Click here to switch to the map view.

The map label for this gene is rlmN [H]

Identifier: 170757229

GI number: 170757229

Start: 2629755

End: 2630783

Strand: Reverse

Name: rlmN [H]

Synonym: CLD_2131

Alternate gene names: 170757229

Gene position: 2630783-2629755 (Counterclockwise)

Preceding gene: 170756128

Following gene: 170754821

Centisome position: 66.46

GC content: 27.21

Gene sequence:

>1029_bases
ATGGAAAACATATTAGACTTTACATTAGAAGAACTAAAAGAATGGCTAATATCTAAAGAAGAAAAGGCTTTTAGAGCAAA
ACAAGTTTTTGATTGGATTTATAATAAACTTATTTTTGATTTTAATAATATGAAAAACATACCTTATAAAACTAAGAATT
TGCTTTCTGATAATTTTTATATTGGTGTACCAAAAGTAGTTAAAAAATTAATGTCACAGGATAAAAACACCTATAAATTT
TTATTTGAGTATAAAGATGGAAATATAATAGAATCTGTGGTGATGAAATATAAACATGGTAATTCTATTTGTGTATCTAC
TCAAGTTGGATGTAGAATGGGCTGCAAATTTTGTGCATCTACTTTAGATGGCGTTATAAGAAATCTAACTAGTGGAGAAA
TATTATCTCAGATAATGGCAGCACAAAAAGAAATAGGTGAAAGAATTTCTAATGTGGTTTTAATGGGAAGTGGAGAACCT
TTAGATAATTTTGAAAATGTGACTAAATTTTTAGATTTAGTTACATCTGATACTACACTAAATATAGGACAAAGACATAT
AACCCTATCTACTTGTGGTATAGTACCCAAAATAAAAGAATTAGCAGACAAGAATTATAATATAACTTTGGCCATATCCC
TGCATTCACCAGAAGATTTATTAAGAAAAGAAATGATGCCCATAGCTAATAAATATTCTATAAAAGAGCTTATGGAAGCT
TGTGATTATTATATAAATAAAACTAACAGAAGAATAACTTTTGAATATGCATTAGTAAAAGGTAAAAATGATTCTATCAA
AGAAGCTAAAAAACTAAGTACAGTGTTAAAAGGAAAACTTTGTCATGTAAACTTAATCCCTGTAAATGAAATAAAAGAAA
ATAGCTATGAAAAATCCACATTAAAAAATATAGAATCCTTTGGTAATATTTTAAAAGAGAATGGGATAGAAACAACTATA
AGAAGAGAAATGGGGGCAGATATAAATGCTGCCTGTGGACAACTAAGAAGAAGTTATGTTTCAAAATAG

Upstream 100 bases:

>100_bases
ATATAATATATCATAAAGAAGGATTTGTATCCATATTGCCTAATGATAAAATGGATGGCTTTTTTATAGCTAAATTGAAG
AAATGTTAGTAGGTGTTAAG

Downstream 100 bases:

>100_bases
AGCAAAGGGGTGGAAAATATGTTGGGGATACTATCTGATATAGGTAATGTTAGAGAGATAAATGAAGACTTTGTAGATTA
TTATCAAGGAGACCGATTTA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MENILDFTLEELKEWLISKEEKAFRAKQVFDWIYNKLIFDFNNMKNIPYKTKNLLSDNFYIGVPKVVKKLMSQDKNTYKF
LFEYKDGNIIESVVMKYKHGNSICVSTQVGCRMGCKFCASTLDGVIRNLTSGEILSQIMAAQKEIGERISNVVLMGSGEP
LDNFENVTKFLDLVTSDTTLNIGQRHITLSTCGIVPKIKELADKNYNITLAISLHSPEDLLRKEMMPIANKYSIKELMEA
CDYYINKTNRRITFEYALVKGKNDSIKEAKKLSTVLKGKLCHVNLIPVNEIKENSYEKSTLKNIESFGNILKENGIETTI
RREMGADINAACGQLRRSYVSK

Sequences:

>Translated_342_residues
MENILDFTLEELKEWLISKEEKAFRAKQVFDWIYNKLIFDFNNMKNIPYKTKNLLSDNFYIGVPKVVKKLMSQDKNTYKF
LFEYKDGNIIESVVMKYKHGNSICVSTQVGCRMGCKFCASTLDGVIRNLTSGEILSQIMAAQKEIGERISNVVLMGSGEP
LDNFENVTKFLDLVTSDTTLNIGQRHITLSTCGIVPKIKELADKNYNITLAISLHSPEDLLRKEMMPIANKYSIKELMEA
CDYYINKTNRRITFEYALVKGKNDSIKEAKKLSTVLKGKLCHVNLIPVNEIKENSYEKSTLKNIESFGNILKENGIETTI
RREMGADINAACGQLRRSYVSK
>Mature_342_residues
MENILDFTLEELKEWLISKEEKAFRAKQVFDWIYNKLIFDFNNMKNIPYKTKNLLSDNFYIGVPKVVKKLMSQDKNTYKF
LFEYKDGNIIESVVMKYKHGNSICVSTQVGCRMGCKFCASTLDGVIRNLTSGEILSQIMAAQKEIGERISNVVLMGSGEP
LDNFENVTKFLDLVTSDTTLNIGQRHITLSTCGIVPKIKELADKNYNITLAISLHSPEDLLRKEMMPIANKYSIKELMEA
CDYYINKTNRRITFEYALVKGKNDSIKEAKKLSTVLKGKLCHVNLIPVNEIKENSYEKSTLKNIESFGNILKENGIETTI
RREMGADINAACGQLRRSYVSK

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=343, Percent_Identity=37.0262390670554, Blast_Score=228, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 39043; Mature: 39043

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENILDFTLEELKEWLISKEEKAFRAKQVFDWIYNKLIFDFNNMKNIPYKTKNLLSDNFY
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCE
IGVPKVVKKLMSQDKNTYKFLFEYKDGNIIESVVMKYKHGNSICVSTQVGCRMGCKFCAS
ECHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHH
TLDGVIRNLTSGEILSQIMAAQKEIGERISNVVLMGSGEPLDNFENVTKFLDLVTSDTTL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHHHCCCEE
NIGQRHITLSTCGIVPKIKELADKNYNITLAISLHSPEDLLRKEMMPIANKYSIKELMEA
ECCCEEEEEEHHCCCHHHHHHCCCCCEEEEEEEECCHHHHHHHHHCCCCCHHHHHHHHHH
CDYYINKTNRRITFEYALVKGKNDSIKEAKKLSTVLKGKLCHVNLIPVNEIKENSYEKST
HHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEHHHHHCCCHHHHH
LKNIESFGNILKENGIETTIRREMGADINAACGQLRRSYVSK
HHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MENILDFTLEELKEWLISKEEKAFRAKQVFDWIYNKLIFDFNNMKNIPYKTKNLLSDNFY
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCE
IGVPKVVKKLMSQDKNTYKFLFEYKDGNIIESVVMKYKHGNSICVSTQVGCRMGCKFCAS
ECHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHH
TLDGVIRNLTSGEILSQIMAAQKEIGERISNVVLMGSGEPLDNFENVTKFLDLVTSDTTL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHHHHHCCCEE
NIGQRHITLSTCGIVPKIKELADKNYNITLAISLHSPEDLLRKEMMPIANKYSIKELMEA
ECCCEEEEEEHHCCCHHHHHHCCCCCEEEEEEEECCHHHHHHHHHCCCCCHHHHHHHHHH
CDYYINKTNRRITFEYALVKGKNDSIKEAKKLSTVLKGKLCHVNLIPVNEIKENSYEKST
HHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEHHHHHCCCHHHHH
LKNIESFGNILKENGIETTIRREMGADINAACGQLRRSYVSK
HHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA