Click here to switch to the map view.

The map label for this gene is argF

Identifier: 170757461

GI number: 170757461

Start: 2769570

End: 2770571

Strand: Reverse

Name: argF

Synonym: CLD_1972

Alternate gene names: 170757461

Gene position: 2770571-2769570 (Counterclockwise)

Preceding gene: 170755451

Following gene: 170757061

Centisome position: 70.0

GC content: 33.53

Gene sequence:

>1002_bases
ATGTTTAACTTAAAAAACAGAAATTTTTTAACATTAATGGATTTCACACCAAAGGAAATAAACTATTTCTTAGATTTAGC
TAGAGATTTAAAAAGAGCTAAATATACAGGAACTGAAGTACAAAGAATGAAAGGCAAAAACATTGCTTTAATATTTGAAA
AAGCTTCAACTAGAACAAGATGCGCATTTGAAGTTGGGGCAAAAGACCAAGGAGCTCATGTAACTTATTTAGGACCAACA
GGAAGTCATATTGGTAAAAAAGAATCAGCTGCAGATACTGCAAGAGTTTTAGGAAGAATGTATGACGGTATAGAATATAG
AGGATTTGGTCAAGAAATCGTTGAAACTTTAGCTGAATATGCTGGAGTACCAGTTTGGAACGGATTAACAGATGAAGACC
ACCCAACTCAAATATTAGCTGACTTCTTAACAATAAGAGAACACTTCAACAAACCATTAAACGAAATCAAATTCGCTTAT
GTTGGAGATGGTGCTAATAACATGGCAAATGCTCTTATGATAGGTGCTGTTAAAATGGGAATGGACTTTAGAATAGTTTC
TCCAAAAGAAATACCAACTGATGCTACATTAGTTGCTAAATGCAAAGAAATAGCAGCTGAAACTGGCGCTAAAGTTACAA
TAACTGATAATATCGAAGAAGGTGTTAAAGGTTGTGACGTTCTATATACAGACGTTTGGGTATCAATGGGAGAACCAGAT
TCAGTATGGGAAAGCAAAATAAAATTATTAACTCCATACAGAGTTGACATGAACATGATAAAAATGACTGGAAATCCTGA
TGCTAAATTCATGCACTGCTTACCAGCATTCCATGATGAAGAAACAGCAGTAGGTAAAGAAATAAAAGAAAAATATGGTT
TATCAGAAATGGAAGTTTCTCATGAATTATTCGAAAGCAAATACTCAATTGTATTTGATGAAGCTGAAAACAGAATGCAC
ACTATAAAAGCTGTTATGGTTGCAACATTAGGAGATCAATAA

Upstream 100 bases:

>100_bases
AGATAATTAATTAAAAAGCTAACAGGATTGCATAATACATATGTAATCTAAAATATAAAAATTTATAAAACAAATATTAT
ACTATTAGGAGGAATAACAT

Downstream 100 bases:

>100_bases
TCATTAAATATATAAGATGAAAGGAAGAATGGAAACATTCTTCCTTTTATTTTTTGATATACAAAAAAATGATAAAACAA
TTTTAGAACACTTGAAATTT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MFNLKNRNFLTLMDFTPKEINYFLDLARDLKRAKYTGTEVQRMKGKNIALIFEKASTRTRCAFEVGAKDQGAHVTYLGPT
GSHIGKKESAADTARVLGRMYDGIEYRGFGQEIVETLAEYAGVPVWNGLTDEDHPTQILADFLTIREHFNKPLNEIKFAY
VGDGANNMANALMIGAVKMGMDFRIVSPKEIPTDATLVAKCKEIAAETGAKVTITDNIEEGVKGCDVLYTDVWVSMGEPD
SVWESKIKLLTPYRVDMNMIKMTGNPDAKFMHCLPAFHDEETAVGKEIKEKYGLSEMEVSHELFESKYSIVFDEAENRMH
TIKAVMVATLGDQ

Sequences:

>Translated_333_residues
MFNLKNRNFLTLMDFTPKEINYFLDLARDLKRAKYTGTEVQRMKGKNIALIFEKASTRTRCAFEVGAKDQGAHVTYLGPT
GSHIGKKESAADTARVLGRMYDGIEYRGFGQEIVETLAEYAGVPVWNGLTDEDHPTQILADFLTIREHFNKPLNEIKFAY
VGDGANNMANALMIGAVKMGMDFRIVSPKEIPTDATLVAKCKEIAAETGAKVTITDNIEEGVKGCDVLYTDVWVSMGEPD
SVWESKIKLLTPYRVDMNMIKMTGNPDAKFMHCLPAFHDEETAVGKEIKEKYGLSEMEVSHELFESKYSIVFDEAENRMH
TIKAVMVATLGDQ
>Mature_333_residues
MFNLKNRNFLTLMDFTPKEINYFLDLARDLKRAKYTGTEVQRMKGKNIALIFEKASTRTRCAFEVGAKDQGAHVTYLGPT
GSHIGKKESAADTARVLGRMYDGIEYRGFGQEIVETLAEYAGVPVWNGLTDEDHPTQILADFLTIREHFNKPLNEIKFAY
VGDGANNMANALMIGAVKMGMDFRIVSPKEIPTDATLVAKCKEIAAETGAKVTITDNIEEGVKGCDVLYTDVWVSMGEPD
SVWESKIKLLTPYRVDMNMIKMTGNPDAKFMHCLPAFHDEETAVGKEIKEKYGLSEMEVSHELFESKYSIVFDEAENRMH
TIKAVMVATLGDQ

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=331, Percent_Identity=39.2749244712991, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI18105007, Length=338, Percent_Identity=24.8520710059172, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1790703, Length=332, Percent_Identity=59.0361445783133, Blast_Score=420, Evalue=1e-119,
Organism=Escherichia coli, GI1786469, Length=334, Percent_Identity=58.0838323353293, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI48994908, Length=374, Percent_Identity=24.5989304812834, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI2367364, Length=232, Percent_Identity=29.3103448275862, Blast_Score=72, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6322373, Length=328, Percent_Identity=32.3170731707317, Blast_Score=162, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6322331, Length=306, Percent_Identity=27.1241830065359, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24642586, Length=230, Percent_Identity=26.0869565217391, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 37279; Mature: 37279

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNLKNRNFLTLMDFTPKEINYFLDLARDLKRAKYTGTEVQRMKGKNIALIFEKASTRTR
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEECCCCCCE
CAFEVGAKDQGAHVTYLGPTGSHIGKKESAADTARVLGRMYDGIEYRGFGQEIVETLAEY
EEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
AGVPVWNGLTDEDHPTQILADFLTIREHFNKPLNEIKFAYVGDGANNMANALMIGAVKMG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCHHHHHHHHHHHHHHC
MDFRIVSPKEIPTDATLVAKCKEIAAETGAKVTITDNIEEGVKGCDVLYTDVWVSMGEPD
CCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHCCCHHHEEEEEHEEECCCCH
SVWESKIKLLTPYRVDMNMIKMTGNPDAKFMHCLPAFHDEETAVGKEIKEKYGLSEMEVS
HHHHHHHEEECCEEECCEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHH
HELFESKYSIVFDEAENRMHTIKAVMVATLGDQ
HHHHHCHHEEEEECHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFNLKNRNFLTLMDFTPKEINYFLDLARDLKRAKYTGTEVQRMKGKNIALIFEKASTRTR
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEECCCCCCE
CAFEVGAKDQGAHVTYLGPTGSHIGKKESAADTARVLGRMYDGIEYRGFGQEIVETLAEY
EEEEECCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
AGVPVWNGLTDEDHPTQILADFLTIREHFNKPLNEIKFAYVGDGANNMANALMIGAVKMG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCHHHHHHHHHHHHHHC
MDFRIVSPKEIPTDATLVAKCKEIAAETGAKVTITDNIEEGVKGCDVLYTDVWVSMGEPD
CCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHCCCHHHEEEEEHEEECCCCH
SVWESKIKLLTPYRVDMNMIKMTGNPDAKFMHCLPAFHDEETAVGKEIKEKYGLSEMEVS
HHHHHHHEEECCEEECCEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHH
HELFESKYSIVFDEAENRMHTIKAVMVATLGDQ
HHHHHCHHEEEEECHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA