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The map label for this gene is pepT

Identifier: 170757296

GI number: 170757296

Start: 556965

End: 558191

Strand: Direct

Name: pepT

Synonym: CLD_0304

Alternate gene names: 170757296

Gene position: 556965-558191 (Clockwise)

Preceding gene: 170754411

Following gene: 170755656

Centisome position: 14.07

GC content: 30.97

Gene sequence:

>1227_bases
ATGAAAGATGTATTAGAAAGATTCTTGGGGTATATAAAAGTAGATACCCAATCATCAGAGGAATCTGATACTGTACCAAC
TACTAAAACTCAATTAGAGTTTGCAAAAAAATTAGGAGAAGAATTAAAGGCTATAGGACTTAAAGATGTATCTGTAGATG
AAAGCGGATATGTTATGGCTACTCTAGAATCAAACATAGATAAAAAAGTGCCTGCAATTGGCTTTATAGCACATATGGAT
ACAAGTCCAGACTTATCAGGAACTAATATTAATCCTAGAATTGTAGAGAAATATGATGGACAAGATATAGTTTTAAACAA
AGAAAAAAACATAGTGTTGAAGATTAATGAATTTCCAGAAATATTGGAATACAAAGGACAAGACATAGTAGTAACAGATG
GAAATACTCTTTTGGGAGCAGATGACAAAGCTGGAATAGCAGAGATAATTACGGCTATGGAATATCTAATAAATCATCCA
GAAATAAAACATGGAACTATAAAAGTTGGTTTTACCCCAGATGAAGAAGTGGGTAAAGGGGCAGACCACTTTGATGTTAA
AAAATTTGGAGCAGATTTAGCCTATACTTTAGATGGTGGTGGAATTGGAGAATTAGAATGTGAAACTTTTAATGCTGCAA
AAGCTAAAGTTATTATAGAAGGAAGAAATGTTCATCCAGGTTCTGCAAAAAATAAAATGACAAATGCAGTATTAGTAGCT
AATAAGTTTATAAATACGCTTCCAGAAAATGAAGTGCCAGAAAGAACAGAAGGATATGAAGGATTTTTCCATCTATTATC
TGTAAAGAGTGAAGTAGAAACAGCAGAATTAAATTATATTATAAGAGATTTTGATAGAAAAAAATTTGAAGAAAGAAAAG
AACAAATAAAAGAAGTTGGTAAAAAAATAAATGAAGAATACAATAAAGAAATAGTATGTGTAAAAGTAGAAGATCAATAT
TACAATATGAAAGAAAAAATAGATGAAGTAAAATATGTGGTAGATATAGCTTATGATGCTATGAAAGCTATTGATATTGA
ACCAATATTAGTGCCTATAAGAGGTGGTACAGATGGTTCAAGACTATCTTTTATGGGATTACCAACTCCAAATTTATTTG
CAGGTGGACATAATTTCCACGGAAGATTTGAGTTCGTTCCAGTTCTATCAATGGAAAAAGCAGCAGAACTTGTGGTTAAA
ATAGCTGAACTATATGCTAATAGATAA

Upstream 100 bases:

>100_bases
GGAGATGTTTGCATAGAAGAATAGTATATTGTAGAATATAAGTAAACAATTACAAAAAATATAAATAAAAACCAAAATTT
GTTTTAAAGGGGGATTATTA

Downstream 100 bases:

>100_bases
TAAGCTTTTTAAAATTTAGCATTGTTTTTATTAAATATAAAATATTCATAGAAAATGTCTTAAAATAAAAAAATTTAATT
TTAAGATTGCTAGAATAGAA

Product: peptidase T

Products: NA

Alternate protein names: Aminotripeptidase; Tripeptidase; Tripeptide aminopeptidase [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MKDVLERFLGYIKVDTQSSEESDTVPTTKTQLEFAKKLGEELKAIGLKDVSVDESGYVMATLESNIDKKVPAIGFIAHMD
TSPDLSGTNINPRIVEKYDGQDIVLNKEKNIVLKINEFPEILEYKGQDIVVTDGNTLLGADDKAGIAEIITAMEYLINHP
EIKHGTIKVGFTPDEEVGKGADHFDVKKFGADLAYTLDGGGIGELECETFNAAKAKVIIEGRNVHPGSAKNKMTNAVLVA
NKFINTLPENEVPERTEGYEGFFHLLSVKSEVETAELNYIIRDFDRKKFEERKEQIKEVGKKINEEYNKEIVCVKVEDQY
YNMKEKIDEVKYVVDIAYDAMKAIDIEPILVPIRGGTDGSRLSFMGLPTPNLFAGGHNFHGRFEFVPVLSMEKAAELVVK
IAELYANR

Sequences:

>Translated_408_residues
MKDVLERFLGYIKVDTQSSEESDTVPTTKTQLEFAKKLGEELKAIGLKDVSVDESGYVMATLESNIDKKVPAIGFIAHMD
TSPDLSGTNINPRIVEKYDGQDIVLNKEKNIVLKINEFPEILEYKGQDIVVTDGNTLLGADDKAGIAEIITAMEYLINHP
EIKHGTIKVGFTPDEEVGKGADHFDVKKFGADLAYTLDGGGIGELECETFNAAKAKVIIEGRNVHPGSAKNKMTNAVLVA
NKFINTLPENEVPERTEGYEGFFHLLSVKSEVETAELNYIIRDFDRKKFEERKEQIKEVGKKINEEYNKEIVCVKVEDQY
YNMKEKIDEVKYVVDIAYDAMKAIDIEPILVPIRGGTDGSRLSFMGLPTPNLFAGGHNFHGRFEFVPVLSMEKAAELVVK
IAELYANR
>Mature_408_residues
MKDVLERFLGYIKVDTQSSEESDTVPTTKTQLEFAKKLGEELKAIGLKDVSVDESGYVMATLESNIDKKVPAIGFIAHMD
TSPDLSGTNINPRIVEKYDGQDIVLNKEKNIVLKINEFPEILEYKGQDIVVTDGNTLLGADDKAGIAEIITAMEYLINHP
EIKHGTIKVGFTPDEEVGKGADHFDVKKFGADLAYTLDGGGIGELECETFNAAKAKVIIEGRNVHPGSAKNKMTNAVLVA
NKFINTLPENEVPERTEGYEGFFHLLSVKSEVETAELNYIIRDFDRKKFEERKEQIKEVGKKINEEYNKEIVCVKVEDQY
YNMKEKIDEVKYVVDIAYDAMKAIDIEPILVPIRGGTDGSRLSFMGLPTPNLFAGGHNFHGRFEFVPVLSMEKAAELVVK
IAELYANR

Specific function: Cleaves the N-terminal amino acid of tripeptides [H]

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20B family [H]

Homologues:

Organism=Escherichia coli, GI1787372, Length=409, Percent_Identity=53.5452322738386, Blast_Score=442, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR002933
- InterPro:   IPR011650
- InterPro:   IPR010161 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.11.4 [H]

Molecular weight: Translated: 45521; Mature: 45521

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDVLERFLGYIKVDTQSSEESDTVPTTKTQLEFAKKLGEELKAIGLKDVSVDESGYVMA
CHHHHHHHHCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEE
TLESNIDKKVPAIGFIAHMDTSPDLSGTNINPRIVEKYDGQDIVLNKEKNIVLKINEFPE
EECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCEEEEECCCCEEEEECCCHH
ILEYKGQDIVVTDGNTLLGADDKAGIAEIITAMEYLINHPEIKHGTIKVGFTPDEEVGKG
HHHCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHCCC
ADHFDVKKFGADLAYTLDGGGIGELECETFNAAKAKVIIEGRNVHPGSAKNKMTNAVLVA
CCCCHHHHHCCCEEEEECCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHEEHH
NKFINTLPENEVPERTEGYEGFFHLLSVKSEVETAELNYIIRDFDRKKFEERKEQIKEVG
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKINEEYNKEIVCVKVEDQYYNMKEKIDEVKYVVDIAYDAMKAIDIEPILVPIRGGTDGS
HHHHHHHCCCEEEEEECCHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
RLSFMGLPTPNLFAGGHNFHGRFEFVPVLSMEKAAELVVKIAELYANR
EEEEEECCCCCEEECCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKDVLERFLGYIKVDTQSSEESDTVPTTKTQLEFAKKLGEELKAIGLKDVSVDESGYVMA
CHHHHHHHHCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCEEEE
TLESNIDKKVPAIGFIAHMDTSPDLSGTNINPRIVEKYDGQDIVLNKEKNIVLKINEFPE
EECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCEEEEECCCCEEEEECCCHH
ILEYKGQDIVVTDGNTLLGADDKAGIAEIITAMEYLINHPEIKHGTIKVGFTPDEEVGKG
HHHCCCCEEEEECCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHCCC
ADHFDVKKFGADLAYTLDGGGIGELECETFNAAKAKVIIEGRNVHPGSAKNKMTNAVLVA
CCCCHHHHHCCCEEEEECCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHEEHH
NKFINTLPENEVPERTEGYEGFFHLLSVKSEVETAELNYIIRDFDRKKFEERKEQIKEVG
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKINEEYNKEIVCVKVEDQYYNMKEKIDEVKYVVDIAYDAMKAIDIEPILVPIRGGTDGS
HHHHHHHCCCEEEEEECCHHHCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
RLSFMGLPTPNLFAGGHNFHGRFEFVPVLSMEKAAELVVKIAELYANR
EEEEEECCCCCEEECCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA