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The map label for this gene is tgt

Identifier: 170756970

GI number: 170756970

Start: 3312038

End: 3313168

Strand: Reverse

Name: tgt

Synonym: CLD_1472

Alternate gene names: 170756970

Gene position: 3313168-3312038 (Counterclockwise)

Preceding gene: 170757777

Following gene: 170755243

Centisome position: 83.7

GC content: 32.98

Gene sequence:

>1131_bases
ATGTACAAATTGTTAAAAAAATCAGGAAAGGCAAGACGAGGAGAATTTACGACTCCTCATGGGGTGATACAGACTCCTGT
ATTTATGAATGTAGGAACCCTTGCGGCCATAAAAGGGGCAGTATCATCTATGGATTTAAAAGAAATAGGATGCCAAGTTG
AGTTATCTAATACATACCATCTTCATTTAAGACCAGGAGATGAAGTTGTTAAAAAAATGGGTGGATTACATAAATTTATG
AATTGGGATAGGCCAATACTTACAGATTCAGGTGGATTTCAGGTATTTTCATTATCAAAAATAAGAAAAATACAAGAAGA
GGGAGTTTATTTTAACTCTCATATAGATGGAAGAAAAATATTTATGGGACCGGAAGAAAGTATGAGAATACAAAGCAATC
TAGCTTCAACTATAGCTATGGCTTTTGATGAATGTGTAGAAAATCCAGCACCAAGAGAGTATGTAGAAAAGTCCGTAGAA
AGAACAACAAGATGGTTACATAGATGTAAGGATGAAATGAATAGATTAAACTCTTTGCCAGATACTATAAATAATAAGCA
AATGTTATTTGGTATAAATCAAGGTGGAACTTATGAAGATATAAGAATAGAACATGCTAAAACTATAGCTAAAATGGATC
TAGATGGATATGCTATAGGGGGATTAGCAGTTGGAGAAAGTCATGAAGATATGTATAGAATAATAGATGCAGTGGTTCCC
CACCTTCCAGAAGATAAACCTATATATCTTATGGGAGTTGGGATACCAAGTAATATATTAGAAGCTGTAGATAGGGGTGT
AGATTTCTTTGACTGTGTTCTTCCTGCAAGAAATGGAAGACATGCACATGTTTTCACAAAAGAGGGCAAAATAAATTTAT
TGAATGCAAAATTTGAATTAGATGATAAACCTATTGATGAAGGTTGTCAGTGTCCAGCTTGTAAGCATTATACAAGGTCA
TATATAAGGCATTTATTTAAAGCAAAGGAAATGCTAGCTATGAGATTATGTGTTTTACATAACTTATATTTCTATAATAA
CTTAATGGAAGAAATAAGAGATGCCATTGATGGAGATTATTTTAAAGAATACAAAGAAAGAAAACTTAAAGAGTGGGGAG
GAAGAGCATAA

Upstream 100 bases:

>100_bases
GAATATAGATTCTTCTCTTTTGGAGATGCTATGTTTATTAAATAATATTATTAAGTAATTTTAATAGAAGCGTAATCCCT
AAGGAAAAAGAGGTGTAAGT

Downstream 100 bases:

>100_bases
AGAATTAAAAATAATTTTTGTGTATTAAATGCTATAATAAATTTTATATTTTAAATATAAAATAATATAAATATATCTAT
TAATTGTAAATTATCAATTA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MYKLLKKSGKARRGEFTTPHGVIQTPVFMNVGTLAAIKGAVSSMDLKEIGCQVELSNTYHLHLRPGDEVVKKMGGLHKFM
NWDRPILTDSGGFQVFSLSKIRKIQEEGVYFNSHIDGRKIFMGPEESMRIQSNLASTIAMAFDECVENPAPREYVEKSVE
RTTRWLHRCKDEMNRLNSLPDTINNKQMLFGINQGGTYEDIRIEHAKTIAKMDLDGYAIGGLAVGESHEDMYRIIDAVVP
HLPEDKPIYLMGVGIPSNILEAVDRGVDFFDCVLPARNGRHAHVFTKEGKINLLNAKFELDDKPIDEGCQCPACKHYTRS
YIRHLFKAKEMLAMRLCVLHNLYFYNNLMEEIRDAIDGDYFKEYKERKLKEWGGRA

Sequences:

>Translated_376_residues
MYKLLKKSGKARRGEFTTPHGVIQTPVFMNVGTLAAIKGAVSSMDLKEIGCQVELSNTYHLHLRPGDEVVKKMGGLHKFM
NWDRPILTDSGGFQVFSLSKIRKIQEEGVYFNSHIDGRKIFMGPEESMRIQSNLASTIAMAFDECVENPAPREYVEKSVE
RTTRWLHRCKDEMNRLNSLPDTINNKQMLFGINQGGTYEDIRIEHAKTIAKMDLDGYAIGGLAVGESHEDMYRIIDAVVP
HLPEDKPIYLMGVGIPSNILEAVDRGVDFFDCVLPARNGRHAHVFTKEGKINLLNAKFELDDKPIDEGCQCPACKHYTRS
YIRHLFKAKEMLAMRLCVLHNLYFYNNLMEEIRDAIDGDYFKEYKERKLKEWGGRA
>Mature_376_residues
MYKLLKKSGKARRGEFTTPHGVIQTPVFMNVGTLAAIKGAVSSMDLKEIGCQVELSNTYHLHLRPGDEVVKKMGGLHKFM
NWDRPILTDSGGFQVFSLSKIRKIQEEGVYFNSHIDGRKIFMGPEESMRIQSNLASTIAMAFDECVENPAPREYVEKSVE
RTTRWLHRCKDEMNRLNSLPDTINNKQMLFGINQGGTYEDIRIEHAKTIAKMDLDGYAIGGLAVGESHEDMYRIIDAVVP
HLPEDKPIYLMGVGIPSNILEAVDRGVDFFDCVLPARNGRHAHVFTKEGKINLLNAKFELDDKPIDEGCQCPACKHYTRS
YIRHLFKAKEMLAMRLCVLHNLYFYNNLMEEIRDAIDGDYFKEYKERKLKEWGGRA

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=40.8450704225352, Blast_Score=289, Evalue=2e-78,
Organism=Homo sapiens, GI13375872, Length=254, Percent_Identity=27.9527559055118, Blast_Score=104, Evalue=1e-22,
Organism=Escherichia coli, GI1786607, Length=356, Percent_Identity=47.4719101123596, Blast_Score=356, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17544680, Length=374, Percent_Identity=40.1069518716578, Blast_Score=274, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI25146502, Length=138, Percent_Identity=28.2608695652174, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19920510, Length=365, Percent_Identity=41.3698630136986, Blast_Score=293, Evalue=9e-80,
Organism=Drosophila melanogaster, GI21355311, Length=228, Percent_Identity=26.3157894736842, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42913; Mature: 42913

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKLLKKSGKARRGEFTTPHGVIQTPVFMNVGTLAAIKGAVSSMDLKEIGCQVELSNTYH
CCHHHHHCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCHHHCCCEEEECCEEE
LHLRPGDEVVKKMGGLHKFMNWDRPILTDSGGFQVFSLSKIRKIQEEGVYFNSHIDGRKI
EEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHCCCEEECCCCCCEE
FMGPEESMRIQSNLASTIAMAFDECVENPAPREYVEKSVERTTRWLHRCKDEMNRLNSLP
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DTINNKQMLFGINQGGTYEDIRIEHAKTIAKMDLDGYAIGGLAVGESHEDMYRIIDAVVP
CCCCCCEEEEEECCCCCCHHEEHHHHHHHHHCCCCCEEECCEEECCCHHHHHHHHHHHHC
HLPEDKPIYLMGVGIPSNILEAVDRGVDFFDCVLPARNGRHAHVFTKEGKINLLNAKFEL
CCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEECCCEEEEEEEEEC
DDKPIDEGCQCPACKHYTRSYIRHLFKAKEMLAMRLCVLHNLYFYNNLMEEIRDAIDGDY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
FKEYKERKLKEWGGRA
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MYKLLKKSGKARRGEFTTPHGVIQTPVFMNVGTLAAIKGAVSSMDLKEIGCQVELSNTYH
CCHHHHHCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHCCCHHHCCCEEEECCEEE
LHLRPGDEVVKKMGGLHKFMNWDRPILTDSGGFQVFSLSKIRKIQEEGVYFNSHIDGRKI
EEECCHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEHHHHHHHHHCCCEEECCCCCCEE
FMGPEESMRIQSNLASTIAMAFDECVENPAPREYVEKSVERTTRWLHRCKDEMNRLNSLP
EECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DTINNKQMLFGINQGGTYEDIRIEHAKTIAKMDLDGYAIGGLAVGESHEDMYRIIDAVVP
CCCCCCEEEEEECCCCCCHHEEHHHHHHHHHCCCCCEEECCEEECCCHHHHHHHHHHHHC
HLPEDKPIYLMGVGIPSNILEAVDRGVDFFDCVLPARNGRHAHVFTKEGKINLLNAKFEL
CCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEECCCEEEEEEEEEC
DDKPIDEGCQCPACKHYTRSYIRHLFKAKEMLAMRLCVLHNLYFYNNLMEEIRDAIDGDY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
FKEYKERKLKEWGGRA
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA