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The map label for this gene is parE [H]

Identifier: 170757231

GI number: 170757231

Start: 2951014

End: 2952969

Strand: Reverse

Name: parE [H]

Synonym: CLD_1802

Alternate gene names: 170757231

Gene position: 2952969-2951014 (Counterclockwise)

Preceding gene: 170754595

Following gene: 170756301

Centisome position: 74.6

GC content: 30.32

Gene sequence:

>1956_bases
ATGGAGGAAAGAATAGATAACACGAAGGATTATGATGTTACCAAGTTAACCTCTTTGGAAAAATTAGAGCCTGTAAGAGT
AAGACCAGGAATGTATATAGGATCTACTGGGATAAAAGGTCTTCATCACTGTATTTGGGAGATATTAGATAATGCTATTG
ATGAAATTTCAAATGGATATGGAGATACTGCAGAGGTTATATTAAATAAAGATAAAAGCGTTACAGTTGTAGATAATGGA
AGAGGAATTCCTACAGGTATACATCCTATAAAAAAGAAGTCTGGTGTGGAAATGGTTTTTACAGAGCTTCATACAGGAGG
AAAATTTAATAATTCAATATATAAAACTTCTGGAGGACTGCATGGTGTTGGAGCATCAGTAGTTAATGCTTTATCTGAGT
GGACAGAGGTTGAAGTTTATCAGAATGATAAAATATATAAACAAAGATTTGAATATGCCTTAGATAAAAAACTAAACAAA
AAAATGCCAGGAACTCCTGTAACAAAACTTAAAATAGTAGGAGATACAGACAAAACTGGTACTAGAGTAACATTTAAACC
TGATAAAGAAGTATTTTCAACTATAGATTTTAAATTCGATGTTATAGATGAAAGACTACAAGAGTTAGCCTTTCAAAATA
AAGGGATAACTTTAAAACTTATAGACAATAGAAAAGAAGAGCCGGAAATAAAAGAATATCATTCTGAAAGAGGTCTTTTA
GACTTTATAGATTATTTAAATGAAAGTAAAGCACCAATACATAATCCAGCAGTATTATTTGAGGGAGAAAAGGAAATAAA
TAATTTAAAAATGGAAGGAGAAATATGCTTCCAATTTACAGACTCTTCAACAGAAAATATAGCAAGTTATGTAAATAATA
TTCCAACCACAGAAGCTGGAACCCATGAAACTGGATTTAAAACAGGTATGACTAGAGCTTTTAAAGAAGGAGCAAAAAAG
TTAAGCCTTATAAAAGAAAAAGATAAAGAATTTGATGGCGATGATCTTAGAGAAGGAATTACAGCTATAGTTAGAATAAA
AATAAGTAATCCTGTGTTTGAAGGTCAAACTAAAACTAAGCTTGGAAATAATGAAGCTTATACTATGATGAATGATTTAG
CCTATAGTAAATTTTTAGAATGGATAGAAGATAATAAAGAAGTATCCACATTGATTATAAATAATGCTTTAGCTGCTGCA
GCTAGAAGAGAAAAAATAAAAAAAATAAATGAAGCAGAAAAGAAAAAAATAGGTAAAGGATTAGCACCGTTAGCAGGAAA
AATAGCTGTGTGTACTTTAAGAGATTCAAATGTAACAGAGTTTATAGTGGTAGAAGGAGATTCAGCGGGAGGTTCTGCAA
AACAAGCAAGAGATAGAAGATTTCAAAGTATAATGCCTTCTAAGGGTAAAATAATGAATACAGAAAAACAAAAAATCGAA
AATGTATTAGGCAGTGAAGAATTAAAAATATTTAATACAGCTATAGGTACAGGTGTATTAGATAATTATAAAGAGGAAGA
CTTAAAATATGATAAGATAATAATATTAAGTGATGCGGATGTAGATGGATATCATATTAGAACTTTATGGATGACTTATA
TATATAGATATATGAAGTCTTTAGTAGCTAATGGTCATCTTTATATAGCTATGCCACCTTTGTATAAAGTTTACAAGAAA
GATAAGAAAGGCGAAAAGTCGGTTTATTGCTATAATAATGAAGAATTAGAAAAGGCTAAAAAACAAATTGGAAGGGGATA
CCTAATTCAAAGATATAAAGGATTAGGAGAAATGAATCCAGAACAATTATGGGAAACTACATTAAATCCTGAGACAAGAA
CACTTCAAAGAATAACTATAGAAGATGCCGCAAAAGCTGAAAAGATGGTATCTTTGCTTATGGGAGATGTAGTAGAACCT
AGAAAAAATTACTTATACAAATACGCAGAGTTTTAG

Upstream 100 bases:

>100_bases
GAATTATGTGGAATAAACTGTAAAACTTAAATAAAAATTAAAGAATATGGTTACTACTAAGAATTAATATAAACATTAAG
TATAGATTTGGAGGATAAAA

Downstream 100 bases:

>100_bases
ATAAAAGGGCTAAATAATATATATTTAGCCTTTGCCATGTAAATAAATATAAAAGCACAGGGAGGACTTTTGGATGGCTA
AAAAAATAGTAATACCTAAA

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 651; Mature: 651

Protein sequence:

>651_residues
MEERIDNTKDYDVTKLTSLEKLEPVRVRPGMYIGSTGIKGLHHCIWEILDNAIDEISNGYGDTAEVILNKDKSVTVVDNG
RGIPTGIHPIKKKSGVEMVFTELHTGGKFNNSIYKTSGGLHGVGASVVNALSEWTEVEVYQNDKIYKQRFEYALDKKLNK
KMPGTPVTKLKIVGDTDKTGTRVTFKPDKEVFSTIDFKFDVIDERLQELAFQNKGITLKLIDNRKEEPEIKEYHSERGLL
DFIDYLNESKAPIHNPAVLFEGEKEINNLKMEGEICFQFTDSSTENIASYVNNIPTTEAGTHETGFKTGMTRAFKEGAKK
LSLIKEKDKEFDGDDLREGITAIVRIKISNPVFEGQTKTKLGNNEAYTMMNDLAYSKFLEWIEDNKEVSTLIINNALAAA
ARREKIKKINEAEKKKIGKGLAPLAGKIAVCTLRDSNVTEFIVVEGDSAGGSAKQARDRRFQSIMPSKGKIMNTEKQKIE
NVLGSEELKIFNTAIGTGVLDNYKEEDLKYDKIIILSDADVDGYHIRTLWMTYIYRYMKSLVANGHLYIAMPPLYKVYKK
DKKGEKSVYCYNNEELEKAKKQIGRGYLIQRYKGLGEMNPEQLWETTLNPETRTLQRITIEDAAKAEKMVSLLMGDVVEP
RKNYLYKYAEF

Sequences:

>Translated_651_residues
MEERIDNTKDYDVTKLTSLEKLEPVRVRPGMYIGSTGIKGLHHCIWEILDNAIDEISNGYGDTAEVILNKDKSVTVVDNG
RGIPTGIHPIKKKSGVEMVFTELHTGGKFNNSIYKTSGGLHGVGASVVNALSEWTEVEVYQNDKIYKQRFEYALDKKLNK
KMPGTPVTKLKIVGDTDKTGTRVTFKPDKEVFSTIDFKFDVIDERLQELAFQNKGITLKLIDNRKEEPEIKEYHSERGLL
DFIDYLNESKAPIHNPAVLFEGEKEINNLKMEGEICFQFTDSSTENIASYVNNIPTTEAGTHETGFKTGMTRAFKEGAKK
LSLIKEKDKEFDGDDLREGITAIVRIKISNPVFEGQTKTKLGNNEAYTMMNDLAYSKFLEWIEDNKEVSTLIINNALAAA
ARREKIKKINEAEKKKIGKGLAPLAGKIAVCTLRDSNVTEFIVVEGDSAGGSAKQARDRRFQSIMPSKGKIMNTEKQKIE
NVLGSEELKIFNTAIGTGVLDNYKEEDLKYDKIIILSDADVDGYHIRTLWMTYIYRYMKSLVANGHLYIAMPPLYKVYKK
DKKGEKSVYCYNNEELEKAKKQIGRGYLIQRYKGLGEMNPEQLWETTLNPETRTLQRITIEDAAKAEKMVSLLMGDVVEP
RKNYLYKYAEF
>Mature_651_residues
MEERIDNTKDYDVTKLTSLEKLEPVRVRPGMYIGSTGIKGLHHCIWEILDNAIDEISNGYGDTAEVILNKDKSVTVVDNG
RGIPTGIHPIKKKSGVEMVFTELHTGGKFNNSIYKTSGGLHGVGASVVNALSEWTEVEVYQNDKIYKQRFEYALDKKLNK
KMPGTPVTKLKIVGDTDKTGTRVTFKPDKEVFSTIDFKFDVIDERLQELAFQNKGITLKLIDNRKEEPEIKEYHSERGLL
DFIDYLNESKAPIHNPAVLFEGEKEINNLKMEGEICFQFTDSSTENIASYVNNIPTTEAGTHETGFKTGMTRAFKEGAKK
LSLIKEKDKEFDGDDLREGITAIVRIKISNPVFEGQTKTKLGNNEAYTMMNDLAYSKFLEWIEDNKEVSTLIINNALAAA
ARREKIKKINEAEKKKIGKGLAPLAGKIAVCTLRDSNVTEFIVVEGDSAGGSAKQARDRRFQSIMPSKGKIMNTEKQKIE
NVLGSEELKIFNTAIGTGVLDNYKEEDLKYDKIIILSDADVDGYHIRTLWMTYIYRYMKSLVANGHLYIAMPPLYKVYKK
DKKGEKSVYCYNNEELEKAKKQIGRGYLIQRYKGLGEMNPEQLWETTLNPETRTLQRITIEDAAKAEKMVSLLMGDVVEP
RKNYLYKYAEF

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=622, Percent_Identity=26.3665594855305, Blast_Score=123, Evalue=7e-28,
Organism=Homo sapiens, GI19913406, Length=647, Percent_Identity=23.6476043276662, Blast_Score=118, Evalue=2e-26,
Organism=Escherichia coli, GI48994957, Length=580, Percent_Identity=41.0344827586207, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1789408, Length=651, Percent_Identity=36.7127496159754, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17535065, Length=677, Percent_Identity=24.6676514032496, Blast_Score=138, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI212645845, Length=549, Percent_Identity=26.5938069216758, Blast_Score=122, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6324241, Length=647, Percent_Identity=25.193199381762, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17136538, Length=625, Percent_Identity=26.08, Blast_Score=121, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005740 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.3

Molecular weight: Translated: 73759; Mature: 73759

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERIDNTKDYDVTKLTSLEKLEPVRVRPGMYIGSTGIKGLHHCIWEILDNAIDEISNGY
CCCCCCCCCCCCHHHHCCHHHCCCEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
GDTAEVILNKDKSVTVVDNGRGIPTGIHPIKKKSGVEMVFTELHTGGKFNNSIYKTSGGL
CCCCHHEEECCCEEEEEECCCCCCCCCCCHHCCCCHHEEEEHHHCCCCCCCCEEECCCCC
HGVGASVVNALSEWTEVEVYQNDKIYKQRFEYALDKKLNKKMPGTPVTKLKIVGDTDKTG
CCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCC
TRVTFKPDKEVFSTIDFKFDVIDERLQELAFQNKGITLKLIDNRKEEPEIKEYHSERGLL
CEEEECCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCHH
DFIDYLNESKAPIHNPAVLFEGEKEINNLKMEGEICFQFTDSSTENIASYVNNIPTTEAG
HHHHHHCCCCCCCCCCEEEEECCHHHCCEEECCEEEEEECCCCHHHHHHHHHCCCCCCCC
THETGFKTGMTRAFKEGAKKLSLIKEKDKEFDGDDLREGITAIVRIKISNPVFEGQTKTK
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEEEEEEECCCEECCCCCCC
LGNNEAYTMMNDLAYSKFLEWIEDNKEVSTLIINNALAAAARREKIKKINEAEKKKIGKG
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LAPLAGKIAVCTLRDSNVTEFIVVEGDSAGGSAKQARDRRFQSIMPSKGKIMNTEKQKIE
CCCCCCCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEECCHHHHHH
NVLGSEELKIFNTAIGTGVLDNYKEEDLKYDKIIILSDADVDGYHIRTLWMTYIYRYMKS
HHHCCCHHEEHHHHHHCCHHCCCHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
LVANGHLYIAMPPLYKVYKKDKKGEKSVYCYNNEELEKAKKQIGRGYLIQRYKGLGEMNP
HHCCCEEEEEECHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHCCCCCCH
EQLWETTLNPETRTLQRITIEDAAKAEKMVSLLMGDVVEPRKNYLYKYAEF
HHHHHHCCCCCHHHHHEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure
MEERIDNTKDYDVTKLTSLEKLEPVRVRPGMYIGSTGIKGLHHCIWEILDNAIDEISNGY
CCCCCCCCCCCCHHHHCCHHHCCCEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
GDTAEVILNKDKSVTVVDNGRGIPTGIHPIKKKSGVEMVFTELHTGGKFNNSIYKTSGGL
CCCCHHEEECCCEEEEEECCCCCCCCCCCHHCCCCHHEEEEHHHCCCCCCCCEEECCCCC
HGVGASVVNALSEWTEVEVYQNDKIYKQRFEYALDKKLNKKMPGTPVTKLKIVGDTDKTG
CCHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCC
TRVTFKPDKEVFSTIDFKFDVIDERLQELAFQNKGITLKLIDNRKEEPEIKEYHSERGLL
CEEEECCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCHH
DFIDYLNESKAPIHNPAVLFEGEKEINNLKMEGEICFQFTDSSTENIASYVNNIPTTEAG
HHHHHHCCCCCCCCCCEEEEECCHHHCCEEECCEEEEEECCCCHHHHHHHHHCCCCCCCC
THETGFKTGMTRAFKEGAKKLSLIKEKDKEFDGDDLREGITAIVRIKISNPVFEGQTKTK
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHEEEEEEEECCCEECCCCCCC
LGNNEAYTMMNDLAYSKFLEWIEDNKEVSTLIINNALAAAARREKIKKINEAEKKKIGKG
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LAPLAGKIAVCTLRDSNVTEFIVVEGDSAGGSAKQARDRRFQSIMPSKGKIMNTEKQKIE
CCCCCCCEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEECCHHHHHH
NVLGSEELKIFNTAIGTGVLDNYKEEDLKYDKIIILSDADVDGYHIRTLWMTYIYRYMKS
HHHCCCHHEEHHHHHHCCHHCCCHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
LVANGHLYIAMPPLYKVYKKDKKGEKSVYCYNNEELEKAKKQIGRGYLIQRYKGLGEMNP
HHCCCEEEEEECHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHCCCCCCH
EQLWETTLNPETRTLQRITIEDAAKAEKMVSLLMGDVVEPRKNYLYKYAEF
HHHHHHCCCCCHHHHHEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerization [C]

Inhibitor: CJ-12, 371; CJ-12, 372; Coumermycin; Doxorubicin; Sitafloxacin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969507; 9539793; 9384377 [H]