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The map label for this gene is pyrG

Identifier: 170762342

GI number: 170762342

Start: 320086

End: 321699

Strand: Direct

Name: pyrG

Synonym: UPA3_0294

Alternate gene names: 170762342

Gene position: 320086-321699 (Clockwise)

Preceding gene: 170762274

Following gene: 170762008

Centisome position: 42.58

GC content: 25.77

Gene sequence:

>1614_bases
ATGAAACATATACAAAAAACAAAATTTATTTTTGTAACTGGTGGTGTTTATTCATCATTAGGTAAAGGGGTTAGTGCTAG
TAGTATTGGTCGAATTCTTGTTGAATTAGGATATAGTGTTGCAATGCAAAAGCTTGATCCATATTTAAATATTGATCCCA
CTTATTTATCACCATTACAACATGGAGAAGTATTTGTTACAAAAGACGGTAAAGAAGCAGATTTAGATTTAGGTACTTAT
GAACGTTTTATTAATGCTGATTTAAATAAATATGCGTCAGTAACTTCAGGAAAAATTTATTATGAAATTTTAACAAAAGA
GCGTGAAAATGGTTTTGACGGTAAAACTGTTCAAACTATTCCACATGTCACATCTGCAGTTATTGATTACATTAAAAAAA
TTAAAGATAGTTTAAAAACTGATTTTATTATTGTTGAAATTGGAGGAACAATTGGAGATATTGAATCGCTTCCATTTATT
GAAGCTATTAGTCAATTTAAAACAATTTATGGTGTAAATAATGTAATGTTTATTCACTGTTCACCGCTTATTTATATTGA
AAAAGTAGGTGAATTAAAAACAAAACCAACTCAACATAGCGTTAAGACGTTACGTTCATTAGGAATTAATTTAGATTTAT
TGTTATTAAGAACTAATCAAAAATTAGATGAAGTAACGATTAAAAAATTAGCATGATCTTGTGGATTAGATATTGATATG
ATTTTTGCTGCTTATGATGTTGAGTCAGTTTATTTATTACCAAATGTTTTGTTTGAGCAAGGGATTCATAAAACTATTTT
AGATTTTTTTAGTCTACCTTTAAAAAATGATAATATTAATTCATGAATAGATTTTACTGATAAAATTACAACCTTTAAAA
AACATAATTTAGTTATAGGTTTAGTTGGAAAATATGTTGAATTACCCGATGCTTATAAATCAGTATTAGCGAGTTTAGAA
TTAGCAGCAATTGAATTAAATATTGATTTAAAGATTAAATATATTCAACCCCAAAATTTAAATGAAAACAACATAAATGA
AGAACTAAAAAAAATAAATGGTATTGTTATTCCAAGTATTGCAGGTTCAATAAAAGGCTGACCAGGAGCTTTATTAGCTG
CATCTTATGCAAGAAAAAACAATATTCCATTTTTAGCTGTGGGAACTGGCGTTAATATTGGTATTGGTGAATTCATTAAT
AATGTACTAAAACTTCCAATTGAATTTATTAATTTAGGCAATGGTGATTTTAGTTTTTTAAAAGACGCTTTCGTTAAAAA
TGAAATTGAAAATTATCGAATTGGAGAGTATTGTTCTAATATTCAAGCTAACACTATAACAAGCCAAATTTATCTCAAAC
AAAATCAATTGAACGAGCGCCATCGTCATCATTTTGAATTTAACAATCATTATATTAATAATTATTTTTTAAATCAAAAT
TGAAAAATTGGAGCAATTTCAGTGGATAATAATTATATTGATGTTTTAGAATACACAAAAAATCATTTTTATGTTTTAAC
AATTTTTAATCCAGAATATACATCAAAACCTTCAAAGGCAAATCCTTATTTTATTAATTTATTAAAGATGAGTTTAAAAA
TAAAAGAAAGTTAG

Upstream 100 bases:

>100_bases
ATACTTTTTTAAAAATTTATGTAAAAAACTTAGATCACTTACAAAAGATTATTTTAGAACTTCAAGCTAAAAATGTTATT
AATGAATGGAGATTGATCTA

Downstream 100 bases:

>100_bases
AATATGATTAATTACAGTAAACCACGAGGTACTATAGATTTATATAATAAAGAAATGAATGATTTTAAATTACTTGAAAA
TTTTTTATTATCAACTACTA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MKHIQKTKFIFVTGGVYSSLGKGVSASSIGRILVELGYSVAMQKLDPYLNIDPTYLSPLQHGEVFVTKDGKEADLDLGTY
ERFINADLNKYASVTSGKIYYEILTKERENGFDGKTVQTIPHVTSAVIDYIKKIKDSLKTDFIIVEIGGTIGDIESLPFI
EAISQFKTIYGVNNVMFIHCSPLIYIEKVGELKTKPTQHSVKTLRSLGINLDLLLLRTNQKLDEVTIKKLAWSCGLDIDM
IFAAYDVESVYLLPNVLFEQGIHKTILDFFSLPLKNDNINSWIDFTDKITTFKKHNLVIGLVGKYVELPDAYKSVLASLE
LAAIELNIDLKIKYIQPQNLNENNINEELKKINGIVIPSIAGSIKGWPGALLAASYARKNNIPFLAVGTGVNIGIGEFIN
NVLKLPIEFINLGNGDFSFLKDAFVKNEIENYRIGEYCSNIQANTITSQIYLKQNQLNERHRHHFEFNNHYINNYFLNQN
WKIGAISVDNNYIDVLEYTKNHFYVLTIFNPEYTSKPSKANPYFINLLKMSLKIKES

Sequences:

>Translated_537_residues
MKHIQKTKFIFVTGGVYSSLGKGVSASSIGRILVELGYSVAMQKLDPYLNIDPTYLSPLQHGEVFVTKDGKEADLDLGTY
ERFINADLNKYASVTSGKIYYEILTKERENGFDGKTVQTIPHVTSAVIDYIKKIKDSLKTDFIIVEIGGTIGDIESLPFI
EAISQFKTIYGVNNVMFIHCSPLIYIEKVGELKTKPTQHSVKTLRSLGINLDLLLLRTNQKLDEVTIKKLA*SCGLDIDM
IFAAYDVESVYLLPNVLFEQGIHKTILDFFSLPLKNDNINS*IDFTDKITTFKKHNLVIGLVGKYVELPDAYKSVLASLE
LAAIELNIDLKIKYIQPQNLNENNINEELKKINGIVIPSIAGSIKG*PGALLAASYARKNNIPFLAVGTGVNIGIGEFIN
NVLKLPIEFINLGNGDFSFLKDAFVKNEIENYRIGEYCSNIQANTITSQIYLKQNQLNERHRHHFEFNNHYINNYFLNQN
*KIGAISVDNNYIDVLEYTKNHFYVLTIFNPEYTSKPSKANPYFINLLKMSLKIKES
>Mature_537_residues
MKHIQKTKFIFVTGGVYSSLGKGVSASSIGRILVELGYSVAMQKLDPYLNIDPTYLSPLQHGEVFVTKDGKEADLDLGTY
ERFINADLNKYASVTSGKIYYEILTKERENGFDGKTVQTIPHVTSAVIDYIKKIKDSLKTDFIIVEIGGTIGDIESLPFI
EAISQFKTIYGVNNVMFIHCSPLIYIEKVGELKTKPTQHSVKTLRSLGINLDLLLLRTNQKLDEVTIKKLA*SCGLDIDM
IFAAYDVESVYLLPNVLFEQGIHKTILDFFSLPLKNDNINS*IDFTDKITTFKKHNLVIGLVGKYVELPDAYKSVLASLE
LAAIELNIDLKIKYIQPQNLNENNINEELKKINGIVIPSIAGSIKG*PGALLAASYARKNNIPFLAVGTGVNIGIGEFIN
NVLKLPIEFINLGNGDFSFLKDAFVKNEIENYRIGEYCSNIQANTITSQIYLKQNQLNERHRHHFEFNNHYINNYFLNQN
*KIGAISVDNNYIDVLEYTKNHFYVLTIFNPEYTSKPSKANPYFINLLKMSLKIKES

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=552, Percent_Identity=34.2391304347826, Blast_Score=318, Evalue=7e-87,
Organism=Homo sapiens, GI28559085, Length=551, Percent_Identity=36.1161524500907, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI28559083, Length=551, Percent_Identity=36.1161524500907, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI221316689, Length=551, Percent_Identity=36.1161524500907, Blast_Score=317, Evalue=2e-86,
Organism=Escherichia coli, GI1789142, Length=542, Percent_Identity=36.1623616236162, Blast_Score=336, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI25148299, Length=526, Percent_Identity=33.8403041825095, Blast_Score=292, Evalue=4e-79,
Organism=Saccharomyces cerevisiae, GI6319432, Length=573, Percent_Identity=35.9511343804538, Blast_Score=321, Evalue=1e-88,
Organism=Saccharomyces cerevisiae, GI6322563, Length=569, Percent_Identity=34.9736379613357, Blast_Score=313, Evalue=4e-86,
Organism=Drosophila melanogaster, GI24664469, Length=551, Percent_Identity=32.8493647912886, Blast_Score=306, Evalue=3e-83,
Organism=Drosophila melanogaster, GI21357815, Length=496, Percent_Identity=30.4435483870968, Blast_Score=248, Evalue=6e-66,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59929; Mature: 59929

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHIQKTKFIFVTGGVYSSLGKGVSASSIGRILVELGYSVAMQKLDPYLNIDPTYLSPLQ
CCCCCCEEEEEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCC
HGEVFVTKDGKEADLDLGTYERFINADLNKYASVTSGKIYYEILTKERENGFDGKTVQTI
CCCEEEECCCCCCCCCHHHHHHHHHCCHHHHHCCCCCEEEEEEEECCCCCCCCCCEEHHH
PHVTSAVIDYIKKIKDSLKTDFIIVEIGGTIGDIESLPFIEAISQFKTIYGVNNVMFIHC
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
SPLIYIEKVGELKTKPTQHSVKTLRSLGINLDLLLLRTNQKLDEVTIKKLASCGLDIDMI
CCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCEEE
FAAYDVESVYLLPNVLFEQGIHKTILDFFSLPLKNDNINSIDFTDKITTFKKHNLVIGLV
EEEECCCEEEEHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHEEECCCEEEEEC
GKYVELPDAYKSVLASLELAAIELNIDLKIKYIQPQNLNENNINEELKKINGIVIPSIAG
CCHHCCCHHHHHHHHHCEEEEEEEEEEEEEEEECCCCCCCCCHHHHHHHHCCEEEECCCC
SIKGPGALLAASYARKNNIPFLAVGTGVNIGIGEFINNVLKLPIEFINLGNGDFSFLKDA
CCCCCCHHHEEHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCCHHEEECCCCCHHHHHHH
FVKNEIENYRIGEYCSNIQANTITSQIYLKQNQLNERHRHHFEFNNHYINNYFLNQNKIG
HHHHHHCCCCHHHHHCCCCCCEEEEEEEEEHHHHHHHHHHCCCCCCEEEEEEEECCCCEE
AISVDNNYIDVLEYTKNHFYVLTIFNPEYTSKPSKANPYFINLLKMSLKIKES
EEEECCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEEEEECC
>Mature Secondary Structure
MKHIQKTKFIFVTGGVYSSLGKGVSASSIGRILVELGYSVAMQKLDPYLNIDPTYLSPLQ
CCCCCCEEEEEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCC
HGEVFVTKDGKEADLDLGTYERFINADLNKYASVTSGKIYYEILTKERENGFDGKTVQTI
CCCEEEECCCCCCCCCHHHHHHHHHCCHHHHHCCCCCEEEEEEEECCCCCCCCCCEEHHH
PHVTSAVIDYIKKIKDSLKTDFIIVEIGGTIGDIESLPFIEAISQFKTIYGVNNVMFIHC
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
SPLIYIEKVGELKTKPTQHSVKTLRSLGINLDLLLLRTNQKLDEVTIKKLASCGLDIDMI
CCEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCEEE
FAAYDVESVYLLPNVLFEQGIHKTILDFFSLPLKNDNINSIDFTDKITTFKKHNLVIGLV
EEEECCCEEEEHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHEEECCCEEEEEC
GKYVELPDAYKSVLASLELAAIELNIDLKIKYIQPQNLNENNINEELKKINGIVIPSIAG
CCHHCCCHHHHHHHHHCEEEEEEEEEEEEEEEECCCCCCCCCHHHHHHHHCCEEEECCCC
SIKGPGALLAASYARKNNIPFLAVGTGVNIGIGEFINNVLKLPIEFINLGNGDFSFLKDA
CCCCCCHHHEEHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCCHHEEECCCCCHHHHHHH
FVKNEIENYRIGEYCSNIQANTITSQIYLKQNQLNERHRHHFEFNNHYINNYFLNQNKIG
HHHHHHCCCCHHHHHCCCCCCEEEEEEEEEHHHHHHHHHHCCCCCCEEEEEEEECCCCEE
AISVDNNYIDVLEYTKNHFYVLTIFNPEYTSKPSKANPYFINLLKMSLKIKES
EEEECCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11048724 [H]