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The map label for this gene is yclK [H]

Identifier: 170757730

GI number: 170757730

Start: 730513

End: 731934

Strand: Direct

Name: yclK [H]

Synonym: CLD_0148

Alternate gene names: 170757730

Gene position: 730513-731934 (Clockwise)

Preceding gene: 170757250

Following gene: 170756542

Centisome position: 18.46

GC content: 24.26

Gene sequence:

>1422_bases
ATGAAAATAAGATATAAATTTATAACTGGTTTAATATTTATACTGATAGTTTCTATGATAGTAATGAATGTGGCAATAAC
ACATGTTTTAAAGTCTAATATGGAAAATAGTATAAATAATTTTTTAAAACAAGTAATGAATAGCACTCACGAGTATGTAA
AGTACAACCTTGTAACTAATAGTTTTAAGGATAAAAAAGAATCATTGGTTGAAGAAGGGAATTACATAATAAAACATATA
TCTTTAAATTATGAATGTAAATGTGATATAAGAGATATAAATTATATATTGATAGAAGGAAATGTTCCAGAGGAATTCAG
AAGCATAACAAAAAAAAGTAAAAAAGTAGCAATGGATGGTAAAGCCGTAGTAGATTTGAAATATAGATATAATGGAGTAG
ATGCAATACTAACTTATCCTATATATATAGATAATGAATATGTGGGGATCATAAGCATAGTAAAGAATTATGACTCGAAA
TATAGGGATTATAAAAATACTATAAATATTATAAATATTATAGAACTTGGAACATTTATAGTTATATTTATATTTTTATT
TTTAAGAACAAATAAAATTACTAAACCAATTACGGAATTAACAGATGCAATAAAAAAACTAGGATATGGTGATTATAATA
TTTCCATTGCCGAACATGGAAAAGATGAGGTAGCAATATTAGCAAGAGAATTTATAAATATGAGAGATAAAATAAAAGAA
CAAATAGAAACTATTGAATCAGAAAAGAACAAAGTTTATAAATTAGAAAAAGGAAGAAAGGAATTTTTTAATAGCGTAAC
CCATGAATTAAAAACACCATTGACAGCTATATCAGGATATGCTGAGTTACTACTTACAGGAATGGTCAAGGATAAAGACT
TTGATAAAAGAGCTATTGAAAGAATATATTCAGAAAGTGACAGGCTTCATAAGTTAGTATTAGATCTTATAGAGGTATCT
AAAGGTATGTGTGTTATCAAAGAAGAACTTAATTATGTTGATATGAAAGAGCTTATTATTCAAAGTTGTAGTGATATGAA
TATAAAGGCTAATAAATATTCCTTAAAAATAATACAAAATATAAGTGAAGGAACAGTTAAGGTACAACAGGATAAAATTA
GACAAGTACTAATCAATATAATTGATAATGCAATAAAATATTCCTATGGTGGAAATGAGATATATGTTAATTCTTACATA
TCAGACAATAAATATATTATTGAAGTAATAAATAATAGTAATCCAATTCCAGATGAAATATTTAATAGTATATTTGAACC
TTTTATAAAATCTAATAATGATAATAAAGATAGTAGAGGACTTGGTTTATATTTATGTAATGAAATAGTCAAAGAGCATG
ACGGTGAAATAACTATAGAAAATGGCTCTCTAATAAAAGTAAAAATTAATTTAATAATTTAA

Upstream 100 bases:

>100_bases
GACGTTCATGTTAGAAGATTAAGAAGTAAGCTTGATAAAGAAAATGCCACATCTATCATAGACACTGCTTTTGGAATAGG
ATATGTAATGAGGTAGCTTT

Downstream 100 bases:

>100_bases
AAATATGAAAAGCGCACTAATCTTCATGATATAGGTGCTTTTTATTTTTATTATTAATTTTTATATGAATTTCAAAAAAC
TTAATTTTAATAATTAAGTT

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MKIRYKFITGLIFILIVSMIVMNVAITHVLKSNMENSINNFLKQVMNSTHEYVKYNLVTNSFKDKKESLVEEGNYIIKHI
SLNYECKCDIRDINYILIEGNVPEEFRSITKKSKKVAMDGKAVVDLKYRYNGVDAILTYPIYIDNEYVGIISIVKNYDSK
YRDYKNTINIINIIELGTFIVIFIFLFLRTNKITKPITELTDAIKKLGYGDYNISIAEHGKDEVAILAREFINMRDKIKE
QIETIESEKNKVYKLEKGRKEFFNSVTHELKTPLTAISGYAELLLTGMVKDKDFDKRAIERIYSESDRLHKLVLDLIEVS
KGMCVIKEELNYVDMKELIIQSCSDMNIKANKYSLKIIQNISEGTVKVQQDKIRQVLINIIDNAIKYSYGGNEIYVNSYI
SDNKYIIEVINNSNPIPDEIFNSIFEPFIKSNNDNKDSRGLGLYLCNEIVKEHDGEITIENGSLIKVKINLII

Sequences:

>Translated_473_residues
MKIRYKFITGLIFILIVSMIVMNVAITHVLKSNMENSINNFLKQVMNSTHEYVKYNLVTNSFKDKKESLVEEGNYIIKHI
SLNYECKCDIRDINYILIEGNVPEEFRSITKKSKKVAMDGKAVVDLKYRYNGVDAILTYPIYIDNEYVGIISIVKNYDSK
YRDYKNTINIINIIELGTFIVIFIFLFLRTNKITKPITELTDAIKKLGYGDYNISIAEHGKDEVAILAREFINMRDKIKE
QIETIESEKNKVYKLEKGRKEFFNSVTHELKTPLTAISGYAELLLTGMVKDKDFDKRAIERIYSESDRLHKLVLDLIEVS
KGMCVIKEELNYVDMKELIIQSCSDMNIKANKYSLKIIQNISEGTVKVQQDKIRQVLINIIDNAIKYSYGGNEIYVNSYI
SDNKYIIEVINNSNPIPDEIFNSIFEPFIKSNNDNKDSRGLGLYLCNEIVKEHDGEITIENGSLIKVKINLII
>Mature_473_residues
MKIRYKFITGLIFILIVSMIVMNVAITHVLKSNMENSINNFLKQVMNSTHEYVKYNLVTNSFKDKKESLVEEGNYIIKHI
SLNYECKCDIRDINYILIEGNVPEEFRSITKKSKKVAMDGKAVVDLKYRYNGVDAILTYPIYIDNEYVGIISIVKNYDSK
YRDYKNTINIINIIELGTFIVIFIFLFLRTNKITKPITELTDAIKKLGYGDYNISIAEHGKDEVAILAREFINMRDKIKE
QIETIESEKNKVYKLEKGRKEFFNSVTHELKTPLTAISGYAELLLTGMVKDKDFDKRAIERIYSESDRLHKLVLDLIEVS
KGMCVIKEELNYVDMKELIIQSCSDMNIKANKYSLKIIQNISEGTVKVQQDKIRQVLINIIDNAIKYSYGGNEIYVNSYI
SDNKYIIEVINNSNPIPDEIFNSIFEPFIKSNNDNKDSRGLGLYLCNEIVKEHDGEITIENGSLIKVKINLII

Specific function: Could be member of the two-component regulatory system yclK/yclJ. Potentially phosphorylates yclJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=286, Percent_Identity=24.1258741258741, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87082128, Length=300, Percent_Identity=24.3333333333333, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790861, Length=327, Percent_Identity=23.8532110091743, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788713, Length=249, Percent_Identity=25.7028112449799, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786912, Length=234, Percent_Identity=25.2136752136752, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786600, Length=307, Percent_Identity=22.1498371335505, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789808, Length=285, Percent_Identity=24.9122807017544, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790346, Length=283, Percent_Identity=20.1413427561837, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790436, Length=235, Percent_Identity=24.6808510638298, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693157, Length=265, Percent_Identity=26.4150943396226, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788549, Length=204, Percent_Identity=23.5294117647059, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54641; Mature: 54641

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRYKFITGLIFILIVSMIVMNVAITHVLKSNMENSINNFLKQVMNSTHEYVKYNLVTN
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEEECC
SFKDKKESLVEEGNYIIKHISLNYECKCDIRDINYILIEGNVPEEFRSITKKSKKVAMDG
CHHHHHHHHHHCCCEEEEEEEECEEEEEEEEECEEEEEECCCCHHHHHHHHHHHHEECCC
KAVVDLKYRYNGVDAILTYPIYIDNEYVGIISIVKNYDSKYRDYKNTINIINIIELGTFI
CEEEEEEEEECCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIFIFLFLRTNKITKPITELTDAIKKLGYGDYNISIAEHGKDEVAILAREFINMRDKIKE
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
QIETIESEKNKVYKLEKGRKEFFNSVTHELKTPLTAISGYAELLLTGMVKDKDFDKRAIE
HHHHHHHHCHHEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
RIYSESDRLHKLVLDLIEVSKGMCVIKEELNYVDMKELIIQSCSDMNIKANKYSLKIIQN
HHHCCHHHHHHHHHHHHHHCCCCEEEHHHCCCCCHHHHHHHCCCCCCEECCCEEEHHHHC
ISEGTVKVQQDKIRQVLINIIDNAIKYSYGGNEIYVNSYISDNKYIIEVINNSNPIPDEI
CCCCCEEEHHHHHHHHHHHHHHHHHEECCCCCEEEEEEEECCCCEEEEEECCCCCCHHHH
FNSIFEPFIKSNNDNKDSRGLGLYLCNEIVKEHDGEITIENGSLIKVKINLII
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEC
>Mature Secondary Structure
MKIRYKFITGLIFILIVSMIVMNVAITHVLKSNMENSINNFLKQVMNSTHEYVKYNLVTN
CEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEEEEECC
SFKDKKESLVEEGNYIIKHISLNYECKCDIRDINYILIEGNVPEEFRSITKKSKKVAMDG
CHHHHHHHHHHCCCEEEEEEEECEEEEEEEEECEEEEEECCCCHHHHHHHHHHHHEECCC
KAVVDLKYRYNGVDAILTYPIYIDNEYVGIISIVKNYDSKYRDYKNTINIINIIELGTFI
CEEEEEEEEECCEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIFIFLFLRTNKITKPITELTDAIKKLGYGDYNISIAEHGKDEVAILAREFINMRDKIKE
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
QIETIESEKNKVYKLEKGRKEFFNSVTHELKTPLTAISGYAELLLTGMVKDKDFDKRAIE
HHHHHHHHCHHEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
RIYSESDRLHKLVLDLIEVSKGMCVIKEELNYVDMKELIIQSCSDMNIKANKYSLKIIQN
HHHCCHHHHHHHHHHHHHHCCCCEEEHHHCCCCCHHHHHHHCCCCCCEECCCEEEHHHHC
ISEGTVKVQQDKIRQVLINIIDNAIKYSYGGNEIYVNSYISDNKYIIEVINNSNPIPDEI
CCCCCEEEHHHHHHHHHHHHHHHHHEECCCCCEEEEEEEECCCCEEEEEECCCCCCHHHH
FNSIFEPFIKSNNDNKDSRGLGLYLCNEIVKEHDGEITIENGSLIKVKINLII
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]