
TQKAILKSIDEKHDIDFNLVDSQFARRILDRVIGYKLSGLVKKNLHGLSAGRVQSIALLF
HHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VVERELERRSFVKEEYQIEGIIEKNIFVSLRKIPYDVELYNQKDNSDSDLKFLHKKDAQR
HHHHHHHHHHHHHHHHHHHHEEHHHHHEEHHHCCCEEEEECCCCCCCHHHHHHHHHHHHH
VIDDLSDIFKVYSIDDLKITKGDVQLPLTTDKLLQIASNNFGSANKTTLIAQQMFEGLEI
HHHHHHHHHHHHCCCCEEEECCCEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCC
NNQQLALITYPRTDSERLSDDFLQEANAFIENNFGKEYLENFKNKKINRKEVNIQDAHEA
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHCCHHHHHHC
IRPVDINLKPEEIKDYVSLDIYKLYNLVNKTLSALMKVPTFNKQVIRFINNEYKFYTAYK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEHHHHH
TIRTLGYILDFYKKQRDELEFQPPIINVGDEFKKEKIDLIQKETTPPPYYTEASLIAALK
HHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
NAGVGRPSTYATMATIGTTRGYINKDKSKLIPTELGIKVIQELKEAFPKVISIKFTSEME
HCCCCCCCHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHEEEEEHHHHH
ERLDVIANGKLFKDLVSTFIPIFEEEVKQAYTKIEKVPDEKINRLCPICAHDLLIKRSRM
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
NTRFIACSNFPTCRFTESLEKPEILNELCPNCSKSLVKRKNRKNKYFIGCTGYPECNFIR
CCEEEEECCCCCCCCHHHCCCHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHH
NLDESNNDKNDFNQ
CCCCCCCCCCCCCC
>Mature Secondary Structure 
GEKMQKDLIVVESPNKIKSIQSYLGDSYNVIATCGHLRELAKKSGYDPKTYDLKEIIKT
CCCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
TNKKLSKKQQIKTIIDLAKKSNNIYLATDPDREGEAISHVYDLLPKKDKERVKRITFNEI
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
TQKAILKSIDEKHDIDFNLVDSQFARRILDRVIGYKLSGLVKKNLHGLSAGRVQSIALLF
HHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VVERELERRSFVKEEYQIEGIIEKNIFVSLRKIPYDVELYNQKDNSDSDLKFLHKKDAQR
HHHHHHHHHHHHHHHHHHHHEEHHHHHEEHHHCCCEEEEECCCCCCCHHHHHHHHHHHHH
VIDDLSDIFKVYSIDDLKITKGDVQLPLTTDKLLQIASNNFGSANKTTLIAQQMFEGLEI
HHHHHHHHHHHHCCCCEEEECCCEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCC
NNQQLALITYPRTDSERLSDDFLQEANAFIENNFGKEYLENFKNKKINRKEVNIQDAHEA
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHCCHHHHHHC
IRPVDINLKPEEIKDYVSLDIYKLYNLVNKTLSALMKVPTFNKQVIRFINNEYKFYTAYK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEHHHHH
TIRTLGYILDFYKKQRDELEFQPPIINVGDEFKKEKIDLIQKETTPPPYYTEASLIAALK
HHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
NAGVGRPSTYATMATIGTTRGYINKDKSKLIPTELGIKVIQELKEAFPKVISIKFTSEME
HCCCCCCCHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHEEEEEHHHHH
ERLDVIANGKLFKDLVSTFIPIFEEEVKQAYTKIEKVPDEKINRLCPICAHDLLIKRSRM
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
NTRFIACSNFPTCRFTESLEKPEILNELCPNCSKSLVKRKNRKNKYFIGCTGYPECNFIR
CCEEEEECCCCCCCCHHHCCCHHHHHHHCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHH
NLDESNNDKNDFNQ
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]