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The map label for this gene is yxeI [H]

Identifier: 170757356

GI number: 170757356

Start: 2395825

End: 2396814

Strand: Reverse

Name: yxeI [H]

Synonym: CLD_2374

Alternate gene names: 170757356

Gene position: 2396814-2395825 (Counterclockwise)

Preceding gene: 170754212

Following gene: 170756319

Centisome position: 60.55

GC content: 29.39

Gene sequence:

>990_bases
ATGTGTACTAGTGTAACTTTAAAAACAAAAGATGGTAAAAATTTGTTTGGACGTACAATGGATTTTTCGGTGAAGTTTAA
TGAATCTGTTCGTTTAGTTCCTAGAAATTTCAAATGGACTAATGTAACAGATAAAGAGGAAAAAAGTACAAAATATGCTT
TTATTGGTATGTCTATAGTAACAGACAATCATCCCGTTTTTGGAGATGGTGTAAATGAAAAAGGGTTAACCTGTGCTACC
CTTTATTTTCCTGGCTTTGCAAAGTATGAAGAGGAAAATATTGAAAACAAAGAAAATATAGCTTGTTATGATTTTGTATT
ATGGGCACTATCTCAATTTAAACATTTAGAGGAAGTAAAAAAATCATTGGAAAATGTAGTAGTTATTAATAAAGTATTGT
CTATATTAAATTTTGCTCCACCATTGCATTGGATTTTATCTGATAAATCTGGTAAAAGTATAGTTGTAGAAAAAACAAAT
AGAGGATTAGAGGTATTTGATAATCCAATTGGTGTTATGACAAATAGTCCTAATTTTGAATGGCATTTAAGTAATTTACG
TCAATATATTGGAGTGAGAGCTAAACAATTTGATAATGTTACATGGAATGGTTTAGAGTTATCTGCTTTTAGCCAAGGGT
CTGGAACTTTTGGATTACCAGGAGATTTTACTCCACCCTCTCGCTTTGTTAGGGCAGTTTATTTAAAAAATAATATTGTG
GATATTGATGATGAAATTGAAGGAGTAAATGGTATATTTCATATATTATCTAATTGTGAAATTCCCAAAGGAACGGTTTT
AAAATCTGATAATGCTTGTGATTATACGTTGTACACTTCTGCAATGTGCAGTGAATCAGCTACATATTACTATCATACTT
ATGAAGATTGTCAAATTTCAGTTGTTCATTTATTTAATGAAAATTTGGATGCTCCTTTTATGAAAAACTTTCCAATTAGT
AAAGTGCAATCAATTAATAACATAAATTAA

Upstream 100 bases:

>100_bases
TGTAAAAATAATATTTAAAAGGATAATCGCATGTTATTTTTTTACAAAAACTAGCTAATGTATTAGTAATACATTGGAGT
TGAAAGGACGTGTTTAATCT

Downstream 100 bases:

>100_bases
TAACATAAAAGACTATCTTAAATTAAATTTTGAGATAGTTTTTTATAGTGTAGCCAGTAGAGGTACAACAACTTGGTGGT
GAAAGAAGGTTATTCTATAA

Product: linear amide C-N hydrolase

Products: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate; glycine

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MCTSVTLKTKDGKNLFGRTMDFSVKFNESVRLVPRNFKWTNVTDKEEKSTKYAFIGMSIVTDNHPVFGDGVNEKGLTCAT
LYFPGFAKYEEENIENKENIACYDFVLWALSQFKHLEEVKKSLENVVVINKVLSILNFAPPLHWILSDKSGKSIVVEKTN
RGLEVFDNPIGVMTNSPNFEWHLSNLRQYIGVRAKQFDNVTWNGLELSAFSQGSGTFGLPGDFTPPSRFVRAVYLKNNIV
DIDDEIEGVNGIFHILSNCEIPKGTVLKSDNACDYTLYTSAMCSESATYYYHTYEDCQISVVHLFNENLDAPFMKNFPIS
KVQSINNIN

Sequences:

>Translated_329_residues
MCTSVTLKTKDGKNLFGRTMDFSVKFNESVRLVPRNFKWTNVTDKEEKSTKYAFIGMSIVTDNHPVFGDGVNEKGLTCAT
LYFPGFAKYEEENIENKENIACYDFVLWALSQFKHLEEVKKSLENVVVINKVLSILNFAPPLHWILSDKSGKSIVVEKTN
RGLEVFDNPIGVMTNSPNFEWHLSNLRQYIGVRAKQFDNVTWNGLELSAFSQGSGTFGLPGDFTPPSRFVRAVYLKNNIV
DIDDEIEGVNGIFHILSNCEIPKGTVLKSDNACDYTLYTSAMCSESATYYYHTYEDCQISVVHLFNENLDAPFMKNFPIS
KVQSINNIN
>Mature_329_residues
MCTSVTLKTKDGKNLFGRTMDFSVKFNESVRLVPRNFKWTNVTDKEEKSTKYAFIGMSIVTDNHPVFGDGVNEKGLTCAT
LYFPGFAKYEEENIENKENIACYDFVLWALSQFKHLEEVKKSLENVVVINKVLSILNFAPPLHWILSDKSGKSIVVEKTN
RGLEVFDNPIGVMTNSPNFEWHLSNLRQYIGVRAKQFDNVTWNGLELSAFSQGSGTFGLPGDFTPPSRFVRAVYLKNNIV
DIDDEIEGVNGIFHILSNCEIPKGTVLKSDNACDYTLYTSAMCSESATYYYHTYEDCQISVVHLFNENLDAPFMKNFPIS
KVQSINNIN

Specific function: Unknown

COG id: COG3049

COG function: function code M; Penicillin V acylase and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C59 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003199 [H]

Pfam domain/function: PF02275 CBAH [H]

EC number: 3.5.1.24

Molecular weight: Translated: 37267; Mature: 37267

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTSVTLKTKDGKNLFGRTMDFSVKFNESVRLVPRNFKWTNVTDKEEKSTKYAFIGMSIV
CCCEEEEECCCCCCCCCCEEEEEEEECCCEEEEECCCEECCCCCCCCCCCEEEEEEEEEE
TDNHPVFGDGVNEKGLTCATLYFPGFAKYEEENIENKENIACYDFVLWALSQFKHLEEVK
ECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KSLENVVVINKVLSILNFAPPLHWILSDKSGKSIVVEKTNRGLEVFDNPIGVMTNSPNFE
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCEEECCCEEEEECCCCCE
WHLSNLRQYIGVRAKQFDNVTWNGLELSAFSQGSGTFGLPGDFTPPSRFVRAVYLKNNIV
EEHHHHHHHHCCHHHHCCCCEECCEEEEEECCCCCCCCCCCCCCCHHHHHEEEEEECCEE
DIDDEIEGVNGIFHILSNCEIPKGTVLKSDNACDYTLYTSAMCSESATYYYHTYEDCQIS
ECCCHHCHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEEHHHHCCCCEEEEEEECCCEEE
VVHLFNENLDAPFMKNFPISKVQSINNIN
EEEEECCCCCCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure
MCTSVTLKTKDGKNLFGRTMDFSVKFNESVRLVPRNFKWTNVTDKEEKSTKYAFIGMSIV
CCCEEEEECCCCCCCCCCEEEEEEEECCCEEEEECCCEECCCCCCCCCCCEEEEEEEEEE
TDNHPVFGDGVNEKGLTCATLYFPGFAKYEEENIENKENIACYDFVLWALSQFKHLEEVK
ECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
KSLENVVVINKVLSILNFAPPLHWILSDKSGKSIVVEKTNRGLEVFDNPIGVMTNSPNFE
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEECCCCCEEECCCEEEEECCCCCE
WHLSNLRQYIGVRAKQFDNVTWNGLELSAFSQGSGTFGLPGDFTPPSRFVRAVYLKNNIV
EEHHHHHHHHCCHHHHCCCCEECCEEEEEECCCCCCCCCCCCCCCHHHHHEEEEEECCEE
DIDDEIEGVNGIFHILSNCEIPKGTVLKSDNACDYTLYTSAMCSESATYYYHTYEDCQIS
ECCCHHCHHHHHHHHHHCCCCCCCCEEECCCCCCEEEEEHHHHCCCCEEEEEEECCCEEE
VVHLFNENLDAPFMKNFPISKVQSINNIN
EEEEECCCCCCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine; H2O

Specific reaction: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine + H2O = 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]