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The map label for this gene is dacF

Identifier: 170757721

GI number: 170757721

Start: 1982346

End: 1983569

Strand: Reverse

Name: dacF

Synonym: CLD_2781

Alternate gene names: 170757721

Gene position: 1983569-1982346 (Counterclockwise)

Preceding gene: 170756898

Following gene: 170756126

Centisome position: 50.11

GC content: 29.17

Gene sequence:

>1224_bases
ATGAAAAGAAAAATTTATTACTTTTTCAGTGTAATAATTACCATAACTTTTATTTTCGGTGTGTTTGTAACACCAGTAAG
CGCATATGACCAACAAAATCAGTTAAAAAAAGAAGAAAAAGCGGAAGATAAAGATTCTAATAAAGAAGGTGATTCAGATG
TAGATGCTAAATCAGCACTTCTTATTGAACCTACAACAGGAAAGGTTATTTATGAAAAAAATCCTCATGAAAAATTTGCT
CCAGCATCAGTTACAAAAATTATGACAATGCTTTTATCAATGGAAGCAGTAGATAGTGGCAAAATAAAGTTAACTGATAA
AGTTACTATAAGTGAAAATGCTAAAAAAATGGGTGGAAGTACCATGCTTTTAGATGTAGGAGAAGAAAGAACTGTTGAAG
ACCTTTTAAAAGGTATAGCAATAGCTTCTGGGAATGATGCTGCAGTAGCTATGGGAGAATACTTAGCAGGAAGCGAAGAA
GCTTTCGTAAAAATGATGAATGATAGAGCACAAAAATTAAATATGAAAGATACGTCTTTTAAAAATTGTACTGGGCTTAG
TGCAGAAGGTCACAAAACTTCAGCCTATGATATATCTATAATGTCAAGAGAACTATTAAAACATAAGCAAATATTAAAAT
ATACAGGAACATATATGGAAACCATATCAGAAGGAAGGAAAAGCCCTATAGGATTAGTAAATCATAATAAACTTGTTAGA
TTCTTTAAAGGCTGTGATGGGCTAAAAACAGGATTTACAGATGAAGCTAAATATTGTATATCAGCCACAGCAAAAAAAGA
TGGAGTAAGAATGTTAGCAGTAATAATGGGAGCTCCAACTTATAAAATAAGAAATAAAGATGCTAGTAAACTTATGAATT
ACGGATTTTCAAAATTTGAATGCAAAACTTTAGTTAAAAAAGGAGATGTAATATCAAAGGTACCTTTAAATTCTAAAGGA
GATAAATACTTTATGGCAAAATCCTGTGAAGAATTAGATGTTATAATAGAAAAAGGTAAGAAAACAAAAATAACTAATAA
ATGTATTATAGATGAAAATAAGAAAGAGTATAAAATAAACGAAGAAGTTGGTATATGTGAGTTTTATTCAGATGGTAATT
TAATAGGTAAAGTTAAGATAACTTCTGATAGAAATATAAGAAAATCAGGAATTTTTAATCACTTTAAAGGTGGATTTGAA
AGGTTAATAGATAAGGGTATATAA

Upstream 100 bases:

>100_bases
ATTATTCCTCCTAATAAAATTTAATTTTTCCCATAGAAAATTTTTCCGTGATAATATTATGAAATTCAGGAGAAAATATT
TGTAAAGGAGGGAACTATAA

Downstream 100 bases:

>100_bases
TAAGAATATATATGATTCAAAAGGAGGATAAAAGCTAACATGATTATTTCATGTTAGCTTTATTTAAGTATGAATATATT
AAATAAATTTACAGAAAAAG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MKRKIYYFFSVIITITFIFGVFVTPVSAYDQQNQLKKEEKAEDKDSNKEGDSDVDAKSALLIEPTTGKVIYEKNPHEKFA
PASVTKIMTMLLSMEAVDSGKIKLTDKVTISENAKKMGGSTMLLDVGEERTVEDLLKGIAIASGNDAAVAMGEYLAGSEE
AFVKMMNDRAQKLNMKDTSFKNCTGLSAEGHKTSAYDISIMSRELLKHKQILKYTGTYMETISEGRKSPIGLVNHNKLVR
FFKGCDGLKTGFTDEAKYCISATAKKDGVRMLAVIMGAPTYKIRNKDASKLMNYGFSKFECKTLVKKGDVISKVPLNSKG
DKYFMAKSCEELDVIIEKGKKTKITNKCIIDENKKEYKINEEVGICEFYSDGNLIGKVKITSDRNIRKSGIFNHFKGGFE
RLIDKGI

Sequences:

>Translated_407_residues
MKRKIYYFFSVIITITFIFGVFVTPVSAYDQQNQLKKEEKAEDKDSNKEGDSDVDAKSALLIEPTTGKVIYEKNPHEKFA
PASVTKIMTMLLSMEAVDSGKIKLTDKVTISENAKKMGGSTMLLDVGEERTVEDLLKGIAIASGNDAAVAMGEYLAGSEE
AFVKMMNDRAQKLNMKDTSFKNCTGLSAEGHKTSAYDISIMSRELLKHKQILKYTGTYMETISEGRKSPIGLVNHNKLVR
FFKGCDGLKTGFTDEAKYCISATAKKDGVRMLAVIMGAPTYKIRNKDASKLMNYGFSKFECKTLVKKGDVISKVPLNSKG
DKYFMAKSCEELDVIIEKGKKTKITNKCIIDENKKEYKINEEVGICEFYSDGNLIGKVKITSDRNIRKSGIFNHFKGGFE
RLIDKGI
>Mature_407_residues
MKRKIYYFFSVIITITFIFGVFVTPVSAYDQQNQLKKEEKAEDKDSNKEGDSDVDAKSALLIEPTTGKVIYEKNPHEKFA
PASVTKIMTMLLSMEAVDSGKIKLTDKVTISENAKKMGGSTMLLDVGEERTVEDLLKGIAIASGNDAAVAMGEYLAGSEE
AFVKMMNDRAQKLNMKDTSFKNCTGLSAEGHKTSAYDISIMSRELLKHKQILKYTGTYMETISEGRKSPIGLVNHNKLVR
FFKGCDGLKTGFTDEAKYCISATAKKDGVRMLAVIMGAPTYKIRNKDASKLMNYGFSKFECKTLVKKGDVISKVPLNSKG
DKYFMAKSCEELDVIIEKGKKTKITNKCIIDENKKEYKINEEVGICEFYSDGNLIGKVKITSDRNIRKSGIFNHFKGGFE
RLIDKGI

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=352, Percent_Identity=32.9545454545455, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI87082022, Length=361, Percent_Identity=29.9168975069252, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1786851, Length=355, Percent_Identity=30.7042253521127, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI87082054, Length=252, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 45360; Mature: 45360

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKIYYFFSVIITITFIFGVFVTPVSAYDQQNQLKKEEKAEDKDSNKEGDSDVDAKSAL
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
LIEPTTGKVIYEKNPHEKFAPASVTKIMTMLLSMEAVDSGKIKLTDKVTISENAKKMGGS
EEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHCCCC
TMLLDVGEERTVEDLLKGIAIASGNDAAVAMGEYLAGSEEAFVKMMNDRAQKLNMKDTSF
EEEEECCCCCCHHHHHHHHEEECCCCCEEEHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
KNCTGLSAEGHKTSAYDISIMSRELLKHKQILKYTGTYMETISEGRKSPIGLVNHNKLVR
CCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHH
FFKGCDGLKTGFTDEAKYCISATAKKDGVRMLAVIMGAPTYKIRNKDASKLMNYGFSKFE
HHHCCCCCCCCCCCCHHHHHCCCCCCCCCEEEEHHHCCCCEEECCCCHHHHHHCCCCHHH
CKTLVKKGDVISKVPLNSKGDKYFMAKSCEELDVIIEKGKKTKITNKCIIDENKKEYKIN
HHHHHHCCCCEEECCCCCCCCEEEECCCHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEC
EEVGICEFYSDGNLIGKVKITSDRNIRKSGIFNHFKGGFERLIDKGI
CCCCEEEEECCCCEEEEEEECCCCCCHHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRKIYYFFSVIITITFIFGVFVTPVSAYDQQNQLKKEEKAEDKDSNKEGDSDVDAKSAL
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEE
LIEPTTGKVIYEKNPHEKFAPASVTKIMTMLLSMEAVDSGKIKLTDKVTISENAKKMGGS
EEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHCCCC
TMLLDVGEERTVEDLLKGIAIASGNDAAVAMGEYLAGSEEAFVKMMNDRAQKLNMKDTSF
EEEEECCCCCCHHHHHHHHEEECCCCCEEEHHHHHCCCHHHHHHHHHHHHHHCCCCCCCC
KNCTGLSAEGHKTSAYDISIMSRELLKHKQILKYTGTYMETISEGRKSPIGLVNHNKLVR
CCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHH
FFKGCDGLKTGFTDEAKYCISATAKKDGVRMLAVIMGAPTYKIRNKDASKLMNYGFSKFE
HHHCCCCCCCCCCCCHHHHHCCCCCCCCCEEEEHHHCCCCEEECCCCHHHHHHCCCCHHH
CKTLVKKGDVISKVPLNSKGDKYFMAKSCEELDVIIEKGKKTKITNKCIIDENKKEYKIN
HHHHHHCCCCEEECCCCCCCCEEEECCCHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEC
EEVGICEFYSDGNLIGKVKITSDRNIRKSGIFNHFKGGFERLIDKGI
CCCCEEEEECCCCEEEEEEECCCCCCHHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]