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The map label for this gene is dctA [H]

Identifier: 170757367

GI number: 170757367

Start: 691725

End: 692975

Strand: Direct

Name: dctA [H]

Synonym: CLD_0185

Alternate gene names: 170757367

Gene position: 691725-692975 (Clockwise)

Preceding gene: 170755689

Following gene: 170754413

Centisome position: 17.48

GC content: 32.21

Gene sequence:

>1251_bases
ATGAAAGTATGGGAAAATTATAAATGGTCTATAATACTACTTATTTCTGTAATTATAGGAGGAATAGTTGGATTAATTAT
AGGACCAAGAGCTGAAGTAGTGGAACCTTTAGGTAATTTATTTTTAAATTTAATGTTTACTGCAATTGTACCCTTAGTAT
TTTTTAGTGTGGCTTCAGCCATTGCTAATATGGCTAATATGAAAAGATTAGGAAAGATTTTATTAAGTGTATTTATAGTA
TTTACTTTTACCGCATTAACTGCAGCTGTAATTGGTGTTATAGGAACTTTAATAATAAATCCTACAAAGGGAGTAGATCC
TTCTACTATGCAAAAGATAATGGCAAATACAGTTAACGAAGTTCAAAATAAAGAAAAGGTAACTATGTTACAACAGATTG
TAAATACAGTTACAGTATCGGATTTTGCATTATTATTTTCTAGAAAAAATATGTTACAGATTATTATATTTTCAATAATC
TTTGGTATTTCTACAGCTATGGTGGGAGAAAAGGGGAAACCTGTTGCTAAGTTTTTAGAAGCTGGTTCGACTGTAATGAT
AAAAATGATAGATATAATAATGTATACAGCACCAATAGGGCTTGGTTGTTATTTTGCTTCAGTAATAGGACAGCTTGGGC
CACAAATAATTGAAGGATATGTGAGAGTATTTATATTATATTTGGTGCTTACTTTGGTATATTATTTTATTATGTTTACT
ATATATGCATATATGGCAGCAGGAAAAACAGGAGTGCAACTATTTTGGAAGAATGCATTAGCGCCTTCAATAACATCTCT
TGCTACCTGTTCTAGTGCAGCATCTATTCCTGTTAATTTAGAAAGTACAAAAAATATGGGGGTGCCAAAGGATATTAGAG
AGACAGTTATACCCCTAGGAGCAAACACTCATAAAGATGGATCTGTTATAGGTGGGGTTTTGAAAATAACTTTTTTACTT
GGATTATTTGGTCATGATATGACTAGTTTTAAAGAAATAATTACAATAATTGTAGTGTCTTATCTAATAGGAGCTGTTAT
GGGAGCTATTCCTGGTGGAGGACTTATAGGTGCTATGCTCATCATAAGCATATATGGCTTTGCCCCTGAAGTCTTAGGAA
TAGTGGCAGTTATAAGCACTATAATAGATGCTCCAGCAACCTTATTAAATTCTACAGGAAATACAGTATGCGCTATGATG
ATAACAAGGCTTGTGGAAGGAAAAGAGTGGGCTAAAAAAATTACAGCTTAA

Upstream 100 bases:

>100_bases
AGGTAGAATTCTATAGGAAGTCAAGAATTTTTGTTATTGGGCTTGGATTATGAAAGTAAAAATAGATTTTATAAAGTTAT
ATATATTGGGAGGAGATAGA

Downstream 100 bases:

>100_bases
AAATTACTTCTTTCTTTATTCGTCTTTTAGTATACAGGATTATTTATATACTCTACTATGTACAATTCTTTCATTTTTCA
CGAAACTTGGAAATTCTAAA

Product: proton/sodium-glutamate symporter

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: C4-dicarboxylate permease [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKVWENYKWSIILLISVIIGGIVGLIIGPRAEVVEPLGNLFLNLMFTAIVPLVFFSVASAIANMANMKRLGKILLSVFIV
FTFTALTAAVIGVIGTLIINPTKGVDPSTMQKIMANTVNEVQNKEKVTMLQQIVNTVTVSDFALLFSRKNMLQIIIFSII
FGISTAMVGEKGKPVAKFLEAGSTVMIKMIDIIMYTAPIGLGCYFASVIGQLGPQIIEGYVRVFILYLVLTLVYYFIMFT
IYAYMAAGKTGVQLFWKNALAPSITSLATCSSAASIPVNLESTKNMGVPKDIRETVIPLGANTHKDGSVIGGVLKITFLL
GLFGHDMTSFKEIITIIVVSYLIGAVMGAIPGGGLIGAMLIISIYGFAPEVLGIVAVISTIIDAPATLLNSTGNTVCAMM
ITRLVEGKEWAKKITA

Sequences:

>Translated_416_residues
MKVWENYKWSIILLISVIIGGIVGLIIGPRAEVVEPLGNLFLNLMFTAIVPLVFFSVASAIANMANMKRLGKILLSVFIV
FTFTALTAAVIGVIGTLIINPTKGVDPSTMQKIMANTVNEVQNKEKVTMLQQIVNTVTVSDFALLFSRKNMLQIIIFSII
FGISTAMVGEKGKPVAKFLEAGSTVMIKMIDIIMYTAPIGLGCYFASVIGQLGPQIIEGYVRVFILYLVLTLVYYFIMFT
IYAYMAAGKTGVQLFWKNALAPSITSLATCSSAASIPVNLESTKNMGVPKDIRETVIPLGANTHKDGSVIGGVLKITFLL
GLFGHDMTSFKEIITIIVVSYLIGAVMGAIPGGGLIGAMLIISIYGFAPEVLGIVAVISTIIDAPATLLNSTGNTVCAMM
ITRLVEGKEWAKKITA
>Mature_416_residues
MKVWENYKWSIILLISVIIGGIVGLIIGPRAEVVEPLGNLFLNLMFTAIVPLVFFSVASAIANMANMKRLGKILLSVFIV
FTFTALTAAVIGVIGTLIINPTKGVDPSTMQKIMANTVNEVQNKEKVTMLQQIVNTVTVSDFALLFSRKNMLQIIIFSII
FGISTAMVGEKGKPVAKFLEAGSTVMIKMIDIIMYTAPIGLGCYFASVIGQLGPQIIEGYVRVFILYLVLTLVYYFIMFT
IYAYMAAGKTGVQLFWKNALAPSITSLATCSSAASIPVNLESTKNMGVPKDIRETVIPLGANTHKDGSVIGGVLKITFLL
GLFGHDMTSFKEIITIIVVSYLIGAVMGAIPGGGLIGAMLIISIYGFAPEVLGIVAVISTIIDAPATLLNSTGNTVCAMM
ITRLVEGKEWAKKITA

Specific function: Responsible for the transport of succinate and fumarate, but not malate, across the membrane (Probable) [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=257, Percent_Identity=22.568093385214, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI223468564, Length=263, Percent_Identity=22.4334600760456, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI223468566, Length=263, Percent_Identity=22.4334600760456, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI21314632, Length=278, Percent_Identity=23.7410071942446, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI40254478, Length=474, Percent_Identity=21.3080168776371, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI5032093, Length=272, Percent_Identity=24.6323529411765, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI194239697, Length=473, Percent_Identity=21.3530655391121, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI4827012, Length=269, Percent_Identity=23.0483271375465, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI66773030, Length=278, Percent_Identity=25.5395683453237, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI262359914, Length=209, Percent_Identity=24.4019138755981, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1789947, Length=414, Percent_Identity=25.8454106280193, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1790514, Length=393, Percent_Identity=24.4274809160305, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1788024, Length=292, Percent_Identity=25.3424657534247, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17537407, Length=431, Percent_Identity=22.5058004640371, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI193206505, Length=439, Percent_Identity=23.0068337129841, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71983106, Length=433, Percent_Identity=21.4780600461894, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71983099, Length=433, Percent_Identity=21.4780600461894, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71996953, Length=418, Percent_Identity=21.7703349282297, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI193206654, Length=412, Percent_Identity=23.3009708737864, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583025, Length=439, Percent_Identity=20.7289293849658, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI17137668, Length=439, Percent_Identity=20.7289293849658, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24583023, Length=439, Percent_Identity=20.7289293849658, Blast_Score=82, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 44640; Mature: 44640

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVWENYKWSIILLISVIIGGIVGLIIGPRAEVVEPLGNLFLNLMFTAIVPLVFFSVASA
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IANMANMKRLGKILLSVFIVFTFTALTAAVIGVIGTLIINPTKGVDPSTMQKIMANTVNE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
VQNKEKVTMLQQIVNTVTVSDFALLFSRKNMLQIIIFSIIFGISTAMVGEKGKPVAKFLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AGSTVMIKMIDIIMYTAPIGLGCYFASVIGQLGPQIIEGYVRVFILYLVLTLVYYFIMFT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYAYMAAGKTGVQLFWKNALAPSITSLATCSSAASIPVNLESTKNMGVPKDIRETVIPLG
HHHHHHCCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCEECCCCCCCCCCHHHHHHHHCCC
ANTHKDGSVIGGVLKITFLLGLFGHDMTSFKEIITIIVVSYLIGAVMGAIPGGGLIGAML
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
IISIYGFAPEVLGIVAVISTIIDAPATLLNSTGNTVCAMMITRLVEGKEWAKKITA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 10708364 [H]