| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is banIIIM [H]
Identifier: 170756850
GI number: 170756850
Start: 3332742
End: 3334475
Strand: Reverse
Name: banIIIM [H]
Synonym: CLD_1450
Alternate gene names: 170756850
Gene position: 3334475-3332742 (Counterclockwise)
Preceding gene: 170754771
Following gene: 170756243
Centisome position: 84.24
GC content: 22.2
Gene sequence:
>1734_bases ATGAATGCATTTAAAGATAATATCGAAAAAATTTTTAATATATTAATATCTCCTATAAATAGTATTTACAAATATGAAGC TATTAATAACTTTAAATATAAGTTATCTATTGGTAAAAATGAGAATATATCCTTAAAATATTATGAATTTATAAAGGGTA TAAAAGAAACTGGAGTTATATATACTCCACAAGAAATTTCTAATTACATGATAGAAAATACTATAAATAAAGAGGATGTA ATAAATAATCCCTTTATAAAAATATTAGATCCTTCCTGTGGCTGTGGAAATATTTTGATTCCTTGTTTTTTTTATTTAAA AAATATATTTGAAGAAAATCTGAAAGAGATAAATAAAAAAAACAATATAAATTTAGAAAAACAATATATAAGTAAGCATA TATTAGATAATAATTTATATGGATTTGATATCGATACAATAGCTATAAAAATATTGATGATGGATTTGTTTTATTTAACA GGGTATTATAACAAAAATAATTTTAAGAAAAAAGATTTTTTGATAGAAGATATAAATAATAACTTTGATATATATATAGG GAATCCACCTTATGTTGGACATAAATCTGTAGATAAAGAATATTCAATGTTATTAAAAGGGAAATATGGATACGTATATA AGGATAAAGGGGATATATCTTATTGTTTTTTTATAAATGCACTAAATTATTCTAACATAAATAGCAAAATAACTTTTATA ACATCAAGATATTTTATGGAGTCTAAAAGCGGGCATAATCTAAGAAAATACTTAAAAGAAAACTGTAATATATATAAAAT ATTAGATTTCTATGGTATAAGACCCTTTAAGGCTGTAGGTATAGATCCAGCCATAATATTTATAGATAGAAATATTAGTA ATAAAGTGGAAATTATAAAGCCTTGCAGATATGAGAAAGTAAAAATGGGTTTATTTTTTAATAATGAAGATAAGTACGAA AAATTCTATGTACATATGTCAGAGCTAAAACAAGATGGATGGGTTTTGATAGATGATGGTAGTAGAGATATAATAAATAA GATAGAGAATAAAACCAATAAAACTTTAGGAGAAATATGTACAAGTTATCAGGGAATTATTACCGGATGTGATAAAGCTT TTATAGTTGATGAAAAAACTATAAAAAAGGAAAATTTGGAGAGGAGTATAATAAAGCCTTGGATAAAAAGTAGTTATATA AATAGAGAAAAAATTAATTTTAGAGATAGCTTTATAATATATTCAGATTTAATAGAAAATGTAAAAAAGTATCCTAATAT AATAAGGCATATTGAAAAATATAAGGATAAATTAGAAAATAGGCGAGAATGCAAGAAGAAGGTTAGAAAGTGGTATGAGT TGCAATGGGGAAGAAAATTCGATATATTTGAAGATAAAAAAATAATATTTCCTTATAAGGCTAGTAAGAATAAATTTTAT CTAGATAAAAAAAACGCATATTTTAGTGCAGATGTATATGCTTTGACCATAAATAAAGAAGAAGTCATAGATTATAATAG TTTATTAATTATTTTAAACAGTGCTATATATGAGTTTTATTTTAAGACTTTTGGTAAAAAATTAGGTGGTAGTTTATATG AGTATTACCCTAATACATTAATGAAATTAAAGATACCAATGATTAAGATAAATAAAGAAGAGGATTTATATAAATATTTT AATTTAAATGATAATGAAATAAAATTTATACAAAATAAGTTGCTAATTAAGTAA
Upstream 100 bases:
>100_bases AAGCTAAAACACAAGAAATAACTTTTTTGTTCATAAGTAAATCACCTATCCTTTTCTATGTTATACTTTTTATAGAATAA TAAAGATTGTGGTGAGTAAT
Downstream 100 bases:
>100_bases AAAGTCACCATATCATAATTTTTTTATTATTTTTAATAAGTAATAAGGTTTTTGTTTGAGCTACAAATATTTCTTCCTTA GAAGTTTTTATAACTTCTTC
Product: modification methylase family protein
Products: NA
Alternate protein names: M.BanIII; Adenine-specific methyltransferase BanIII [H]
Number of amino acids: Translated: 577; Mature: 577
Protein sequence:
>577_residues MNAFKDNIEKIFNILISPINSIYKYEAINNFKYKLSIGKNENISLKYYEFIKGIKETGVIYTPQEISNYMIENTINKEDV INNPFIKILDPSCGCGNILIPCFFYLKNIFEENLKEINKKNNINLEKQYISKHILDNNLYGFDIDTIAIKILMMDLFYLT GYYNKNNFKKKDFLIEDINNNFDIYIGNPPYVGHKSVDKEYSMLLKGKYGYVYKDKGDISYCFFINALNYSNINSKITFI TSRYFMESKSGHNLRKYLKENCNIYKILDFYGIRPFKAVGIDPAIIFIDRNISNKVEIIKPCRYEKVKMGLFFNNEDKYE KFYVHMSELKQDGWVLIDDGSRDIINKIENKTNKTLGEICTSYQGIITGCDKAFIVDEKTIKKENLERSIIKPWIKSSYI NREKINFRDSFIIYSDLIENVKKYPNIIRHIEKYKDKLENRRECKKKVRKWYELQWGRKFDIFEDKKIIFPYKASKNKFY LDKKNAYFSADVYALTINKEEVIDYNSLLIILNSAIYEFYFKTFGKKLGGSLYEYYPNTLMKLKIPMIKINKEEDLYKYF NLNDNEIKFIQNKLLIK
Sequences:
>Translated_577_residues MNAFKDNIEKIFNILISPINSIYKYEAINNFKYKLSIGKNENISLKYYEFIKGIKETGVIYTPQEISNYMIENTINKEDV INNPFIKILDPSCGCGNILIPCFFYLKNIFEENLKEINKKNNINLEKQYISKHILDNNLYGFDIDTIAIKILMMDLFYLT GYYNKNNFKKKDFLIEDINNNFDIYIGNPPYVGHKSVDKEYSMLLKGKYGYVYKDKGDISYCFFINALNYSNINSKITFI TSRYFMESKSGHNLRKYLKENCNIYKILDFYGIRPFKAVGIDPAIIFIDRNISNKVEIIKPCRYEKVKMGLFFNNEDKYE KFYVHMSELKQDGWVLIDDGSRDIINKIENKTNKTLGEICTSYQGIITGCDKAFIVDEKTIKKENLERSIIKPWIKSSYI NREKINFRDSFIIYSDLIENVKKYPNIIRHIEKYKDKLENRRECKKKVRKWYELQWGRKFDIFEDKKIIFPYKASKNKFY LDKKNAYFSADVYALTINKEEVIDYNSLLIILNSAIYEFYFKTFGKKLGGSLYEYYPNTLMKLKIPMIKINKEEDLYKYF NLNDNEIKFIQNKLLIK >Mature_577_residues MNAFKDNIEKIFNILISPINSIYKYEAINNFKYKLSIGKNENISLKYYEFIKGIKETGVIYTPQEISNYMIENTINKEDV INNPFIKILDPSCGCGNILIPCFFYLKNIFEENLKEINKKNNINLEKQYISKHILDNNLYGFDIDTIAIKILMMDLFYLT GYYNKNNFKKKDFLIEDINNNFDIYIGNPPYVGHKSVDKEYSMLLKGKYGYVYKDKGDISYCFFINALNYSNINSKITFI TSRYFMESKSGHNLRKYLKENCNIYKILDFYGIRPFKAVGIDPAIIFIDRNISNKVEIIKPCRYEKVKMGLFFNNEDKYE KFYVHMSELKQDGWVLIDDGSRDIINKIENKTNKTLGEICTSYQGIITGCDKAFIVDEKTIKKENLERSIIKPWIKSSYI NREKINFRDSFIIYSDLIENVKKYPNIIRHIEKYKDKLENRRECKKKVRKWYELQWGRKFDIFEDKKIIFPYKASKNKFY LDKKNAYFSADVYALTINKEEVIDYNSLLIILNSAIYEFYFKTFGKKLGGSLYEYYPNTLMKLKIPMIKINKEEDLYKYF NLNDNEIKFIQNKLLIK
Specific function: This methylase recognizes the double-stranded sequence ATCGAT, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the BanIII endonuclease [H]
COG id: COG1002
COG function: function code V; Type II restriction enzyme, methylase subunits
Gene ontology:
Cell location: Cytoplasmic
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003356 - InterPro: IPR002052 - InterPro: IPR002296 [H]
Pfam domain/function: PF02384 N6_Mtase [H]
EC number: =2.1.1.72 [H]
Molecular weight: Translated: 68695; Mature: 68695
Theoretical pI: Translated: 9.46; Mature: 9.46
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 3.3 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 3.3 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNAFKDNIEKIFNILISPINSIYKYEAINNFKYKLSIGKNENISLKYYEFIKGIKETGVI CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEHHHHHHCHHHCCEE YTPQEISNYMIENTINKEDVINNPFIKILDPSCGCGNILIPCFFYLKNIFEENLKEINKK ECHHHHHHHHHHCCCCHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC NNINLEKQYISKHILDNNLYGFDIDTIAIKILMMDLFYLTGYYNKNNFKKKDFLIEDINN CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCEECCC NFDIYIGNPPYVGHKSVDKEYSMLLKGKYGYVYKDKGDISYCFFINALNYSNINSKITFI CEEEEECCCCCCCCCCCCHHHHHHEECCCCEEEECCCCEEEEEEEECCCCCCCCCEEEEE TSRYFMESKSGHNLRKYLKENCNIYKILDFYGIRPFKAVGIDPAIIFIDRNISNKVEIIK EEEHHHCCCCCCHHHHHHHCCCCEEEEEHHHCCCCEEECCCCEEEEEEECCCCCCEEEEC PCRYEKVKMGLFFNNEDKYEKFYVHMSELKQDGWVLIDDGSRDIINKIENKTNKTLGEIC CCCCCEEEEEEEECCCCCHHHHEEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH TSYQGIITGCDKAFIVDEKTIKKENLERSIIKPWIKSSYINREKINFRDSFIIYSDLIEN HHCCHHEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHH VKKYPNIIRHIEKYKDKLENRRECKKKVRKWYELQWGRKFDIFEDKKIIFPYKASKNKFY HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEECCCCEEE LDKKNAYFSADVYALTINKEEVIDYNSLLIILNSAIYEFYFKTFGKKLGGSLYEYYPNTL EECCCCEEEEEEEEEEECHHHHCCCCHHEEHHHHHHHHHHHHHHHHHHCCHHHHHCCCCE MKLKIPMIKINKEEDLYKYFNLNDNEIKFIQNKLLIK EEEEEEEEEECCCCCHHHHCCCCCCHHHHHHHHHCCC >Mature Secondary Structure MNAFKDNIEKIFNILISPINSIYKYEAINNFKYKLSIGKNENISLKYYEFIKGIKETGVI CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEHHHHHHCHHHCCEE YTPQEISNYMIENTINKEDVINNPFIKILDPSCGCGNILIPCFFYLKNIFEENLKEINKK ECHHHHHHHHHHCCCCHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC NNINLEKQYISKHILDNNLYGFDIDTIAIKILMMDLFYLTGYYNKNNFKKKDFLIEDINN CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCEECCCCCCCCCCCCEECCC NFDIYIGNPPYVGHKSVDKEYSMLLKGKYGYVYKDKGDISYCFFINALNYSNINSKITFI CEEEEECCCCCCCCCCCCHHHHHHEECCCCEEEECCCCEEEEEEEECCCCCCCCCEEEEE TSRYFMESKSGHNLRKYLKENCNIYKILDFYGIRPFKAVGIDPAIIFIDRNISNKVEIIK EEEHHHCCCCCCHHHHHHHCCCCEEEEEHHHCCCCEEECCCCEEEEEEECCCCCCEEEEC PCRYEKVKMGLFFNNEDKYEKFYVHMSELKQDGWVLIDDGSRDIINKIENKTNKTLGEIC CCCCCEEEEEEEECCCCCHHHHEEEHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH TSYQGIITGCDKAFIVDEKTIKKENLERSIIKPWIKSSYINREKINFRDSFIIYSDLIEN HHCCHHEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHH VKKYPNIIRHIEKYKDKLENRRECKKKVRKWYELQWGRKFDIFEDKKIIFPYKASKNKFY HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEEEEEEECCCCEEE LDKKNAYFSADVYALTINKEEVIDYNSLLIILNSAIYEFYFKTFGKKLGGSLYEYYPNTL EECCCCEEEEEEEEEEECHHHHCCCCHHEEHHHHHHHHHHHHHHHHHHCCHHHHHCCCCE MKLKIPMIKINKEEDLYKYFNLNDNEIKFIQNKLLIK EEEEEEEEEECCCCCHHHHCCCCCCHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 1368640 [H]