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The map label for this gene is ulaC [H]

Identifier: 170757757

GI number: 170757757

Start: 2297146

End: 2297583

Strand: Reverse

Name: ulaC [H]

Synonym: CLD_2483

Alternate gene names: 170757757

Gene position: 2297583-2297146 (Counterclockwise)

Preceding gene: 170754914

Following gene: 170756053

Centisome position: 58.05

GC content: 33.79

Gene sequence:

>438_bases
ATGCTTAAAAATTATATAAACGATCAGGTTGTAGAAGTAAACGTGGAAGTAACAAACTGGGAAGAGGCAGTAAGATTAGG
AGGAAAGCTATTAGAGGAAGATGGAGCCGTAGAACATAGCTATATTGATGCTATGGTAGATACGGTAAAAAACATGGGAC
CATACATTGTAATAGCACCTGGAATAGCAATGCCTCATGCAAGACCTGAAGCTGGAGCTAAAAATATAAGAATTGGACTT
TTGAAGCTTAAAAATCCAGTTAATTTTGGCAACGAAGAACATGATCCTGTAGATATTGTTATATTCTTATGTGCTGTAGA
TAATAAGGCACACATTGAGGTTTTAGGGGAACTTGTTCAGCTTATAGAAGATGATGATTTTTTAAAGATTATACGAAATG
CATCAACAAAGAAAGAAATACTAGATTATATAAAATAA

Upstream 100 bases:

>100_bases
ATCTAAAAGAATATTCTATTGAAACTGTAAGAGGATTTTACAAAGACGCATCTAGCCTTGGATTTTCAATTTTATAATTG
CAAGAAGGGCGGTAAAACTA

Downstream 100 bases:

>100_bases
AATATATAAACAAATTGGAGGGATTAATATGAATATTTTAACTGTATGTGGAAATGGAATTGGAAGCAGTCTTATGCTTG
CTATGAAGATAGAGGAAATA

Product: PTS system, L-ascorbate family, IIA component

Products: Protein Histidine; Sugar Phosphate. [C]

Alternate protein names: PTS system ascorbate-specific EIIA component [H]

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MLKNYINDQVVEVNVEVTNWEEAVRLGGKLLEEDGAVEHSYIDAMVDTVKNMGPYIVIAPGIAMPHARPEAGAKNIRIGL
LKLKNPVNFGNEEHDPVDIVIFLCAVDNKAHIEVLGELVQLIEDDDFLKIIRNASTKKEILDYIK

Sequences:

>Translated_145_residues
MLKNYINDQVVEVNVEVTNWEEAVRLGGKLLEEDGAVEHSYIDAMVDTVKNMGPYIVIAPGIAMPHARPEAGAKNIRIGL
LKLKNPVNFGNEEHDPVDIVIFLCAVDNKAHIEVLGELVQLIEDDDFLKIIRNASTKKEILDYIK
>Mature_145_residues
MLKNYINDQVVEVNVEVTNWEEAVRLGGKLLEEDGAVEHSYIDAMVDTVKNMGPYIVIAPGIAMPHARPEAGAKNIRIGL
LKLKNPVNFGNEEHDPVDIVIFLCAVDNKAHIEVLGELVQLIEDDDFLKIIRNASTKKEILDYIK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI2367359, Length=138, Percent_Identity=42.0289855072464, Blast_Score=117, Evalue=3e-28,
Organism=Escherichia coli, GI1790755, Length=141, Percent_Identity=37.5886524822695, Blast_Score=111, Evalue=2e-26,
Organism=Escherichia coli, GI1789302, Length=145, Percent_Identity=34.4827586206897, Blast_Score=82, Evalue=1e-17,
Organism=Escherichia coli, GI1790027, Length=126, Percent_Identity=31.7460317460317, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 16205; Mature: 16205

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKNYINDQVVEVNVEVTNWEEAVRLGGKLLEEDGAVEHSYIDAMVDTVKNMGPYIVIAP
CCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEC
GIAMPHARPEAGAKNIRIGLLKLKNPVNFGNEEHDPVDIVIFLCAVDNKAHIEVLGELVQ
CCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHH
LIEDDDFLKIIRNASTKKEILDYIK
HHCCCHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MLKNYINDQVVEVNVEVTNWEEAVRLGGKLLEEDGAVEHSYIDAMVDTVKNMGPYIVIAP
CCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEC
GIAMPHARPEAGAKNIRIGLLKLKNPVNFGNEEHDPVDIVIFLCAVDNKAHIEVLGELVQ
CCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHH
LIEDDDFLKIIRNASTKKEILDYIK
HHCCCHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein N-Phosphohistidine; Sugar [C]

Specific reaction: Protein N-Phosphohistidine + Sugar = Protein Histidine + Sugar Phosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]