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The map label for this gene is maeB [C]

Identifier: 170757093

GI number: 170757093

Start: 251895

End: 253067

Strand: Direct

Name: maeB [C]

Synonym: CLD_0565

Alternate gene names: 170757093

Gene position: 251895-253067 (Clockwise)

Preceding gene: 170756278

Following gene: 170757704

Centisome position: 6.36

GC content: 31.03

Gene sequence:

>1173_bases
ATGGATATCAAAGAAAAATCACTAATGGTACATAAAAAATTTAAAGGCAAATTATCTATAGAAGGAAAAATACAAGTAAA
AAATAAAGAAGATTTATCTATAGCATATACTCCAGGAGTTGCAGAACCATGCGTAAAAATAAATGAAGATAAATCTTTAG
TTTATGAATATACAATGAAGGGAAATACAGTAGCTGTAGTAACTAATGGTACAGCAGTTTTAGGTTTAGGGGATATAGGG
CCTTATGCAGGATTGCCTGTTATGGAAGGAAAAGCTCTTTTATTTAAGGAATTTGCAGATATAGATTCATTTCCAATTTG
TATAGATAGTAAAGACCCTGAAGAAATTATAAAAACAGTTAAACTTATAGCTCCAGGATTTGGAGGAATAAATTTAGAGG
ACATAAAAGCACCAGAATGTTTTTATATAGAAAAAAAATTAAAAGAAGAATTAGATATTCCAGTGTTTCATGATGATCAG
CATGGAACAGCTATAGTTGTATTAGCAGGAATATATAATGCTTTAAGACTTGTAGGAAAAAAATTAGAAGAAGCAAAAAT
AGTTATAAATGGTGCTGGCTCTGCGGGAATATCCATATGTAAATTGTTATTACAAGCAGGTGCTAAAAGCATAATAATGT
GTGATAAAGAAGGGTCTTTAGTAAAAGGTAATAGTAATTTAAATGAAGCACAAAAATTAATAGTTGAAGTAACAAATAAG
GAAAATGAAAAAGGAACATTAAAAGACGTAATAAAAGGAAAAGATGTATTTATAGGTGTATCAGCCCCAAATATACTTAC
AGAAGAAATGGTAACCTCTATGAACAAAGATTCCATAGTATTTGCTATGGCAAACCCAACTCCAGAAATAATGCCGGATA
AAGCAAAAAAGGCAGGAGCTAGAGTAGTGGCTACAGGAAGATCCGATTTCCCAAATCAAATAAATAATGTGTTAGTATTC
CCAGGAATTTTTAGAGGAGCATTAGATGTAAGATCTAAAGTTATAAATGAGGAAATGAAACTAGCAGCATCTAAAGCTAT
AGCCTCTTTGGTACAGGATAATGAATTAAATGAAGAGTACATAATACCAGGAGCCTTTGATAAAAGAGTAGCAGAAGTTG
TGGCAGAAGAAGTAAAAAAAGTAGCCCTAAAAATGAGACTATCAAAATTATAA

Upstream 100 bases:

>100_bases
ACTCCCACTTTGAAGAAGATGGGAGTATTAGAGCGGGTAGTTATTGGATAAATTAAATAAATCCTAGATACCTATATACA
ATGAAAGAGGGGATAATAAT

Downstream 100 bases:

>100_bases
CTTTTTAAAGGTATTAAAACAAGAAATGCTTTTAGAGAAAATAGCTATCTATTGAGCAAAAGTATTTCTTGTTTTAATTC
TCAACCTTTAAAGGGTGTTA

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MDIKEKSLMVHKKFKGKLSIEGKIQVKNKEDLSIAYTPGVAEPCVKINEDKSLVYEYTMKGNTVAVVTNGTAVLGLGDIG
PYAGLPVMEGKALLFKEFADIDSFPICIDSKDPEEIIKTVKLIAPGFGGINLEDIKAPECFYIEKKLKEELDIPVFHDDQ
HGTAIVVLAGIYNALRLVGKKLEEAKIVINGAGSAGISICKLLLQAGAKSIIMCDKEGSLVKGNSNLNEAQKLIVEVTNK
ENEKGTLKDVIKGKDVFIGVSAPNILTEEMVTSMNKDSIVFAMANPTPEIMPDKAKKAGARVVATGRSDFPNQINNVLVF
PGIFRGALDVRSKVINEEMKLAASKAIASLVQDNELNEEYIIPGAFDKRVAEVVAEEVKKVALKMRLSKL

Sequences:

>Translated_390_residues
MDIKEKSLMVHKKFKGKLSIEGKIQVKNKEDLSIAYTPGVAEPCVKINEDKSLVYEYTMKGNTVAVVTNGTAVLGLGDIG
PYAGLPVMEGKALLFKEFADIDSFPICIDSKDPEEIIKTVKLIAPGFGGINLEDIKAPECFYIEKKLKEELDIPVFHDDQ
HGTAIVVLAGIYNALRLVGKKLEEAKIVINGAGSAGISICKLLLQAGAKSIIMCDKEGSLVKGNSNLNEAQKLIVEVTNK
ENEKGTLKDVIKGKDVFIGVSAPNILTEEMVTSMNKDSIVFAMANPTPEIMPDKAKKAGARVVATGRSDFPNQINNVLVF
PGIFRGALDVRSKVINEEMKLAASKAIASLVQDNELNEEYIIPGAFDKRVAEVVAEEVKKVALKMRLSKL
>Mature_390_residues
MDIKEKSLMVHKKFKGKLSIEGKIQVKNKEDLSIAYTPGVAEPCVKINEDKSLVYEYTMKGNTVAVVTNGTAVLGLGDIG
PYAGLPVMEGKALLFKEFADIDSFPICIDSKDPEEIIKTVKLIAPGFGGINLEDIKAPECFYIEKKLKEELDIPVFHDDQ
HGTAIVVLAGIYNALRLVGKKLEEAKIVINGAGSAGISICKLLLQAGAKSIIMCDKEGSLVKGNSNLNEAQKLIVEVTNK
ENEKGTLKDVIKGKDVFIGVSAPNILTEEMVTSMNKDSIVFAMANPTPEIMPDKAKKAGARVVATGRSDFPNQINNVLVF
PGIFRGALDVRSKVINEEMKLAASKAIASLVQDNELNEEYIIPGAFDKRVAEVVAEEVKKVALKMRLSKL

Specific function: In addition to the NAD-dependent oxidative decarboxylation of L-malate, the enzyme catalyzes the decarboxylation of oxaloacetate [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=411, Percent_Identity=26.5206812652068, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI62420880, Length=411, Percent_Identity=26.5206812652068, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI239049447, Length=411, Percent_Identity=26.5206812652068, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI4505145, Length=411, Percent_Identity=27.4939172749392, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI4505143, Length=359, Percent_Identity=27.0194986072423, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI270265879, Length=370, Percent_Identity=27.8378378378378, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1788806, Length=406, Percent_Identity=48.5221674876847, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI87081919, Length=481, Percent_Identity=25.3638253638254, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17537199, Length=450, Percent_Identity=26.4444444444444, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6322823, Length=345, Percent_Identity=26.9565217391304, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356279, Length=416, Percent_Identity=27.6442307692308, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281362674, Length=416, Percent_Identity=27.6442307692308, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281362672, Length=416, Percent_Identity=27.6442307692308, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI78707242, Length=407, Percent_Identity=25.7985257985258, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI78707238, Length=407, Percent_Identity=25.7985257985258, Blast_Score=98, Evalue=8e-21,
Organism=Drosophila melanogaster, GI78707240, Length=407, Percent_Identity=25.7985257985258, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI78707232, Length=373, Percent_Identity=26.0053619302949, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281363503, Length=373, Percent_Identity=26.0053619302949, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281363505, Length=373, Percent_Identity=26.0053619302949, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI78707236, Length=373, Percent_Identity=26.0053619302949, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24646386, Length=412, Percent_Identity=25, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24646388, Length=412, Percent_Identity=25, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 42304; Mature: 42304

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00331 MALIC_ENZYMES ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIKEKSLMVHKKFKGKLSIEGKIQVKNKEDLSIAYTPGVAEPCVKINEDKSLVYEYTMK
CCCCHHHHEEEECCCCEEEEEEEEEECCCCCCEEEECCCCCCCCEEECCCCEEEEEEEEC
GNTVAVVTNGTAVLGLGDIGPYAGLPVMEGKALLFKEFADIDSFPICIDSKDPEEIIKTV
CCEEEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHH
KLIAPGFGGINLEDIKAPECFYIEKKLKEELDIPVFHDDQHGTAIVVLAGIYNALRLVGK
HHHCCCCCCCCHHHCCCCCEEEHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHHHHH
KLEEAKIVINGAGSAGISICKLLLQAGAKSIIMCDKEGSLVKGNSNLNEAQKLIVEVTNK
HCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCHHHHHHHHHHCCCC
ENEKGTLKDVIKGKDVFIGVSAPNILTEEMVTSMNKDSIVFAMANPTPEIMPDKAKKAGA
CCCCCHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHCCC
RVVATGRSDFPNQINNVLVFPGIFRGALDVRSKVINEEMKLAASKAIASLVQDNELNEEY
EEEEECCCCCCHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IIPGAFDKRVAEVVAEEVKKVALKMRLSKL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDIKEKSLMVHKKFKGKLSIEGKIQVKNKEDLSIAYTPGVAEPCVKINEDKSLVYEYTMK
CCCCHHHHEEEECCCCEEEEEEEEEECCCCCCEEEECCCCCCCCEEECCCCEEEEEEEEC
GNTVAVVTNGTAVLGLGDIGPYAGLPVMEGKALLFKEFADIDSFPICIDSKDPEEIIKTV
CCEEEEEECCEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHH
KLIAPGFGGINLEDIKAPECFYIEKKLKEELDIPVFHDDQHGTAIVVLAGIYNALRLVGK
HHHCCCCCCCCHHHCCCCCEEEHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHHHHH
KLEEAKIVINGAGSAGISICKLLLQAGAKSIIMCDKEGSLVKGNSNLNEAQKLIVEVTNK
HCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCHHHHHHHHHHCCCC
ENEKGTLKDVIKGKDVFIGVSAPNILTEEMVTSMNKDSIVFAMANPTPEIMPDKAKKAGA
CCCCCHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHCCC
RVVATGRSDFPNQINNVLVFPGIFRGALDVRSKVINEEMKLAASKAIASLVQDNELNEEY
EEEEECCCCCCHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
IIPGAFDKRVAEVVAEEVKKVALKMRLSKL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644282 [H]