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The map label for this gene is yfiJ [H]

Identifier: 170757149

GI number: 170757149

Start: 1541180

End: 1542337

Strand: Direct

Name: yfiJ [H]

Synonym: CLD_3160

Alternate gene names: 170757149

Gene position: 1541180-1542337 (Clockwise)

Preceding gene: 170755862

Following gene: 170756196

Centisome position: 38.94

GC content: 25.91

Gene sequence:

>1158_bases
ATGGAGAGAAAAAAATTACTAATTATATTTAGATACATAGCTATATTATTTCTTATATTTGGTATAGCTTCTTTTAAAAA
AGAAAATATTACAACTAATACAGTTATTCTAATATTATTATTTATTATAAATAATCAAATTAGATTTTTTGTTTTAAATA
AAAATAAGAATTTAGTTTTGATTTCTATAATATTAGAATGTATTTTAGCTTACATTGGATATATTAATTATTCTGGAATA
TTGTTTTTTTATTTTTTTACAGATATTTTGGATTCAGCTCTTTTTCTTAAAGATGAGGTGTCTTACTTAGCCTGTTCTAT
AATATTAATTATAGTCATATTTATCGGATGGAATTTAAATTTAAGCGAAATGTTATCTATAATAACTTCTTTTATTATGT
TGTTTATATTAGCTACTTATATAAAAGAAGAAAATAGTGGTAAATTAAGGGCGCAAAAATTATATGATAAACTTAGAATC
TGGGAAGAGAAATTAAAAAAAGCTAATAAGGACTTAGAGAGTTATGCTAATTCTATAGAAGAGCTTACAGTTCTCCGTGA
AAGAAATAGAATATCCAGAGAAATACATGATAGTGTAGGACATAGTTTATCTACAATGATAATACAATTAGGAGCTATAG
AAAAGATTGCTGAAAAGGATGGGAAACTCACATCAGAAATGGCAAGAAATTTAGGTGAGTTTGCTAAAAATAGTTTAGGA
GAAGTAAGGTGTGCTGTAAGAGCATTAAAACCTAGAGAATTTGAAAAATATGAAGGTATATTAGCAATAGAAGAGCTTAC
AAAGAATTTTGAAAAACTTACAGGGGTAAAAGTAAAATTGGGATTTTCAAAAGAAAAATGGACTCTTAATTCAGATCAAT
GTTTTGTTTTATATAGGGTAACTCAGGAATTCTTATCCAACTCTTTAAGACATGGAAACGCCACAAGAGTGGATATATTT
ATGGGGTTTAATGAAGAAAATTTAATAATAACTCTTAAGGATAATGGACAAGGGGTGGATAAAATAGAAAAAGGTGTAGG
CCTTAAAAGTATATGGGAAAGAGTTAATGAATTAGGTGGAACTGTAGCATATAATAGTAAAAAAGAAGAAGGATTTTTAT
TAAAAGTAGTATTATATCCACAAATTAATGAAACCTAA

Upstream 100 bases:

>100_bases
TAGTTTATAATATAAATAATAGGGATAGTTTATTACAAATTAGTTTTTAAAGACTAAGTAATAAACTGTCCTTATTTAAA
AATAATAAAGGATTAAAAAT

Downstream 100 bases:

>100_bases
TAATTTTCATTATAAAGCCCATGGAAAATTAATTTAATAAGGGGAAAAATAAAATGAATAAAACTAATATTATAGTAGCA
GATGATGAAAAATTAATAAG

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MERKKLLIIFRYIAILFLIFGIASFKKENITTNTVILILLFIINNQIRFFVLNKNKNLVLISIILECILAYIGYINYSGI
LFFYFFTDILDSALFLKDEVSYLACSIILIIVIFIGWNLNLSEMLSIITSFIMLFILATYIKEENSGKLRAQKLYDKLRI
WEEKLKKANKDLESYANSIEELTVLRERNRISREIHDSVGHSLSTMIIQLGAIEKIAEKDGKLTSEMARNLGEFAKNSLG
EVRCAVRALKPREFEKYEGILAIEELTKNFEKLTGVKVKLGFSKEKWTLNSDQCFVLYRVTQEFLSNSLRHGNATRVDIF
MGFNEENLIITLKDNGQGVDKIEKGVGLKSIWERVNELGGTVAYNSKKEEGFLLKVVLYPQINET

Sequences:

>Translated_385_residues
MERKKLLIIFRYIAILFLIFGIASFKKENITTNTVILILLFIINNQIRFFVLNKNKNLVLISIILECILAYIGYINYSGI
LFFYFFTDILDSALFLKDEVSYLACSIILIIVIFIGWNLNLSEMLSIITSFIMLFILATYIKEENSGKLRAQKLYDKLRI
WEEKLKKANKDLESYANSIEELTVLRERNRISREIHDSVGHSLSTMIIQLGAIEKIAEKDGKLTSEMARNLGEFAKNSLG
EVRCAVRALKPREFEKYEGILAIEELTKNFEKLTGVKVKLGFSKEKWTLNSDQCFVLYRVTQEFLSNSLRHGNATRVDIF
MGFNEENLIITLKDNGQGVDKIEKGVGLKSIWERVNELGGTVAYNSKKEEGFLLKVVLYPQINET
>Mature_385_residues
MERKKLLIIFRYIAILFLIFGIASFKKENITTNTVILILLFIINNQIRFFVLNKNKNLVLISIILECILAYIGYINYSGI
LFFYFFTDILDSALFLKDEVSYLACSIILIIVIFIGWNLNLSEMLSIITSFIMLFILATYIKEENSGKLRAQKLYDKLRI
WEEKLKKANKDLESYANSIEELTVLRERNRISREIHDSVGHSLSTMIIQLGAIEKIAEKDGKLTSEMARNLGEFAKNSLG
EVRCAVRALKPREFEKYEGILAIEELTKNFEKLTGVKVKLGFSKEKWTLNSDQCFVLYRVTQEFLSNSLRHGNATRVDIF
MGFNEENLIITLKDNGQGVDKIEKGVGLKSIWERVNELGGTVAYNSKKEEGFLLKVVLYPQINET

Specific function: Member of the two-component regulatory system yfiJ/yfiK. Probably activates yfiK by phosphorylation [H]

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=222, Percent_Identity=26.1261261261261, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082311, Length=184, Percent_Identity=27.1739130434783, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788812, Length=201, Percent_Identity=21.8905472636816, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011712 [H]

Pfam domain/function: PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44300; Mature: 44300

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERKKLLIIFRYIAILFLIFGIASFKKENITTNTVILILLFIINNQIRFFVLNKNKNLVL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEH
ISIILECILAYIGYINYSGILFFYFFTDILDSALFLKDEVSYLACSIILIIVIFIGWNLN
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LSEMLSIITSFIMLFILATYIKEENSGKLRAQKLYDKLRIWEEKLKKANKDLESYANSIE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELTVLRERNRISREIHDSVGHSLSTMIIQLGAIEKIAEKDGKLTSEMARNLGEFAKNSLG
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EVRCAVRALKPREFEKYEGILAIEELTKNFEKLTGVKVKLGFSKEKWTLNSDQCFVLYRV
HHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHCCEEEEECCCCCCEEECCCCEEEEHHH
TQEFLSNSLRHGNATRVDIFMGFNEENLIITLKDNGQGVDKIEKGVGLKSIWERVNELGG
HHHHHHHHHCCCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHCC
TVAYNSKKEEGFLLKVVLYPQINET
EEEECCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MERKKLLIIFRYIAILFLIFGIASFKKENITTNTVILILLFIINNQIRFFVLNKNKNLVL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEEECCCCEEH
ISIILECILAYIGYINYSGILFFYFFTDILDSALFLKDEVSYLACSIILIIVIFIGWNLN
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LSEMLSIITSFIMLFILATYIKEENSGKLRAQKLYDKLRIWEEKLKKANKDLESYANSIE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELTVLRERNRISREIHDSVGHSLSTMIIQLGAIEKIAEKDGKLTSEMARNLGEFAKNSLG
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
EVRCAVRALKPREFEKYEGILAIEELTKNFEKLTGVKVKLGFSKEKWTLNSDQCFVLYRV
HHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHCCEEEEECCCCCCEEECCCCEEEEHHH
TQEFLSNSLRHGNATRVDIFMGFNEENLIITLKDNGQGVDKIEKGVGLKSIWERVNELGG
HHHHHHHHHCCCCCEEEEEEEECCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHCC
TVAYNSKKEEGFLLKVVLYPQINET
EEEECCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]