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The map label for this gene is rluB

Identifier: 170756858

GI number: 170756858

Start: 1933890

End: 1934600

Strand: Reverse

Name: rluB

Synonym: CLD_2827

Alternate gene names: 170756858

Gene position: 1934600-1933890 (Counterclockwise)

Preceding gene: 170756923

Following gene: 170756014

Centisome position: 48.88

GC content: 26.16

Gene sequence:

>711_bases
ATGGAAGAAAGATTACAAAAATATATGGCCTCTTGTGGAGTTGCTTCTAGAAGAAAATGTGAGGATATTATTTTAGAAGG
GCGAGTAGAAGTAAATGGTATTAAAATAAAAGAATTAGGTACAAAAATTATATCTGGAAAAGACATTATAAAAGTAGATG
GGAATATTATAGAACCAGAAGAAAATAAAATTTACATTTTACTTAATAAACCAAAAGGGTACGTATGTACTGCTAAAGAT
GAAAAGGGCAGAAAAACTATTTTAGATTTAGTAAAAGTTAAAGAAAGAATATACCCTATAGGTAGGTTAGATATGGATAC
ATCAGGTATAATAATACTAACCAATGACGGGGAAATATACAATAAGATTATTCATCCTAAATTTGAAAAAATAAAGACTT
ATAAGGCTAAAGTAAAAGGTACTATAAAAAAAGACCATATAGAGTTATTTAAAAAAGGTATAGATATAGGAGATTACTTA
ACAGCTCCAGCAACATTAGAAATTTTGAAAATAAATAAGGATACTTCTGAAATAATAATAAAAATTCACGAAGGTAAAAA
TAGACAAGTAAGAAGAATGTGTAAATCTATAGGTCATGCTGTTATAGATTTAGATAGGATTTCTATAGGTAATATAACAA
AAAGTGATTTGAAATTAGGTGAATGGAGATATTTAACTAATAAGGAAATAGATTATATAACAAAGATATAA

Upstream 100 bases:

>100_bases
TACTCTAAAAAGTTCGTCATAATTTCCTTAAATTTTGACTTTTTGTATAAAACCTATACAATGGTATTGTTACAGTAAAA
TATAATATAAAGGTGATTTT

Downstream 100 bases:

>100_bases
ATTAAATCTAAAAGCAAATAAGGCTGTTAAGAACTTACCAATAATATATTTAAAACCTTTTAAGATTCATTAAAAAATGA
TGAATAATTTCAAAAATATA

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MEERLQKYMASCGVASRRKCEDIILEGRVEVNGIKIKELGTKIISGKDIIKVDGNIIEPEENKIYILLNKPKGYVCTAKD
EKGRKTILDLVKVKERIYPIGRLDMDTSGIIILTNDGEIYNKIIHPKFEKIKTYKAKVKGTIKKDHIELFKKGIDIGDYL
TAPATLEILKINKDTSEIIIKIHEGKNRQVRRMCKSIGHAVIDLDRISIGNITKSDLKLGEWRYLTNKEIDYITKI

Sequences:

>Translated_236_residues
MEERLQKYMASCGVASRRKCEDIILEGRVEVNGIKIKELGTKIISGKDIIKVDGNIIEPEENKIYILLNKPKGYVCTAKD
EKGRKTILDLVKVKERIYPIGRLDMDTSGIIILTNDGEIYNKIIHPKFEKIKTYKAKVKGTIKKDHIELFKKGIDIGDYL
TAPATLEILKINKDTSEIIIKIHEGKNRQVRRMCKSIGHAVIDLDRISIGNITKSDLKLGEWRYLTNKEIDYITKI
>Mature_236_residues
MEERLQKYMASCGVASRRKCEDIILEGRVEVNGIKIKELGTKIISGKDIIKVDGNIIEPEENKIYILLNKPKGYVCTAKD
EKGRKTILDLVKVKERIYPIGRLDMDTSGIIILTNDGEIYNKIIHPKFEKIKTYKAKVKGTIKKDHIELFKKGIDIGDYL
TAPATLEILKINKDTSEIIIKIHEGKNRQVRRMCKSIGHAVIDLDRISIGNITKSDLKLGEWRYLTNKEIDYITKI

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=229, Percent_Identity=37.9912663755458, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1788510, Length=232, Percent_Identity=34.051724137931, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787524, Length=247, Percent_Identity=32.7935222672065, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI87081838, Length=177, Percent_Identity=32.7683615819209, Blast_Score=107, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 26968; Mature: 26968

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERLQKYMASCGVASRRKCEDIILEGRVEVNGIKIKELGTKIISGKDIIKVDGNIIEPE
CHHHHHHHHHHCCCCCHHHHHHHEEECEEEECCEEEHHHCCEEECCCEEEEECCEEECCC
ENKIYILLNKPKGYVCTAKDEKGRKTILDLVKVKERIYPIGRLDMDTSGIIILTNDGEIY
CCEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHH
NKIIHPKFEKIKTYKAKVKGTIKKDHIELFKKGIDIGDYLTAPATLEILKINKDTSEIII
HHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEE
KIHEGKNRQVRRMCKSIGHAVIDLDRISIGNITKSDLKLGEWRYLTNKEIDYITKI
EEECCCCHHHHHHHHHHCCEEEEEECEEECCCCHHHCCCCCEEEECCCCCCCEECC
>Mature Secondary Structure
MEERLQKYMASCGVASRRKCEDIILEGRVEVNGIKIKELGTKIISGKDIIKVDGNIIEPE
CHHHHHHHHHHCCCCCHHHHHHHEEECEEEECCEEEHHHCCEEECCCEEEEECCEEECCC
ENKIYILLNKPKGYVCTAKDEKGRKTILDLVKVKERIYPIGRLDMDTSGIIILTNDGEIY
CCEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHH
NKIIHPKFEKIKTYKAKVKGTIKKDHIELFKKGIDIGDYLTAPATLEILKINKDTSEIII
HHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEE
KIHEGKNRQVRRMCKSIGHAVIDLDRISIGNITKSDLKLGEWRYLTNKEIDYITKI
EEECCCCHHHHHHHHHHCCEEEEEECEEECCCCHHHCCCCCEEEECCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]