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The map label for this gene is yfnG [H]

Identifier: 170757627

GI number: 170757627

Start: 2882463

End: 2883455

Strand: Reverse

Name: yfnG [H]

Synonym: CLD_1859

Alternate gene names: 170757627

Gene position: 2883455-2882463 (Counterclockwise)

Preceding gene: 170757325

Following gene: 170756450

Centisome position: 72.85

GC content: 35.55

Gene sequence:

>993_bases
ATGAACTTGAAGGGTAAAAAGGTGCTTGTTACAGGGGCAGAAGGCTTCATAGGAAGTCATCTAACCGAAAGACTTATAGA
ACTTGGAGCTGACGTGACAGCACTTGTTCAATACAATTCATTCAATAACTGGGGATGGATAGACACTTTTGACAAGAGGG
TTAAAGATAGTATTAATGTTGTGACTGGTGATGTAAGAGAATATGATGGAATGAAAAGAATTATAAAAGGGCAGGATGTA
GTTTTTCATCTGGCGGCTCTCATAGCTATTCCATATTCATATCTTTCACCTATGGCATATGTAAGAACAAATGTAGAAGG
AACAGCAAACGTACTCGAAGCATGCAGAGATTACGATATTCAAAAGATAGTGCATACTTCAACTTCCGAAACCTATGGTA
CAGCTCTATATGTTCCTATAGATGAGAAACACCCTATGCAGGGACAGTCACCTTATTCAGCAACAAAAATAGGTGCAGAT
AAAATAGCAGAAAGCTTTTATAGAAGCTTCAGTATGCCAATAGCAACTATAAGACCGTTTAACACATATGGCCCAAGACA
ATCTGCAAGAGCGGTAATACCAACTATAATATCACAAATTTTAGCGGGAAAAACTGAAATCAAGCTTGGAAGCTTGGCGC
CAACAAGAGACTTTAATTATGTAAAAGATACAGCAGAAGCATTTATCAAAATTGCGGAAAGTGATAAAACAGTAGGTGAA
GTTATAAATGCAGGTTCTAATTATGAAATATCTATAGGAGATACTGTTAAAAAAATTATTGATATTATTGGGAAAGATGT
AGATATATTTTGTGATGATGAAAGAATTAGACCGGAAAACAGCGAGGTTAACAGATTGTGGGCGGATAATTCCAAAATTA
AGGAACTGACAGAATGGAGGCCTCAATATAGTTTGGATTGTGGATTGAAGGAAACTGTACAGTGGATAAAGACTAATATG
CATTATTTTAAAACAGATATTTATAATGTATAA

Upstream 100 bases:

>100_bases
GGTAAAGTCAATAATGGATATTACGTTGTCAGTGCGAAAAAATAAACATGCTAGGATTTGATAAAAATCATTATGAACAT
TAAGGAAAGGAAGTTACAGG

Downstream 100 bases:

>100_bases
ATAAAATATGCAAGGAGATGAGAAAGTGATACCATTATGTATACCAGAGATTAAAGGAAATGAGTGGAAATATATAAAAG
AGTGCTTGGATACTAATTGG

Product: UDP-glucose 4-epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MNLKGKKVLVTGAEGFIGSHLTERLIELGADVTALVQYNSFNNWGWIDTFDKRVKDSINVVTGDVREYDGMKRIIKGQDV
VFHLAALIAIPYSYLSPMAYVRTNVEGTANVLEACRDYDIQKIVHTSTSETYGTALYVPIDEKHPMQGQSPYSATKIGAD
KIAESFYRSFSMPIATIRPFNTYGPRQSARAVIPTIISQILAGKTEIKLGSLAPTRDFNYVKDTAEAFIKIAESDKTVGE
VINAGSNYEISIGDTVKKIIDIIGKDVDIFCDDERIRPENSEVNRLWADNSKIKELTEWRPQYSLDCGLKETVQWIKTNM
HYFKTDIYNV

Sequences:

>Translated_330_residues
MNLKGKKVLVTGAEGFIGSHLTERLIELGADVTALVQYNSFNNWGWIDTFDKRVKDSINVVTGDVREYDGMKRIIKGQDV
VFHLAALIAIPYSYLSPMAYVRTNVEGTANVLEACRDYDIQKIVHTSTSETYGTALYVPIDEKHPMQGQSPYSATKIGAD
KIAESFYRSFSMPIATIRPFNTYGPRQSARAVIPTIISQILAGKTEIKLGSLAPTRDFNYVKDTAEAFIKIAESDKTVGE
VINAGSNYEISIGDTVKKIIDIIGKDVDIFCDDERIRPENSEVNRLWADNSKIKELTEWRPQYSLDCGLKETVQWIKTNM
HYFKTDIYNV
>Mature_330_residues
MNLKGKKVLVTGAEGFIGSHLTERLIELGADVTALVQYNSFNNWGWIDTFDKRVKDSINVVTGDVREYDGMKRIIKGQDV
VFHLAALIAIPYSYLSPMAYVRTNVEGTANVLEACRDYDIQKIVHTSTSETYGTALYVPIDEKHPMQGQSPYSATKIGAD
KIAESFYRSFSMPIATIRPFNTYGPRQSARAVIPTIISQILAGKTEIKLGSLAPTRDFNYVKDTAEAFIKIAESDKTVGE
VINAGSNYEISIGDTVKKIIDIIGKDVDIFCDDERIRPENSEVNRLWADNSKIKELTEWRPQYSLDCGLKETVQWIKTNM
HYFKTDIYNV

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=332, Percent_Identity=29.8192771084337, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI42516563, Length=335, Percent_Identity=25.3731343283582, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI56237023, Length=349, Percent_Identity=24.9283667621776, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI56118217, Length=349, Percent_Identity=24.9283667621776, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI189083684, Length=349, Percent_Identity=24.9283667621776, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI4504031, Length=340, Percent_Identity=27.6470588235294, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI193211614, Length=253, Percent_Identity=25.6916996047431, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI8393516, Length=253, Percent_Identity=25.6916996047431, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI48994969, Length=347, Percent_Identity=29.971181556196, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788353, Length=346, Percent_Identity=29.4797687861272, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786974, Length=333, Percent_Identity=26.7267267267267, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1788365, Length=346, Percent_Identity=23.4104046242775, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI1788366, Length=347, Percent_Identity=25.9365994236311, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=325, Percent_Identity=25.5384615384615, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17539532, Length=327, Percent_Identity=25.6880733944954, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI71982035, Length=361, Percent_Identity=24.6537396121884, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=353, Percent_Identity=25.2124645892351, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI133901790, Length=342, Percent_Identity=25.4385964912281, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI133901786, Length=342, Percent_Identity=25.4385964912281, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17539422, Length=342, Percent_Identity=25.4385964912281, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI133901788, Length=342, Percent_Identity=25.4385964912281, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17539424, Length=342, Percent_Identity=25.4385964912281, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=338, Percent_Identity=24.5562130177515, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI115532424, Length=334, Percent_Identity=25.748502994012, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71987463, Length=274, Percent_Identity=28.1021897810219, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17554428, Length=329, Percent_Identity=22.7963525835866, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=345, Percent_Identity=26.3768115942029, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21356223, Length=333, Percent_Identity=25.2252252252252, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19923002, Length=342, Percent_Identity=25.7309941520468, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24158427, Length=332, Percent_Identity=23.7951807228916, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013445
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46

Molecular weight: Translated: 37106; Mature: 37106

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKGKKVLVTGAEGFIGSHLTERLIELGADVTALVQYNSFNNWGWIDTFDKRVKDSINV
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHE
VTGDVREYDGMKRIIKGQDVVFHLAALIAIPYSYLSPMAYVRTNVEGTANVLEACRDYDI
EECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHCCCH
QKIVHTSTSETYGTALYVPIDEKHPMQGQSPYSATKIGADKIAESFYRSFSMPIATIRPF
HHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHCCCEEEECCC
NTYGPRQSARAVIPTIISQILAGKTEIKLGSLAPTRDFNYVKDTAEAFIKIAESDKTVGE
CCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHH
VINAGSNYEISIGDTVKKIIDIIGKDVDIFCDDERIRPENSEVNRLWADNSKIKELTEWR
HHCCCCCEEEECCHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHCC
PQYSLDCGLKETVQWIKTNMHYFKTDIYNV
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLKGKKVLVTGAEGFIGSHLTERLIELGADVTALVQYNSFNNWGWIDTFDKRVKDSINV
CCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHE
VTGDVREYDGMKRIIKGQDVVFHLAALIAIPYSYLSPMAYVRTNVEGTANVLEACRDYDI
EECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHCCCH
QKIVHTSTSETYGTALYVPIDEKHPMQGQSPYSATKIGADKIAESFYRSFSMPIATIRPF
HHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHCCCEEEECCC
NTYGPRQSARAVIPTIISQILAGKTEIKLGSLAPTRDFNYVKDTAEAFIKIAESDKTVGE
CCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHH
VINAGSNYEISIGDTVKKIIDIIGKDVDIFCDDERIRPENSEVNRLWADNSKIKELTEWR
HHCCCCCEEEECCHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHCC
PQYSLDCGLKETVQWIKTNMHYFKTDIYNV
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose

Specific reaction: dTDP-glucose = dTDP-4-dehydro-6-deoxy-D-glucose + H2O

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9141694; 9384377 [H]